JP6321637B2 - 白血球数の測定方法及び測定装置 - Google Patents
白血球数の測定方法及び測定装置 Download PDFInfo
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- JP6321637B2 JP6321637B2 JP2015520692A JP2015520692A JP6321637B2 JP 6321637 B2 JP6321637 B2 JP 6321637B2 JP 2015520692 A JP2015520692 A JP 2015520692A JP 2015520692 A JP2015520692 A JP 2015520692A JP 6321637 B2 JP6321637 B2 JP 6321637B2
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Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261668377P | 2012-07-05 | 2012-07-05 | |
| US61/668,377 | 2012-07-05 | ||
| PCT/US2013/049367 WO2014008424A1 (en) | 2012-07-05 | 2013-07-03 | Method and apparatus for determining white blood cell counts |
| US13/935,154 | 2013-07-03 | ||
| US13/935,154 US9096885B2 (en) | 2012-07-05 | 2013-07-03 | Method and apparatus for determining white blood cell counts |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015522165A JP2015522165A (ja) | 2015-08-03 |
| JP2015522165A5 JP2015522165A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 2016-07-28 |
| JP6321637B2 true JP6321637B2 (ja) | 2018-05-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015520692A Expired - Fee Related JP6321637B2 (ja) | 2012-07-05 | 2013-07-03 | 白血球数の測定方法及び測定装置 |
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Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430028A (zh) | 2010-09-16 | 2013-12-04 | 通用医疗公司 | 供诊断用的红细胞动力学 |
| CN104541149B (zh) | 2012-07-05 | 2017-03-01 | 贝克曼考尔特公司 | 用于确定白血细胞计数的方法和装置 |
| WO2014144826A1 (en) | 2013-03-15 | 2014-09-18 | Beckman Coulter, Inc. | Systems and methods for panel design in flow cytometry |
| DE102013216362A1 (de) * | 2013-08-19 | 2015-02-19 | Siemens Healthcare Diagnostics Products Gmbh | Analyseverfahren zur Klassifikationsunterstützung |
| CA2969912A1 (en) * | 2014-12-10 | 2016-06-16 | Neogenomics Laboratories, Inc. | Automated flow cytometry analysis method and system |
| US10264515B2 (en) * | 2014-12-22 | 2019-04-16 | Qualcomm Incorporated | Enhanced access network query protocol (ANQP) signaling to scale to support large numbers of service providers at an access point (AP) |
| CN106687810B (zh) * | 2014-12-31 | 2019-10-22 | 深圳迈瑞生物医疗电子股份有限公司 | 一种非诊断目的的有核红细胞报警方法、装置及流式细胞分析仪 |
| CN104732229B (zh) * | 2015-03-16 | 2018-04-27 | 华南理工大学 | 一种用于宫颈涂片图像中重叠细胞的分割方法 |
| US10955423B2 (en) | 2015-12-15 | 2021-03-23 | The General Hospital Corporation | Methods of estimating blood glucose and related systems |
| KR102411950B1 (ko) * | 2016-01-28 | 2022-06-22 | 베크만 컬터, 인코포레이티드 | 감염 검출 및 감별 시스템들 및 방법들 |
| CN107202903B (zh) * | 2016-03-18 | 2020-04-10 | 深圳迈瑞生物医疗电子股份有限公司 | 样本分析仪及其样本分析方法 |
| WO2017177192A1 (en) * | 2016-04-07 | 2017-10-12 | The General Hospital Corporation | White blood cell population dynamics |
| WO2018049064A1 (en) * | 2016-09-08 | 2018-03-15 | Abbott Laboratories | Automated body fluid analysis |
| WO2018231835A1 (en) * | 2017-06-14 | 2018-12-20 | CytoChip, Inc. | Devices and methods for cell analysis |
| EP3644041B1 (en) * | 2017-06-20 | 2022-11-02 | Shenzhen Mindray Bio-Medical Electronics Co., Ltd. | Method and device for identifying platelet aggregation, and cell analyzer |
| WO2019055520A1 (en) | 2017-09-13 | 2019-03-21 | Graftworx, Inc. | SYSTEMS AND METHODS FOR DETECTION OF PERIPROTETIC INFECTION |
| WO2019083844A1 (en) | 2017-10-23 | 2019-05-02 | Cytochip Inc. | DEVICES AND METHOD FOR MEASURING TARGET ANALYTES AND PARTICLES |
| US11852640B2 (en) | 2017-10-27 | 2023-12-26 | Beckman Coulter, Inc. | Hematology analyzers and methods of operation |
| AU2018385767A1 (en) * | 2017-12-15 | 2020-06-11 | Gastroklenz Inc. | Sensor monitoring system for in-dwelling catheter based treatments |
| WO2019204804A1 (en) | 2018-04-20 | 2019-10-24 | Beckman Coulter, Inc. | Sepsis infection determination systems and methods |
| US11521706B2 (en) | 2018-04-20 | 2022-12-06 | Beckman Coulter, Inc. | Testing and representing suspicion of sepsis |
| EP3789751B1 (en) | 2018-04-28 | 2024-06-26 | Shenzhen Mindray Bio-Medical Electronics Co., Ltd. | Method and system for determining platelet concentration |
| CN111684263A (zh) | 2018-04-28 | 2020-09-18 | 深圳迈瑞生物医疗电子股份有限公司 | 血液分析系统、血液分析仪、血液分析方法及存储介质 |
| CN111656161B (zh) | 2018-04-28 | 2024-02-20 | 深圳迈瑞生物医疗电子股份有限公司 | 样本分析仪异常的报警方法、系统及存储介质 |
| CN111989032B (zh) | 2018-06-15 | 2024-07-19 | 拜克门寇尔特公司 | 存在母细胞标志时确定败血症的方法 |
| CN108663306B (zh) * | 2018-07-27 | 2024-02-09 | 北京指真生物科技有限公司 | 一种流式细胞分析装置及补偿方法 |
| WO2021011349A1 (en) | 2019-07-12 | 2021-01-21 | Beckman Coulter, Inc. | Method of detecting sepsis using vital signs, including systolic blood pressure, hematology parameters, and combinations thereof |
| US11796447B2 (en) | 2019-07-12 | 2023-10-24 | Beckman Coulter, Inc. | Systems and methods for using cell granularitry in evaluating immune response to infection |
| US12019040B2 (en) | 2019-09-30 | 2024-06-25 | Cornell University | System and devices for monitoring cell-containing materials and methods of their use |
| CN112986104B (zh) * | 2019-12-17 | 2025-05-13 | 爱科来株式会社 | 测定装置和测定方法 |
| JP7660123B2 (ja) | 2019-12-27 | 2025-04-10 | ベックマン コールター， インコーポレイテッド | サンプル調製器具 |
| EP4365570A1 (en) * | 2022-11-04 | 2024-05-08 | Horiba, Ltd. | Measurement method, measurement system, and test reagent kit |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58115346A (ja) * | 1981-12-29 | 1983-07-09 | Shimadzu Corp | 血球種類識別装置 |
| JPH0664053B2 (ja) * | 1986-11-27 | 1994-08-22 | 東亜医用電子株式会社 | フロ−サイトメトリ−による白血球の分類方法 |
| KR970007077B1 (ko) * | 1987-03-13 | 1997-05-02 | 코울터 일렉트로닉스 인커퍼레이티드 | 광산란 기술을 이용한 다중-부분식별 분석 방법 |
| DE69434942T2 (de) * | 1993-02-25 | 2007-11-29 | Abbott Laboratories, Abbott Park | Mehrzweckreagenzsystem zur schnellen lysierung von vollblutproben |
| ATE236397T1 (de) | 1993-05-14 | 2003-04-15 | Coulter Int Corp | Retikulozyt bestimmungsverfahren und geraet, das lichtstreuungstechniken verwendet |
| US5656499A (en) * | 1994-08-01 | 1997-08-12 | Abbott Laboratories | Method for performing automated hematology and cytometry analysis |
| BR9611136A (pt) | 1995-10-23 | 1999-09-14 | Cytometrics Inc | Processo e aparelho para análise de imagem refletida |
| US5733784A (en) * | 1995-11-20 | 1998-03-31 | Abbott Laboratories | Reagent system and method for the differentiation and identification of reticulocytes |
| US6043066A (en) * | 1997-09-04 | 2000-03-28 | Mangano; Joseph A. | Cell separation using electric fields |
| US5874310A (en) * | 1997-11-21 | 1999-02-23 | Coulter International Corp. | Method for differentiation of nucleated red blood cells |
| US6228652B1 (en) | 1999-02-16 | 2001-05-08 | Coulter International Corp. | Method and apparatus for analyzing cells in a whole blood sample |
| BR0009420A (pt) * | 1999-03-31 | 2002-04-09 | Bayer Ag | Método de detecção de diluição simples e canal único |
| US6744245B2 (en) | 2001-07-27 | 2004-06-01 | Coulter International Corp. | Particle count correction method and apparatus |
| US6916658B2 (en) | 2001-07-27 | 2005-07-12 | Beckman Coulter, Inc. | Method for measurement of immature granulocytes |
| EP1412740B1 (en) * | 2001-07-27 | 2015-07-08 | Beckman Coulter, Inc. | Method for measurement of nucleated red blood cells |
| US7198953B2 (en) * | 2003-10-12 | 2007-04-03 | Beckman Coulter, Inc. | Method of using a reference control composition for measurement of nucleated red blood cells |
| EP1682865B1 (en) * | 2003-10-12 | 2018-08-15 | Beckman Coulter, Inc. | Method of using a reference control composition for measurement of nucleated red blood cells |
| US7208319B2 (en) | 2004-02-10 | 2007-04-24 | Beckman Coulter, Inc. | Method of measurement of nucleated red blood cells |
| WO2005076995A2 (en) * | 2004-02-10 | 2005-08-25 | Beckman Coulter, Inc. | Method of measurement of nucleated red blood cells |
| US7390662B2 (en) | 2005-11-09 | 2008-06-24 | Beckman Coulter, Inc. | Method and apparatus for performing platelet measurement |
| WO2006083969A2 (en) * | 2005-02-01 | 2006-08-10 | Amnis Corporation | Blood analysis using a flow imaging cytometer |
| CN101305087A (zh) | 2005-09-15 | 2008-11-12 | 阿尔特弥斯康复公司 | 用于磁富集细胞和其他颗粒的装置和方法 |
| JP4690163B2 (ja) * | 2005-09-28 | 2011-06-01 | シスメックス株式会社 | 血液撮像装置、血液撮像方法、処理装置、及び撮像本体装置 |
| US7796797B2 (en) | 2005-09-28 | 2010-09-14 | Sysmex Corporation | Apparatus for obtaining an image of a blood cell and method for obtaining an image of a blood cell |
| US7661326B2 (en) | 2006-10-26 | 2010-02-16 | Beckman Coulter, Inc. | Apparatus for aspirating and dispensing liquids in an automated analyzer |
| US8094299B2 (en) | 2008-07-24 | 2012-01-10 | Beckman Coulter, Inc. | Transducer module |
| US8512977B2 (en) | 2008-09-19 | 2013-08-20 | Beckman Coulter, Inc. | Analyzing reticulocytes |
| US8603773B2 (en) | 2008-09-19 | 2013-12-10 | Beckman Coulter | Method and system for analyzing a blood sample |
| US8417012B2 (en) | 2008-11-04 | 2013-04-09 | Beckman Coulter, Inc. | Non-linear histogram segmentation for particle analysis |
| ES2796655T3 (es) | 2008-11-14 | 2020-11-27 | Beckman Coulter Inc | Celdas de flujo óptico monolíticas y método de fabricación |
| US9464978B2 (en) | 2009-03-04 | 2016-10-11 | Beckman Coulter, Inc. | Cross-instrument method and system for cell population discrimination |
| US8906309B2 (en) * | 2009-04-27 | 2014-12-09 | Abbott Laboratories | Method for discriminating red blood cells from white blood cells by using forward scattering from a laser in an automated hematology analyzer |
| US8911669B2 (en) * | 2009-08-24 | 2014-12-16 | Abbott Laboratories | Method for flagging a sample |
| CN104541149B (zh) | 2012-07-05 | 2017-03-01 | 贝克曼考尔特公司 | 用于确定白血细胞计数的方法和装置 |
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