JP6267785B2 - Cmp−アセチルノイラミン酸ヒドロキシラーゼタゲッティングベクター、そのベクターが導入された異種間移植用形質転換動物及びその製造方法 - Google Patents
Cmp−アセチルノイラミン酸ヒドロキシラーゼタゲッティングベクター、そのベクターが導入された異種間移植用形質転換動物及びその製造方法 Download PDFInfo
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- C12Y114/18002—CMP-N-acetylneuraminate monooxygenase (1.14.18.2)
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Description
CMAH5’arm−PGKneopolyA−3’arm vectorの構築
5’armは、先ず、XbaI制限酵素siteを含んでいるsense primer(TCTAGACTCTCTATTTGGTGGCTCTGTTT)とEcoRI制限酵素siteを含んでいるanti−sense primer(GAATTCAGGAGTTTCTTCCTTTCTGTTTT)とを利用して、シカコミニアチュアミニ豚のゲノムDNAを利用してPCR増幅により確保した後、T−vectorにligationした。cloningされたDNAは、塩基配列決定によってCMAH遺伝子領域であることを確認した。
3’armは、シカコミニアチュアミニ豚のゲノムDNAを鋳型として利用し、XhoI制限酵素siteを含むsense primer(CTCGAGCCTACAACCCAGAATTTACTGCC)とKpnI制限酵素siteを含むanti−sense primer(GGTACCAACAGGGACCTGCCAAGAGGCCA)とを利用してPCR増幅してT−vectorにsubcloningした。cloningされたDNAは、塩基配列決定によってCMAH遺伝子領域であることを確認した。
CMAH 5’arm−PGKneopolyA−3’arm vectorの構築は、先ず、pKJ2 neo plasmidをEcoRIとXhoIで切断してPGKneoPolyA fragment(約2kb)を分離した後、EcoRIとXhoIで切断したpBluscriptII SK−のvectorにligationしてpBSK−PGKneoPoyA plasmidを確保した。5’の連結は、上記で確保された5plasmid(T−easy vector)からXbaIとEcoRIで切断して789bpのfragmentを確保した後、pBSK−PGKneoPoyA plasmidをXbaIとEcoRIで切断したvectorにligationしてpBSK−5’arm−PGKneoPolyA plasmidを構築した。そして、最終的に3’の連結は、上記でPCRによって確保した3plasmid(T−easy vector)をXhoIとKpnIで切断して763bp fragmentを確保した後、pBSK−5’arm−PGKneoPolyA plasmidをXhoIとKpnIで切断したvectorに挿入して、最終pBSK−5’arm−PGKneoPolyA−3’arm plasmidを構築した。
正方向Neo3−1:TCGTGCTTTACGGTATCGCCGCTCCCGATT、
逆方向ScAS3:AAGACTCCCACTTTAAAGGGTGGTGTGTAG
正方向ScS5:CCCTTCCATCCCACCCGTCCTCATCCTTAC、
逆方向CMAHR:ACTCTCTGTTTTCAGGCTGCTTGTT
正方向ScS5:CCCTTCCATCCCACCCGTCCTCATCCTTAC、
逆方向ScAS3:AAGACTCCCACTTTAAAGGGTGGTGTGTAG
Claims (5)
- 配列番号1に記載された塩基序列を有するCMP−アセチルノイラミン酸ヒドロキシラーゼ5’アーム(arm)、配列番号2に記載された塩基序列を有するPGKneopolyA、及び配列番号3に記載された塩基序列を有するCMP−アセチルノイラミン酸ヒドロキシラーゼ3’アーム(arm)を順次に含むCMP−アセチルノイラミン酸ヒドロキシラーゼタゲッティングベクター、並びに
ジンク−フィンガーヌクレアーゼベクター
を、ヒトを除く動物の細胞にトランスフェクションさせる段階を含むCMP−アセチルノイラミン酸ヒドロキシラーゼノックアウト細胞を製造する方法。 - 請求項1に記載の方法によって製造されたCMP−アセチルノイラミン酸ヒドロキシラーゼノックアウト細胞。
- 前記ノックアウト細胞は、寄託番号KCTC 12439BPで寄託されたことを特徴とする請求項2に記載のノックアウト細胞。
- 卵子を購入して体外で成熟させた後、卵子から核を除去した後、請求項2または請求項3の細胞を移植して融合を実施した後、生存した融合卵子のみを選別してヒトを除く動物である代理母の卵管に移植する段階を含むヒトを除いた動物を製造する方法。
- 請求項4の方法によって製造されたヒトを除いたクローン動物を飼育した後、その臓器を摘出する段階を含む免疫拒否反応を除去した移植用異種臓器の生産方法。
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KR10-2013-0047938 | 2013-04-30 | ||
KR20130047938 | 2013-04-30 | ||
PCT/KR2013/007592 WO2014178485A1 (ko) | 2013-04-30 | 2013-08-23 | Cmp-아세틸뉴라미닌산 히드록실라아제 타겟팅 벡터, 그 벡터가 도입된 이종간 이식용 형질전환 동물 및 그 제조방법 |
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JP2016518839A JP2016518839A (ja) | 2016-06-30 |
JP6267785B2 true JP6267785B2 (ja) | 2018-01-24 |
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JP2016511663A Active JP6267785B2 (ja) | 2013-04-30 | 2013-08-23 | Cmp−アセチルノイラミン酸ヒドロキシラーゼタゲッティングベクター、そのベクターが導入された異種間移植用形質転換動物及びその製造方法 |
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US (1) | US20160102319A1 (ja) |
EP (1) | EP2993234B1 (ja) |
JP (1) | JP6267785B2 (ja) |
KR (1) | KR101821873B1 (ja) |
CN (1) | CN106062196B (ja) |
DK (1) | DK2993234T3 (ja) |
ES (1) | ES2703056T3 (ja) |
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JP6992944B2 (ja) | 2015-12-28 | 2022-01-13 | サムソン エレクトロ-メカニックス カンパニーリミテッド. | 積層セラミック電子部品の製造方法 |
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WO2021247858A1 (en) * | 2020-06-03 | 2021-12-09 | Xenotherapeutics, Inc. | Selection and monitoring methods for xenotransplantation |
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US6235969B1 (en) | 1997-01-10 | 2001-05-22 | University Of Massachusetts | Cloning pigs using donor nuclei from non-quiescent differentiated cells |
US7291764B1 (en) | 1997-01-10 | 2007-11-06 | University of Massachusetts, a Public Institution of Higher Education of the Commonwealth of Massachusetts, as Represented by its Amherst Campus, Office of Vice Chancellor for Research at Amherst | Cloning pigs using non-quiescent differentiated donor cells or nuclei |
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KR20000034847A (ko) | 1998-11-17 | 2000-06-26 | 성재갑 | 인간 4-1비비 분자에 대한 인간화 항체 및 이를 포함하는 약학조성물 |
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KR100839172B1 (ko) | 2006-02-27 | 2008-06-17 | 김창현 | 이중 피펫, 이를 이용한 수핵난자의 탈핵 및 핵이식 방법및 체세포 복제동물의 생산방법 |
KR100807644B1 (ko) | 2007-03-19 | 2008-02-28 | 충남대학교산학협력단 | 체외수정 수정란 및 체세포 복제 수정란의 동시 이식에의한 돼지 생산 방법 |
KR101149475B1 (ko) | 2007-11-30 | 2012-05-25 | 한국생명공학연구원 | 이종 장기 이식용 미니 복제돼지 생산을 위한 유전자 조작된 세포주 및 그의 생산방법 |
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JP2014520533A (ja) * | 2011-06-30 | 2014-08-25 | シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニー | Cmp−n−アセチルノイラミン酸ヒドロキシラーゼおよび/または糖タンパク質アルファ−1,3−ガラクトシルトランスフェラーゼを欠損する細胞 |
KR101479671B1 (ko) * | 2011-11-02 | 2015-01-08 | 건국대학교 산학협력단 | Cmp-아세틸뉴라미닌산 히드록실라아제 타겟팅 벡터 및 그 응용 |
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ES2703056T3 (es) | 2019-03-06 |
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