JP6211703B2 - ヒト耐虫遺伝子およびそれによりコードされた抗Ｃｒｙ１Ｃ毒素イディオタイプ一本鎖抗体ならびに用途 - Google Patents

ヒト耐虫遺伝子およびそれによりコードされた抗Ｃｒｙ１Ｃ毒素イディオタイプ一本鎖抗体ならびに用途 Download PDF

Info

Publication number
JP6211703B2
JP6211703B2 JP2016535246A JP2016535246A JP6211703B2 JP 6211703 B2 JP6211703 B2 JP 6211703B2 JP 2016535246 A JP2016535246 A JP 2016535246A JP 2016535246 A JP2016535246 A JP 2016535246A JP 6211703 B2 JP6211703 B2 JP 6211703B2
Authority
JP
Japan
Prior art keywords
cry1c
single chain
toxin
antibody
well
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2016535246A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017505111A (ja
JP2017505111A5 (enExample
Inventor
▲劉▼▲賢▼金
徐重新
▲張▼霄
▲劉▼媛
▲謝▼雅晶
▲張▼存政
余向▲陽▼
王冬▲蘭▼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangsu Academy of Agricultural Sciences
Original Assignee
Jiangsu Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangsu Academy of Agricultural Sciences filed Critical Jiangsu Academy of Agricultural Sciences
Publication of JP2017505111A publication Critical patent/JP2017505111A/ja
Publication of JP2017505111A5 publication Critical patent/JP2017505111A5/ja
Application granted granted Critical
Publication of JP6211703B2 publication Critical patent/JP6211703B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
    • A01N57/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Gram-positive bacteria
    • C07K16/1278—Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/42—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins
    • C07K16/4208—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an idiotypic determinant on Ig
    • C07K16/4233—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an idiotypic determinant on Ig against anti-bacterial Ig
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
  • Catching Or Destruction (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2016535246A 2014-01-26 2015-01-09 ヒト耐虫遺伝子およびそれによりコードされた抗Ｃｒｙ１Ｃ毒素イディオタイプ一本鎖抗体ならびに用途 Expired - Fee Related JP6211703B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201410037240.9A CN103773776B (zh) 2014-01-26 2014-01-26 一种人源抗虫基因及其编码的抗Cry1C毒素独特型单链抗体与应用
CN201410037240.9 2014-01-26
PCT/CN2015/070422 WO2015109953A1 (zh) 2014-01-26 2015-01-09 一种人源抗虫基因及其编码的抗Cry1C毒素独特型单链抗体与应用

Publications (3)

Publication Number Publication Date
JP2017505111A JP2017505111A (ja) 2017-02-16
JP2017505111A5 JP2017505111A5 (enExample) 2017-09-14
JP6211703B2 true JP6211703B2 (ja) 2017-10-11

Family

ID=50566541

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2016535246A Expired - Fee Related JP6211703B2 (ja) 2014-01-26 2015-01-09 ヒト耐虫遺伝子およびそれによりコードされた抗Ｃｒｙ１Ｃ毒素イディオタイプ一本鎖抗体ならびに用途

Country Status (6)

Country Link
US (1) US9745385B2 (enExample)
EP (1) EP3048171B1 (enExample)
JP (1) JP6211703B2 (enExample)
CN (1) CN103773776B (enExample)
BR (1) BR112016016027B1 (enExample)
WO (1) WO2015109953A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103773775B (zh) 2014-01-26 2015-11-18 江苏省农业科学院 一种人源抗虫基因及其编码的抗Cry1B毒素独特型单链抗体与应用
CN104498501B (zh) * 2014-12-05 2017-07-18 江苏省农业科学院 一种人源杀虫基因及其编码杀虫肽与应用
CN104926940B (zh) * 2015-06-15 2018-02-09 江苏省农业科学院 一种人源杀虫蛋白及其制备方法与应用
CN105131123B (zh) * 2015-09-16 2018-11-27 江苏省农业科学院 一种具有杀虫活性的牛源化改型抗体及其制备方法与应用
CN105085683B (zh) * 2015-09-16 2018-11-27 江苏省农业科学院 一种具有杀虫活性的猪源化改型抗体及其制备方法与应用
EP3415010A1 (en) 2017-06-13 2018-12-19 Agrosavfe Nv Insect-controlling polypeptides and methods

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5942664A (en) * 1996-11-27 1999-08-24 Ecogen, Inc. Bacillus thuringiensis Cry1C compositions toxic to lepidopteran insects and methods for making Cry1C mutants
US7060491B1 (en) * 1999-11-18 2006-06-13 Pioneer Hi-Bred International, Inc. Polynucleotides encoding novel BT toxin receptors from lepidopteran insects
WO2007023782A1 (ja) * 2005-08-24 2007-03-01 Genecare Research Institute Co., Ltd. キラートキシン様タンパク質に対する中和抗体、およびその利用
CN102936598B (zh) * 2012-11-21 2014-04-16 江苏省农业科学院 抗Cry1Ac毒素单链抗体的编码基因及免疫PCR检测方法

Also Published As

Publication number Publication date
BR112016016027B1 (pt) 2022-02-08
EP3048171A1 (en) 2016-07-27
WO2015109953A1 (zh) 2015-07-30
US9745385B2 (en) 2017-08-29
JP2017505111A (ja) 2017-02-16
US20160215066A1 (en) 2016-07-28
CN103773776B (zh) 2015-11-18
BR112016016027A2 (pt) 2017-12-12
EP3048171A4 (en) 2017-03-01
EP3048171B1 (en) 2018-05-02
CN103773776A (zh) 2014-05-07

Similar Documents

Publication Publication Date Title
JP6236531B2 (ja) ヒト耐虫遺伝子およびそれによりコードされた抗Ｃｒｙ１Ａｂ毒素イディオタイプ一本鎖抗体ならびに用途
JP6196382B2 (ja) ヒト耐虫遺伝子およびそれによりコードされた抗Ｃｒｙ１Ｂ毒素イディオタイプ一本鎖抗体ならびに用途
JP6211702B2 (ja) ヒト殺虫遺伝子およびそれによりコードされた殺虫ペプチドならびに用途
JP2017505111A (ja) ヒト耐虫遺伝子およびそれによりコードされた抗Ｃｒｙ１Ｃ毒素イディオタイプ一本鎖抗体ならびに用途
CN116178512A (zh) 一种模拟Bt Cry毒素共性结构和功能的多肽及其编码基因与应用
CN105884892B (zh) 一种Bt Cry毒素广谱检测用蛋白及其编码基因与应用
CN118930646B (zh) 一种具备模拟Bt Cry1C毒素结构和杀虫功能的人源基因工程抗体及其突变体
US20090247422A1 (en) In SITU Induced Antigen Technology (ISIAT) for Identification of Polynucleotides Expressed during Infection or Colonization
CN113912684B (zh) 一种模拟Bt Cry1Ac蛋白关键抗虫功能表位的短肽及其编码基因与应用
CN105085683A (zh) 一种具有杀虫活性的猪源化改型抗体及其制备方法与应用
CN111909264B (zh) 一种人源化抗金黄色葡萄球菌生物活性肽及其编码基因与应用
CN121108329A (zh) 一种人源单域抗体抗虫基因c4d及其编码的蛋白质人源单域抗体c4d和应用

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160527

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20160527

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20161216

A975 Report on accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A971005

Effective date: 20170120

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170131

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170424

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170516

A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20170713

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20170822

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20170913

R150 Certificate of patent or registration of utility model

Ref document number: 6211703

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees