BR112016016027B1 - Gene de resistência a inseto derivado de humanos e anticorpo de cadeia única de idiotipo anti-toxina crylc codificado pelo mesmo e uso do mesmo - Google Patents

Gene de resistência a inseto derivado de humanos e anticorpo de cadeia única de idiotipo anti-toxina crylc codificado pelo mesmo e uso do mesmo Download PDF

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Publication number
BR112016016027B1
BR112016016027B1 BR112016016027-4A BR112016016027A BR112016016027B1 BR 112016016027 B1 BR112016016027 B1 BR 112016016027B1 BR 112016016027 A BR112016016027 A BR 112016016027A BR 112016016027 B1 BR112016016027 B1 BR 112016016027B1
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BR
Brazil
Prior art keywords
toxin
chain antibody
crylc
human
resistant gene
Prior art date
Application number
BR112016016027-4A
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English (en)
Portuguese (pt)
Other versions
BR112016016027A2 (pt
Inventor
Xianjin LIU
Chongxin XU
Xiao Zhang
Yuan Liu
Yajing XIE
Cunzheng ZHANG
Xiangyang YU
Donglan WANG
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Jiangsu Academy Of Agricultural Sciences
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Publication date
Application filed by Jiangsu Academy Of Agricultural Sciences filed Critical Jiangsu Academy Of Agricultural Sciences
Publication of BR112016016027A2 publication Critical patent/BR112016016027A2/pt
Publication of BR112016016027B1 publication Critical patent/BR112016016027B1/pt

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
    • A01N57/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Gram-positive bacteria
    • C07K16/1278—Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/42—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins
    • C07K16/4208—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an idiotypic determinant on Ig
    • C07K16/4233—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an idiotypic determinant on Ig against anti-bacterial Ig
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
  • Catching Or Destruction (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BR112016016027-4A 2014-01-26 2015-01-09 Gene de resistência a inseto derivado de humanos e anticorpo de cadeia única de idiotipo anti-toxina crylc codificado pelo mesmo e uso do mesmo BR112016016027B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201410037240.9A CN103773776B (zh) 2014-01-26 2014-01-26 一种人源抗虫基因及其编码的抗Cry1C毒素独特型单链抗体与应用
CN201410037240.9 2014-01-26
PCT/CN2015/070422 WO2015109953A1 (zh) 2014-01-26 2015-01-09 一种人源抗虫基因及其编码的抗Cry1C毒素独特型单链抗体与应用

Publications (2)

Publication Number Publication Date
BR112016016027A2 BR112016016027A2 (pt) 2017-12-12
BR112016016027B1 true BR112016016027B1 (pt) 2022-02-08

Family

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Family Applications (1)

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BR112016016027-4A BR112016016027B1 (pt) 2014-01-26 2015-01-09 Gene de resistência a inseto derivado de humanos e anticorpo de cadeia única de idiotipo anti-toxina crylc codificado pelo mesmo e uso do mesmo

Country Status (6)

Country Link
US (1) US9745385B2 (enExample)
EP (1) EP3048171B1 (enExample)
JP (1) JP6211703B2 (enExample)
CN (1) CN103773776B (enExample)
BR (1) BR112016016027B1 (enExample)
WO (1) WO2015109953A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103773775B (zh) 2014-01-26 2015-11-18 江苏省农业科学院 一种人源抗虫基因及其编码的抗Cry1B毒素独特型单链抗体与应用
CN104498501B (zh) * 2014-12-05 2017-07-18 江苏省农业科学院 一种人源杀虫基因及其编码杀虫肽与应用
CN104926940B (zh) * 2015-06-15 2018-02-09 江苏省农业科学院 一种人源杀虫蛋白及其制备方法与应用
CN105131123B (zh) * 2015-09-16 2018-11-27 江苏省农业科学院 一种具有杀虫活性的牛源化改型抗体及其制备方法与应用
CN105085683B (zh) * 2015-09-16 2018-11-27 江苏省农业科学院 一种具有杀虫活性的猪源化改型抗体及其制备方法与应用
EP3415010A1 (en) 2017-06-13 2018-12-19 Agrosavfe Nv Insect-controlling polypeptides and methods

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5942664A (en) * 1996-11-27 1999-08-24 Ecogen, Inc. Bacillus thuringiensis Cry1C compositions toxic to lepidopteran insects and methods for making Cry1C mutants
US7060491B1 (en) * 1999-11-18 2006-06-13 Pioneer Hi-Bred International, Inc. Polynucleotides encoding novel BT toxin receptors from lepidopteran insects
WO2007023782A1 (ja) * 2005-08-24 2007-03-01 Genecare Research Institute Co., Ltd. キラートキシン様タンパク質に対する中和抗体、およびその利用
CN102936598B (zh) * 2012-11-21 2014-04-16 江苏省农业科学院 抗Cry1Ac毒素单链抗体的编码基因及免疫PCR检测方法

Also Published As

Publication number Publication date
EP3048171A1 (en) 2016-07-27
WO2015109953A1 (zh) 2015-07-30
JP6211703B2 (ja) 2017-10-11
US9745385B2 (en) 2017-08-29
JP2017505111A (ja) 2017-02-16
US20160215066A1 (en) 2016-07-28
CN103773776B (zh) 2015-11-18
BR112016016027A2 (pt) 2017-12-12
EP3048171A4 (en) 2017-03-01
EP3048171B1 (en) 2018-05-02
CN103773776A (zh) 2014-05-07

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