JP6196382B2 - ヒト耐虫遺伝子およびそれによりコードされた抗Ｃｒｙ１Ｂ毒素イディオタイプ一本鎖抗体ならびに用途 - Google Patents

ヒト耐虫遺伝子およびそれによりコードされた抗Ｃｒｙ１Ｂ毒素イディオタイプ一本鎖抗体ならびに用途 Download PDF

Info

Publication number
JP6196382B2
JP6196382B2 JP2016535243A JP2016535243A JP6196382B2 JP 6196382 B2 JP6196382 B2 JP 6196382B2 JP 2016535243 A JP2016535243 A JP 2016535243A JP 2016535243 A JP2016535243 A JP 2016535243A JP 6196382 B2 JP6196382 B2 JP 6196382B2
Authority
JP
Japan
Prior art keywords
single chain
cry1b
toxin
antibody
well
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2016535243A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017505110A5 (enExample
JP2017505110A (ja
Inventor
▲劉▼▲賢▼金
徐重新
▲張▼霄
▲劉▼媛
▲謝▼雅晶
▲張▼存政
余向▲陽▼
王冬▲蘭▼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangsu Academy of Agricultural Sciences
Original Assignee
Jiangsu Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangsu Academy of Agricultural Sciences filed Critical Jiangsu Academy of Agricultural Sciences
Publication of JP2017505110A publication Critical patent/JP2017505110A/ja
Publication of JP2017505110A5 publication Critical patent/JP2017505110A5/ja
Application granted granted Critical
Publication of JP6196382B2 publication Critical patent/JP6196382B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/42—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins
    • C07K16/4208—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an idiotypic determinant on Ig
    • C07K16/4233—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an idiotypic determinant on Ig against anti-bacterial Ig
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Gram-positive bacteria
    • C07K16/1278—Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2016535243A 2014-01-26 2015-01-09 ヒト耐虫遺伝子およびそれによりコードされた抗Ｃｒｙ１Ｂ毒素イディオタイプ一本鎖抗体ならびに用途 Expired - Fee Related JP6196382B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201410037175.XA CN103773775B (zh) 2014-01-26 2014-01-26 一种人源抗虫基因及其编码的抗Cry1B毒素独特型单链抗体与应用
CN201410037175.X 2014-01-26
PCT/CN2015/070421 WO2015109952A1 (zh) 2014-01-26 2015-01-09 一种人源抗虫基因及其编码的抗Cry1B毒素独特型单链抗体与应用

Publications (3)

Publication Number Publication Date
JP2017505110A JP2017505110A (ja) 2017-02-16
JP2017505110A5 JP2017505110A5 (enExample) 2017-08-31
JP6196382B2 true JP6196382B2 (ja) 2017-09-13

Family

ID=50566540

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2016535243A Expired - Fee Related JP6196382B2 (ja) 2014-01-26 2015-01-09 ヒト耐虫遺伝子およびそれによりコードされた抗Ｃｒｙ１Ｂ毒素イディオタイプ一本鎖抗体ならびに用途

Country Status (6)

Country Link
US (1) US9770038B2 (enExample)
EP (1) EP3098316B1 (enExample)
JP (1) JP6196382B2 (enExample)
CN (1) CN103773775B (enExample)
BR (1) BR112016016014B1 (enExample)
WO (1) WO2015109952A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103773775B (zh) 2014-01-26 2015-11-18 江苏省农业科学院 一种人源抗虫基因及其编码的抗Cry1B毒素独特型单链抗体与应用
CN104498501B (zh) 2014-12-05 2017-07-18 江苏省农业科学院 一种人源杀虫基因及其编码杀虫肽与应用
CN104926940B (zh) * 2015-06-15 2018-02-09 江苏省农业科学院 一种人源杀虫蛋白及其制备方法与应用
CN105131123B (zh) * 2015-09-16 2018-11-27 江苏省农业科学院 一种具有杀虫活性的牛源化改型抗体及其制备方法与应用
CN105085683B (zh) * 2015-09-16 2018-11-27 江苏省农业科学院 一种具有杀虫活性的猪源化改型抗体及其制备方法与应用
US10981996B1 (en) 2020-06-01 2021-04-20 Abexxa Biologics, Inc. Antibodies targeting a complex comprising non-classical HLA-I and neoantigen and their methods of use
US10981997B1 (en) * 2020-06-01 2021-04-20 Abexxa Biologics, Inc. Antibodies targeting a complex comprising non-classical HLA-I and neoantigen and their methods of use
US11976120B2 (en) 2020-06-01 2024-05-07 Boehringer Ingelheim International Gmbh Antibodies targeting a complex comprising non-classical HLA-I and neoantigen and their methods of use
CN111909264B (zh) * 2020-08-05 2022-02-25 江苏省农业科学院 一种人源化抗金黄色葡萄球菌生物活性肽及其编码基因与应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE59205079D1 (de) * 1991-07-25 1996-02-29 Ciba Geigy Ag Immunologisches Nachweisverfahren
WO2007023782A1 (ja) * 2005-08-24 2007-03-01 Genecare Research Institute Co., Ltd. キラートキシン様タンパク質に対する中和抗体、およびその利用
PL1999141T3 (pl) * 2006-03-21 2011-10-31 Bayer Cropscience Nv Nowe geny kodujące białka owadobójcze
ATE528317T1 (de) * 2007-06-08 2011-10-15 Univ Mexico Nacional Autonoma Unterdrückung der resistenz von insekten gegen bacillus thuringiensis-cry-toxine unter verwendung von toxinen, die nicht den cadherin- rezeptor benötigen
CN103773776B (zh) 2014-01-26 2015-11-18 江苏省农业科学院 一种人源抗虫基因及其编码的抗Cry1C毒素独特型单链抗体与应用
CN103773775B (zh) 2014-01-26 2015-11-18 江苏省农业科学院 一种人源抗虫基因及其编码的抗Cry1B毒素独特型单链抗体与应用
CN103773774B (zh) 2014-01-26 2015-11-18 江苏省农业科学院 一种人源抗虫基因及其编码的抗Cry1Ab毒素独特型单链抗体与应用

Also Published As

Publication number Publication date
US9770038B2 (en) 2017-09-26
BR112016016014B1 (pt) 2022-06-07
CN103773775B (zh) 2015-11-18
EP3098316A9 (en) 2017-06-28
EP3098316A4 (en) 2017-06-21
EP3098316A1 (en) 2016-11-30
CN103773775A (zh) 2014-05-07
EP3098316B1 (en) 2019-09-18
US20170035055A1 (en) 2017-02-09
JP2017505110A (ja) 2017-02-16
BR112016016014A2 (pt) 2017-12-12
WO2015109952A1 (zh) 2015-07-30

Similar Documents

Publication Publication Date Title
JP6236531B2 (ja) ヒト耐虫遺伝子およびそれによりコードされた抗Ｃｒｙ１Ａｂ毒素イディオタイプ一本鎖抗体ならびに用途
JP2017505110A (ja) ヒト耐虫遺伝子およびそれによりコードされた抗Ｃｒｙ１Ｂ毒素イディオタイプ一本鎖抗体ならびに用途
JP6211703B2 (ja) ヒト耐虫遺伝子およびそれによりコードされた抗Ｃｒｙ１Ｃ毒素イディオタイプ一本鎖抗体ならびに用途
JP6211702B2 (ja) ヒト殺虫遺伝子およびそれによりコードされた殺虫ペプチドならびに用途
Xu et al. Establishment of a sensitive time-resolved fluoroimmunoassay for detection of Bacillus thuringiensis Cry1Ie toxin based nanobody from a phage display library
CN116178512A (zh) 一种模拟Bt Cry毒素共性结构和功能的多肽及其编码基因与应用
De Coninck et al. Fungal glucosylceramide-specific camelid single domain antibodies are characterized by broad spectrum antifungal activity
CN105884892B (zh) 一种Bt Cry毒素广谱检测用蛋白及其编码基因与应用
CN118930646B (zh) 一种具备模拟Bt Cry1C毒素结构和杀虫功能的人源基因工程抗体及其突变体
CN113912684B (zh) 一种模拟Bt Cry1Ac蛋白关键抗虫功能表位的短肽及其编码基因与应用
CN105085683A (zh) 一种具有杀虫活性的猪源化改型抗体及其制备方法与应用
CN111909264B (zh) 一种人源化抗金黄色葡萄球菌生物活性肽及其编码基因与应用
CN121108329A (zh) 一种人源单域抗体抗虫基因c4d及其编码的蛋白质人源单域抗体c4d和应用

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160527

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20160527

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20161216

A975 Report on accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A971005

Effective date: 20170120

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170131

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170424

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170516

A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20170713

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20170808

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20170817

R150 Certificate of patent or registration of utility model

Ref document number: 6196382

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees