CN103773775B - 一种人源抗虫基因及其编码的抗Cry1B毒素独特型单链抗体与应用 - Google Patents

一种人源抗虫基因及其编码的抗Cry1B毒素独特型单链抗体与应用 Download PDF

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CN103773775B
CN103773775B CN201410037175.XA CN201410037175A CN103773775B CN 103773775 B CN103773775 B CN 103773775B CN 201410037175 A CN201410037175 A CN 201410037175A CN 103773775 B CN103773775 B CN 103773775B
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chain antibody
cry1b
antibody
insect
idiotype single
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CN103773775A (zh
Inventor
刘贤金
徐重新
张霄
刘媛
谢雅晶
张存政
余向阳
王冬兰
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Jiangsu Academy of Agricultural Sciences
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Jiangsu Academy of Agricultural Sciences
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Priority to CN201410037175.XA priority Critical patent/CN103773775B/zh
Publication of CN103773775A publication Critical patent/CN103773775A/zh
Priority to US15/104,127 priority patent/US9770038B2/en
Priority to JP2016535243A priority patent/JP6196382B2/ja
Priority to BR112016016014-2A priority patent/BR112016016014B1/pt
Priority to EP15739989.0A priority patent/EP3098316B1/en
Priority to PCT/CN2015/070421 priority patent/WO2015109952A1/zh
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/42—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins
    • C07K16/4208—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an idiotypic determinant on Ig
    • C07K16/4233—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an idiotypic determinant on Ig against anti-bacterial Ig
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Gram-positive bacteria
    • C07K16/1278—Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201410037175.XA 2014-01-26 2014-01-26 一种人源抗虫基因及其编码的抗Cry1B毒素独特型单链抗体与应用 Active CN103773775B (zh)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CN201410037175.XA CN103773775B (zh) 2014-01-26 2014-01-26 一种人源抗虫基因及其编码的抗Cry1B毒素独特型单链抗体与应用
US15/104,127 US9770038B2 (en) 2014-01-26 2015-01-09 Human-derived insect-resistant gene and anti-CRY1B toxin idiotype single-chain antibody encoded thereby and application thereof
JP2016535243A JP6196382B2 (ja) 2014-01-26 2015-01-09 ヒト耐虫遺伝子およびそれによりコードされた抗Ｃｒｙ１Ｂ毒素イディオタイプ一本鎖抗体ならびに用途
BR112016016014-2A BR112016016014B1 (pt) 2014-01-26 2015-01-09 Gene resistente a inseto derivado de ser humano e anticorpo de cadeia única de idiótipo de anti-toxina cry1b codificado pelo mesmo e sua aplicação
EP15739989.0A EP3098316B1 (en) 2014-01-26 2015-01-09 Human-derived insect-resistant gene and anti-cry1b toxin idiotype single-chain antibody encoded thereby and application thereof
PCT/CN2015/070421 WO2015109952A1 (zh) 2014-01-26 2015-01-09 一种人源抗虫基因及其编码的抗Cry1B毒素独特型单链抗体与应用

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CN201410037175.XA CN103773775B (zh) 2014-01-26 2014-01-26 一种人源抗虫基因及其编码的抗Cry1B毒素独特型单链抗体与应用

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CN103773775A CN103773775A (zh) 2014-05-07
CN103773775B true CN103773775B (zh) 2015-11-18

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US (1) US9770038B2 (enExample)
EP (1) EP3098316B1 (enExample)
JP (1) JP6196382B2 (enExample)
CN (1) CN103773775B (enExample)
BR (1) BR112016016014B1 (enExample)
WO (1) WO2015109952A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103773775B (zh) 2014-01-26 2015-11-18 江苏省农业科学院 一种人源抗虫基因及其编码的抗Cry1B毒素独特型单链抗体与应用
CN104498501B (zh) 2014-12-05 2017-07-18 江苏省农业科学院 一种人源杀虫基因及其编码杀虫肽与应用
CN104926940B (zh) * 2015-06-15 2018-02-09 江苏省农业科学院 一种人源杀虫蛋白及其制备方法与应用
CN105131123B (zh) * 2015-09-16 2018-11-27 江苏省农业科学院 一种具有杀虫活性的牛源化改型抗体及其制备方法与应用
CN105085683B (zh) * 2015-09-16 2018-11-27 江苏省农业科学院 一种具有杀虫活性的猪源化改型抗体及其制备方法与应用
US10981996B1 (en) 2020-06-01 2021-04-20 Abexxa Biologics, Inc. Antibodies targeting a complex comprising non-classical HLA-I and neoantigen and their methods of use
US10981997B1 (en) * 2020-06-01 2021-04-20 Abexxa Biologics, Inc. Antibodies targeting a complex comprising non-classical HLA-I and neoantigen and their methods of use
US11976120B2 (en) 2020-06-01 2024-05-07 Boehringer Ingelheim International Gmbh Antibodies targeting a complex comprising non-classical HLA-I and neoantigen and their methods of use
CN111909264B (zh) * 2020-08-05 2022-02-25 江苏省农业科学院 一种人源化抗金黄色葡萄球菌生物活性肽及其编码基因与应用

Citations (1)

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CN101730712A (zh) * 2007-06-08 2010-06-09 墨西哥国立大学 使用不需要钙黏着蛋白受体的毒素遏制昆虫对苏云金芽孢杆菌cry毒素的抗性

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DE59205079D1 (de) * 1991-07-25 1996-02-29 Ciba Geigy Ag Immunologisches Nachweisverfahren
WO2007023782A1 (ja) * 2005-08-24 2007-03-01 Genecare Research Institute Co., Ltd. キラートキシン様タンパク質に対する中和抗体、およびその利用
PL1999141T3 (pl) * 2006-03-21 2011-10-31 Bayer Cropscience Nv Nowe geny kodujące białka owadobójcze
CN103773776B (zh) 2014-01-26 2015-11-18 江苏省农业科学院 一种人源抗虫基因及其编码的抗Cry1C毒素独特型单链抗体与应用
CN103773775B (zh) 2014-01-26 2015-11-18 江苏省农业科学院 一种人源抗虫基因及其编码的抗Cry1B毒素独特型单链抗体与应用
CN103773774B (zh) 2014-01-26 2015-11-18 江苏省农业科学院 一种人源抗虫基因及其编码的抗Cry1Ab毒素独特型单链抗体与应用

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CN101730712A (zh) * 2007-06-08 2010-06-09 墨西哥国立大学 使用不需要钙黏着蛋白受体的毒素遏制昆虫对苏云金芽孢杆菌cry毒素的抗性

Non-Patent Citations (2)

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抗苏云金芽孢杆菌 Cry1B 毒蛋白质单链抗体的筛选与鉴定;徐重新等;《江苏农业学报》;20121231;第28卷(第4期);摘要，第1.2.2-1.2.4节 *

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Publication number Publication date
US9770038B2 (en) 2017-09-26
BR112016016014B1 (pt) 2022-06-07
EP3098316A9 (en) 2017-06-28
EP3098316A4 (en) 2017-06-21
EP3098316A1 (en) 2016-11-30
CN103773775A (zh) 2014-05-07
EP3098316B1 (en) 2019-09-18
US20170035055A1 (en) 2017-02-09
JP6196382B2 (ja) 2017-09-13
JP2017505110A (ja) 2017-02-16
BR112016016014A2 (pt) 2017-12-12
WO2015109952A1 (zh) 2015-07-30

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