JP6180940B2 - エキソヌクレアーゼを用いた近接伸長アッセイ - Google Patents

エキソヌクレアーゼを用いた近接伸長アッセイ Download PDF

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JP6180940B2
JP6180940B2 JP2013550902A JP2013550902A JP6180940B2 JP 6180940 B2 JP6180940 B2 JP 6180940B2 JP 2013550902 A JP2013550902 A JP 2013550902A JP 2013550902 A JP2013550902 A JP 2013550902A JP 6180940 B2 JP6180940 B2 JP 6180940B2
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nucleic acid
proximity
domain
probe
analyte
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JP2014504880A (ja
JP2014504880A5 (https=
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フレドリクソン、シモン
ルンドベルグ、マーティン
エリクソン、アンナ
レンネル−ディケンズ、エマ
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オリンク プロテオミクス エービー
オリンク プロテオミクス エービー
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2013550902A 2011-01-31 2012-01-30 エキソヌクレアーゼを用いた近接伸長アッセイ Active JP6180940B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1101621.9A GB201101621D0 (en) 2011-01-31 2011-01-31 Method and product
GB1101621.9 2011-01-31
PCT/EP2012/051474 WO2012104261A1 (en) 2011-01-31 2012-01-30 Exonuclease enabled proximity extension assays

Related Child Applications (1)

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JP2017019754A Division JP6457564B2 (ja) 2011-01-31 2017-02-06 エキソヌクレアーゼを用いた近接伸長アッセイ

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JP2014504880A JP2014504880A (ja) 2014-02-27
JP2014504880A5 JP2014504880A5 (https=) 2015-03-12
JP6180940B2 true JP6180940B2 (ja) 2017-08-16

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JP2017019754A Active JP6457564B2 (ja) 2011-01-31 2017-02-06 エキソヌクレアーゼを用いた近接伸長アッセイ

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US (2) US9777315B2 (https=)
EP (2) EP3323896B1 (https=)
JP (2) JP6180940B2 (https=)
CN (1) CN103354840B (https=)
GB (1) GB201101621D0 (https=)
WO (1) WO2012104261A1 (https=)

Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011109379A1 (en) 2010-03-01 2011-09-09 Quanterix Corporation Methods and systems for extending dynamic range in assays for the detection of molecules or particles
US9952237B2 (en) 2011-01-28 2018-04-24 Quanterix Corporation Systems, devices, and methods for ultra-sensitive detection of molecules or particles
GB201101621D0 (en) * 2011-01-31 2011-03-16 Olink Ab Method and product
GB201201547D0 (en) 2012-01-30 2012-03-14 Olink Ab Method and product
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
CA2904181A1 (en) 2013-03-13 2014-10-09 Anahit Aghvanyan Sandwich immunoassay comprising anchoring reagent
WO2014144371A1 (en) 2013-03-15 2014-09-18 Fluidigm Corporation Simultaneous detection of target protein and target nucleic acids in a single cell
TWI553122B (zh) 2013-05-21 2016-10-11 Src公司 用於分子與蛋白質基於螢光之定量偵測的方法與系統
WO2015035087A1 (en) 2013-09-04 2015-03-12 Fluidigm Corporation Proximity assays for detecting nucleic acids and proteins in a single cell
GB201401885D0 (en) * 2014-02-04 2014-03-19 Olink Ab Proximity assay with detection based on hybridisation chain reaction (HCR)
US10112194B2 (en) 2014-04-14 2018-10-30 Q-Linea Ab Detection of microscopic objects
WO2015175856A1 (en) 2014-05-15 2015-11-19 Meso Scale Technologies, Llc. Improved assay methods
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
GB201414745D0 (en) 2014-08-19 2014-10-01 Articzymes As Exonucleases
CN105483115A (zh) * 2014-09-19 2016-04-13 深圳华大基因科技有限公司 一种用于单细胞全基因组扩增的试剂盒及其应用
CN104293930B (zh) * 2014-09-28 2016-07-06 南京诺唯赞生物科技有限公司 一种3’-5’外切酶活性测定方法
GB201518655D0 (en) 2015-10-21 2015-12-02 Olink Ab Method for generating proximity probes
AU2017259794B2 (en) 2016-05-02 2023-04-13 Encodia, Inc. Macromolecule analysis employing nucleic acid encoding
CN109154021A (zh) * 2016-05-25 2019-01-04 生物辐射实验室股份有限公司 数字邻近试验
US11208676B2 (en) 2016-05-27 2021-12-28 President And Fellows Of Harvard College Conditional primer extension for single-molecule detection
CN109789228B (zh) 2016-07-27 2022-10-21 斯坦福大学托管董事会 高度复用荧光成像
GB201614023D0 (en) 2016-08-16 2016-09-28 Olink Bioscience Ab Double-stranded circle probes
DK3548652T3 (da) 2016-12-01 2024-07-08 Nautilus Subsidiary Inc Fremgangsmåder til proteinanalyse
GB201700567D0 (en) 2017-01-12 2017-03-01 Genagon Therapeutics Ab Therapeutic agents
US20200264188A1 (en) 2017-09-13 2020-08-20 Progenity, Inc. Preeclampsia biomarkers and related systems and methods
MX2020004559A (es) * 2017-10-31 2020-10-05 Encodia Inc Kits para análisis utilizando codificación y/o etiqueta de ácido nucleico.
EP3704246A4 (en) 2017-10-31 2021-08-11 Encodia, Inc. PROCEDURES AND KITS USING NUCLEIC ACID ENCODING AND / OR MARKING
KR20250152680A (ko) 2017-12-29 2025-10-23 노틸러스 서브시디어리, 인크. 단백질 식별을 위한 디코딩 접근법
EP3749782B1 (en) 2018-02-05 2022-11-02 F. Hoffmann-La Roche AG Generation of single-stranded circular dna templates for single molecule sequencing
CN112941155B (zh) * 2018-05-14 2023-07-14 北京艾克伦医疗科技有限公司 具有茎环结构的dna引物对及其应用
JP7537748B2 (ja) * 2018-06-06 2024-08-21 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 核酸ライブラリを生成する方法ならびにそれを実施するための組成物およびキット
WO2019238944A1 (en) 2018-06-15 2019-12-19 Olink Proteomics Ab Biomarker panel for ovarian cancer
JP2022512058A (ja) * 2018-12-21 2022-02-02 イルミナ インコーポレイテッド ヌクレアーゼを利用したrna枯渇
US20220127600A1 (en) * 2019-01-09 2022-04-28 Qiagen Sciences, Llc Methods of Detecting Analytes and Compositions Thereof
AU2020232954B2 (en) 2019-03-01 2026-01-15 Meso Scale Technologies, Llc. Electrochemiluminescent labeled probes for use in immunoassay methods, methods using such and kits comprising same
AU2020233286B2 (en) 2019-03-01 2022-10-20 Mercy Bioanalytics, Inc. Systems, compositions, and methods for target entity detection
WO2020220013A1 (en) * 2019-04-26 2020-10-29 Arizona Board Of Regents On Behalf Of Arizona State University Proximity-driven activation of crispr-cas systems for detection of diverse molecular analytes
EP3963070A4 (en) 2019-04-30 2023-02-22 Encodia, Inc. METHODS OF PREPARATION OF ANALYTES AND RELATED KITS
EP3983806A4 (en) 2019-06-11 2023-06-21 Enable Biosciences Inc. MULTIPLEX COMPETITION TEST FOR PROFILING OF BINDING EPITOPES OF AFFINITY SUBSTANCES FOR CLINICAL DIAGNOSTICS
JP7727628B2 (ja) 2019-12-03 2025-08-21 アラマー バイオサイエンシーズ, インク. 核酸結合免疫サンドイッチアッセイ(nulisa)
EP4070113A4 (en) 2019-12-04 2023-12-20 Biora Therapeutics, Inc. ASSESSMENT OF PREECLAMPSIA USING FREE AND DISSOCIATE PLACENTAL GROWTH FACTOR TESTS
GB202004469D0 (en) * 2020-03-27 2020-05-13 Olink Proteomics Ab Controls for proximity detection assays
AU2021288692A1 (en) 2020-06-11 2023-02-02 Nautilus Subsidiary, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
JP2023545038A (ja) * 2020-10-06 2023-10-26 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 中和標的のシグナルオン検出のための方法、組成物、キット、およびシステム
GB202018503D0 (en) 2020-11-25 2021-01-06 Olink Proteomics Ab Analyte detection method employing concatamers
KR102573998B1 (ko) * 2020-12-30 2023-09-04 주식회사 스타노스 위양성 신호가 제거된 항원 검출방법 및 키트
EP4281774B1 (en) 2021-01-20 2025-11-26 Nautilus Subsidiary, Inc. Biomolecule quantitation
EP4281775B1 (en) 2021-01-21 2026-04-29 Nautilus Subsidiary, Inc. Systems and methods for biomolecule preparation
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
BR112023025731A2 (pt) * 2021-06-11 2024-02-27 Cellanome Inc Sistemas e métodos para a análise de amostras biológicas.
US20230035685A1 (en) * 2021-07-30 2023-02-02 10X Genomics, Inc. One step in situ rolling circle amplification assay
CA3227872A1 (en) 2021-09-09 2023-03-16 James Henry JOLY Characterization and localization of protein modifications
CA3227592A1 (en) 2021-09-22 2023-03-30 Gregory KAPP Methods and systems for determining polypeptide interactions
US20230149883A1 (en) 2021-11-03 2023-05-18 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
US12092578B2 (en) 2022-03-29 2024-09-17 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
WO2023212490A1 (en) 2022-04-25 2023-11-02 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
EP4544077A1 (en) 2022-06-21 2025-04-30 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
WO2024059655A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
EP4382534A1 (en) 2022-12-06 2024-06-12 Biomérieux Synthetic peptide for use in an immunomolecular assay
WO2024124073A1 (en) 2022-12-09 2024-06-13 Nautilus Subsidiary, Inc. A method comprising performing on a single-analyte array at least 50 cycles of a process
US20240201182A1 (en) 2022-12-15 2024-06-20 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
EP4655590A1 (en) 2023-01-27 2025-12-03 Biomerieux Modified immuno-molecular assay for faster biomarker detection
EP4689173A1 (en) 2023-03-24 2026-02-11 Nautilus Subsidiary, Inc. Improved transfer of nanoparticles to array surfaces
US20240394902A1 (en) 2023-05-24 2024-11-28 Nautilus Subsidiary, Inc. Systems and methods for machine vision
CN121463999A (zh) 2023-07-11 2026-02-03 欧邻科蛋白质组学公司 干燥试剂
US20250066841A1 (en) 2023-08-24 2025-02-27 Nautilus Subsidiary, Inc. Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions
WO2025111398A1 (en) 2023-11-20 2025-05-30 Alamar Biosciences, Inc. Reduction of non-cognate signal in multiplex proximity ligation assays
WO2025111396A1 (en) 2023-11-20 2025-05-30 Alamar Biosciences, Inc. Molecular barcode set and use thereof in multiplex proximity detection assays
WO2025111397A1 (en) 2023-11-20 2025-05-30 Alamar Biosciences, Inc. Use of autoinhibition standard curves in proximity assays
CN117343999B (zh) * 2023-12-05 2024-03-01 上海锐赛循益生物技术有限公司 基于左侧探针退火和右侧探针退火延伸的核酸扩增方法
US20250189519A1 (en) 2023-12-11 2025-06-12 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
WO2025137166A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
WO2025168224A1 (en) 2024-02-08 2025-08-14 Leica Microsystems Cms Gmbh Marker and method for analysing a biological sample
WO2025199005A1 (en) 2024-03-20 2025-09-25 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Biomarkers for identifying cancer patients at high risk for chemotherapy-induced cardiotoxicity
US20250298016A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
WO2025212338A1 (en) 2024-04-01 2025-10-09 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation
WO2025262178A1 (en) * 2024-06-18 2025-12-26 Olink Proteomics Ab System and method for detecting analytes in a sample

Family Cites Families (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU530410B2 (en) 1978-02-21 1983-07-14 Sintef Preparing aqueous emulsions
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4883867A (en) 1985-11-01 1989-11-28 Becton, Dickinson And Company Detection of reticulocytes, RNA or DNA
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5266684A (en) 1988-05-02 1993-11-30 The Reagents Of The University Of California Peptide mixtures
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5449603A (en) 1989-10-24 1995-09-12 Stratagene Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein
US5733523A (en) 1990-12-10 1998-03-31 Akzo Nobel N.V. Targeted delivery of a therapeutic entity using complementary oligonucleotides
ES2101116T3 (es) 1991-09-13 1997-07-01 Cytocell Ltd Metodo de deteccion de una secuencia de acidos nucleicos.
WO1993006482A1 (en) 1991-09-16 1993-04-01 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes
JP2001524926A (ja) 1991-09-18 2001-12-04 アフィマックス テクノロジーズ ナームロゼ フェンノートシャップ オリゴマーの雑多ライブラリーの集合体を合成する方法
US5639603A (en) 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
WO1993015226A1 (en) 1992-01-28 1993-08-05 President And Fellows Of Harvard College Method for nucleic acid hybridization using single-stranded dna binding protein
US5321130A (en) 1992-02-10 1994-06-14 Molecular Probes, Inc. Unsymmetrical cyanine dyes with a cationic side chain
US5541061A (en) 1992-04-29 1996-07-30 Affymax Technologies N.V. Methods for screening factorial chemical libraries
DE69334095T2 (de) 1992-07-17 2007-04-12 Dana-Farber Cancer Institute, Boston Verfahren zur intrazellulären Bindung von zielgerichteten Molekülen
US5288514A (en) 1992-09-14 1994-02-22 The Regents Of The University Of California Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support
US5565324A (en) 1992-10-01 1996-10-15 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US5721099A (en) 1992-10-01 1998-02-24 Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US5271099A (en) 1992-11-02 1993-12-21 Lin Ming Huei Sunshade cap with a double layer visor
US5410030A (en) 1993-04-05 1995-04-25 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes containing pyridinium moieties
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5440016A (en) 1993-06-18 1995-08-08 Torrey Pines Institute For Molecular Studies Peptides of the formula (KFmoc) ZZZ and their uses
US5851829A (en) 1993-07-16 1998-12-22 Dana-Farber Cancer Institute Method of intracellular binding of target molecules
US5635602A (en) 1993-08-13 1997-06-03 The Regents Of The University Of California Design and synthesis of bispecific DNA-antibody conjugates
US5593853A (en) 1994-02-09 1997-01-14 Martek Corporation Generation and screening of synthetic drug libraries
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5688997A (en) 1994-05-06 1997-11-18 Pharmacopeia, Inc. Process for preparing intermediates for a combinatorial dihydrobenzopyran library
US5525735A (en) 1994-06-22 1996-06-11 Affymax Technologies Nv Methods for synthesizing diverse collections of pyrrolidine compounds
US5549974A (en) 1994-06-23 1996-08-27 Affymax Technologies Nv Methods for the solid phase synthesis of thiazolidinones, metathiazanones, and derivatives thereof
US5463564A (en) 1994-09-16 1995-10-31 3-Dimensional Pharmaceuticals, Inc. System and method of automatically generating chemical compounds with desired properties
US5688696A (en) 1994-12-12 1997-11-18 Selectide Corporation Combinatorial libraries having a predetermined frequency of each species of test compound
SE504798C2 (sv) 1995-06-16 1997-04-28 Ulf Landegren Immunanalys och testkit med två reagens som kan tvärbindas om de adsorberats till analyten
DK0839152T3 (da) 1995-06-21 2002-07-08 Martek Biosciences Corp Kombinatoriske biblioteker af mærkede, biokemiske forbindelser og fremgangsmåde til fremstilling af disse
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US5731423A (en) 1996-03-21 1998-03-24 Transcell Technologies, Inc. Process for preparing sulfoxides
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
JP4060892B2 (ja) 1997-05-28 2008-03-12 ディサーナ リミテッド タンパク質のin vitroディスプレイ及び進化のための選択粒子としてのリボソーム複合体
EP0930370B1 (en) 1997-12-15 2007-08-08 CSL Behring GmbH Labeled primer for use in detection of target nucleic acids
US5989823A (en) 1998-09-18 1999-11-23 Nexstar Pharmaceuticals, Inc. Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
EP1985714B1 (en) 1998-03-25 2012-02-29 Olink AB Method and kit for detecting a target molecule employing at least two affinity probes and rolling circle replication of padlock probes
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
SE516272C2 (sv) 2000-02-18 2001-12-10 Ulf Landegren Metoder och kit för analytdetektion mha proximitets-probning
US6511809B2 (en) 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
US8013134B2 (en) 2001-11-23 2011-09-06 Olink Ab Kit for proximity probing with multivalent proximity probes
US7553619B2 (en) 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
US20050221349A1 (en) 2002-05-30 2005-10-06 Stuart Wilson Methods of detecting target molecules and molecular interactions
AU2004232976B2 (en) 2003-04-18 2011-02-10 Becton, Dickinson And Company Immuno-amplification
US7914987B2 (en) 2004-06-14 2011-03-29 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for use in analyte detection using proximity probes
DE602005023529D1 (de) 2004-11-03 2010-10-21 Iris Molecular Diagnostics Inc Homogener nachweis von analyten
US20070026430A1 (en) 2005-06-30 2007-02-01 Applera Corporation Proximity probing of target proteins comprising restriction and/or extension
US20080293051A1 (en) 2005-08-30 2008-11-27 Board Of Regents, The University Of Texas System proximity ligation assay
GB0605584D0 (en) 2006-03-20 2006-04-26 Olink Ab Method for analyte detection using proximity probes
US20080090238A1 (en) 2006-10-12 2008-04-17 Dan-Hui Dorothy Yang Increased sensitivity of proximity ligation assays
WO2009012220A2 (en) 2007-07-17 2009-01-22 Agilent Technologies Inc. Analyte detection assays
US7659069B2 (en) 2007-08-31 2010-02-09 Agilent Technologies, Inc. Binary signaling assay using a split-polymerase
EP2195465A4 (en) 2007-09-28 2010-12-22 Affomix Corp POLYNUCLEOTIDE SKELETERS FOR COMPLEXING PROTEINS
AU2009232716B2 (en) * 2008-03-31 2015-02-05 Tagcyx Biotechnologies Novel DNA capable of being amplified by PCR with high selectivity and high efficiency
US9040287B2 (en) * 2010-02-12 2015-05-26 Mediomics, Llc Molecular biosensors capable of signal amplification
GB201011971D0 (en) 2010-07-15 2010-09-01 Olink Ab Methods and product
GB201101621D0 (en) * 2011-01-31 2011-03-16 Olink Ab Method and product

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US10731206B2 (en) 2020-08-04
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US20180100189A1 (en) 2018-04-12
GB201101621D0 (en) 2011-03-16
CN103354840B (zh) 2016-06-08
US9777315B2 (en) 2017-10-03
CN103354840A (zh) 2013-10-16
US20140030721A1 (en) 2014-01-30
JP6457564B2 (ja) 2019-01-23
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