JP6118725B2 - 核酸合成のための方法およびデバイス - Google Patents

核酸合成のための方法およびデバイス Download PDF

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JP6118725B2
JP6118725B2 JP2013538904A JP2013538904A JP6118725B2 JP 6118725 B2 JP6118725 B2 JP 6118725B2 JP 2013538904 A JP2013538904 A JP 2013538904A JP 2013538904 A JP2013538904 A JP 2013538904A JP 6118725 B2 JP6118725 B2 JP 6118725B2
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oligonucleotides
support
construction
anchor
oligonucleotide
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JP2014504153A (ja
JP2014504153A5 (https=
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ジョセフ・ジェイコブソン
リ−ユン・エイ・クン
アンドリュー・カーク・ウィルソン
センティル・ラム
ダニエル・シンドラー
マイク・ハドソン
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Gen9 Inc
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Gen9 Inc
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Lubricants (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2013538904A 2010-11-12 2011-11-10 核酸合成のための方法およびデバイス Expired - Fee Related JP6118725B2 (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US41293710P 2010-11-12 2010-11-12
US61/412,937 2010-11-12
US41809510P 2010-11-30 2010-11-30
US61/418,095 2010-11-30
US201161466814P 2011-03-23 2011-03-23
US61/466,814 2011-03-23
US201161503722P 2011-07-01 2011-07-01
US61/503,722 2011-07-01
PCT/US2011/060243 WO2012078312A2 (en) 2010-11-12 2011-11-10 Methods and devices for nucleic acids synthesis

Publications (3)

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JP2014504153A JP2014504153A (ja) 2014-02-20
JP2014504153A5 JP2014504153A5 (https=) 2014-12-25
JP6118725B2 true JP6118725B2 (ja) 2017-04-19

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EP (4) EP3360963B1 (https=)
JP (1) JP6118725B2 (https=)
CN (1) CN103502448B (https=)
AU (1) AU2011338841B2 (https=)
CA (2) CA2817697C (https=)
ES (1) ES2548400T3 (https=)
IL (3) IL280133B2 (https=)
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Families Citing this family (99)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
US9187777B2 (en) 2010-05-28 2015-11-17 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
EP2633071B1 (en) 2010-10-27 2016-10-12 President and Fellows of Harvard College Compositions of toehold primer duplexes and methods of use
CN103502448B (zh) 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
WO2012106385A2 (en) 2011-01-31 2012-08-09 Apprise Bio, Inc. Methods of identifying multiple epitopes in cells
EP4737389A2 (en) 2011-05-27 2026-05-06 Oxford Nanopore Technologies PLC Coupling method
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
LT2944693T (lt) 2011-08-26 2019-08-26 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
DE102012100824A1 (de) * 2012-02-01 2013-09-05 Albert-Ludwigs-Universität Freiburg Gemultiplexte Digital PCR
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
WO2013153911A1 (ja) * 2012-04-12 2013-10-17 国立大学法人東京大学 核酸の定量方法、検出プローブ、検出プローブセット、及び核酸の検出方法
US20150329890A9 (en) * 2012-04-16 2015-11-19 Jingdong Tian Error correction in nucleic acid molecules
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
IL236303B (en) 2012-06-25 2022-07-01 Gen9 Inc Methods for high-throughput nucleic acid assembly and sequencing
DK2885408T5 (da) 2012-08-16 2024-10-14 Synthetic Genomics Inc Digital til biologisk konverter
US10273471B2 (en) * 2013-03-15 2019-04-30 Gen 9, Inc. Compositions and methods for multiplex nucleic acids synthesis
US9708658B2 (en) * 2013-03-19 2017-07-18 New England Biolabs, Inc. Enrichment of target sequences
SG11201600853UA (en) 2013-08-05 2016-03-30 Twist Bioscience Corp De novo synthesized gene libraries
CA2931533C (en) 2013-12-09 2023-08-08 Illumina, Inc. Methods and compositions for targeted nucleic acid sequencing
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
EP3102699B1 (en) 2014-02-07 2018-10-31 Qiagen Sciences, LLC Pcr primers
CA2958581C (en) * 2014-08-18 2023-04-04 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
US11639522B2 (en) 2015-01-30 2023-05-02 President And Fellows Of Harvard College Microscope-free imaging
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA2975855C (en) 2015-02-04 2025-09-23 Twist Bioscience Corporation SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS
GB201502645D0 (en) * 2015-02-17 2015-04-01 Touchlight Genetics Ltd Method
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US10689690B2 (en) 2015-08-13 2020-06-23 Centrillion Technology Holdings Corporation Library construction using Y-adapters and vanishing restriction sites
GB201516348D0 (en) * 2015-09-15 2015-10-28 Labgenius Ltd Compositions and methods for polynucleotide assembly
EP3350314A4 (en) 2015-09-18 2019-02-06 Twist Bioscience Corporation OLIGONUCLEIC ACID VARIANT LIBRARIES VARIANT AND SYNTHESIS THEREOF
KR102794025B1 (ko) 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
WO2017059399A1 (en) * 2015-10-01 2017-04-06 University Of Washington Multiplex pairwise assembly of dna oligonucleotides
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
EP3393525A4 (en) * 2015-12-21 2019-08-21 Gen9, Inc. METHODS AND COMPOSITIONS FOR ASSEMBLING NUCLEIC ACIDS
EP4239079B1 (en) 2016-02-17 2025-12-17 President And Fellows Of Harvard College Molecular programming tools
EP3491003A4 (en) * 2016-07-28 2020-03-25 Ginkgo Bioworks Inc. DEVICES AND METHODS FOR NUCLEIC ACID MANIPULATION
EP3500672A4 (en) 2016-08-22 2020-05-20 Twist Bioscience Corporation DE NOVO SYNTHETIZED NUCLEIC ACID LIBRARIES
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
US10538796B2 (en) * 2016-10-13 2020-01-21 Agilent Technologies, Inc. On-array ligation assembly
CN107937389B (zh) * 2016-10-13 2023-05-02 安捷伦科技有限公司 阵列上连接组装
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
JP7116062B2 (ja) 2017-01-10 2022-08-09 プレジデント アンド フェローズ オブ ハーバード カレッジ 多重化シグナル増幅
GB2559117B (en) * 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
EP4556433A3 (en) 2017-02-22 2025-08-06 Twist Bioscience Corporation Nucleic acid based data storage
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018217702A1 (en) * 2017-05-22 2018-11-29 Gen9, Inc. Device and method for nucleic acid manipulation
WO2018231872A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
GB2566986A (en) 2017-09-29 2019-04-03 Evonetix Ltd Error detection during hybridisation of target double-stranded nucleic acid
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
KR102804057B1 (ko) 2018-01-04 2025-05-07 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
EP3737755B1 (en) * 2018-01-12 2024-08-28 Camena Bioscience Limited Method for template-free geometric enzymatic nucleic acid synthesis
CN111630179B (zh) 2018-01-26 2024-07-19 哈佛学院院长及董事 邻近检测方法和组合物
CN112189055B (zh) 2018-03-22 2024-07-19 哈佛学院院长及董事 用于分子认证的方法和组合物
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
GB2574197B (en) * 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
GB201811811D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811810D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811813D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
SG11202012762WA (en) * 2018-12-05 2021-01-28 Illumina Cambridge Ltd Methods and compositions for cluster generation by bridge amplification
WO2020139871A1 (en) * 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
WO2020150143A2 (en) 2019-01-14 2020-07-23 Camena Bioscience Limited Compositions and methods for template-free geometric enzymatic nucleic acid synthesis
CN113631764A (zh) * 2019-02-21 2021-11-09 斯特拉托斯基因公司 用于固态合成在单分子测序中使用的可扩展聚合物的方法、组合物和装置
CN113766930B (zh) 2019-02-26 2025-07-22 特韦斯特生物科学公司 Glp1受体的变异核酸文库
JP2022522668A (ja) 2019-02-26 2022-04-20 ツイスト バイオサイエンス コーポレーション 抗体を最適化するための変異体核酸ライブラリ
GB201903056D0 (en) 2019-03-07 2019-04-24 Nuclera Nucleics Ltd Method of oligonucleotide synthesis
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
EP4034566A4 (en) 2019-09-23 2024-01-24 Twist Bioscience Corporation VARIANT NUCLEIC ACID BANKS FOR CRTH2
JP2022548783A (ja) 2019-09-23 2022-11-21 ツイスト バイオサイエンス コーポレーション 単一ドメイン抗体のバリアント核酸ライブラリー
GB201914282D0 (en) * 2019-10-03 2019-11-20 Stemnovate Ltd Method
EP4045646A4 (en) * 2019-10-18 2023-11-22 Encodia, Inc. AUTOMATED TREATMENT OF MACROMOLECULES FOR ANALYSIS AND ASSOCIATED APPARATUS
US20240271171A1 (en) * 2019-11-29 2024-08-15 Bgi Shenzhen Co., Ltd. Enzymatic synthesis of oligonucleotides
BR112022011235A2 (pt) 2019-12-09 2022-12-13 Twist Bioscience Corp Bibliotecas de variantes de ácido nucleico para receptores de adenosina
KR20220104057A (ko) * 2019-12-30 2022-07-25 위안디안 바이오랩스 컴퍼니 리미티드 효소를 이용한 핵산 서열 제조 장치 및 방법
US20220356500A1 (en) * 2019-12-30 2022-11-10 Yuandian Biolabs Co., Ltd. Method for preparing nucleic acid sequences using enzyme
MX2022013499A (es) 2020-04-27 2023-01-16 Twist Bioscience Corp Bibliotecas de ácidos nucleicos variantes para coronavirus.
EP4204460A4 (en) 2020-08-26 2024-09-25 Twist Bioscience Corporation METHODS AND COMPOSITIONS RELATING TO GLP1R VARIANTS
CN117043171A (zh) 2020-08-28 2023-11-10 特韦斯特生物科学公司 用于合成的装置和方法
WO2022051369A1 (en) * 2020-09-01 2022-03-10 Replay Holdings, Llc Methods and systems for nucleic acid synthesis
EP4229210A4 (en) 2020-10-19 2025-01-08 Twist Bioscience Corporation METHOD FOR THE SYNTHESIS OF OLIGONUCLEOTIDES USING BOUND NUCLEOTIDES
KR20230147617A (ko) 2021-01-21 2023-10-23 트위스트 바이오사이언스 코포레이션 아데노신 수용체에 관한 방법 및 조성물
EP4314075A4 (en) 2021-03-24 2025-04-09 Twist Bioscience Corporation VARIANT NUCLEIC ACID BANKS FOR CD3
WO2022235584A1 (en) 2021-05-03 2022-11-10 Twist Bioscience Corporation Variant nucleic acid libraries for glycans
US12201857B2 (en) 2021-06-22 2025-01-21 Twist Bioscience Corporation Methods and compositions relating to covid antibody epitopes
WO2023023285A2 (en) 2021-08-19 2023-02-23 Twist Bioscience Corporation Methods and compositions relating to covalently closed nucleic acids
EP4392554A1 (en) 2021-08-23 2024-07-03 DNA Script Method for generating double-stranded nucleic acid
US20240384319A1 (en) * 2021-09-17 2024-11-21 The University Of Manchester Synthesis of Oligonucleotides
EP4460516A2 (en) 2022-01-03 2024-11-13 Twist Bioscience Corporation Bispecific sars-cov-2 antibodies and methods of use
US20230257789A1 (en) * 2022-02-11 2023-08-17 Microsoft Technology Licensing, Llc Enzymatic oligonucleotide assembly using hairpins and enzymatic cleavage
WO2024054431A1 (en) * 2022-09-06 2024-03-14 Switchback Systems, Inc. Solid state polynucleotide assembly
EP4735621A1 (en) * 2023-06-30 2026-05-06 DNA Script Nucleic acid synthesis on reusable support

Family Cites Families (365)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4500707A (en) 1980-02-29 1985-02-19 University Patents, Inc. Nucleosides useful in the preparation of polynucleotides
US5096825A (en) 1983-01-12 1992-03-17 Chiron Corporation Gene for human epidermal growth factor and synthesis and expression thereof
DE3301833A1 (de) 1983-01-20 1984-07-26 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Verfahren zur simultanen synthese mehrerer oligonocleotide an fester phase
DE3329892A1 (de) 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur herstellung von oligonucleotiden
US4888286A (en) 1984-02-06 1989-12-19 Creative Biomolecules, Inc. Production of gene and protein analogs through synthetic gene design using double stranded synthetic oligonucleotides
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
CA1293460C (en) 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5093251A (en) 1986-05-23 1992-03-03 California Institute Of Technology Cassette method of gene synthesis
US5017692A (en) 1986-09-04 1991-05-21 Schering Corporation Truncated human interleukin-a alpha
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US4999294A (en) 1987-12-17 1991-03-12 New England Biolabs, Inc. Method for producing the FokI restriction endonuclease and methylase
US5700637A (en) 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
WO1990000626A1 (en) 1988-07-14 1990-01-25 Baylor College Of Medicine Solid phase assembly and reconstruction of biopolymers
US5132215A (en) 1988-09-15 1992-07-21 Eastman Kodak Company Method of making double-stranded dna sequences
GB8822228D0 (en) 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
US5047524A (en) 1988-12-21 1991-09-10 Applied Biosystems, Inc. Automated system for polynucleotide synthesis and purification
US6008031A (en) 1989-05-12 1999-12-28 Duke University Method of analysis and manipulation of DNA utilizing mismatch repair systems
US5459039A (en) 1989-05-12 1995-10-17 Duke University Methods for mapping genetic mutations
US5556750A (en) 1989-05-12 1996-09-17 Duke University Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems
US5104789A (en) 1989-05-15 1992-04-14 The United States Of America, As Represented By The Secretary Of Agriculture Monoclonal antibodies which discriminate between strains of citrus tristeza virus
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US5104792A (en) 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
CA2036946C (en) 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
WO1992015694A1 (en) 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
US5512463A (en) 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
US5474796A (en) 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US5770358A (en) 1991-09-18 1998-06-23 Affymax Technologies N.V. Tagged synthetic oligomer libraries
US5639603A (en) 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
US5605662A (en) 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US6017696A (en) 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US5846708A (en) 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
US5384261A (en) 1991-11-22 1995-01-24 Affymax Technologies N.V. Very large scale immobilized polymer synthesis using mechanically directed flow paths
EP0675966B1 (en) 1992-02-19 2004-10-06 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5395750A (en) 1992-02-28 1995-03-07 Hoffmann-La Roche Inc. Methods for producing proteins which bind to predetermined antigens
US5356802A (en) 1992-04-03 1994-10-18 The Johns Hopkins University Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
US5916794A (en) 1992-04-03 1999-06-29 Johns Hopkins University Methods for inactivating target DNA and for detecting conformational change in a nucleic acid
GB9207381D0 (en) 1992-04-03 1992-05-13 Ici Plc Synthesis of oligonucleotides
US5750335A (en) 1992-04-24 1998-05-12 Massachusetts Institute Of Technology Screening for genetic variation
US5541061A (en) 1992-04-29 1996-07-30 Affymax Technologies N.V. Methods for screening factorial chemical libraries
US5639423A (en) 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
US5288514A (en) 1992-09-14 1994-02-22 The Regents Of The University Of California Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support
US5795714A (en) 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
US5368823A (en) 1993-02-11 1994-11-29 University Of Georgia Research Foundation, Inc. Automated synthesis of oligonucleotides
US5498531A (en) 1993-09-10 1996-03-12 President And Fellows Of Harvard College Intron-mediated recombinant techniques and reagents
US6150141A (en) 1993-09-10 2000-11-21 Trustees Of Boston University Intron-mediated recombinant techniques and reagents
US6027877A (en) 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
KR100491810B1 (ko) 1994-02-17 2005-10-11 맥시겐, 인크. 무작위단편화및재조립에의한dna돌연변이유발방법
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5514789A (en) 1994-04-21 1996-05-07 Barrskogen, Inc. Recovery of oligonucleotides by gas phase cleavage
EP0776330B1 (en) 1994-06-23 2003-08-20 Affymax Technologies N.V. Photolabile compounds and methods for their use
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US5871902A (en) 1994-12-09 1999-02-16 The Gene Pool, Inc. Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids
WO1996028248A1 (en) 1995-03-15 1996-09-19 City Of Hope Disposable reagent storage and delivery cartridge
CA2219136A1 (en) 1995-04-24 1996-10-31 Chromaxome Corp. Methods for generating and screening novel metabolic pathways
US5624711A (en) 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5700642A (en) 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5877280A (en) 1995-06-06 1999-03-02 The Mount Sinai School Of Medicine Of The City University Of New York Thermostable muts proteins
US6406847B1 (en) 1995-10-02 2002-06-18 The Board Of Trustees Of The Leland Stanford Junior University Mismatch repair detection
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
WO1997021837A1 (en) 1995-12-15 1997-06-19 Amersham Life Science, Inc. Methods for the detection and removal of mutant sequences that arise during enzymatic amplification using mismatch repair systems
US6261797B1 (en) 1996-01-29 2001-07-17 Stratagene Primer-mediated polynucleotide synthesis and manipulation techniques
US6013440A (en) 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US6242211B1 (en) 1996-04-24 2001-06-05 Terragen Discovery, Inc. Methods for generating and screening novel metabolic pathways
US5851804A (en) 1996-05-06 1998-12-22 Apollon, Inc. Chimeric kanamycin resistance gene
SE9602062D0 (sv) 1996-05-29 1996-05-29 Pharmacia Biotech Ab Method for detection of mutations
US6495318B2 (en) 1996-06-17 2002-12-17 Vectorobjects, Llc Method and kits for preparing multicomponent nucleic acid constructs
CA2258570A1 (en) 1996-06-17 1997-12-24 Biodynamics Associates Method and kits for preparing multicomponent nucleic acid constructs
ZA975891B (en) 1996-07-05 1998-07-23 Combimatrix Corp Electrochemical solid phase synthesis of polymers
JPH1066576A (ja) 1996-08-07 1998-03-10 Novo Nordisk As 突出末端を有する２本鎖ｄｎａ及びこれを用いたｄｎａのシャフリング方法
EP2034021A1 (en) 1996-08-23 2009-03-11 Peter Ruhdal Jensen Artificial promoter libraries for selected organisms and promoters derived from such libraries
US6444650B1 (en) 1996-10-01 2002-09-03 Geron Corporation Antisense compositions for detecting and inhibiting telomerase reverse transcriptase
US6110668A (en) 1996-10-07 2000-08-29 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Gene synthesis method
AU5156198A (en) 1996-10-29 1998-05-22 Xeotron Corporation Optical device utilizing optical waveguides and mechanical light-switches
DE69735112T2 (de) 1996-11-06 2006-09-07 Sequenom, Inc., San Diego Verfahren zur Analyse und Vorrichtung
IL120338A0 (en) 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Single step DNA fragments assembly
IL120339A0 (en) 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Improved DNA assembly method
US6410220B1 (en) 1997-02-28 2002-06-25 Nature Technology Corp Self-assembling genes, vectors and uses thereof
US6410225B1 (en) 1997-06-27 2002-06-25 Yale University Purification of oligomers
US6489141B1 (en) 1997-07-09 2002-12-03 The University Of Queensland Nucleic acid sequence and methods for selectively expressing a protein in a target cell or tissue
CN1265156A (zh) 1997-07-22 2000-08-30 拉普吉恩公司 核酸阵列上进行的扩增及其它酶反应
US6326489B1 (en) 1997-08-05 2001-12-04 Howard Hughes Medical Institute Surface-bound, bimolecular, double-stranded DNA arrays
US6031098A (en) 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
DE19736591A1 (de) 1997-08-22 1999-02-25 Peter Prof Dr Hegemann Verfahren zum Herstellen von Nukleinsäurepolymeren
WO1999010369A1 (en) 1997-08-28 1999-03-04 Thomas Jefferson University Compositions, kits, and methods for effecting adenine nucleotide modulation of dna mismatch recognition proteins
US6136568A (en) 1997-09-15 2000-10-24 Hiatt; Andrew C. De novo polynucleotide synthesis using rolling templates
PT1015576E (pt) 1997-09-16 2005-09-30 Egea Biosciences Llc Metodo para a sintese quimica completa e montagem de genes e de genomas
US6670127B2 (en) 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
JP2001519538A (ja) 1997-10-10 2001-10-23 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 核酸アレイのレプリカ増幅
US20020127552A1 (en) 1997-10-10 2002-09-12 Church George M Replica amplification of nucleic acid arrays
US6548021B1 (en) 1997-10-10 2003-04-15 President And Fellows Of Harvard College Surface-bound, double-stranded DNA protein arrays
US6177558B1 (en) 1997-11-13 2001-01-23 Protogene Laboratories, Inc. Method and composition for chemical synthesis using high boiling point organic solvents to control evaporation
US6042211A (en) 1997-11-25 2000-03-28 Hewlett-Packard Company Ink drop volume variance compensation for inkjet printing
AU1603199A (en) 1997-12-03 1999-06-16 Curagen Corporation Methods and devices for measuring differential gene expression
US6509156B1 (en) 1997-12-05 2003-01-21 Europaisches Laboratorium Fur Molekularoiologie (Embl) DNA cloning method relying on the E. coli recE/recT recombination system
JP3795751B2 (ja) 1997-12-11 2006-07-12 ユニバーシティ オブ サスカッチェワン ブタからの離乳後多全身系消耗症候群ウイルス
US6093302A (en) 1998-01-05 2000-07-25 Combimatrix Corporation Electrochemical solid phase synthesis
US20030050437A1 (en) 1998-01-05 2003-03-13 Montgomery Donald D. Electrochemical solid phase synthesis
US6150102A (en) 1998-02-03 2000-11-21 Lucent Technologies Inc. Method of generating nucleic acid oligomers of known composition
WO1999041007A2 (en) 1998-02-11 1999-08-19 University Of Houston Method and apparatus for chemical and biochemical reactions using photo-generated reagents
US6375903B1 (en) 1998-02-23 2002-04-23 Wisconsin Alumni Research Foundation Method and apparatus for synthesis of arrays of DNA probes
US5912129A (en) 1998-03-05 1999-06-15 Vinayagamoorthy; Thuraiayah Multi-zone polymerase/ligase chain reaction
DE19812103A1 (de) * 1998-03-19 1999-09-23 Bernauer Annette Verfahren zur Synthese von Nucleinsäuremolekülen
US6670605B1 (en) 1998-05-11 2003-12-30 Halliburton Energy Services, Inc. Method and apparatus for the down-hole characterization of formation fluids
US6271957B1 (en) 1998-05-29 2001-08-07 Affymetrix, Inc. Methods involving direct write optical lithography
EP0967217B1 (en) 1998-06-22 2005-12-21 Affymetrix, Inc. (a California Corporation) Reagents and methods for solid phase synthesis and display
US6846655B1 (en) 1998-06-29 2005-01-25 Phylos, Inc. Methods for generating highly diverse libraries
US6287825B1 (en) 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US5942609A (en) 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
DE19854946C2 (de) 1998-11-27 2002-01-03 Guenter Von Kiedrowski Klonieren und Kopieren an Oberflächen
US6358712B1 (en) 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
US20040005673A1 (en) 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
CA2360011A1 (en) 1999-01-05 2000-07-13 Trustees Of Boston University Ordered gene assembly
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US20030054390A1 (en) 1999-01-19 2003-03-20 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6372484B1 (en) 1999-01-25 2002-04-16 E.I. Dupont De Nemours And Company Apparatus for integrated polymerase chain reaction and capillary electrophoresis
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
CA2361191A1 (en) 1999-02-03 2000-08-10 The Children's Medical Center Corporation Gene repair involving the induction of double-stranded dna cleavage at a chromosomal target site
WO2000049142A1 (de) 1999-02-19 2000-08-24 Febit Ferrarius Biotechnology Gmbh Verfahren zur herstellung von polymeren
ATE296310T1 (de) 1999-03-08 2005-06-15 Metrigen Inc Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür
US6824866B1 (en) 1999-04-08 2004-11-30 Affymetrix, Inc. Porous silica substrates for polymer synthesis and assays
US6511849B1 (en) 1999-04-23 2003-01-28 The Sir Mortimer B. Davis - Jewish General Hospital Microarrays of biological materials
DE19925862A1 (de) * 1999-06-07 2000-12-14 Diavir Gmbh Verfahren zur Synthese von DNA-Fragmenten
US8137906B2 (en) 1999-06-07 2012-03-20 Sloning Biotechnology Gmbh Method for the synthesis of DNA fragments
US6355412B1 (en) 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
US6653151B2 (en) 1999-07-30 2003-11-25 Large Scale Proteomics Corporation Dry deposition of materials for microarrays using matrix displacement
US6613581B1 (en) 1999-08-26 2003-09-02 Caliper Technologies Corp. Microfluidic analytic detection assays, devices, and integrated systems
GB9920711D0 (en) 1999-09-03 1999-11-03 Hd Technologies Limited High dynamic range mass spectrometer
EP1235932A2 (en) 1999-10-08 2002-09-04 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
US6315958B1 (en) 1999-11-10 2001-11-13 Wisconsin Alumni Research Foundation Flow cell for synthesis of arrays of DNA probes and the like
US6800439B1 (en) 2000-01-06 2004-10-05 Affymetrix, Inc. Methods for improved array preparation
EP1130113A1 (en) 2000-02-15 2001-09-05 Johannes Petrus Schouten Multiplex ligation dependent amplification assay
AU2001227679A1 (en) 2000-02-25 2001-09-03 General Atomics Mutant nucleic binding enzymes and use thereof in diagnostic, detection and purification methods
US6833450B1 (en) 2000-03-17 2004-12-21 Affymetrix, Inc. Phosphite ester oxidation in nucleic acid array preparation
US6365355B1 (en) 2000-03-28 2002-04-02 The Regents Of The University Of California Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches
US6969587B2 (en) 2000-04-04 2005-11-29 Taylor Paul D Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography
US20020025561A1 (en) 2000-04-17 2002-02-28 Hodgson Clague Pitman Vectors for gene-self-assembly
WO2001081568A1 (en) 2000-04-21 2001-11-01 Genencor International, Inc. Non-pcr based recombination of nucleic acids
DE10022995C2 (de) 2000-05-11 2003-11-27 Wavelight Laser Technologie Ag Vorrichtung für die photorefraktive Hornhautchirurgie
US6479262B1 (en) 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
WO2001090340A2 (en) 2000-05-21 2001-11-29 University Of North Carolina At Chapel Hill Assembly of large viral genomes and chromosomes from subclones
AU2001275091A1 (en) 2000-06-02 2001-12-17 Blue Heron Biotechnology, Inc. Methods for improving the sequence fidelity of synthetic double-stranded oligonucleotides
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
CN1232343C (zh) 2000-07-03 2005-12-21 艾克索特罗恩公司 利用光生反应物进行化学反应的设备及方法
US20030118486A1 (en) 2000-07-03 2003-06-26 Xeotron Corporation Fluidic methods and devices for parallel chemical reactions
GB0016472D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method and apparatus
WO2002004597A2 (en) 2000-07-07 2002-01-17 Nimblegen Systems, Inc. Method and apparatus for synthesis of arrays of dna probes
AU2001277014A1 (en) 2000-07-21 2002-02-05 Trustees Of Boston University Modular vector systems
GB0018876D0 (en) 2000-08-01 2000-09-20 Applied Research Systems Method of producing polypeptides
DK1311661T3 (da) 2000-08-14 2012-11-26 Us Gov Health & Human Serv Forøget homolog rekombination medieret ved lambda-rekombinationsproteiner
US20020132308A1 (en) 2000-08-24 2002-09-19 Mpep @ Page 300-M Novel constructs and their use in metabolic pathway engineering
US6610499B1 (en) 2000-08-31 2003-08-26 The Regents Of The University Of California Capillary array and related methods
US6966945B1 (en) 2000-09-20 2005-11-22 Goodrich Corporation Inorganic matrix compositions, composites and process of making the same
US20020037359A1 (en) 2000-09-25 2002-03-28 Mutz Mitchell W. Focused acoustic energy in the preparation of peptide arrays
US6666541B2 (en) 2000-09-25 2003-12-23 Picoliter Inc. Acoustic ejection of fluids from a plurality of reservoirs
US6658802B2 (en) 2000-10-24 2003-12-09 Northwest Rubber Extruders, Inc. Seal for fixed window of motor vehicle with curved concave corners
EP1205548A1 (en) 2000-11-09 2002-05-15 Kabushiki Kaisha Toyota Chuo Kenkyusho Methods and apparatus for successively ligating double-stranded DNA molecules on a solid phase
US6709861B2 (en) 2000-11-17 2004-03-23 Lucigen Corp. Cloning vectors and vector components
AU2002227156A1 (en) 2000-12-01 2002-06-11 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US6596239B2 (en) 2000-12-12 2003-07-22 Edc Biosystems, Inc. Acoustically mediated fluid transfer methods and uses thereof
US6660475B2 (en) 2000-12-15 2003-12-09 New England Biolabs, Inc. Use of site-specific nicking endonucleases to create single-stranded regions and applications thereof
US20020133359A1 (en) 2000-12-26 2002-09-19 Appareon System, method and article of manufacture for country and regional treatment in a supply chain system
EP1385950B1 (en) 2001-01-19 2008-07-02 Centocor, Inc. Computer-directed assembly of a polynucleotide encoding a target polypeptide
US6514704B2 (en) 2001-02-01 2003-02-04 Xerox Corporation Quality control mechanism and process for a biofluid multi-ejector system
WO2002077286A1 (en) 2001-02-21 2002-10-03 Gene Check, Inc. Mutation detection using muts and reca
ATE312841T1 (de) 2001-03-08 2005-12-15 Applera Corp Verfahren zum entschützen von oligonukleotide
US7211654B2 (en) 2001-03-14 2007-05-01 Regents Of The University Of Michigan Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports
US20030082630A1 (en) 2001-04-26 2003-05-01 Maxygen, Inc. Combinatorial libraries of monomer domains
US6951720B2 (en) 2001-05-10 2005-10-04 San Diego State University Foundation Use of phosphorothiolate polynucleotides in ligating nucleic acids
CA2447240C (en) 2001-05-18 2013-02-19 Wisconsin Alumni Research Foundation Method for the synthesis of dna sequences
AU2002314997A1 (en) 2001-06-05 2002-12-16 Gorilla Genomics, Inc. Methods for low background cloning of dna using long oligonucleotides
WO2002101004A2 (en) 2001-06-08 2002-12-19 Shanghai Mendel Dna Center Co., Ltd Low-temperature cycle extension of dna with high priming specificity
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7179423B2 (en) 2001-06-20 2007-02-20 Cytonome, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
US6416164B1 (en) 2001-07-20 2002-07-09 Picoliter Inc. Acoustic ejection of fluids using large F-number focusing elements
US20050130140A1 (en) 2001-07-23 2005-06-16 Bovenberg Roelof A.L. Process for preparing variant polynucleotides
US6734436B2 (en) 2001-08-07 2004-05-11 Sri International Optical microfluidic devices and methods
WO2003033718A1 (en) 2001-10-17 2003-04-24 Global Genomics Ab Synthesis of oligonucleotides on solid support and assembly into doublestranded polynucleotides
US20030087298A1 (en) 2001-11-02 2003-05-08 Roland Green Detection of hybridization on oligonucleotide microarray through covalently labeling microarray probe
EP1314783B1 (de) * 2001-11-22 2008-11-19 Sloning BioTechnology GmbH Nukleinsäure-Linker und deren Verwendung in der Gensynthese
US20030099952A1 (en) 2001-11-26 2003-05-29 Roland Green Microarrays with visible pattern detection
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
AU2002357249A1 (en) 2001-12-13 2003-07-09 Blue Heron Biotechnology, Inc. Methods for removal of double-stranded oligonucleotides containing sequence errors using mismatch recognition proteins
AU2003207448A1 (en) 2002-01-04 2003-07-24 Board Of Regents, The University Of Texas System Proofreading, error deletion, and ligation method for synthesis of high-fidelity polynucleotide sequences
US6897025B2 (en) 2002-01-07 2005-05-24 Perlegen Sciences, Inc. Genetic analysis systems and methods
CA2473434A1 (en) 2002-01-14 2003-07-24 Diversa Corporation Methods for making polynucleotides and purifying double-stranded polynucleotides
US20030219781A1 (en) 2002-01-14 2003-11-27 Diversa Corporation Compositions and methods for making polynucleotides by iterative assembly of codon building blocks
US20030215837A1 (en) 2002-01-14 2003-11-20 Diversa Corporation Methods for purifying double-stranded nucleic acids lacking base pair mismatches or nucleotide gaps
US20040096826A1 (en) 2002-01-30 2004-05-20 Evans Glen A. Methods for creating recombination products between nucleotide sequences
US20040126757A1 (en) 2002-01-31 2004-07-01 Francesco Cerrina Method and apparatus for synthesis of arrays of DNA probes
US7037659B2 (en) 2002-01-31 2006-05-02 Nimblegen Systems Inc. Apparatus for constructing DNA probes having a prismatic and kaleidoscopic light homogenizer
US7422851B2 (en) 2002-01-31 2008-09-09 Nimblegen Systems, Inc. Correction for illumination non-uniformity during the synthesis of arrays of oligomers
US7157229B2 (en) 2002-01-31 2007-01-02 Nimblegen Systems, Inc. Prepatterned substrate for optical synthesis of DNA probes
US7083975B2 (en) 2002-02-01 2006-08-01 Roland Green Microarray synthesis instrument and method
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
IL163788A0 (en) 2002-02-28 2005-12-18 Wisconsin Alumni Res Found Method of error reduction in nucleic acid populations
EP1488006B1 (en) 2002-03-20 2008-05-28 InnovativeBio.Biz Microcapsules with controlable permeability encapsulating a nucleic acid amplification reaction mixture and their use as reaction compartments for parallels reactions
US20030182172A1 (en) 2002-03-25 2003-09-25 Claggett Stuart Lee System and method to build project management processes
JP2005521419A (ja) 2002-04-01 2005-07-21 ブルー ヘロン バイオテクノロジー インコーポレイテッド ポリヌクレオチド作製のための固相方法
US7482119B2 (en) 2002-04-01 2009-01-27 Blue Heron Biotechnology, Inc. Solid phase methods for polynucleotide production
US7419784B2 (en) 2002-04-02 2008-09-02 Dubrow Robert S Methods, systems and apparatus for separation and isolation of one or more sample components of a sample biological material
DK1501947T3 (da) 2002-04-22 2008-11-17 Genencor Int Fremgangsmåde til at skabe et bibliotek af bakterielle kloner med varierende niveauer af genekspression
US20040171047A1 (en) 2002-05-22 2004-09-02 Dahl Gary A. Target-dependent transcription
AU2003240795A1 (en) 2002-05-24 2003-12-12 Invitrogen Corporation Nested pcr employing degradable primers
EP1546378B1 (en) 2002-05-24 2011-06-22 Roche NimbleGen, Inc. Microarrays and method for running hybridization reaction for multiple samples on a single microarray
US6932097B2 (en) 2002-06-18 2005-08-23 Picoliter Inc. Acoustic control of the composition and/or volume of fluid in a reservoir
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
US20040101444A1 (en) 2002-07-15 2004-05-27 Xeotron Corporation Apparatus and method for fluid delivery to a hybridization station
GB0221053D0 (en) 2002-09-11 2002-10-23 Medical Res Council Single-molecule in vitro evolution
US7563600B2 (en) * 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
JP2006517090A (ja) 2002-09-26 2006-07-20 コーサン バイオサイエンシーズ， インコーポレイテッド 合成遺伝子
US7498176B2 (en) 2002-09-27 2009-03-03 Roche Nimblegen, Inc. Microarray with hydrophobic barriers
US7482170B2 (en) 2002-09-30 2009-01-27 Roche Nimblegen, Inc. Parallel loading of arrays
US20040110212A1 (en) 2002-09-30 2004-06-10 Mccormick Mark Microarrays with visual alignment marks
WO2004031351A2 (en) 2002-10-01 2004-04-15 Nimblegen Systems, Inc. Microarrays having multiple oligonucleotides in single array features
WO2004034028A2 (en) 2002-10-09 2004-04-22 The Board Of Trustees Of The University Of Illinois Microfluidic systems and components
ES2310197T3 (es) * 2002-10-18 2009-01-01 Sloning Biotechnology Gmbh Metodo para la preparacion de moleculas de acido nucleico.
US7267984B2 (en) 2002-10-31 2007-09-11 Rice University Recombination assembly of large DNA fragments
US7932025B2 (en) 2002-12-10 2011-04-26 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules with error control
US7879580B2 (en) * 2002-12-10 2011-02-01 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules
CA2513899C (en) 2003-01-29 2013-03-26 454 Corporation Methods of amplifying and sequencing nucleic acids
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
EP1613776A1 (en) 2003-04-02 2006-01-11 Blue Heron Biotechnology, Inc. Error reduction in automated gene synthesis
WO2004113534A1 (en) 2003-05-22 2004-12-29 University Of California Method for producing a synthetic gene or other dna sequence
US20040241655A1 (en) 2003-05-29 2004-12-02 Yuchi Hwang Conditional touchdown multiplex polymerase chain reaction
US8133670B2 (en) 2003-06-13 2012-03-13 Cold Spring Harbor Laboratory Method for making populations of defined nucleic acid molecules
CN1833034B (zh) 2003-06-20 2014-04-16 埃克斯魁恩公司 用于分析核酸混合物的探针、文库和试剂盒及其构建方法
US20050069928A1 (en) 2003-08-04 2005-03-31 Blue Heron Biotechnology, Inc. Methods for synthesis of defined polynucleotides
WO2005014796A2 (en) 2003-08-08 2005-02-17 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
US7198900B2 (en) 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7582858B2 (en) 2004-01-23 2009-09-01 Sri International Apparatus and method of moving micro-droplets using laser-induced thermal gradients
ES2353302T3 (es) 2004-01-23 2011-03-01 Sloning Biotechnology Gmbh Producción enzimática de novo de moléculas de ácido nucleico.
AU2005222788A1 (en) * 2004-02-27 2005-09-29 President And Fellows Of Harvard College Polynucleotide synthesis
US7432055B2 (en) 2004-03-05 2008-10-07 Uchicago Argonne Llc Dual phase multiplex polymerase chain reaction
US20050208503A1 (en) 2004-03-16 2005-09-22 Handy Yowanto Chemical ligation of nucleic acids
US20050227316A1 (en) 2004-04-07 2005-10-13 Kosan Biosciences, Inc. Synthetic genes
US8825411B2 (en) 2004-05-04 2014-09-02 Dna Twopointo, Inc. Design, synthesis and assembly of synthetic nucleic acids
JP2005319407A (ja) 2004-05-10 2005-11-17 Hitachi Ltd 圧電デバイスを用いた機器
AU2005254972B2 (en) 2004-06-09 2010-11-04 Wisconsin Alumni Research Foundation Rapid production of oligonucleotides
US20060008833A1 (en) 2004-07-12 2006-01-12 Jacobson Joseph M Method for long, error-reduced DNA synthesis
AU2005279931B2 (en) 2004-08-27 2011-02-10 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations
JP2008512129A (ja) 2004-09-10 2008-04-24 セクエノム，インコーポレイティド 核酸の広範囲配列分析法
US20060194214A1 (en) * 2005-02-28 2006-08-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
US20070122817A1 (en) * 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
JP2008523786A (ja) * 2004-10-18 2008-07-10 コドン デバイシズ インコーポレイテッド 高忠実度合成ポリヌクレオチドのアセンブリ方法
WO2006049843A1 (en) 2004-11-01 2006-05-11 Parallele Bioscience, Inc. Multiplex polynucleotide synthesis
US20060160138A1 (en) 2005-01-13 2006-07-20 George Church Compositions and methods for protein design
EP2230316A1 (en) 2005-02-01 2010-09-22 AB Advanced Genetic Analysis Corporation Nucleic acid sequencing by performing successive cycles of duplex extension
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
WO2006086209A2 (en) 2005-02-10 2006-08-17 Compass Genetics, Llc Genetic analysis by sequence-specific sorting
CN101287845B (zh) 2005-05-11 2012-07-18 先进液体逻辑公司 用于在多个温度下进行生化或化学反应的方法和设备
WO2006127423A2 (en) 2005-05-18 2006-11-30 Codon Devices, Inc. Methods of producing polynucleotide libraries using scarless ligation
DK2302070T3 (da) 2005-06-23 2012-11-26 Keygene Nv Strategier til identifikation og detektion af polymorfismer med højt gennemløb
WO2007005053A1 (en) 2005-06-30 2007-01-11 Codon Devices, Inc. Hierarchical assembly methods for genome engineering
WO2007008951A1 (en) 2005-07-12 2007-01-18 Codon Devices, Inc. Compositions and methods for design of non-immunogenic proteins
JP2009501522A (ja) 2005-07-12 2009-01-22 コドン デバイシズ インコーポレイテッド 生体触媒工学のための組成物及び方法
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
MY143596A (en) 2005-08-11 2011-06-15 Synthetic Genomics Inc In vitro recombination method
US7303320B1 (en) 2005-12-07 2007-12-04 Ashley David M Pick-up truck cab accessory mounting bracket
WO2007075438A2 (en) 2005-12-15 2007-07-05 Codon Devices, Inc. Polypeptides comprising unnatural amino acids, methods for their production and uses therefor
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
WO2007087347A2 (en) 2006-01-24 2007-08-02 Codon Devices, Inc. Methods, systems, and apparatus for facilitating the design of molecular constructs
CN101460633A (zh) 2006-03-14 2009-06-17 基尼宗生物科学公司 用于核酸测序的方法和装置
WO2007107710A1 (en) 2006-03-17 2007-09-27 Solexa Limited Isothermal methods for creating clonal single molecule arrays
WO2007123742A2 (en) 2006-03-31 2007-11-01 Codon Devices, Inc. Methods and compositions for increasing the fidelity of multiplex nucleic acid assembly
US20070231805A1 (en) 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
EP1842915A1 (en) 2006-04-04 2007-10-10 Libragen Method of in vitro polynucleotide sequences shuffling by recursive circular DNA molecules fragmentation and ligation
WO2007120624A2 (en) 2006-04-10 2007-10-25 Codon Devices, Inc. Concerted nucleic acid assembly reactions
WO2007136834A2 (en) 2006-05-19 2007-11-29 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
WO2007136835A2 (en) 2006-05-19 2007-11-29 Codon Devices, Inc Methods and cells for creating functional diversity and uses thereof
WO2007136833A2 (en) 2006-05-19 2007-11-29 Codon Devices, Inc. Methods and compositions for aptamer production and uses thereof
WO2007136736A2 (en) 2006-05-19 2007-11-29 Codon Devices, Inc. Methods for nucleic acid sorting and synthesis
US20070281309A1 (en) 2006-05-19 2007-12-06 Massachusetts Institute Of Technology Microfluidic-based Gene Synthesis
WO2007136840A2 (en) 2006-05-20 2007-11-29 Codon Devices, Inc. Nucleic acid library design and assembly
WO2008054543A2 (en) 2006-05-20 2008-05-08 Codon Devices, Inc. Oligonucleotides for multiplex nucleic acid assembly
DE602007012045D1 (de) 2006-06-06 2011-03-03 Gen Probe Inc Markierte oligonukleotide und ihre verwendung in nukleinsäureverstärkungsverfahren
WO2008024319A2 (en) 2006-08-20 2008-02-28 Codon Devices, Inc. Microfluidic devices for nucleic acid assembly
DE102006039479A1 (de) 2006-08-23 2008-03-06 Febit Biotech Gmbh Programmierbare Oligonukleotidsynthese
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
EP2078077A2 (en) 2006-10-04 2009-07-15 Codon Devices, Inc Nucleic acid libraries and their design and assembly
US7754429B2 (en) * 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
US20080214408A1 (en) 2006-10-18 2008-09-04 Government Of The Uinted States Of America, Represented By The Secretary Dept. In situ assembly of protein microarrays
WO2008076368A2 (en) 2006-12-13 2008-06-26 Codon Devices, Inc. Fragment-rearranged nucleic acids and uses thereof
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
EP2155868A2 (en) 2007-04-19 2010-02-24 Codon Devices, Inc Engineered nucleases and their uses for nucleic acid assembly
US20090305233A1 (en) 2007-07-03 2009-12-10 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Methods and Reagents for Polynucleotide Assembly
ATE494061T1 (de) 2007-07-10 2011-01-15 Hoffmann La Roche Mikrofluidische vorrichtung, mischverfahren und verwendung der vorrichtung
US20090093378A1 (en) 2007-08-29 2009-04-09 Helen Bignell Method for sequencing a polynucleotide template
KR100957057B1 (ko) 2007-12-03 2010-05-13 래플진(주) 핵산과 신호 프로브의 동시 등온증폭을 이용한 핵산의검출방법
US9409177B2 (en) 2008-03-21 2016-08-09 Lawrence Livermore National Security, Llc Chip-based device for parallel sorting, amplification, detection, and identification of nucleic acid subsequences
CN101577391B (zh) 2008-05-07 2011-03-23 富士康(昆山)电脑接插件有限公司 插头连接器及其制造方法
EP2285974A2 (en) * 2008-05-14 2011-02-23 British Columbia Cancer Agency Branch Gene synthesis by convergent assembly of oligonucleotide subsets
WO2010025310A2 (en) 2008-08-27 2010-03-04 Westend Asset Clearinghouse Company, Llc Methods and devices for high fidelity polynucleotide synthesis
US20100057405A1 (en) 2008-08-28 2010-03-04 Sony Corporation Automated software testing environment
EP3257942B1 (en) 2008-12-18 2020-07-22 ITI Scotland Limited Method for assembly of polynucleic acid sequences
SG174617A1 (en) 2009-04-02 2011-10-28 Fluidigm Corp Multi-primer amplification method for barcoding of target nucleic acids
CN102625850B (zh) 2009-04-03 2014-11-26 蒂莫西·Z·刘 多重核酸检测方法和系统
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
CA2777322A1 (en) 2009-08-12 2011-02-17 President And Fellows Of Harvard College Biodetection methods and compositions
JP2013502914A (ja) 2009-08-31 2013-01-31 ビーエーエスエフ プラント サイエンス カンパニー ゲーエムベーハー 多価不飽和脂肪酸合成の増強を促進する植物の種子特異的遺伝子発現を増強する調節核酸分子
PL389135A1 (pl) 2009-09-28 2011-04-11 Michał Lower Sposób i sonda DNA do otrzymywania endonukleaz restrukcyjnych, zwłaszcza o pożądanej specyficzności sekwencyjnej
WO2011053957A2 (en) 2009-11-02 2011-05-05 Gen9, Inc. Compositions and methods for the regulation of multiple genes of interest in a cell
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
EP3597771A1 (en) 2009-11-25 2020-01-22 Gen9, Inc. Methods and apparatuses for chip-based dna error reduction
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
WO2011085075A2 (en) * 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
US10240194B2 (en) 2010-05-13 2019-03-26 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
US8850219B2 (en) 2010-05-13 2014-09-30 Salesforce.Com, Inc. Secure communications
US9187777B2 (en) 2010-05-28 2015-11-17 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
CN101921840B (zh) 2010-06-30 2014-06-25 深圳华大基因科技有限公司 一种基于dna分子标签技术和dna不完全打断策略的pcr测序方法
EP2633071B1 (en) 2010-10-27 2016-10-12 President and Fellows of Harvard College Compositions of toehold primer duplexes and methods of use
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
CN103502448B (zh) 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
US9809904B2 (en) 2011-04-21 2017-11-07 University Of Washington Through Its Center For Commercialization Methods for retrieval of sequence-verified DNA constructs
EP2530159A1 (en) 2011-06-03 2012-12-05 Sandoz Ag Transcription terminator sequences
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
CN103732744A (zh) 2011-06-15 2014-04-16 Gen9股份有限公司 制备性体外克隆的方法
LT2944693T (lt) 2011-08-26 2019-08-26 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
US20150203839A1 (en) 2011-08-26 2015-07-23 Gen9, Inc. Compositions and Methods for High Fidelity Assembly of Nucleic Acids
US9322023B2 (en) 2011-10-06 2016-04-26 Cornell University Constructs and methods for the assembly of biological pathways
HK1200871A1 (en) 2011-11-16 2015-08-14 Sangamo Therapeutics, Inc. Modified dna-binding proteins and uses thereof
US8939620B2 (en) 2011-12-21 2015-01-27 Kawasaki Jukogyo Kabushiki Kaisha Saddle-riding type automotive vehicle
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US20130281308A1 (en) 2012-04-24 2013-10-24 Gen9, Inc. Methods for sorting nucleic acids and preparative in vitro cloning
IL236303B (en) 2012-06-25 2022-07-01 Gen9 Inc Methods for high-throughput nucleic acid assembly and sequencing
US10047347B2 (en) 2012-11-30 2018-08-14 Ixogen Ltd. Oncolytic adenoviruses with increased proportion of the 156R splicing isoform of the E1B protein
PL3360964T3 (pl) 2012-12-06 2020-03-31 Sigma-Aldrich Co. Llc Modyfikacja i regulacja genomu oparta na crispr
US20140310830A1 (en) 2012-12-12 2014-10-16 Feng Zhang CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes
DK2931891T3 (da) 2012-12-17 2019-08-19 Harvard College Rna-styret modificering af menneskelige genomer
WO2014160059A1 (en) 2013-03-13 2014-10-02 Gen9, Inc. Compositions and methods for synthesis of high fidelity oligonucleotides
WO2014160004A1 (en) 2013-03-13 2014-10-02 Gen9, Inc. Compositions, methods and apparatus for oligonucleotides synthesis
US20140272346A1 (en) 2013-03-15 2014-09-18 Rubicon Technology, Inc. Method of growing aluminum oxide onto substrates by use of an aluminum source in an oxygen environment to create transparent, scratch resistant windows
US10273471B2 (en) 2013-03-15 2019-04-30 Gen 9, Inc. Compositions and methods for multiplex nucleic acids synthesis
CN112301024A (zh) 2013-03-15 2021-02-02 通用医疗公司 使用RNA引导的FokI核酸酶(RFN)提高RNA引导的基因组编辑的特异性
AU2014273082B2 (en) 2013-05-29 2018-11-08 Cellectis A method for producing precise DNA cleavage using Cas9 nickase activity
US10421957B2 (en) 2013-07-29 2019-09-24 Agilent Technologies, Inc. DNA assembly using an RNA-programmable nickase
EP3027771B1 (en) 2013-07-30 2019-01-16 Gen9, Inc. Methods for the production of long length clonal sequence verified nucleic acid constructs
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
WO2015081114A2 (en) 2013-11-27 2015-06-04 Gen9, Inc. Libraries of nucleic acids and methods for making the same
US20170198268A1 (en) 2014-07-09 2017-07-13 Gen9, Inc. Compositions and Methods for Site-Directed DNA Nicking and Cleaving
US20170349925A1 (en) 2014-10-21 2017-12-07 Gingko Bioworks, Inc. Methods for Nucleic Acid Assembly

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