JP6054883B2 - インフルエンザウイルスの再集合 - Google Patents
インフルエンザウイルスの再集合 Download PDFInfo
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- JP6054883B2 JP6054883B2 JP2013548864A JP2013548864A JP6054883B2 JP 6054883 B2 JP6054883 B2 JP 6054883B2 JP 2013548864 A JP2013548864 A JP 2013548864A JP 2013548864 A JP2013548864 A JP 2013548864A JP 6054883 B2 JP6054883 B2 JP 6054883B2
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| US10130697B2 (en) | 2010-03-23 | 2018-11-20 | Wisconsin Alumni Research Foundation (Warf) | Vaccines comprising mutant attenuated influenza viruses |
| ES2655051T3 (es) | 2011-08-26 | 2018-02-16 | Wisconsin Alumni Research Foundation | Virus de la gripe con segmento génico PB2 mutante como vacunas vivas atenuadas |
| US20140274806A1 (en) | 2013-03-15 | 2014-09-18 | Synthetic Genomics Vaccines | Influenza virus reassortment |
| MX2015009330A (es) * | 2013-01-23 | 2016-04-15 | Novartis Ag | Reordenamiento del virus de influenza. |
| CA2914604A1 (en) * | 2013-06-06 | 2014-12-11 | Novartis Ag | Influenza virus reassortment |
| WO2015196150A2 (en) | 2014-06-20 | 2015-12-23 | Wisconsin Alumni Research Foundation (Warf) | Mutations that confer genetic stability to additional genes in influenza viruses |
| RU2605317C1 (ru) * | 2015-06-04 | 2016-12-20 | Федеральное государственное автономное образовательное учреждение высшего образования "Новосибирский национальный исследовательский государственный университет" (Новосибирский государственный университет, НГУ) | АДАПТИРОВАННЫЕ ПАНДЕМИЧЕСКИЕ ШТАММЫ ВИРУСА ГРИППА A/Tomsk/273/2010-MA1(H1N1pdm09), A/Tomsk/273/2010-MA2(H1N1pdm09) И A/Tomsk/273/2010-MA3(H1N1pdm09) ДЛЯ ОЦЕНКИ ДЕЙСТВИЯ ПРОТИВОВИРУСНЫХ ПРЕПАРАТОВ (ВАРИАНТЫ) |
| HK1254344A1 (zh) | 2015-06-26 | 2019-07-19 | Seqirus UK Limited | 抗原匹配的流感疫苗 |
| HUE051783T2 (hu) | 2015-07-07 | 2021-03-29 | Seqirus Uk Ltd | Eljárás immunogén hemagglutinin mennyiségi meghatározására |
| WO2017040203A1 (en) * | 2015-08-28 | 2017-03-09 | Yoshihiro Kawaoka | Generation of infectious influenza viruses from virus-like particles |
| CA3014435C (en) | 2016-02-19 | 2023-04-18 | Wisconsin Alumni Research Foundation (Warf) | Improved influenza b virus replication for vaccine development |
| CN109312310B (zh) | 2016-07-15 | 2022-08-02 | 一般财团法人阪大微生物病研究会 | 重配流感病毒的制备方法 |
| CN111032861B (zh) * | 2017-08-28 | 2023-09-12 | 一般财团法人阪大微生物病研究会 | 重配流感病毒的阶段性制备方法 |
| WO2019084310A1 (en) | 2017-10-25 | 2019-05-02 | Yoshihiro Kawaoka | HAS RECOMBINANT INFLUENZA VIRUSES STABILIZED FOR EGG REPLICATION |
| MX2020004151A (es) | 2017-10-30 | 2020-08-13 | Takeda Pharmaceuticals Co | Detergentes compatibles con el medio ambiente para la inactivacion de virus envueltos en lipidos. |
| US12343390B2 (en) | 2018-08-07 | 2025-07-01 | Wisconsin Alumni Research Foundation (Warf) | Recombinant biologically contained filovirus vaccine |
| JP7655849B2 (ja) | 2018-08-20 | 2025-04-02 | ウィスコンシン アルムニ リサーチ ファンデイション | ヘマグルチニン（ｈａ）タンパク質内の非ドミナントエピトープに対する免疫応答を誘起するためのベクター |
| WO2020163804A1 (en) | 2019-02-08 | 2020-08-13 | Wisconsin Alumni Research Foundation (Warf) | Humanized cell line |
| US11390649B2 (en) | 2019-05-01 | 2022-07-19 | Wisconsin Alumni Research Foundation (Warf) | Influenza virus replication for vaccine development |
| US11807872B2 (en) | 2019-08-27 | 2023-11-07 | Wisconsin Alumni Research Foundation (Warf) | Recombinant influenza viruses with stabilized HA for replication in eggs |
| WO2021150874A1 (en) | 2020-01-24 | 2021-07-29 | Wisconsin Alumni Research Foundation (Warf) | Recombinant influenza viruses with stabilized na |
| US12290562B2 (en) | 2020-03-25 | 2025-05-06 | Wisconsin Alumni Research Foundation (Warf) | Recombinant multivalent influenza viruses |
| JP7779858B2 (ja) * | 2020-04-29 | 2025-12-03 | エスケー バイオサイエンス カンパニー リミテッド | 使い捨て培養工程システムを用いたインフルエンザウイルス生産方法及びインフルエンザウイルス抗原精製条件の迅速確認試験 |
| US11694876B2 (en) | 2021-12-08 | 2023-07-04 | Applied Materials, Inc. | Apparatus and method for delivering a plurality of waveform signals during plasma processing |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HU212924B (en) | 1989-05-25 | 1996-12-30 | Chiron Corp | Adjuvant formulation comprising a submicron oil droplet emulsion |
| WO1995011700A1 (en) | 1993-10-29 | 1995-05-04 | Pharmos Corp. | Submicron emulsions as vaccine adjuvants |
| DE19612967A1 (de) | 1996-04-01 | 1997-10-02 | Behringwerke Ag | Verfahren zur Vermehrung von Influenzaviren in Zellkultur, sowie die durch das Verfahren erhältlichen Influenzaviren |
| DE19612966B4 (de) | 1996-04-01 | 2009-12-10 | Novartis Vaccines And Diagnostics Gmbh & Co. Kg | MDCK-Zellen und Verfahren zur Vermehrung von Influenzaviren |
| TW570803B (en) | 1997-04-09 | 2004-01-11 | Duphar Int Res | Influenza vaccine |
| US6080725A (en) | 1997-05-20 | 2000-06-27 | Galenica Pharmaceuticals, Inc. | Immunostimulating and vaccine compositions employing saponin analog adjuvants and uses thereof |
| ATE384126T1 (de) | 1998-06-12 | 2008-02-15 | Sinai School Medicine | Interferon induzierende genetisch veränderte attenuierte viren |
| TR200200776T2 (tr) | 1999-09-24 | 2002-06-21 | Smithkline Beecham Biologicals S.A. | Burun içi grip virüsü aşısı. |
| JP4698112B2 (ja) | 2000-03-03 | 2011-06-08 | 一般財団法人化学及血清療法研究所 | 無血清培養かつ浮遊培養可能な細胞及び当該細胞を用いたワクチン用ウイルスの製造法 |
| GB0024089D0 (en) | 2000-10-02 | 2000-11-15 | Smithkline Beecham Biolog | Novel compounds |
| ATE503493T1 (de) | 2001-02-23 | 2011-04-15 | Glaxosmithkline Biolog Sa | Influenza vakzinzusammensetzungen zur intradermaler verabreichung |
| JP2004536785A (ja) | 2001-02-23 | 2004-12-09 | グラクソスミスクライン バイオロジカルズ ソシエテ アノニム | 新規なワクチン |
| TWI228420B (en) | 2001-05-30 | 2005-03-01 | Smithkline Beecham Pharma Gmbh | Novel vaccine composition |
| AU2004249133B2 (en) * | 2003-05-28 | 2009-05-28 | Wisconsin Alumni Research Foundation | High titer recombinant influenza viruses for vaccines and gene therapy |
| AU2005230708B2 (en) | 2004-04-05 | 2009-01-15 | Zoetis Services Llc | Microfluidized oil-in-water emulsions and vaccine compositions |
| WO2005113756A1 (en) | 2004-05-14 | 2005-12-01 | Glaxosmithkline Biologicals S.A. | Method |
| NZ551640A (en) | 2004-05-20 | 2010-05-28 | Id Biomedical Corp | Process for the production of an influenza vaccine |
| ATE547511T1 (de) | 2004-12-23 | 2012-03-15 | Medimmune Llc | Nichttumorbildende mdck-zellinie zur vermehrung von viren |
| US7691368B2 (en) | 2005-04-15 | 2010-04-06 | Merial Limited | Vaccine formulations |
| RU2008101367A (ru) | 2005-06-21 | 2009-08-10 | Медиммун, Ллк (Us) | Способы и композиции для экспрессии отрицательной смысловой вирусной рнк в собачьих клетках |
| US8703095B2 (en) | 2005-07-07 | 2014-04-22 | Sanofi Pasteur S.A. | Immuno-adjuvant emulsion |
| CA2627971A1 (en) | 2005-11-01 | 2007-05-10 | Novartis Vaccines And Diagnostics Gmbh & Co Kg. | Cell-derived viral vaccines with low levels of residual cell dna by beta-propiolactone treatment |
| WO2007052061A2 (en) | 2005-11-04 | 2007-05-10 | Novartis Vaccines And Diagnostics Srl | Emulsions with free aqueous-phase surfactant as adjuvants for split influenza vaccines |
| FR2896162B1 (fr) | 2006-01-13 | 2008-02-15 | Sanofi Pasteur Sa | Emulsion huile dans eau thermoreversible |
| WO2007126810A2 (en) * | 2006-03-31 | 2007-11-08 | Warf - Wisconsin Alumni Research Foundation | High titer recombinant influenza viruses for vaccines |
| PT2016194E (pt) | 2006-04-19 | 2015-07-20 | Medimmune Llc | Métodos e composições para expressar arn viral de polaridade negativa em células caninas |
| KR101151202B1 (ko) | 2006-10-12 | 2012-06-11 | 글락소스미스클라인 바이오로지칼즈 에스.에이. | 수중유 에멀젼 애주번트를 포함하는 백신 |
| SG177141A1 (en) | 2006-12-06 | 2012-01-30 | Novartis Ag | Vaccines including antigen from four strains of influenza virus |
| EP2045323A1 (en) | 2007-10-05 | 2009-04-08 | Avir Green Hills Biotechnology Research Development Trade Ag | Linear expression constructs for production of influenza virus particles |
| JP5686741B2 (ja) * | 2008-12-16 | 2015-03-18 | バクスター・インターナショナル・インコーポレイテッドＢａｘｔｅｒ Ｉｎｔｅｒｎａｔｉｏｎａｌ Ｉｎｃｏｒｐ０Ｒａｔｅｄ | インフルエンザワクチンの生成 |
| EP2233152A1 (en) * | 2009-03-24 | 2010-09-29 | Avir Green Hills Biotechnology Research Development Trade Ag | High growth reassortant influenza A virus |
| WO2011012999A1 (en) | 2009-07-31 | 2011-02-03 | Novartis Ag | Reverse genetics systems |
| EP2493912B1 (en) * | 2009-10-26 | 2020-07-29 | Wisconsin Alumni Research Foundation | High titer recombinant influenza viruses with enhanced replication in vero cells |
| ES2655051T3 (es) * | 2011-08-26 | 2018-02-16 | Wisconsin Alumni Research Foundation | Virus de la gripe con segmento génico PB2 mutante como vacunas vivas atenuadas |
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- 2013-03-02 AU AU2013205478A patent/AU2013205478B9/en active Active
- 2013-03-02 CA CA2813723A patent/CA2813723A1/en not_active Abandoned
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| US9422528B2 (en) | 2016-08-23 |
| US20200000909A1 (en) | 2020-01-02 |
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| EP2820126B1 (en) | 2017-05-17 |
| CN103608453B (zh) | 2018-07-24 |
| US20170095549A1 (en) | 2017-04-06 |
| US10245312B2 (en) | 2019-04-02 |
| JP6279503B2 (ja) | 2018-02-14 |
| JP2015119730A (ja) | 2015-07-02 |
| WO2013087945A2 (en) | 2013-06-20 |
| AU2013205478B9 (en) | 2014-11-20 |
| WO2013087945A3 (en) | 2013-11-14 |
| CN103608453A (zh) | 2014-02-26 |
| AU2013205478B2 (en) | 2014-06-26 |
| ES2628301T3 (es) | 2017-08-02 |
| US20140030291A1 (en) | 2014-01-30 |
| CA2813723A1 (en) | 2013-09-02 |
| AU2013205478A1 (en) | 2013-06-06 |
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