JP6032715B2 - サーマルシフトアッセイを使用した、標的タンパク質とのリガンド結合を決定するための方法 - Google Patents

サーマルシフトアッセイを使用した、標的タンパク質とのリガンド結合を決定するための方法 Download PDF

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JP6032715B2
JP6032715B2 JP2014505717A JP2014505717A JP6032715B2 JP 6032715 B2 JP6032715 B2 JP 6032715B2 JP 2014505717 A JP2014505717 A JP 2014505717A JP 2014505717 A JP2014505717 A JP 2014505717A JP 6032715 B2 JP6032715 B2 JP 6032715B2
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protein
target protein
ligand
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JP2014513293A (ja
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ペール ノードルンド，
ペール ノードルンド，
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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    • C07—ORGANIC CHEMISTRY
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    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
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    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/5375—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by changing the physical or chemical properties of the medium or immunochemicals, e.g. temperature, density, pH, partitioning
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    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
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    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9446—Antibacterials
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    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9453—Cardioregulators, e.g. antihypotensives, antiarrhythmics
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
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JP2014505717A 2011-04-18 2012-04-18 サーマルシフトアッセイを使用した、標的タンパク質とのリガンド結合を決定するための方法 Active JP6032715B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1106548.9 2011-04-18
GBGB1106548.9A GB201106548D0 (en) 2011-04-18 2011-04-18 A method for determining ligand binding to a target protein using a thermal shift assahy
PCT/GB2012/050853 WO2012143714A1 (en) 2011-04-18 2012-04-18 Methods for determining ligand binding to a target protein using a thermal shift assay

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JP2014513293A JP2014513293A (ja) 2014-05-29
JP2014513293A5 JP2014513293A5 (cg-RX-API-DMAC7.html) 2015-05-07
JP6032715B2 true JP6032715B2 (ja) 2016-11-30

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US (2) US8969014B2 (cg-RX-API-DMAC7.html)
EP (1) EP2699910B1 (cg-RX-API-DMAC7.html)
JP (1) JP6032715B2 (cg-RX-API-DMAC7.html)
KR (1) KR101940342B1 (cg-RX-API-DMAC7.html)
CN (1) CN103733067B (cg-RX-API-DMAC7.html)
AU (1) AU2012246069B2 (cg-RX-API-DMAC7.html)
CA (1) CA2833076C (cg-RX-API-DMAC7.html)
DK (1) DK2699910T3 (cg-RX-API-DMAC7.html)
ES (1) ES2566637T3 (cg-RX-API-DMAC7.html)
GB (2) GB201106548D0 (cg-RX-API-DMAC7.html)
MY (1) MY171913A (cg-RX-API-DMAC7.html)
SG (1) SG194137A1 (cg-RX-API-DMAC7.html)
WO (1) WO2012143714A1 (cg-RX-API-DMAC7.html)

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GB201106548D0 (en) 2011-04-18 2011-06-01 Evitraproteoma Ab A method for determining ligand binding to a target protein using a thermal shift assahy
US9523693B2 (en) 2011-04-18 2016-12-20 Biotarget Engagement Interest Group Ab Methods for determining ligand binding to a target protein using a thermal shift assay
CN103645324B (zh) * 2013-12-03 2017-02-15 胡朝阳 基于细胞热转移技术的高通量药物靶点筛选方法
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CN112924694A (zh) * 2019-12-06 2021-06-08 中国科学院大连化学物理研究所 一种无歧视的蛋白质热稳定性分析方法
JP7502861B2 (ja) * 2019-12-27 2024-06-19 旭化成株式会社 標的物質と両親媒性高分子との複合体の形成反応の反応温度の評価方法
CN111551749A (zh) * 2020-06-12 2020-08-18 郑州大学 一种鉴定药物靶蛋白的方法
CN114076826A (zh) * 2020-08-20 2022-02-22 中国科学院大连化学物理研究所 一种微球辅助的基于蛋白加热沉淀的药物靶蛋白筛选方法
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CN116577472B (zh) * 2023-07-12 2023-10-31 中国中医科学院中药研究所 植物细胞环境蛋白组学分析方法及应用
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KR101940342B1 (ko) 2019-01-18
KR20140033366A (ko) 2014-03-18
US8969014B2 (en) 2015-03-03
GB201106548D0 (en) 2011-06-01
DK2699910T3 (en) 2016-04-18
CN103733067B (zh) 2016-01-13
CA2833076C (en) 2020-07-14
GB2490404A (en) 2012-10-31
AU2012246069B2 (en) 2016-09-22
CN103733067A (zh) 2014-04-16
HK1192612A1 (zh) 2014-08-22
JP2014513293A (ja) 2014-05-29
AU2012246069A1 (en) 2013-10-31
CA2833076A1 (en) 2012-10-26
ES2566637T3 (es) 2016-04-14
US20140057368A1 (en) 2014-02-27
WO2012143714A1 (en) 2012-10-26
EP2699910A1 (en) 2014-02-26
US9528996B2 (en) 2016-12-27
MY171913A (en) 2019-11-06
GB201206834D0 (en) 2012-05-30
GB2490404B (en) 2013-11-06
EP2699910B1 (en) 2016-03-09
SG194137A1 (en) 2013-11-29
US20150140575A1 (en) 2015-05-21

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