JP2014513293A5 - - Google Patents
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- Publication number
- JP2014513293A5 JP2014513293A5 JP2014505717A JP2014505717A JP2014513293A5 JP 2014513293 A5 JP2014513293 A5 JP 2014513293A5 JP 2014505717 A JP2014505717 A JP 2014505717A JP 2014505717 A JP2014505717 A JP 2014505717A JP 2014513293 A5 JP2014513293 A5 JP 2014513293A5
- Authority
- JP
- Japan
- Prior art keywords
- target protein
- protein
- sample
- ligand
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004169 proteins and genes Human genes 0.000 claims 39
- 108090000623 proteins and genes Proteins 0.000 claims 39
- 238000000034 method Methods 0.000 claims 22
- 239000000523 sample Substances 0.000 claims 16
- 239000003446 ligand Substances 0.000 claims 9
- 210000004027 cell Anatomy 0.000 claims 6
- 238000001914 filtration Methods 0.000 claims 4
- 238000001556 precipitation Methods 0.000 claims 4
- 238000001514 detection method Methods 0.000 claims 3
- 238000004949 mass spectrometry Methods 0.000 claims 3
- 238000002844 melting Methods 0.000 claims 3
- 230000008018 melting Effects 0.000 claims 3
- 239000000047 product Substances 0.000 claims 3
- 239000012805 animal sample Substances 0.000 claims 2
- 238000005119 centrifugation Methods 0.000 claims 2
- 230000009089 cytolysis Effects 0.000 claims 2
- 239000013610 patient sample Substances 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- 238000000926 separation method Methods 0.000 claims 2
- 238000004458 analytical method Methods 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 230000001413 cellular effect Effects 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 239000003814 drug Substances 0.000 claims 1
- 229940079593 drug Drugs 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 239000000706 filtrate Substances 0.000 claims 1
- 108020001507 fusion proteins Proteins 0.000 claims 1
- 102000037865 fusion proteins Human genes 0.000 claims 1
- 238000009630 liquid culture Methods 0.000 claims 1
- 210000002751 lymph Anatomy 0.000 claims 1
- 239000002207 metabolite Substances 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 239000012521 purified sample Substances 0.000 claims 1
- 210000002966 serum Anatomy 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1106548.9 | 2011-04-18 | ||
| GBGB1106548.9A GB201106548D0 (en) | 2011-04-18 | 2011-04-18 | A method for determining ligand binding to a target protein using a thermal shift assahy |
| PCT/GB2012/050853 WO2012143714A1 (en) | 2011-04-18 | 2012-04-18 | Methods for determining ligand binding to a target protein using a thermal shift assay |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014513293A JP2014513293A (ja) | 2014-05-29 |
| JP2014513293A5 true JP2014513293A5 (cg-RX-API-DMAC7.html) | 2015-05-07 |
| JP6032715B2 JP6032715B2 (ja) | 2016-11-30 |
Family
ID=44147179
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014505717A Active JP6032715B2 (ja) | 2011-04-18 | 2012-04-18 | サーマルシフトアッセイを使用した、標的タンパク質とのリガンド結合を決定するための方法 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US8969014B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2699910B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6032715B2 (cg-RX-API-DMAC7.html) |
| KR (1) | KR101940342B1 (cg-RX-API-DMAC7.html) |
| CN (1) | CN103733067B (cg-RX-API-DMAC7.html) |
| AU (1) | AU2012246069B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2833076C (cg-RX-API-DMAC7.html) |
| DK (1) | DK2699910T3 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2566637T3 (cg-RX-API-DMAC7.html) |
| GB (2) | GB201106548D0 (cg-RX-API-DMAC7.html) |
| MY (1) | MY171913A (cg-RX-API-DMAC7.html) |
| SG (1) | SG194137A1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2012143714A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201106548D0 (en) | 2011-04-18 | 2011-06-01 | Evitraproteoma Ab | A method for determining ligand binding to a target protein using a thermal shift assahy |
| US9523693B2 (en) | 2011-04-18 | 2016-12-20 | Biotarget Engagement Interest Group Ab | Methods for determining ligand binding to a target protein using a thermal shift assay |
| CN103645324B (zh) * | 2013-12-03 | 2017-02-15 | 胡朝阳 | 基于细胞热转移技术的高通量药物靶点筛选方法 |
| GB2524519B (en) | 2014-03-25 | 2019-11-06 | Pelago Bioscience AB | Methods for identifying a biomarker indicative of a reduced drug response using a thermal shift assay |
| EP3183580B1 (en) * | 2014-08-21 | 2025-03-26 | Eurofins DiscoverX Corporation | Homogenous thermal shift ligand binding assay |
| EP3254113B1 (en) | 2015-02-05 | 2024-10-09 | Promega Corporation | Luciferase-based thermal shift assays |
| US11024492B2 (en) | 2015-07-07 | 2021-06-01 | Institute For Systems Biology | Method to obtain unbiased identification of interaction of test compounds with the proteome |
| CN105424928B (zh) * | 2015-11-11 | 2017-05-17 | 山西隆克尔生物制药有限公司 | 一种检测猪繁殖与呼吸综合征病毒抗体的免疫层析条及其制备方法和应用 |
| WO2017206041A1 (zh) * | 2016-05-31 | 2017-12-07 | 王帅 | 测定细胞内配体靶蛋白相互作用持久力的方法 |
| US11567074B2 (en) * | 2016-09-26 | 2023-01-31 | University of Pittsburgh—of the Commonwealth System of Higher Education | Methods for identifying proteins that bind ligands |
| KR101862198B1 (ko) * | 2016-09-30 | 2018-05-30 | (주)스파크바이오파마 | 2차원 겔 전기영동에서의 열 안정성 변화-기반 형광 차이를 이용한 표적 단백질의 규명 방법 |
| CN111247431B (zh) * | 2017-08-18 | 2023-07-07 | 新加坡科技研究局 | 鉴定蛋白质相互作用的方法 |
| EP3575416A1 (en) * | 2018-06-01 | 2019-12-04 | InfanDx AG | Detection of target molecules by determining the melting temperature of a recognition module |
| GB201814451D0 (en) * | 2018-09-05 | 2018-10-17 | Valerie Nicholas Carl Kristoffer | Methods |
| CN112924693B (zh) * | 2019-12-06 | 2022-09-27 | 中国科学院大连化学物理研究所 | 一种上清和沉淀协同定量的蛋白质热稳定性分析方法 |
| CN112924694A (zh) * | 2019-12-06 | 2021-06-08 | 中国科学院大连化学物理研究所 | 一种无歧视的蛋白质热稳定性分析方法 |
| JP7502861B2 (ja) * | 2019-12-27 | 2024-06-19 | 旭化成株式会社 | 標的物質と両親媒性高分子との複合体の形成反応の反応温度の評価方法 |
| CN111551749A (zh) * | 2020-06-12 | 2020-08-18 | 郑州大学 | 一种鉴定药物靶蛋白的方法 |
| CN114076826A (zh) * | 2020-08-20 | 2022-02-22 | 中国科学院大连化学物理研究所 | 一种微球辅助的基于蛋白加热沉淀的药物靶蛋白筛选方法 |
| EP4378586A1 (en) | 2022-12-01 | 2024-06-05 | Bayer AG | Integrated system for chemical, biochemical, or biological reactions in a microplate submitted to a temperature gradient |
| WO2024261269A1 (en) | 2023-06-22 | 2024-12-26 | Omicscouts Gmbh | Method for detecting ligand-target interactions using solvent- induced precipitation in intact cells |
| CN116577472B (zh) * | 2023-07-12 | 2023-10-31 | 中国中医科学院中药研究所 | 植物细胞环境蛋白组学分析方法及应用 |
| GB2634751A (en) | 2023-10-18 | 2025-04-23 | Proteinera Holding Ab | Methods for detecting protein cleavage |
| GB2634749A (en) | 2023-10-18 | 2025-04-23 | Proteinera Holding Ab | Methods for detecting apoptosis |
| WO2025247735A1 (en) | 2024-05-29 | 2025-12-04 | Bayer Aktiengesellschaft | Solvent induced precipitation (sip) assays using specific solvent combinations |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4780409A (en) * | 1985-05-02 | 1988-10-25 | Genetic Systems Corporation | Thermally induced phase separation immunoassay |
| KR20000011069A (ko) | 1996-05-09 | 2000-02-25 | 스리-디멘셔널 파마슈티컬스 인코포레이티드 | 리간드 개발 및 다변수 단백질 화학의 최적화를 위한미량이식판 열 이동 분석 및 그 장치 |
| IL136046A0 (en) * | 1997-11-12 | 2001-05-20 | Dimensional Pharm Inc | Methods for classifying proteins |
| US6171850B1 (en) | 1999-03-08 | 2001-01-09 | Caliper Technologies Corp. | Integrated devices and systems for performing temperature controlled reactions and analyses |
| US6376180B1 (en) * | 1999-12-09 | 2002-04-23 | Pharmacia & Upjohn Company | Methods of identifying compounds that bind to target species under isothermal denaturing conditions |
| AU2002315157A1 (en) * | 2001-06-14 | 2003-01-02 | Anadys Pharmaceuticals, Inc. | Methods of screening for ligands of target molecules |
| US20090005257A1 (en) * | 2003-05-14 | 2009-01-01 | Jespers Laurent S | Process for Recovering Polypeptides that Unfold Reversibly from a Polypeptide Repertoire |
| GB0315525D0 (en) | 2003-07-02 | 2003-08-06 | Nordlund Paer | A method of screening |
| JP4695851B2 (ja) | 2003-07-10 | 2011-06-08 | シチズンホールディングス株式会社 | マイクロ化学チップ温度調節装置 |
| EP1796824A4 (en) | 2004-05-02 | 2016-10-19 | Fluidigm Corp | DEVICE FOR THERMAL REACTIONS AND METHOD FOR THEIR USE |
| WO2006110292A2 (en) * | 2005-03-25 | 2006-10-19 | The Regents Of The University Of California | Temperature-triggered immobilization and purification of antibodies |
| LT5504B (lt) * | 2006-08-02 | 2008-06-25 | Biotechnologijos Institutas | Benzimidazo[1,2-c][1,2,3]tiadiazolo sulfonamidai - karboanhidrazių slopikliai ir tarpiniai junginiai jiems gauti |
| US20100256342A1 (en) * | 2008-08-12 | 2010-10-07 | Francis Raymond Salemme | Protein nodes for controlled nanoscale assembly |
| JP2012531582A (ja) * | 2009-06-26 | 2012-12-10 | アトラス・アンティボディーズ・アクチボラゲット | 免疫検出性の改善 |
| US8476081B2 (en) * | 2009-09-21 | 2013-07-02 | Janssen Pharmaceutica Nv | Assay for evaluating affinity and specificity of ligand-albumin binding |
| US9523693B2 (en) | 2011-04-18 | 2016-12-20 | Biotarget Engagement Interest Group Ab | Methods for determining ligand binding to a target protein using a thermal shift assay |
| GB201106548D0 (en) | 2011-04-18 | 2011-06-01 | Evitraproteoma Ab | A method for determining ligand binding to a target protein using a thermal shift assahy |
-
2011
- 2011-04-18 GB GBGB1106548.9A patent/GB201106548D0/en not_active Ceased
-
2012
- 2012-04-18 CA CA2833076A patent/CA2833076C/en active Active
- 2012-04-18 DK DK12717826.7T patent/DK2699910T3/en active
- 2012-04-18 KR KR1020137030379A patent/KR101940342B1/ko active Active
- 2012-04-18 CN CN201280025677.XA patent/CN103733067B/zh active Active
- 2012-04-18 WO PCT/GB2012/050853 patent/WO2012143714A1/en not_active Ceased
- 2012-04-18 GB GB1206834.2A patent/GB2490404B/en active Active
- 2012-04-18 ES ES12717826.7T patent/ES2566637T3/es active Active
- 2012-04-18 EP EP12717826.7A patent/EP2699910B1/en active Active
- 2012-04-18 AU AU2012246069A patent/AU2012246069B2/en active Active
- 2012-04-18 MY MYPI2013003781A patent/MY171913A/en unknown
- 2012-04-18 SG SG2013075072A patent/SG194137A1/en unknown
- 2012-04-18 JP JP2014505717A patent/JP6032715B2/ja active Active
-
2013
- 2013-10-18 US US14/057,920 patent/US8969014B2/en active Active
-
2015
- 2015-01-22 US US14/602,993 patent/US9528996B2/en active Active
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