JP6022556B2 - 配列特異的な操作されたリボヌクレアーゼｈ、および、ｄｎａ−ｒｎａハイブリッド結合タンパク質の配列優先性を決定する方法 - Google Patents

配列特異的な操作されたリボヌクレアーゼｈ、および、ｄｎａ−ｒｎａハイブリッド結合タンパク質の配列優先性を決定する方法 Download PDF

Info

Publication number
JP6022556B2
JP6022556B2 JP2014514834A JP2014514834A JP6022556B2 JP 6022556 B2 JP6022556 B2 JP 6022556B2 JP 2014514834 A JP2014514834 A JP 2014514834A JP 2014514834 A JP2014514834 A JP 2014514834A JP 6022556 B2 JP6022556 B2 JP 6022556B2
Authority
JP
Japan
Prior art keywords
dna
rna
sequence
seq
zfqqr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2014514834A
Other languages
English (en)
Japanese (ja)
Other versions
JP2014516569A5 (enExample
JP2014516569A (ja
Inventor
ヤヌスズ マレク ブジニキ
ヤヌスズ マレク ブジニキ
アグニエスカ スレジ，アガタ
アグニエスカ スレジ，アガタ
イェルジ スコウロネク，クリストフ
イェルジ スコウロネク，クリストフ
ノボトニー，マルチン
Original Assignee
ミエドジナロドウィ インスティタット バイオロジーモレキュラーネイ アイ コモルコウェイ
ミエドジナロドウィ インスティタット バイオロジーモレキュラーネイ アイ コモルコウェイ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PL395180A external-priority patent/PL222513B1/pl
Priority claimed from PL395179A external-priority patent/PL222512B1/pl
Application filed by ミエドジナロドウィ インスティタット バイオロジーモレキュラーネイ アイ コモルコウェイ, ミエドジナロドウィ インスティタット バイオロジーモレキュラーネイ アイ コモルコウェイ filed Critical ミエドジナロドウィ インスティタット バイオロジーモレキュラーネイ アイ コモルコウェイ
Publication of JP2014516569A publication Critical patent/JP2014516569A/ja
Publication of JP2014516569A5 publication Critical patent/JP2014516569A5/ja
Application granted granted Critical
Publication of JP6022556B2 publication Critical patent/JP6022556B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/26—Endoribonucleases producing 5'-phosphomonoesters (3.1.26)
    • C12Y301/26004—Ribonuclease H (3.1.26.4)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2014514834A 2011-06-08 2012-06-07 配列特異的な操作されたリボヌクレアーゼｈ、および、ｄｎａ−ｒｎａハイブリッド結合タンパク質の配列優先性を決定する方法 Expired - Fee Related JP6022556B2 (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201161494481P 2011-06-08 2011-06-08
US201161494494P 2011-06-08 2011-06-08
PL395180A PL222513B1 (pl) 2011-06-08 2011-06-08 Sposób określania sekwencji preferowanych przez białko lub jego domeny wiążące specyficznie sekwencje w hybrydzie DNA-RNA
PL395179A PL222512B1 (pl) 2011-06-08 2011-06-08 Endorybonukleaza przecinająca nić RNA w hybrydach DNA-RNA
PLP.395179 2011-06-08
US61/494,494 2011-06-08
PLP.395180 2011-06-08
US61/494,481 2011-06-08
PCT/PL2012/050019 WO2012169916A2 (en) 2011-06-08 2012-06-07 Sequence-specific engineered ribonuclease h and the method for determining the sequence preference of dna-rna hybrid binding proteins

Publications (3)

Publication Number Publication Date
JP2014516569A JP2014516569A (ja) 2014-07-17
JP2014516569A5 JP2014516569A5 (enExample) 2014-10-16
JP6022556B2 true JP6022556B2 (ja) 2016-11-09

Family

ID=47296662

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014514834A Expired - Fee Related JP6022556B2 (ja) 2011-06-08 2012-06-07 配列特異的な操作されたリボヌクレアーゼｈ、および、ｄｎａ−ｒｎａハイブリッド結合タンパク質の配列優先性を決定する方法

Country Status (10)

Country Link
US (1) US9353358B2 (enExample)
EP (2) EP2718430B1 (enExample)
JP (1) JP6022556B2 (enExample)
KR (1) KR20140066977A (enExample)
CN (1) CN103764820A (enExample)
AU (1) AU2012267270B2 (enExample)
CA (1) CA2837503C (enExample)
DK (1) DK2718430T3 (enExample)
ES (1) ES2557633T3 (enExample)
WO (1) WO2012169916A2 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3328353B1 (en) 2015-07-27 2024-04-10 Mary Kay, Inc. Topical skin formulations
CN108314736B (zh) * 2017-01-18 2021-08-31 李燕强 一种促进rna降解的方法
CN110846724B (zh) * 2019-12-03 2023-04-28 江西海普洛斯医学检验实验室有限公司 构建mRNA链特异文库的方法及试剂盒
US12416044B2 (en) * 2022-03-21 2025-09-16 Abclonal Science, Inc. T4 DNA ligase variants with increased ligation efficiency

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5459392A (en) 1977-10-14 1979-05-12 Rikagaku Kenkyusho Novel nuclease and its preparation
US5270163A (en) * 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
AU2002217830A1 (en) * 2000-11-15 2002-05-27 Isis Pharmaceuticals, Inc. Human rnase h1 mutants
WO2003087341A2 (en) 2002-01-23 2003-10-23 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
US20060057590A1 (en) 2004-09-14 2006-03-16 Azeddine Si-Ammour RNA probes
US20100055793A1 (en) 2005-07-25 2010-03-04 Johns Hopkins University Site-specific modification of the human genome using custom-designed zinc finger nucleases
US7804803B2 (en) 2005-07-25 2010-09-28 Honeywell International Inc. Neighbor based TDMA slot assignment
WO2009126632A1 (en) * 2008-04-08 2009-10-15 Archemix Corp. Compositions and methods for the use of mutant t3 rna polymerases in the synthesis of modified nucleic acid transcripts
WO2009146179A1 (en) 2008-04-15 2009-12-03 University Of Iowa Research Foundation Zinc finger nuclease for the cftr gene and methods of use thereof
KR20100080068A (ko) 2008-12-31 2010-07-08 주식회사 툴젠 신규한 징크 핑거 뉴클레아제 및 이의 용도
WO2011022427A1 (en) * 2009-08-18 2011-02-24 Archemix Corp. Aptamers to tissue factor pathway inhibitor and their use as bleeding disorder therapeutics

Also Published As

Publication number Publication date
CA2837503C (en) 2018-01-02
EP2718430B1 (en) 2015-12-09
AU2012267270A1 (en) 2013-12-12
US9353358B2 (en) 2016-05-31
DK2718430T3 (en) 2016-02-08
ES2557633T3 (es) 2016-01-27
EP2857506A3 (en) 2015-04-29
US20140094385A1 (en) 2014-04-03
WO2012169916A3 (en) 2013-03-07
EP2857506A2 (en) 2015-04-08
WO2012169916A2 (en) 2012-12-13
EP2718430A2 (en) 2014-04-16
CN103764820A (zh) 2014-04-30
AU2012267270B2 (en) 2015-06-11
KR20140066977A (ko) 2014-06-03
JP2014516569A (ja) 2014-07-17
CA2837503A1 (en) 2012-12-13

Similar Documents

Publication Publication Date Title
CN102796728B (zh) 用于通过转座酶的dna片段化和标记的方法和组合物
US20230407341A1 (en) Using Truncated Guide RNAs (tru-gRNAs) to Increase Specificity for RNA-Guided Genome Editing
US10100348B2 (en) Immobilized transposase complexes for DNA fragmentation and tagging
JP6075892B2 (ja) 二本鎖ｒｎａエンドリボヌクレアーゼ
JP2022106883A (ja) Ｃａｓ９ターゲッティングをガイドする配列に関する方法および組成物
ES2705756T3 (es) Secuencias de reconocimiento para meganucleasas derivadas de I-Crel y sus usos
CN118726313A (zh) 化脓链球菌cas9突变基因和由其编码的多肽
JP2017500035A (ja) 遺伝子編集用のｃａｓ多様体
JP2015503535A (ja) 改変されたｃａｓｃａｄｅリボ核タンパク質およびそれらの用途
JP6022556B2 (ja) 配列特異的な操作されたリボヌクレアーゼｈ、および、ｄｎａ−ｒｎａハイブリッド結合タンパク質の配列優先性を決定する方法
Nguyen et al. Activity, stability, and structure of metagenome‐derived LC11‐RNase H1, a homolog of Sulfolobus tokodaii RNase H1
CN110291208B (zh) 单链结合蛋白
PL222512B1 (pl) Endorybonukleaza przecinająca nić RNA w hybrydach DNA-RNA
Setayesh et al. Cloning, molecular characterization and expression of a DNA-ligase from a new bacteriophage: Phax1
PL222513B1 (pl) Sposób określania sekwencji preferowanych przez białko lub jego domeny wiążące specyficznie sekwencje w hybrydzie DNA-RNA

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140205

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140829

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20141113

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20151118

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160218

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20160601

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160823

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20160914

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20161005

R150 Certificate of patent or registration of utility model

Ref document number: 6022556

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

LAPS Cancellation because of no payment of annual fees