CN103764820A - 序列特异性工程改造的核糖核酸酶h和用于确定dna-rna杂交物结合蛋白序列偏好的方法 - Google Patents

序列特异性工程改造的核糖核酸酶h和用于确定dna-rna杂交物结合蛋白序列偏好的方法 Download PDF

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Publication number
CN103764820A
CN103764820A CN201280027647.2A CN201280027647A CN103764820A CN 103764820 A CN103764820 A CN 103764820A CN 201280027647 A CN201280027647 A CN 201280027647A CN 103764820 A CN103764820 A CN 103764820A
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China
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rna
dna
sequence
seq
hybrid
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CN201280027647.2A
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English (en)
Chinese (zh)
Inventor
J.M.布尼基
A.A.苏勒
K.J.斯科罗内克
M.诺沃特尼
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MIEDZYNARODOWY INST BIOLOG MOLEKULARNEJ I KOMORKOWEJ
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MIEDZYNARODOWY INST BIOLOG MOLEKULARNEJ I KOMORKOWEJ
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Priority claimed from PL395179A external-priority patent/PL222512B1/pl
Priority claimed from PL395180A external-priority patent/PL222513B1/pl
Application filed by MIEDZYNARODOWY INST BIOLOG MOLEKULARNEJ I KOMORKOWEJ filed Critical MIEDZYNARODOWY INST BIOLOG MOLEKULARNEJ I KOMORKOWEJ
Publication of CN103764820A publication Critical patent/CN103764820A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/26—Endoribonucleases producing 5'-phosphomonoesters (3.1.26)
    • C12Y301/26004—Ribonuclease H (3.1.26.4)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201280027647.2A 2011-06-08 2012-06-07 序列特异性工程改造的核糖核酸酶h和用于确定dna-rna杂交物结合蛋白序列偏好的方法 Pending CN103764820A (zh)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201161494481P 2011-06-08 2011-06-08
US201161494494P 2011-06-08 2011-06-08
US61/494494 2011-06-08
US61/494481 2011-06-08
PLP.395179 2011-06-08
PL395179A PL222512B1 (pl) 2011-06-08 2011-06-08 Endorybonukleaza przecinająca nić RNA w hybrydach DNA-RNA
PL395180A PL222513B1 (pl) 2011-06-08 2011-06-08 Sposób określania sekwencji preferowanych przez białko lub jego domeny wiążące specyficznie sekwencje w hybrydzie DNA-RNA
PLP.395180 2011-06-08
PCT/PL2012/050019 WO2012169916A2 (en) 2011-06-08 2012-06-07 Sequence-specific engineered ribonuclease h and the method for determining the sequence preference of dna-rna hybrid binding proteins

Publications (1)

Publication Number Publication Date
CN103764820A true CN103764820A (zh) 2014-04-30

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CN201280027647.2A Pending CN103764820A (zh) 2011-06-08 2012-06-07 序列特异性工程改造的核糖核酸酶h和用于确定dna-rna杂交物结合蛋白序列偏好的方法

Country Status (10)

Country Link
US (1) US9353358B2 (enExample)
EP (2) EP2857506A3 (enExample)
JP (1) JP6022556B2 (enExample)
KR (1) KR20140066977A (enExample)
CN (1) CN103764820A (enExample)
AU (1) AU2012267270B2 (enExample)
CA (1) CA2837503C (enExample)
DK (1) DK2718430T3 (enExample)
ES (1) ES2557633T3 (enExample)
WO (1) WO2012169916A2 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108314736A (zh) * 2017-01-18 2018-07-24 李燕强 一种促进rna降解的方法
CN110846724A (zh) * 2019-12-03 2020-02-28 江西海普洛斯医学检验实验室有限公司 构建mRNA链特异文库的方法及试剂盒
CN114934026A (zh) * 2022-03-21 2022-08-23 武汉爱博泰克生物科技有限公司 具有增加的连接效率的t4 dna连接酶变体
US11577099B2 (en) 2015-07-27 2023-02-14 Mary Kay Inc. Topical skin formulations

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011022427A1 (en) * 2009-08-18 2011-02-24 Archemix Corp. Aptamers to tissue factor pathway inhibitor and their use as bleeding disorder therapeutics

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JPS5459392A (en) 1977-10-14 1979-05-12 Rikagaku Kenkyusho Novel nuclease and its preparation
US5270163A (en) * 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US6855530B2 (en) * 2000-11-15 2005-02-15 Isis Pharmaceuticals, Inc. Human RNase H1 mutants
WO2003087341A2 (en) 2002-01-23 2003-10-23 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
US20060057590A1 (en) 2004-09-14 2006-03-16 Azeddine Si-Ammour RNA probes
US20100055793A1 (en) 2005-07-25 2010-03-04 Johns Hopkins University Site-specific modification of the human genome using custom-designed zinc finger nucleases
US7804803B2 (en) 2005-07-25 2010-09-28 Honeywell International Inc. Neighbor based TDMA slot assignment
WO2009126632A1 (en) * 2008-04-08 2009-10-15 Archemix Corp. Compositions and methods for the use of mutant t3 rna polymerases in the synthesis of modified nucleic acid transcripts
WO2009146179A1 (en) 2008-04-15 2009-12-03 University Of Iowa Research Foundation Zinc finger nuclease for the cftr gene and methods of use thereof
KR20100080068A (ko) 2008-12-31 2010-07-08 주식회사 툴젠 신규한 징크 핑거 뉴클레아제 및 이의 용도

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011022427A1 (en) * 2009-08-18 2011-02-24 Archemix Corp. Aptamers to tissue factor pathway inhibitor and their use as bleeding disorder therapeutics

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
AGATA A. SULEJ等: "Sequence-specific cleavage of the RNA strand in DNA–RNA hybrids by the fusion of ribonuclease H with a zinc finger", 《NUCLEIC ACIDS RESEARCH》 *
DURAI S等: "Zinc finger nucleases: custom-designed molecular scissors for genome engineering of plant and mammalian cells", 《NUCLEIC ACIDS RESEARCH》 *
MARCIN NOWOTNY等: "Specific recognition of RNA/DNA hybrid and enhancement of human RNase H1 activity by HBD", 《THE EMBO JOURNAL》 *
T. KEITH BLACKWELL等: "Differences and Similarities in DNA-Binding Preferences of MyoD and E2A Protein Complexes Revealed by Binding Site Selection", 《SCIENCE》 *
TAKAMI,H.等: "BAB04582.1", 《GENBANK》 *
YANG-GYUN KIM等: "Site-specific cleavage of DNA–RNA hybrids by zinc finger/FokI cleavage domain fusions", 《GENE》 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11577099B2 (en) 2015-07-27 2023-02-14 Mary Kay Inc. Topical skin formulations
US11911635B2 (en) 2015-07-27 2024-02-27 Mary Kay Inc. Topical skin formulations
US12161891B2 (en) 2015-07-27 2024-12-10 Mary Kay Inc. Topical skin formulations
CN108314736A (zh) * 2017-01-18 2018-07-24 李燕强 一种促进rna降解的方法
CN108314736B (zh) * 2017-01-18 2021-08-31 李燕强 一种促进rna降解的方法
CN110846724A (zh) * 2019-12-03 2020-02-28 江西海普洛斯医学检验实验室有限公司 构建mRNA链特异文库的方法及试剂盒
CN114934026A (zh) * 2022-03-21 2022-08-23 武汉爱博泰克生物科技有限公司 具有增加的连接效率的t4 dna连接酶变体
CN114934026B (zh) * 2022-03-21 2023-09-26 武汉爱博泰克生物科技有限公司 具有增加的连接效率的t4 dna连接酶变体

Also Published As

Publication number Publication date
WO2012169916A3 (en) 2013-03-07
CA2837503A1 (en) 2012-12-13
JP6022556B2 (ja) 2016-11-09
ES2557633T3 (es) 2016-01-27
EP2718430B1 (en) 2015-12-09
EP2857506A3 (en) 2015-04-29
US20140094385A1 (en) 2014-04-03
KR20140066977A (ko) 2014-06-03
WO2012169916A2 (en) 2012-12-13
US9353358B2 (en) 2016-05-31
AU2012267270B2 (en) 2015-06-11
EP2718430A2 (en) 2014-04-16
AU2012267270A1 (en) 2013-12-12
JP2014516569A (ja) 2014-07-17
EP2857506A2 (en) 2015-04-08
CA2837503C (en) 2018-01-02
DK2718430T3 (en) 2016-02-08

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Application publication date: 20140430