JP5973563B2 - 相同組み換えによる酵母形質転換体の形質転換用および選択用のカセットならびに方法 - Google Patents

相同組み換えによる酵母形質転換体の形質転換用および選択用のカセットならびに方法 Download PDF

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JP5973563B2
JP5973563B2 JP2014516449A JP2014516449A JP5973563B2 JP 5973563 B2 JP5973563 B2 JP 5973563B2 JP 2014516449 A JP2014516449 A JP 2014516449A JP 2014516449 A JP2014516449 A JP 2014516449A JP 5973563 B2 JP5973563 B2 JP 5973563B2
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vector
promoter
homologous recombination
selection gene
nucleic acid
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JP2014519840A (ja
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クリステル ペリン−イースト
クリステル ペリン−イースト
パブロ グルシャンコフ
パブロ グルシャンコフ
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アミカナ， バイオロジクス
アミカナ， バイオロジクス
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2014516449A 2011-06-20 2011-06-20 相同組み換えによる酵母形質転換体の形質転換用および選択用のカセットならびに方法 Expired - Fee Related JP5973563B2 (ja)

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PCT/IB2011/002254 WO2012176016A1 (en) 2011-06-20 2011-06-20 Cassettes and methods for transforming and selecting yeast transformants by homologous recombination

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JP2014519840A JP2014519840A (ja) 2014-08-21
JP5973563B2 true JP5973563B2 (ja) 2016-08-23

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US (1) US20140120624A1 (https=)
EP (1) EP2721159A1 (https=)
JP (1) JP5973563B2 (https=)
CN (1) CN103917648B (https=)
BR (1) BR112013033177A8 (https=)
WO (1) WO2012176016A1 (https=)

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EP3088535A1 (en) 2015-04-29 2016-11-02 Amikana. Biologics Faculté de Médecine de la Timone Methods for identifying antiretroviral compounds targeting the reverse transcriptase of hiv
EP3390640A4 (en) * 2015-12-17 2019-05-22 Evonik Degussa (China) Co., Ltd GENETASSETTE FOR KNOCK-OUT THROUGH HOMOLOGOUS RECOMBINATION IN HEF-CELLS
CN112175984A (zh) * 2020-09-18 2021-01-05 中国科学院深圳先进技术研究院 基于合成基因和酿酒酵母同源重组机制的分子克隆方法

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US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US20030092183A1 (en) * 2001-09-21 2003-05-15 Fisher Katherine E. Rapid creation of gene targeting vectors using homologous recombination in yeast
US9493769B2 (en) 2004-06-02 2016-11-15 Universite D'aix-Marseille Yeast-based assay for measuring the functional activity of an HIV-1 protease in response to an antiviral agent
WO2009139349A1 (ja) * 2008-05-14 2009-11-19 Ｂｉｏ－ｅｎｅｒｇｙ株式会社 酵母細胞に遺伝子を導入する方法およびそのためのベクター

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JP2014519840A (ja) 2014-08-21
BR112013033177A2 (https=) 2012-12-27
CN103917648B (zh) 2016-09-21
US20140120624A1 (en) 2014-05-01
EP2721159A1 (en) 2014-04-23
CN103917648A (zh) 2014-07-09
BR112013033177A8 (pt) 2018-07-10
WO2012176016A1 (en) 2012-12-27
WO2012176016A8 (en) 2013-02-21

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