CN103917648B - 用于通过同源重组转化和选择酵母转化体的盒和方法 - Google Patents

用于通过同源重组转化和选择酵母转化体的盒和方法 Download PDF

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Publication number
CN103917648B
CN103917648B CN201180072966.0A CN201180072966A CN103917648B CN 103917648 B CN103917648 B CN 103917648B CN 201180072966 A CN201180072966 A CN 201180072966A CN 103917648 B CN103917648 B CN 103917648B
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homologous recombination
vector
promoter
selection gene
negative selection
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CN103917648A (zh
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克里斯特勒·佩林-伊斯特
帕布洛·格卢尚科夫
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AMIKANA BIOLOGICS
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AMIKANA BIOLOGICS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201180072966.0A 2011-06-20 2011-06-20 用于通过同源重组转化和选择酵母转化体的盒和方法 Expired - Fee Related CN103917648B (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2011/002254 WO2012176016A1 (en) 2011-06-20 2011-06-20 Cassettes and methods for transforming and selecting yeast transformants by homologous recombination

Publications (2)

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CN103917648A CN103917648A (zh) 2014-07-09
CN103917648B true CN103917648B (zh) 2016-09-21

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CN201180072966.0A Expired - Fee Related CN103917648B (zh) 2011-06-20 2011-06-20 用于通过同源重组转化和选择酵母转化体的盒和方法

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US (1) US20140120624A1 (https=)
EP (1) EP2721159A1 (https=)
JP (1) JP5973563B2 (https=)
CN (1) CN103917648B (https=)
BR (1) BR112013033177A8 (https=)
WO (1) WO2012176016A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112175984A (zh) * 2020-09-18 2021-01-05 中国科学院深圳先进技术研究院 基于合成基因和酿酒酵母同源重组机制的分子克隆方法

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3088535A1 (en) 2015-04-29 2016-11-02 Amikana. Biologics Faculté de Médecine de la Timone Methods for identifying antiretroviral compounds targeting the reverse transcriptase of hiv
EP3390640A4 (en) * 2015-12-17 2019-05-22 Evonik Degussa (China) Co., Ltd GENETASSETTE FOR KNOCK-OUT THROUGH HOMOLOGOUS RECOMBINATION IN HEF-CELLS

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US20030092183A1 (en) * 2001-09-21 2003-05-15 Fisher Katherine E. Rapid creation of gene targeting vectors using homologous recombination in yeast
US9493769B2 (en) 2004-06-02 2016-11-15 Universite D'aix-Marseille Yeast-based assay for measuring the functional activity of an HIV-1 protease in response to an antiviral agent
WO2009139349A1 (ja) * 2008-05-14 2009-11-19 Ｂｉｏ－ｅｎｅｒｇｙ株式会社 酵母細胞に遺伝子を導入する方法およびそのためのベクター

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
A new method for repeated self-cloning promoter replacement in saccharomyces cerevisiae;OLGA A.SOFYANOVICH,et al;《molecular biotechnology，part 》;20101219;第48卷(第3期);218－227 *
positive selection vectors;CHOI YOUNG-JUN,et al;《CRC CRITICAL REVIEWS IN BIOTECHNOLOGY》;20020101;第22卷(第3期);225－244 *
Production of a heterozygous mutant cell line by homologous recombination(single knockout);Mortensen R;《current protocols in molecular biology》;20010531;23.5.1－23.5.11 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112175984A (zh) * 2020-09-18 2021-01-05 中国科学院深圳先进技术研究院 基于合成基因和酿酒酵母同源重组机制的分子克隆方法
WO2022057094A1 (zh) * 2020-09-18 2022-03-24 中国科学院深圳先进技术研究院 基于合成基因和酿酒酵母同源重组机制的分子克隆方法

Also Published As

Publication number Publication date
JP2014519840A (ja) 2014-08-21
BR112013033177A2 (https=) 2012-12-27
US20140120624A1 (en) 2014-05-01
EP2721159A1 (en) 2014-04-23
CN103917648A (zh) 2014-07-09
BR112013033177A8 (pt) 2018-07-10
WO2012176016A1 (en) 2012-12-27
JP5973563B2 (ja) 2016-08-23
WO2012176016A8 (en) 2013-02-21

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