JP5935985B2 - 熱サイクル装置 - Google Patents
熱サイクル装置 Download PDFInfo
- Publication number
- JP5935985B2 JP5935985B2 JP2012079767A JP2012079767A JP5935985B2 JP 5935985 B2 JP5935985 B2 JP 5935985B2 JP 2012079767 A JP2012079767 A JP 2012079767A JP 2012079767 A JP2012079767 A JP 2012079767A JP 5935985 B2 JP5935985 B2 JP 5935985B2
- Authority
- JP
- Japan
- Prior art keywords
- heating unit
- temperature
- arrangement
- unit
- heating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000010438 heat treatment Methods 0.000 claims description 245
- 238000006243 chemical reaction Methods 0.000 claims description 153
- 238000000034 method Methods 0.000 claims description 66
- 239000012295 chemical reaction liquid Substances 0.000 claims description 55
- 230000008569 process Effects 0.000 claims description 51
- 230000007246 mechanism Effects 0.000 claims description 41
- 230000005484 gravity Effects 0.000 claims description 39
- 239000000243 solution Substances 0.000 description 46
- 238000003752 polymerase chain reaction Methods 0.000 description 33
- 238000005259 measurement Methods 0.000 description 28
- 239000007788 liquid Substances 0.000 description 22
- 239000000523 sample Substances 0.000 description 19
- 230000001276 controlling effect Effects 0.000 description 15
- 239000007850 fluorescent dye Substances 0.000 description 14
- 102100034343 Integrase Human genes 0.000 description 11
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 11
- 102000004190 Enzymes Human genes 0.000 description 10
- 108090000790 Enzymes Proteins 0.000 description 10
- 238000000137 annealing Methods 0.000 description 9
- 238000003757 reverse transcription PCR Methods 0.000 description 9
- 108020004414 DNA Proteins 0.000 description 8
- 206010069767 H1N1 influenza Diseases 0.000 description 7
- 208000009620 Orthomyxoviridae Infections Diseases 0.000 description 7
- 102000004167 Ribonuclease P Human genes 0.000 description 7
- 108090000621 Ribonuclease P Proteins 0.000 description 7
- 230000008859 change Effects 0.000 description 7
- 238000003780 insertion Methods 0.000 description 7
- 230000037431 insertion Effects 0.000 description 7
- 238000010839 reverse transcription Methods 0.000 description 7
- 201000010740 swine influenza Diseases 0.000 description 7
- 108020004707 nucleic acids Proteins 0.000 description 6
- 102000039446 nucleic acids Human genes 0.000 description 6
- 150000007523 nucleic acids Chemical class 0.000 description 6
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 5
- 230000000875 corresponding effect Effects 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 238000007789 sealing Methods 0.000 description 5
- 238000003505 heat denaturation Methods 0.000 description 4
- 208000037797 influenza A Diseases 0.000 description 4
- 238000003753 real-time PCR Methods 0.000 description 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical group [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 3
- 229910052782 aluminium Inorganic materials 0.000 description 3
- 239000012472 biological sample Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 238000003317 immunochromatography Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 229910052697 platinum Inorganic materials 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000005382 thermal cycling Methods 0.000 description 3
- 238000000018 DNA microarray Methods 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229910000881 Cu alloy Inorganic materials 0.000 description 1
- 239000005662 Paraffin oil Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 230000005674 electromagnetic induction Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000007849 hot-start PCR Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 206010022000 influenza Diseases 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 229920002545 silicone oil Polymers 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012079767A JP5935985B2 (ja) | 2012-03-30 | 2012-03-30 | 熱サイクル装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012079767A JP5935985B2 (ja) | 2012-03-30 | 2012-03-30 | 熱サイクル装置 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016095148A Division JP6206688B2 (ja) | 2016-05-11 | 2016-05-11 | 熱サイクル装置 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013208068A JP2013208068A (ja) | 2013-10-10 |
| JP2013208068A5 JP2013208068A5 (enExample) | 2015-05-07 |
| JP5935985B2 true JP5935985B2 (ja) | 2016-06-15 |
Family
ID=49526637
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012079767A Expired - Fee Related JP5935985B2 (ja) | 2012-03-30 | 2012-03-30 | 熱サイクル装置 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP5935985B2 (enExample) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016168018A (ja) * | 2015-03-13 | 2016-09-23 | セイコーエプソン株式会社 | 核酸増幅反応装置及び核酸増幅方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4949600B2 (ja) * | 2000-09-14 | 2012-06-13 | カリパー・ライフ・サイエンシズ・インク. | 温度介在反応を行うためのマイクロ流体装置及び方法 |
| JP2005034121A (ja) * | 2003-07-15 | 2005-02-10 | Nagasaki Prefecture | 食中毒起因細菌の一括検出方法及びその試薬 |
| AU2006204082A1 (en) * | 2005-01-04 | 2006-07-13 | Stratagene California | Hot start polymerase reaction using a thermolabile blocker |
| KR20100019409A (ko) * | 2007-01-22 | 2010-02-18 | 웨이퍼젠, 인크. | 고효율 화학 반응을 위한 장치 및 방법 |
| JP5196126B2 (ja) * | 2007-12-10 | 2013-05-15 | セイコーエプソン株式会社 | 生体試料反応装置および生体試料反応方法 |
| JP2011174734A (ja) * | 2010-02-23 | 2011-09-08 | Seiko Epson Corp | 検体反応装置及びバイオチップ |
-
2012
- 2012-03-30 JP JP2012079767A patent/JP5935985B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP2013208068A (ja) | 2013-10-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6216494B2 (ja) | 熱サイクル装置及び熱サイクル装置の制御方法 | |
| JP5967361B2 (ja) | 熱サイクル装置 | |
| JP5867668B2 (ja) | 熱サイクル装置及び熱サイクル方法 | |
| CN102653715B (zh) | 热循环装置 | |
| JP6206688B2 (ja) | 熱サイクル装置 | |
| JP5971463B2 (ja) | 熱サイクル装置及び熱サイクル装置の制御方法 | |
| JP5935985B2 (ja) | 熱サイクル装置 | |
| JP2013208066A (ja) | 熱サイクル装置及び熱サイクル装置の制御方法 | |
| JP5935980B2 (ja) | 熱サイクル装置 | |
| JP6090554B2 (ja) | 熱サイクル装置 | |
| US20150247186A1 (en) | Nucleic acid amplification method | |
| JP5896110B2 (ja) | 熱サイクル装置及び熱サイクル装置の制御方法 | |
| JP6120030B2 (ja) | 熱サイクル装置 | |
| JP5935981B2 (ja) | 熱サイクル装置 | |
| JP2013252091A (ja) | 熱サイクル装置 | |
| JP2014135942A (ja) | 熱サイクル装置及び熱サイクル方法 | |
| JP5853494B2 (ja) | 熱サイクル装置及び異常判定方法 | |
| JP2013240302A (ja) | 熱サイクル装置 | |
| JP5849443B2 (ja) | 熱サイクル装置 | |
| JP5842391B2 (ja) | 熱サイクル装置 | |
| JP2017112842A (ja) | 熱サイクル装置および熱サイクル装置の制御方法 | |
| JP2017163918A (ja) | 核酸増幅反応用カートリッジ、核酸増幅装置、および核酸増幅方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RD07 | Notification of extinguishment of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7427 Effective date: 20140619 |
|
| A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20150324 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20150324 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20151030 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20151125 |
|
| A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20160125 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20160413 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20160426 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 5935985 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| LAPS | Cancellation because of no payment of annual fees |