JP5912077B2 - スパイダーシルクタンパク質の重合体を産生する方法 - Google Patents

スパイダーシルクタンパク質の重合体を産生する方法 Download PDF

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Publication number
JP5912077B2
JP5912077B2 JP2012507182A JP2012507182A JP5912077B2 JP 5912077 B2 JP5912077 B2 JP 5912077B2 JP 2012507182 A JP2012507182 A JP 2012507182A JP 2012507182 A JP2012507182 A JP 2012507182A JP 5912077 B2 JP5912077 B2 JP 5912077B2
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Prior art keywords
spider silk
amino acid
silk protein
protein
acid residues
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JP2012507182A
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Japanese (ja)
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JP2012531889A5 (OSRAM
JP2012531889A (ja
Inventor
ヨハンソン,ヤン
ヘドハマー,ミー
ライジング,アンナ
ノードリング,シャスティン
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Spiber Technologies AB
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Spiber Technologies AB
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Priority claimed from EP09158445A external-priority patent/EP2243792A1/en
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2012507182A 2009-04-22 2010-04-21 スパイダーシルクタンパク質の重合体を産生する方法 Active JP5912077B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
EP09158445A EP2243792A1 (en) 2009-04-22 2009-04-22 Methods of producing polymers of spider silk proteins
EP09158445.8 2009-04-22
EP10156927 2010-03-18
EP10156927.5 2010-03-18
EP10156923.4 2010-03-18
EP10156923 2010-03-18
PCT/SE2010/050439 WO2010123450A1 (en) 2009-04-22 2010-04-21 Method of producing polymers of spider silk proteins

Publications (3)

Publication Number Publication Date
JP2012531889A JP2012531889A (ja) 2012-12-13
JP2012531889A5 JP2012531889A5 (OSRAM) 2013-06-20
JP5912077B2 true JP5912077B2 (ja) 2016-04-27

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ID=43011348

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JP2012507182A Active JP5912077B2 (ja) 2009-04-22 2010-04-21 スパイダーシルクタンパク質の重合体を産生する方法

Country Status (9)

Country Link
US (1) US8642734B2 (OSRAM)
EP (1) EP2421890B1 (OSRAM)
JP (1) JP5912077B2 (OSRAM)
CN (1) CN102395601A (OSRAM)
AU (1) AU2010239773B2 (OSRAM)
CA (1) CA2759465C (OSRAM)
DK (1) DK2421890T3 (OSRAM)
RU (1) RU2560437C2 (OSRAM)
WO (1) WO2010123450A1 (OSRAM)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2792476C (en) 2010-03-18 2018-12-11 Spiber Technologies Ab Production of proteins and polypeptides
DK3178843T3 (da) 2010-10-27 2019-08-19 Spiber Tech Ab Edderkoppesilke-fusionsproteinstrukturer til at binde til et organisk mål
CN103502516B (zh) * 2011-06-01 2015-04-22 丝芭博株式会社 人造多肽纤维及其制造方法
US9708376B2 (en) * 2012-05-02 2017-07-18 Spiber Technologies Ab Spider silk fusion protein structures without repetitive fragment for binding to an organic target
EP3046585B1 (en) 2013-09-17 2021-08-25 Bolt Threads, Inc. Methods and compositions for synthesizing improved silk fibers
WO2016090055A1 (en) 2014-12-02 2016-06-09 Silk Therapeutics, Inc. Silk performance apparel and products and methods of preparing the same
WO2016149414A1 (en) * 2015-03-16 2016-09-22 Bolt Threads, Inc. Improved silk fibers
US20180080147A1 (en) 2015-04-09 2018-03-22 Spiber Inc. Polar solvent solution and production method thereof
CN107735406A (zh) 2015-04-09 2018-02-23 丝芭博株式会社 极性溶剂溶液及其制造方法
EP3307765B1 (en) * 2015-06-11 2024-04-10 Bolt Threads, Inc. Recombinant protein fiber yarns with improved properties
CN105031723B (zh) * 2015-06-23 2017-05-24 上海交通大学 基于蜘蛛丝蛋白的温敏性水凝胶
US11512425B2 (en) 2015-07-14 2022-11-29 Evolved By Nature, Inc. Silk performance apparel and products and methods of preparing the same
JP2018159137A (ja) 2015-08-20 2018-10-11 国立研究開発法人理化学研究所 クモ糸様構造を有するポリペプチド繊維の製造方法
US10626152B2 (en) 2015-11-13 2020-04-21 Spiber Technologies Ab Charge-reversed N-terminal spider silk protein domain and uses thereof
GB201522610D0 (en) * 2015-12-22 2016-02-03 Univ Southampton Protein
CN109562200B (zh) * 2016-05-16 2022-09-09 思百博技术股份公司 固体表面的蜘蛛丝涂层
EP3469121A4 (en) * 2016-06-10 2020-01-08 Bolt Threads, Inc. RECOMBINANT PROTEIN FIBER YARNS WITH IMPROVED PROPERTIES
EP3263593A1 (en) 2016-07-01 2018-01-03 Anna Rising Engineered spider silk proteins and uses thereof
KR20190046993A (ko) 2016-09-14 2019-05-07 볼트 쓰레즈, 인크. 긴 균일한 재조합 단백질 섬유
JP7023950B2 (ja) * 2016-11-11 2022-02-22 アムシルク・ゲーエムベーハー センサとしての収縮性バイオポリマー繊維の使用
CN107475807A (zh) * 2017-08-30 2017-12-15 常州豫春化工有限公司 一种改性尼龙6纤维的制备方法
US12059826B2 (en) * 2017-09-26 2024-08-13 Spiber Technologies Ab Structuring of surface-active macromolecules
CN111712514A (zh) 2017-09-27 2020-09-25 自然进化公司 丝涂布织物和产品及其制备方法
JP2021035330A (ja) 2017-09-29 2021-03-04 Spiber株式会社 発現カセット
WO2021001840A1 (en) * 2019-07-04 2021-01-07 Seevix Material Sciences Ltd. Prokaryotic expression system and methods of using the same
CN110551194B (zh) * 2019-09-19 2023-03-21 天津大学 重组蜘蛛卵鞘丝蛋白复合物及其生成的人造卵鞘丝
WO2021214780A1 (en) 2020-04-23 2021-10-28 Seevix Material Sciences Ltd. Modified spider silk fiber and use thereof
WO2024091831A2 (en) * 2022-10-28 2024-05-02 National Taiwan University A recombinant spider silk protein and method for tag-free and time-saving purification thereof
CN218413490U (zh) 2022-10-31 2023-01-31 徐州龙安电子科技有限公司 一种新型蜘蛛电子标签
DE102023105437B3 (de) 2023-03-06 2024-06-13 Dr. Ing. H.C. F. Porsche Aktiengesellschaft Fahrzeugkomponente mit wenigstens einer Fahrzeuginnenraumkomponente

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003057727A1 (en) * 2002-01-11 2003-07-17 Nexia Biotechnologies, Inc. Methods of producing silk polypeptides and products thereof
AU2005263622B2 (en) * 2004-07-22 2012-04-26 Amsilk Gmbh Recombinant spider silk proteins
CN101395178B (zh) 2005-12-30 2013-05-01 思百博技术股份公司 蜘蛛丝蛋白和用于产生蜘蛛丝蛋白的方法
BRPI0701826B1 (pt) * 2007-03-16 2021-02-17 Embrapa - Empresa Brasileira De Pesquisa Agropecuária proteínas da teia de aranha nephilengys cruentata, avicularia juruensis e parawixia bistriata isoladas da biodiversidade brasileira
US9051383B2 (en) 2007-06-11 2015-06-09 The Regents Of The University Of California Spider silk dragline polynucleotides, polypeptides and methods of use thereof

Also Published As

Publication number Publication date
RU2011145429A (ru) 2013-05-27
WO2010123450A1 (en) 2010-10-28
CA2759465A1 (en) 2010-10-28
RU2560437C2 (ru) 2015-08-20
EP2421890B1 (en) 2015-08-26
AU2010239773A1 (en) 2011-11-03
US20120041177A1 (en) 2012-02-16
JP2012531889A (ja) 2012-12-13
EP2421890A4 (en) 2012-10-24
EP2421890A1 (en) 2012-02-29
DK2421890T3 (en) 2015-11-23
CA2759465C (en) 2017-12-12
CN102395601A (zh) 2012-03-28
AU2010239773B2 (en) 2014-11-06
US8642734B2 (en) 2014-02-04

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