JP5881801B2 - 腸内ポリアミン増強剤 - Google Patents
腸内ポリアミン増強剤 Download PDFInfo
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- JP5881801B2 JP5881801B2 JP2014220420A JP2014220420A JP5881801B2 JP 5881801 B2 JP5881801 B2 JP 5881801B2 JP 2014220420 A JP2014220420 A JP 2014220420A JP 2014220420 A JP2014220420 A JP 2014220420A JP 5881801 B2 JP5881801 B2 JP 5881801B2
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- polyamine
- enhancer
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- intestinal
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Description
分の探索を試みた。その結果、腸内のポリアミン濃度を増強する作用の高い成分をいくつか見出した。かかる知見に基づいて、本発明を完成させた。
(1)腸内細菌によるポリアミンの生成を促進する成分を少なくとも1種含有する、腸内のポリアミン濃度の増強剤。
(4)顆粒剤、粉剤、錠剤、カプセル剤、及びマイクロカプセル剤のいずれかの形態である、(1)〜(3)のいずれか1に記載の増強剤。
(6)(1)〜(5)のいずれか1に記載の増強剤を含有する食品。
(7)食品がヨーグルトである(6)に記載の食品。
(9)(1)〜(4)のいずれか1に記載の増強剤を含有する医薬品。
(10)アトピー性皮膚炎を治療するための(9)に記載の医薬品。
プ、バクテロイデス・フラギリス(Bacteroides fragilis)グループ、ビフィドバクテリウム属(Bifidobacterium)、アトポビウム(Atopobium)クラスター等が挙げられる。特に、クロストリディウム・コッコイデスグループとバクテロイデス・フラギリスグループの細菌全体に占める割合は高く、少なくともこの2グループで50%以上を占めることが
知られている。クロストリディウム・コッコイデスグループとしては、例えばクロストリディウム・クロストリディフォルムス(Clostridium clostridiiformes)、ブラウティア・プロダクタ(Blautia producta)(旧名:ルミノコッカス・プロダクタス(Ruminococcus productus))、及びユウバクテリウム・レクターレ(Eubacterium rectale)が挙げ
られる。バクテロイデス・フラギリスグループとしては、例えばバクテロイデス・テタイオタオミクロン(Bacteroides thetaiotaomicron)、バクテロイデス・ユニフォルミス(Bacteroides uniformis)、及びバクテロイデス・オバタス(Bacteroides ovatus)が挙
げられる。
また、本発明の組成物は、1以上の追加成分を配合して調製してもよい。追加成分の例としては、抗酸化剤、血糖降下剤、抗コレステロール剤、免疫賦活剤、ビタミン、アミノ酸、ペプチド、タンパク質、ミネラル分(鉄、亜鉛、マグネシム、ヨードなど)、脂肪酸(EPA、DHAなど)等を挙げることができる。
ができる。具体例としては、ビフィズス菌、乳酸菌等が挙げられる。
腸内の全代謝産物として解析用のサンプルとする。当該サンプルをメタボローム解析に供し、ピークを検出する。当該ピークの情報として、質量電荷比(m/z)、泳動時間(Migration time: MT)、及びピーク面積値を得る。各ピーク面積値の内部標準の面積値に
対する相対値を算出し、腸内の代謝産物中のポリアミン含量との相関性を検討する。これとは別に、各ピークについてのm/z及びMT値に基づいて、対応する成分を同定する。
い。
腸内全代謝産物解析(メタボローム解析)を用いて、腸内のポリアミン濃度を増強する可能性のある成分を糞便から探索した。
装置:Agilent CE-TOFMS system(Agilent Technologies社);
Capillary : Fused silica capillary i.d. 50 μm × 80 cm;
測定条件;
Run buffer : Cation Buffer Solution ( p/n : H3301-1001)
Rinse buffer : Cation Buffer Solution ( p/n : H3301-1001)
Sample injection : Pressure injection 50 mbar, 10 sec
CE voltage : Positive, 27 kV
MS ionization : ESI Positive
MS capillary voltage : 4,000 V
MS scan range : m/z 50-1,000
Sheath liquid : HMT Sheath Liquid ( p/n : H3301-1020)。
装置:Agilent CE-TOFMS system(Agilent Technologies 社);
Capillary : Fused silica capillary i.d. 50 μm × 80 cm;
測定条件;
Run buffer : Anion Buffer Solution ( p/n : H3302-1021)
Rinse buffer : Anion Buffer Solution ( p/n : H3302-1022)
Sample injection : Pressure injection 50 mbar, 25 sec
CE voltage : Positive, 30 kV
MS ionization : ESI Negative
MS capillary voltage : 3,500 V
MS scan range : m/z 50-1,000
Sheath liquid : HMT Sheath Liquid ( p/n : H3301-1020)。
m/z)、泳動時間(Migration time: MT)、及びピーク面積値を得た。当該各ピーク
面積値を内部標準の面積値で割り、相対値を算出し個体間の比較を行った。各ピークの相対値と、上記の方法で同様に算出したポリアミン(プトレッシン、スペルミジン、スペルミン)の相対値を比較検討した。一方、検出された各ピークについてのm/z及びMTの値をHMT代謝物質データベースと照合することにより、各ピークに対応する成分を同定した。
当該検討により、プトレッシンの濃度と危険率5%以下で正の相関性を有する成分が28
種類、スペルミジンの濃度と危険率5%以下で正の相関性を有する成分が23種類存在す
ることが示された(表1)。
試験例1においてポリアミンと濃度上の有意な相関性を示した成分が、腸内の常在菌によるポリアミンの生成を促進するか検討した。
トリディウム・コッコイデスグループとしてブラウティア・プロダクタJCM 1471T(旧名
:ルミノコッカス・プロダクツ)を用いた(独立行政法人 理化学研究所バイオリソースセンター微生物材料開発室より入手)。これら菌株をそれぞれEggerth Gagnon(EG)寒天培地にて37℃で48時間前培養し、GAM液体培地(日水製薬株式会社)にOD660値
が0.5になるよう懸濁した。
Claims (5)
- 腸内細菌によるプトレッシンの生成を促進するための腸内のポリアミン濃度の増強剤であって、
腸内細菌によるプトレッシンの生成を促進する成分を少なくとも1種含有し、前記成分が、アラニン、アルギニン、セリン、γ−アミノ酪酸、リンゴ酸、ロイシン、アデニン、ウラシル、イノシン、ヒポキサンチン又はこれらの組み合わせから選択される、
前記増強剤。 - 前記成分としてアルギニンを含んでなる、請求項1に記載の剤。
- 前記成分はアルギニンからなる、請求項1又は2に記載の増強剤。
- 胃内及び小腸上中部で溶解せず、小腸下部及び大腸で溶解する被覆層を有する、請求項1〜3のいずれか1項に記載の増強剤。
- 顆粒剤、粉剤、錠剤、カプセル剤、及びマイクロカプセル剤のいずれかの形態である、請求項1〜4のいずれか1項に記載の増強剤。
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