JP5846558B2 - 多能性幹細胞から骨格筋前駆細胞への分化誘導方法 - Google Patents

多能性幹細胞から骨格筋前駆細胞への分化誘導方法 Download PDF

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JP5846558B2
JP5846558B2 JP2011554336A JP2011554336A JP5846558B2 JP 5846558 B2 JP5846558 B2 JP 5846558B2 JP 2011554336 A JP2011554336 A JP 2011554336A JP 2011554336 A JP2011554336 A JP 2011554336A JP 5846558 B2 JP5846558 B2 JP 5846558B2
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cells
cell
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skeletal muscle
pdgfrα
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JP2012532585A (ja
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英俊 桜井
英俊 桜井
泰子 坂口
泰子 坂口
淳子 瀬原
淳子 瀬原
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Kyoto University NUC
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Rheumatology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Neurology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2011554336A 2009-07-09 2010-07-09 多能性幹細胞から骨格筋前駆細胞への分化誘導方法 Active JP5846558B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US27047909P 2009-07-09 2009-07-09
US61/270,479 2009-07-09
PCT/JP2010/062023 WO2011004911A1 (en) 2009-07-09 2010-07-09 Method of inducing differentiation from pluripotent stem cells to skeletal muscle progenitor cells

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JP2012532585A JP2012532585A (ja) 2012-12-20
JP2012532585A5 JP2012532585A5 (enExample) 2013-08-15
JP5846558B2 true JP5846558B2 (ja) 2016-01-20

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US (1) US20120164731A1 (enExample)
JP (1) JP5846558B2 (enExample)
WO (1) WO2011004911A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2315829B1 (en) 2008-08-08 2015-02-11 Mayo Foundation For Medical Education And Research Induced pluripotent stem cells
CA2760042C (en) 2009-04-27 2020-03-10 Ottawa Hospital Research Institute Compositions and methods for modulating stem cells and uses thereof
KR101334404B1 (ko) * 2011-04-28 2013-12-12 포항공과대학교 산학협력단 배아줄기세포 유래의 인공 마이크로베시클을 이용한 역분화 유도만능줄기세포의 제조방법
US20130029416A1 (en) 2011-07-22 2013-01-31 Tayaramma Thatava Differentiating induced pluripotent stem cells into glucose-responsive, insulin-secreting progeny
JP6570833B2 (ja) * 2011-08-29 2019-09-04 アンスティトゥー ナショナル ドゥ ラ サンテ エ ドゥ ラ レシェルシュ メディカル（イエヌエスエエールエム） 人工沿軸中胚葉前駆（ｉＰＡＭ）細胞を調製する方法及びその使用
JP5785619B2 (ja) 2011-09-15 2015-09-30 生化学工業株式会社 骨格筋再生促進剤
WO2015064585A1 (ja) * 2013-10-29 2015-05-07 国立大学法人熊本大学 筋疾患の治療または予防のための医薬組成物
US20170009210A1 (en) * 2014-02-05 2017-01-12 Mayo Foundation For Medical Education And Research Guided differentiation of induced pluripotent stem cells
US20170327791A1 (en) * 2014-11-14 2017-11-16 Jcr Pharmaceuticals Co., Ltd Muscular dystrophy therapeutic agent containing pluripotent stem cells derived from dental pulp
KR101636533B1 (ko) * 2014-12-22 2016-07-05 경북대학교 산학협력단 체세포로부터 근육줄기세포로의 역분화 유도용 조성물 및 이를 이용한 근육줄기세포의 제조방법
JP6845500B2 (ja) * 2014-12-29 2021-03-17 国立大学法人京都大学 骨格筋前駆細胞の製造方法
WO2016141084A1 (en) * 2015-03-03 2016-09-09 The Board Of Trustees Of The Leland Stanford Junior University Producing mesodermal cell types and methods of using the same
JP2017108705A (ja) * 2015-12-18 2017-06-22 国立大学法人京都大学 心筋細胞の製造方法
CA3044691A1 (en) 2016-10-26 2018-05-03 Genea Biocells USA (Holdings), Inc. Improved generation of muscle lineage cells and therapeutic uses thereof
CN111225974B (zh) * 2017-10-25 2021-09-17 雪拉托兹治疗株式会社 新型肌肉骨骼系统干细胞

Family Cites Families (3)

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Publication number Priority date Publication date Assignee Title
CA2626152A1 (en) * 2005-10-24 2007-05-03 Agency For Science, Technology And Research Methods of specifying mesodermal, endodermal and mesoendodermal cell fates
AU2008211103B2 (en) * 2007-01-30 2014-05-08 University Of Georgia Research Foundation, Inc. Early mesoderm cells, a stable population of mesendoderm cells that has utility for generation of endoderm and mesoderm lineages and multipotent migratory cells (MMC)
US9057051B2 (en) * 2008-10-31 2015-06-16 Katholieke Universiteit Leuven Optimized methods for differentiation of cells into cells with hepatocyte progenitor phenotypes, cells produced by the methods, and methods of using the cells

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WO2011004911A1 (en) 2011-01-13
US20120164731A1 (en) 2012-06-28
JP2012532585A (ja) 2012-12-20

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