JP5797405B2 - 二分子層 - Google Patents

二分子層 Download PDF

Info

Publication number
JP5797405B2
JP5797405B2 JP2010521469A JP2010521469A JP5797405B2 JP 5797405 B2 JP5797405 B2 JP 5797405B2 JP 2010521469 A JP2010521469 A JP 2010521469A JP 2010521469 A JP2010521469 A JP 2010521469A JP 5797405 B2 JP5797405 B2 JP 5797405B2
Authority
JP
Japan
Prior art keywords
bilayer
support
hydrophilic
membrane
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2010521469A
Other languages
English (en)
Japanese (ja)
Other versions
JP2010536551A (ja
JP2010536551A5 (https=
Inventor
マーク イアン ウォレス
マーク イアン ウォレス
アンドリュー ジョン へロン
アンドリュー ジョン へロン
マシュー アレクサンダー ホールデン
マシュー アレクサンダー ホールデン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ISISINNOVATION LIMITED
Original Assignee
ISISINNOVATION LIMITED
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ISISINNOVATION LIMITED filed Critical ISISINNOVATION LIMITED
Publication of JP2010536551A publication Critical patent/JP2010536551A/ja
Publication of JP2010536551A5 publication Critical patent/JP2010536551A5/ja
Application granted granted Critical
Publication of JP5797405B2 publication Critical patent/JP5797405B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/5432—Liposomes or microcapsules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
    • Y10T156/10—Methods of surface bonding and/or assembly therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
JP2010521469A 2007-08-21 2008-08-19 二分子層 Active JP5797405B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0716264.7 2007-08-21
GBGB0716264.7A GB0716264D0 (en) 2007-08-21 2007-08-21 Bilayers
PCT/GB2008/002805 WO2009024775A1 (en) 2007-08-21 2008-08-19 Bilayers

Publications (3)

Publication Number Publication Date
JP2010536551A JP2010536551A (ja) 2010-12-02
JP2010536551A5 JP2010536551A5 (https=) 2011-09-29
JP5797405B2 true JP5797405B2 (ja) 2015-10-21

Family

ID=38566709

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010521469A Active JP5797405B2 (ja) 2007-08-21 2008-08-19 二分子層

Country Status (7)

Country Link
US (2) US20110041978A1 (https=)
EP (1) EP2183594B1 (https=)
JP (1) JP5797405B2 (https=)
CN (1) CN101821626B (https=)
CA (1) CA2697024C (https=)
GB (1) GB0716264D0 (https=)
WO (1) WO2009024775A1 (https=)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT2347775T (pt) 2005-12-13 2020-07-14 The President And Fellows Of Harvard College Estruturas em andaime para transplante celular
WO2008102120A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
GB0913823D0 (en) * 2009-08-07 2009-09-16 Isis Innovation Bilayers
US8313956B2 (en) 2010-03-09 2012-11-20 Nokia Corporation Apparatus and associated methods
JP6104806B2 (ja) 2010-10-06 2017-03-29 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 材料に基づく細胞治療のための注射可能孔形成性ハイドロゲル
AU2012215135B9 (en) 2011-02-11 2017-03-09 Oxford Nanopore Technologies Limited Mutant pores
EP2697394A4 (en) 2011-04-12 2015-01-14 Electronic Biosciences Inc CHEMICALLY MODIFIED NANOPORA DEVICES AT A SPECIFIC SITE
US9675561B2 (en) 2011-04-28 2017-06-13 President And Fellows Of Harvard College Injectable cryogel vaccine devices and methods of use thereof
GB201119032D0 (en) 2011-11-03 2011-12-14 Isis Innovation Multisomes: encapsulated droplet networks
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
CN104244929B (zh) 2012-04-16 2017-04-05 哈佛学院董事会 用于调节免疫反应的介孔二氧化硅组合物
US20150204873A1 (en) * 2012-06-18 2015-07-23 Electonic Biosciences, Inc. Cell-free assay device and methods of use
GB201219196D0 (en) 2012-10-25 2012-12-12 Isis Innovation Droplet assembly method
GB201219201D0 (en) 2012-10-25 2012-12-12 Isis Innovation Hydrogel network
EP2911771B1 (en) * 2012-10-26 2017-01-18 Oxford Nanopore Technologies Limited Droplet interfaces
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
EP2928610A2 (en) * 2012-12-07 2015-10-14 Isis Innovation Limited Droplet assembly by 3d printing
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CN103435269A (zh) * 2013-08-21 2013-12-11 哈尔滨工业大学 一种固体支撑仿生膜体系的制备方法
JP6749243B2 (ja) 2014-01-22 2020-09-02 オックスフォード ナノポール テクノロジーズ リミテッド １つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法
JP7348708B2 (ja) 2014-04-30 2023-09-21 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 組み合わせワクチン装置および癌細胞を殺滅する方法
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
WO2016009180A1 (en) 2014-07-14 2016-01-21 Isis Innovation Limited Measurement of analytes with membrane channel molecules, and bilayer arrays
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
GB201418512D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Electrical device with detachable components
HK1247861A1 (zh) 2015-01-30 2018-10-05 President And Fellows Of Harvard College 用於癌症治疗的肿瘤周围和肿瘤内部材料
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
WO2016164705A1 (en) 2015-04-10 2016-10-13 Omar Abdel-Rahman Ali Immune cell trapping devices and methods for making and using the same
US11169138B2 (en) 2015-04-14 2021-11-09 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
US10641764B2 (en) 2015-07-02 2020-05-05 The University Of Massachusetts Membrane and droplet-interface bilayer systems and methods
US10481120B1 (en) 2015-08-06 2019-11-19 University Of Tennessee Research Foundation Droplet based measurement of specific capacitance and interfacial tensions
KR102837999B1 (ko) 2015-12-08 2025-07-24 카트호리이케 유니버시타이트 로이펜 변형된 나노세공, 그를 포함하는 조성물, 및 그의 용도
EP3411475B1 (en) 2016-02-06 2025-08-27 President and Fellows of Harvard College Recapitulating the hematopoietic niche to reconstitute immunity
US10371610B2 (en) * 2016-02-23 2019-08-06 Noul Co., Ltd. Contact-type patch, staining method using the same, and manufacturing method thereof
KR20170099738A (ko) 2016-02-23 2017-09-01 노을 주식회사 접촉식 염색 패치 및 그 제조 방법
KR102478639B1 (ko) 2016-02-23 2022-12-19 노을 주식회사 표지 물질을 저장하는 패치, 이를 이용하는 조직 진단 방법 및 장치
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
US10155979B2 (en) 2016-03-30 2018-12-18 Roche Molecular Systems, Inc. Non-destructive bilayer monitoring using measurement of bilayer response to electrical stimulus
US10233486B2 (en) 2016-03-30 2019-03-19 Roche Sequencing Solutions, Inc. Method for inline bilayer capacitance monitoring
US10465240B2 (en) 2016-03-30 2019-11-05 Roche Sequencing Solutions, Inc. Electrical enhancement of bilayer formation
WO2017174990A1 (en) 2016-04-06 2017-10-12 Oxford Nanopore Technologies Limited Mutant pore
GB201611770D0 (en) 2016-07-06 2016-08-17 Oxford Nanopore Tech Microfluidic device
CN115404196A (zh) 2016-07-13 2022-11-29 哈佛学院院长等 抗原呈递细胞模拟支架及其制备和使用方法
CN110418651A (zh) 2016-08-02 2019-11-05 哈佛学院院长等 用于调节免疫应答的生物材料
GB201615741D0 (en) * 2016-09-15 2016-11-02 Univ College Cardiff Consultants Ltd Artificial cells
FR3061440B1 (fr) * 2017-01-05 2021-02-12 Centre Nat Rech Scient Procede de fabrication d’une membrane multicouche sur support solide a base de copolymere a blocs amphiphile
CN108329471A (zh) * 2017-01-19 2018-07-27 天津大学 一种锂硫电池正极活性材料异戊烯醇-硫共聚物及制备方法
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
WO2019002893A1 (en) 2017-06-30 2019-01-03 Vib Vzw NEW PROTEIN PORES
JP7058858B2 (ja) * 2017-10-19 2022-04-25 地方独立行政法人神奈川県立産業技術総合研究所 脂質二重膜形成器具及びそれを用いた脂質二重膜形成方法
DE102017126732A1 (de) * 2017-11-14 2019-05-16 Endress+Hauser Conducta Gmbh+Co. Kg Sensormembran, Sensor, ein Messgerät umfassend den Sensor und Verwendung des Sensors
GB2568895B (en) 2017-11-29 2021-10-27 Oxford Nanopore Tech Ltd Microfluidic device
GB2569630B (en) 2017-12-21 2022-10-12 Sharp Life Science Eu Ltd Droplet Interfaces in Electro-wetting Devices
GB2574048B (en) 2018-05-24 2021-06-16 Oxford Nanopore Tech Ltd Nanopore sensor component with electrostatic discharge protection
WO2020061129A1 (en) 2018-09-19 2020-03-26 President And Fellows Of Harvard College Compositions and methods for labeling and modulation of cells in vitro and in vivo
US11789006B2 (en) 2019-03-12 2023-10-17 Oxford Nanopore Technologies Plc Nanopore sensing device, components and method of operation
US12135326B2 (en) 2019-05-29 2024-11-05 Nanjing University Detection of analytes by nanopore without using electrodes
WO2021061837A1 (en) 2019-09-23 2021-04-01 President And Fellows Of Harvard College Biomaterial-based antigen free vaccine and the use thereof
CN112831395B (zh) * 2019-11-25 2024-01-16 深圳华大生命科学研究院 用于纳米孔测序的类细胞膜
GB202004944D0 (en) 2020-04-03 2020-05-20 King S College London Method
WO2022013551A1 (en) 2020-07-17 2022-01-20 Oxford Nanopore Technologies Limited Nanopore sensing device

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH067737A (ja) * 1992-06-30 1994-01-18 Junjiro Aoki 累積膜の連続製造方法
EP1373550A1 (en) * 2001-03-14 2004-01-02 Biacore AB Method of preparing supported lipid film membranes and use thereof
JP4394916B2 (ja) * 2003-09-19 2010-01-06 独立行政法人科学技術振興機構 人工脂質二重膜の形成装置および人工脂質二重膜の形成方法、並びにその利用
JP4394917B2 (ja) * 2003-09-19 2010-01-06 独立行政法人科学技術振興機構 人工脂質二重膜を有する電流測定装置
JP2005114576A (ja) * 2003-10-08 2005-04-28 Hitachi Software Eng Co Ltd 両親媒性分子固定化ビーズ、その製造方法、及びキャピラリビーズアレイのビーズ配列方法
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US8211712B2 (en) 2005-03-29 2012-07-03 The Board Of Trustees Of The Leland Stanford Junior University Method of fabricating lipid bilayer membranes on solid supports
ATE457823T1 (de) 2005-05-20 2010-03-15 Aquaporin Aps Membran zum filtrieren von wasser
JP5114702B2 (ja) 2005-07-29 2013-01-09 国立大学法人 東京大学 両親媒性単分子膜の接触による二分子膜の形成方法およびその装置
EP1931981A1 (en) 2005-09-07 2008-06-18 Agresearch Limited A method of manufacture
GB0614835D0 (en) * 2006-07-26 2006-09-06 Isis Innovation Formation of bilayers of amphipathic molecules

Also Published As

Publication number Publication date
US20130129910A1 (en) 2013-05-23
US20110041978A1 (en) 2011-02-24
CN101821626B (zh) 2015-04-08
EP2183594B1 (en) 2018-11-07
US8784929B2 (en) 2014-07-22
EP2183594A1 (en) 2010-05-12
CN101821626A (zh) 2010-09-01
JP2010536551A (ja) 2010-12-02
CA2697024C (en) 2016-10-18
WO2009024775A1 (en) 2009-02-26
WO2009024775A8 (en) 2009-12-03
CA2697024A1 (en) 2009-02-26
GB0716264D0 (en) 2007-09-26

Similar Documents

Publication Publication Date Title
JP5797405B2 (ja) 二分子層
CN102687021B (zh) 双层
Heron et al. Direct detection of membrane channels from gels using water-in-oil droplet bilayers
US7201836B2 (en) Multiaperture sample positioning and analysis system
US7387715B2 (en) Sample positioning and analysis system
CN1902497B (zh) 分析芯片以及所述分析芯片用于测定分子结构和功能的用途
JP4394916B2 (ja) 人工脂質二重膜の形成装置および人工脂質二重膜の形成方法、並びにその利用
Shoji et al. Spatially resolved chemical detection with a nanoneedle-probe-supported biological nanopore
JP4394917B2 (ja) 人工脂質二重膜を有する電流測定装置
WO2002024862A2 (en) Sample positioning and analysis system
JP2007527540A (ja) 細胞の体積変化を測定するための方法及び装置
EP3164712B1 (en) Microfluidic array supporting a lipid bilayer assembly
Danelon et al. Probing the function of ionotropic and G protein-coupled receptors in surface-confined membranes
Craighead Future lab-on-a-chip technologies for interrogating individual molecules
Halža et al. Well-defined microapertures for ion channel biosensors
JP2007503207A (ja) ギャップ結合を含有する生化学活性検出用装置
US20240226882A1 (en) Plasma Membrane on a Chip
Huang Engineering biological nanopores for proteomics study
Upadhye Developing instrumentation for multi-parametric investigation of mechanisms of mechanosensitivity in ion channels
Stimberg Microfluidic platform for bilayer experimentation–From a research

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110805

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20110805

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20120120

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20120207

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20120507

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20120514

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20120604

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20120611

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20120705

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20120712

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130312

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20140401

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20140701

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20140708

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140801

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20140826

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20141226

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20150122

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20150219

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150407

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150622

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20150721

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20150819

R150 Certificate of patent or registration of utility model

Ref document number: 5797405

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

S531 Written request for registration of change of domicile

Free format text: JAPANESE INTERMEDIATE CODE: R313531

S533 Written request for registration of change of name

Free format text: JAPANESE INTERMEDIATE CODE: R313533

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250