CN101821626B - 双层 - Google Patents

双层 Download PDF

Info

Publication number
CN101821626B
CN101821626B CN200880111401.7A CN200880111401A CN101821626B CN 101821626 B CN101821626 B CN 101821626B CN 200880111401 A CN200880111401 A CN 200880111401A CN 101821626 B CN101821626 B CN 101821626B
Authority
CN
China
Prior art keywords
bilayer
hydrated support
lipid
hydrated
hydrophilic body
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN200880111401.7A
Other languages
English (en)
Chinese (zh)
Other versions
CN101821626A (zh
Inventor
M·I·华莱士
A·J·赫伦
M·A·霍尔登
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford Innovation Co Ltd, University of
Original Assignee
Es Es Invention Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Es Es Invention Co ltd filed Critical Es Es Invention Co ltd
Publication of CN101821626A publication Critical patent/CN101821626A/zh
Application granted granted Critical
Publication of CN101821626B publication Critical patent/CN101821626B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/5432—Liposomes or microcapsules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
    • Y10T156/10—Methods of surface bonding and/or assembly therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicinal Preparation (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CN200880111401.7A 2007-08-21 2008-08-19 双层 Active CN101821626B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0716264.7 2007-08-21
GBGB0716264.7A GB0716264D0 (en) 2007-08-21 2007-08-21 Bilayers
PCT/GB2008/002805 WO2009024775A1 (en) 2007-08-21 2008-08-19 Bilayers

Publications (2)

Publication Number Publication Date
CN101821626A CN101821626A (zh) 2010-09-01
CN101821626B true CN101821626B (zh) 2015-04-08

Family

ID=38566709

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200880111401.7A Active CN101821626B (zh) 2007-08-21 2008-08-19 双层

Country Status (7)

Country Link
US (2) US20110041978A1 (https=)
EP (1) EP2183594B1 (https=)
JP (1) JP5797405B2 (https=)
CN (1) CN101821626B (https=)
CA (1) CA2697024C (https=)
GB (1) GB0716264D0 (https=)
WO (1) WO2009024775A1 (https=)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007070660A2 (en) 2005-12-13 2007-06-21 President And Fellows Of Harvard College Scaffolds for cell transplantation
US20110121840A1 (en) 2007-02-20 2011-05-26 Gurdial Singh Sanghera Lipid Bilayer Sensor System
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
GB0913823D0 (en) * 2009-08-07 2009-09-16 Isis Innovation Bilayers
US8313956B2 (en) * 2010-03-09 2012-11-20 Nokia Corporation Apparatus and associated methods
CN107648668B (zh) 2010-10-06 2021-06-18 哈佛学院董事会 用于基于材料的细胞疗法的可注射的成孔水凝胶
KR101939420B1 (ko) 2011-02-11 2019-01-16 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 세공
EP2697394A4 (en) 2011-04-12 2015-01-14 Electronic Biosciences Inc CHEMICALLY MODIFIED NANOPORA DEVICES AT A SPECIFIC SITE
US9675561B2 (en) 2011-04-28 2017-06-13 President And Fellows Of Harvard College Injectable cryogel vaccine devices and methods of use thereof
GB201119032D0 (en) 2011-11-03 2011-12-14 Isis Innovation Multisomes: encapsulated droplet networks
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
DK2838515T3 (da) 2012-04-16 2020-02-24 Harvard College Mesoporøse siliciumdioxidsammensætninger til modulering af immunresponser
EP2861998B1 (en) * 2012-06-18 2020-07-22 Electronic Biosciences Inc. Cell-free assay device and methods of use
GB201219196D0 (en) 2012-10-25 2012-12-12 Isis Innovation Droplet assembly method
GB201219201D0 (en) 2012-10-25 2012-12-12 Isis Innovation Hydrogel network
JP6375301B2 (ja) * 2012-10-26 2018-08-15 オックスフォード ナノポール テクノロジーズ リミテッド 液滴界面
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
KR102449271B1 (ko) 2012-12-07 2022-09-29 옥스포드 유니버시티 이노베이션 리미티드 3d 프린팅에 의한 비말 어셈블리
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CN103435269A (zh) * 2013-08-21 2013-12-11 哈尔滨工业大学 一种固体支撑仿生膜体系的制备方法
CN111534504B (zh) 2014-01-22 2024-06-21 牛津纳米孔科技公开有限公司 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法
EP3137105A4 (en) 2014-04-30 2017-12-27 President and Fellows of Harvard College Combination vaccine devices and methods of killing cancer cells
EP3137490B1 (en) 2014-05-02 2021-01-27 Oxford Nanopore Technologies Limited Mutant pores
WO2016009180A1 (en) 2014-07-14 2016-01-21 Isis Innovation Limited Measurement of analytes with membrane channel molecules, and bilayer arrays
CA2959220A1 (en) 2014-09-01 2016-03-10 Vib Vzw Mutant csgg pores
EP3204511B1 (en) 2014-10-07 2021-07-28 Oxford Nanopore Technologies Limited Mutant pores
GB201418512D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Electrical device with detachable components
US11786457B2 (en) 2015-01-30 2023-10-17 President And Fellows Of Harvard College Peritumoral and intratumoral materials for cancer therapy
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
WO2016164705A1 (en) 2015-04-10 2016-10-13 Omar Abdel-Rahman Ali Immune cell trapping devices and methods for making and using the same
WO2016166232A1 (en) 2015-04-14 2016-10-20 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
US10641764B2 (en) 2015-07-02 2020-05-05 The University Of Massachusetts Membrane and droplet-interface bilayer systems and methods
US10481120B1 (en) 2015-08-06 2019-11-19 University Of Tennessee Research Foundation Droplet based measurement of specific capacitance and interfacial tensions
AU2016369071B2 (en) 2015-12-08 2022-05-19 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
US11752238B2 (en) 2016-02-06 2023-09-12 President And Fellows Of Harvard College Recapitulating the hematopoietic niche to reconstitute immunity
US10371610B2 (en) 2016-02-23 2019-08-06 Noul Co., Ltd. Contact-type patch, staining method using the same, and manufacturing method thereof
KR20170099739A (ko) 2016-02-23 2017-09-01 노을 주식회사 접촉식 염색 보조 패치, 그 제조 방법 및 이를 이용하는 염색 방법
KR102478639B1 (ko) 2016-02-23 2022-12-19 노을 주식회사 표지 물질을 저장하는 패치, 이를 이용하는 조직 진단 방법 및 장치
CN116200476A (zh) 2016-03-02 2023-06-02 牛津纳米孔科技公开有限公司 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔
US10155979B2 (en) 2016-03-30 2018-12-18 Roche Molecular Systems, Inc. Non-destructive bilayer monitoring using measurement of bilayer response to electrical stimulus
US10465240B2 (en) 2016-03-30 2019-11-05 Roche Sequencing Solutions, Inc. Electrical enhancement of bilayer formation
US10233486B2 (en) 2016-03-30 2019-03-19 Roche Sequencing Solutions, Inc. Method for inline bilayer capacitance monitoring
EP4397970A3 (en) 2016-04-06 2024-10-09 Oxford Nanopore Technologies PLC Mutant pore
GB201611770D0 (en) 2016-07-06 2016-08-17 Oxford Nanopore Tech Microfluidic device
CN109789092A (zh) 2016-07-13 2019-05-21 哈佛学院院长等 抗原呈递细胞模拟支架及其制备和使用方法
IL264557B2 (en) 2016-08-02 2024-01-01 Harvard College Biological agents for regulating immune responses
GB201615741D0 (en) 2016-09-15 2016-11-02 Univ College Cardiff Consultants Ltd Artificial cells
FR3061440B1 (fr) * 2017-01-05 2021-02-12 Centre Nat Rech Scient Procede de fabrication d’une membrane multicouche sur support solide a base de copolymere a blocs amphiphile
CN108329471A (zh) * 2017-01-19 2018-07-27 天津大学 一种锂硫电池正极活性材料异戊烯醇-硫共聚物及制备方法
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN117106038B (zh) 2017-06-30 2025-12-09 弗拉芒区生物技术研究所 新颖蛋白孔
JP7058858B2 (ja) * 2017-10-19 2022-04-25 地方独立行政法人神奈川県立産業技術総合研究所 脂質二重膜形成器具及びそれを用いた脂質二重膜形成方法
DE102017126732A1 (de) * 2017-11-14 2019-05-16 Endress+Hauser Conducta Gmbh+Co. Kg Sensormembran, Sensor, ein Messgerät umfassend den Sensor und Verwendung des Sensors
GB2568895B (en) 2017-11-29 2021-10-27 Oxford Nanopore Tech Ltd Microfluidic device
GB2569630B (en) 2017-12-21 2022-10-12 Sharp Life Science Eu Ltd Droplet Interfaces in Electro-wetting Devices
GB2574048B (en) 2018-05-24 2021-06-16 Oxford Nanopore Tech Ltd Nanopore sensor component with electrostatic discharge protection
WO2020061129A1 (en) 2018-09-19 2020-03-26 President And Fellows Of Harvard College Compositions and methods for labeling and modulation of cells in vitro and in vivo
JP7492200B2 (ja) 2019-03-12 2024-05-29 オックスフォード ナノポール テクノロジーズ ピーエルシー ナノ細孔センシングデバイスと操作方法およびその成形方法
CN113574388B (zh) 2019-05-29 2025-12-05 南京大学 不使用电极的纳米孔单分子分析物检测
WO2021061837A1 (en) 2019-09-23 2021-04-01 President And Fellows Of Harvard College Biomaterial-based antigen free vaccine and the use thereof
CN112831395B (zh) * 2019-11-25 2024-01-16 深圳华大生命科学研究院 用于纳米孔测序的类细胞膜
GB202004944D0 (en) 2020-04-03 2020-05-20 King S College London Method
EP4675256A3 (en) 2020-07-17 2026-01-28 Oxford Nanopore Technologies PLC Nanopore sensing device

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002072873A1 (en) * 2001-03-14 2002-09-19 Biacore Ab Method of preparing supported lipid film membranes and use thereof
WO2008012552A1 (en) * 2006-07-26 2008-01-31 Isis Innovation Limited Formation of bilayers of amphipathic molecules

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH067737A (ja) * 1992-06-30 1994-01-18 Junjiro Aoki 累積膜の連続製造方法
JP4394917B2 (ja) * 2003-09-19 2010-01-06 独立行政法人科学技術振興機構 人工脂質二重膜を有する電流測定装置
JP4394916B2 (ja) * 2003-09-19 2010-01-06 独立行政法人科学技術振興機構 人工脂質二重膜の形成装置および人工脂質二重膜の形成方法、並びにその利用
JP2005114576A (ja) * 2003-10-08 2005-04-28 Hitachi Software Eng Co Ltd 両親媒性分子固定化ビーズ、その製造方法、及びキャピラリビーズアレイのビーズ配列方法
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US8211712B2 (en) 2005-03-29 2012-07-03 The Board Of Trustees Of The Leland Stanford Junior University Method of fabricating lipid bilayer membranes on solid supports
DE602006012307D1 (de) 2005-05-20 2010-04-01 Aquaporin Aps Membran zum filtrieren von wasser
JP5114702B2 (ja) 2005-07-29 2013-01-09 国立大学法人 東京大学 両親媒性単分子膜の接触による二分子膜の形成方法およびその装置
US20080318326A1 (en) 2005-09-07 2008-12-25 Agresearch Limited Method of Manufacture

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002072873A1 (en) * 2001-03-14 2002-09-19 Biacore Ab Method of preparing supported lipid film membranes and use thereof
WO2008012552A1 (en) * 2006-07-26 2008-01-31 Isis Innovation Limited Formation of bilayers of amphipathic molecules

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
A novel method for artificial lipid-bilayer formation;Toru Ide et al;《Biosensors and Bioelectronics》;20050126;672–677 *
Direct Detection of Membrane Channels from Gels Using Water-in-Oil Droplet Bilayers;Andrew J. Heron et al;《J. AM. CHEM. SOC.》;20071201;16042-16047 *
Enhanced Stability and Fluidity in Droplet on Hydrogel Bilayers for Measuring Membrane Protein Diffusion;James R. Thompson et al;《NANO Letters》;20071103;A-D *
Functional bionetworks from nanoliter water droplets;Holden MA et al;《J Am Chem Soc》;20070711;摘要、第8651页左栏第2段至第8652页左栏第3段、第8654页右栏第2-3段、第S1页第1段至第S3页第1段、图1-2、S1 *

Also Published As

Publication number Publication date
WO2009024775A8 (en) 2009-12-03
GB0716264D0 (en) 2007-09-26
CN101821626A (zh) 2010-09-01
EP2183594A1 (en) 2010-05-12
WO2009024775A1 (en) 2009-02-26
JP5797405B2 (ja) 2015-10-21
US8784929B2 (en) 2014-07-22
EP2183594B1 (en) 2018-11-07
US20130129910A1 (en) 2013-05-23
CA2697024C (en) 2016-10-18
JP2010536551A (ja) 2010-12-02
US20110041978A1 (en) 2011-02-24
CA2697024A1 (en) 2009-02-26

Similar Documents

Publication Publication Date Title
CN101821626B (zh) 双层
CN102687021B (zh) 双层
US7387715B2 (en) Sample positioning and analysis system
US7201836B2 (en) Multiaperture sample positioning and analysis system
Bayley et al. Droplet interface bilayers
US8268627B2 (en) Formation of bilayers of amphipathic molecules
US20090153153A1 (en) Multiaperture sample positioning and analysis system
US7858030B2 (en) Artificial lipid bilayer membrane formation device, artificial lipid bilayer membrane formation method, and usage thereof
JP2024119899A (ja) 細胞の微小液滴検出のためのデバイスおよび方法
US7408359B2 (en) Current measuring device having artificial lipid bilayer membrane
WO2002024862A2 (en) Sample positioning and analysis system
EP3164712B1 (en) Microfluidic array supporting a lipid bilayer assembly
Kramer et al. From LUVs to GUVs─ How to cover micrometer-sized pores with membranes
Craighead Future lab-on-a-chip technologies for interrogating individual molecules
Stimberg Microfluidic platform for bilayer experimentation–From a research
EP2444808A1 (en) Method for fusion of lipid bilayers

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
C56 Change in the name or address of the patentee
CP01 Change in the name or title of a patent holder

Address after: oxford

Patentee after: University of Oxford Innovation Co., Ltd.

Address before: oxford

Patentee before: ISIS Innovation