JP5789513B2 - シナプス小胞サイクリングアッセイおよびシステム - Google Patents

シナプス小胞サイクリングアッセイおよびシステム Download PDF

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JP5789513B2
JP5789513B2 JP2011526037A JP2011526037A JP5789513B2 JP 5789513 B2 JP5789513 B2 JP 5789513B2 JP 2011526037 A JP2011526037 A JP 2011526037A JP 2011526037 A JP2011526037 A JP 2011526037A JP 5789513 B2 JP5789513 B2 JP 5789513B2
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synaptic vesicle
electrode
synaptic
wells
platform
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JP2012501646A (ja
JP2012501646A5 (enExample
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ガーバー,デービット,ジェー.
コットレル,ジェフリー,アール.
ライアン,ティモシー,エー.
レベンソン,ジョナサン,エム.
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ガレニア コーポレーション
ガレニア コーポレーション
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5058Neurological cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00Screening for compounds of potential therapeutic value
    • G01N2500/10Screening for compounds of potential therapeutic value involving cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
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  • Analytical Chemistry (AREA)
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  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Electrochemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
  • Peptides Or Proteins (AREA)
JP2011526037A 2008-09-04 2009-08-31 シナプス小胞サイクリングアッセイおよびシステム Expired - Fee Related JP5789513B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9436108P 2008-09-04 2008-09-04
US61/094,361 2008-09-04
PCT/US2009/004932 WO2010027446A2 (en) 2008-09-04 2009-08-31 Synaptic vesicle cycling assays and systems

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JP2015151623A Division JP6106225B2 (ja) 2008-09-04 2015-07-31 シナプス小胞サイクリングアッセイおよびシステム

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JP2012501646A JP2012501646A (ja) 2012-01-26
JP2012501646A5 JP2012501646A5 (enExample) 2012-10-18
JP5789513B2 true JP5789513B2 (ja) 2015-10-07

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EP (1) EP2329275A2 (enExample)
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KR (1) KR20110073494A (enExample)
CN (1) CN102203622A (enExample)
AU (2) AU2009288657A1 (enExample)
CA (1) CA2744804C (enExample)
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Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011511665A (ja) * 2008-02-04 2011-04-14 バイエル・ヘルスケア・エルエルシー 半導体を素材とする分析対象物センサー及び方法
US9217155B2 (en) 2008-05-28 2015-12-22 University Of Massachusetts Isolation of novel AAV'S and uses thereof
US8734809B2 (en) 2009-05-28 2014-05-27 University Of Massachusetts AAV's and uses thereof
US9057734B2 (en) 2010-08-23 2015-06-16 President And Fellows Of Harvard College Optogenetic probes for measuring membrane potential
US9207237B2 (en) 2010-08-23 2015-12-08 President And Fellows Of Harvard College Systems, methods, and workflows for optogenetics analysis
US9488637B2 (en) * 2011-01-26 2016-11-08 The Curators Of The University Of Missouri Combination of single-cell electroporation and electrical recording using the same electrode
WO2012145624A2 (en) 2011-04-21 2012-10-26 University Of Massachusetts Raav-based compositions and methods for treating alpha-1 anti-trypsin deficiencies
US20150004637A1 (en) * 2011-11-23 2015-01-01 President And Fellows Of Harvard College Systems and methods for imaging at high spatial and/or temporal precision
US9696275B2 (en) 2012-10-12 2017-07-04 The Curators Of The University Of Missouri Addressable electrode arrays in multiple fluidic compartments and uses thereof
WO2014065330A1 (ja) 2012-10-25 2014-05-01 浜松ホトニクス株式会社 細胞観察装置、電気刺激装置、及び細胞観察方法
WO2014065329A1 (ja) * 2012-10-25 2014-05-01 浜松ホトニクス株式会社 細胞観察装置及び細胞観察方法
JP6370544B2 (ja) * 2013-11-11 2018-08-08 浜松ホトニクス株式会社 細胞観察装置及び細胞観察方法
EP3095853B1 (en) 2014-01-16 2019-01-30 Hamamatsu Photonics K.K. Cell observation device, electrostimulation device, and cell observation method
WO2015127128A2 (en) 2014-02-19 2015-08-27 University Of Massachusetts Recombinant aavs having useful transcytosis properties
DK3119797T3 (da) 2014-03-18 2021-03-15 Univ Massachusetts Raav-baserede sammensætninger og fremgangsmåder til behandling af amyotrofisk lateralsklerose
JP2017521044A (ja) * 2014-04-22 2017-08-03 キュー−ステート バイオサイエンシーズ, インコーポレイテッドQ−State Biosciences, Inc. 疼痛及び感覚障害のための化合物の分析
US10975391B2 (en) 2014-04-25 2021-04-13 University Of Massachusetts Recombinant AAV vectors useful for reducing immunity against transgene products
WO2015195769A2 (en) 2014-06-18 2015-12-23 President And Fellows Of Harvard College Optogenetic probes for measuring membrane potential
WO2016033501A1 (en) 2014-08-28 2016-03-03 Robert John Petcavich Method of fabricating cell arrays and uses thereof
US10711270B2 (en) 2014-10-03 2020-07-14 University Of Massachusetts High efficiency library-identified AAV vectors
US10370432B2 (en) 2014-10-03 2019-08-06 University Of Massachusetts Heterologous targeting peptide grafted AAVS
RU2020140209A (ru) 2014-10-21 2021-01-25 Юниверсити Оф Массачусетс Варианты рекомбинантных aav и их применения
US10077463B2 (en) 2015-01-15 2018-09-18 President And Fellows Of Harvard College Optical selection of cells
EP3256170B1 (en) 2015-02-13 2020-09-23 University of Massachusetts Compositions and methods for transient delivery of nucleases
US10048275B2 (en) 2015-03-13 2018-08-14 Q-State Biosciences, Inc. Cardiotoxicity screening methods
US11020443B2 (en) 2015-04-23 2021-06-01 University Of Massachusetts Modulation of AAV vector transgene expression
WO2016172008A1 (en) 2015-04-24 2016-10-27 University Of Massachusetts Modified aav constructions and uses thereof
US11248212B2 (en) 2015-06-30 2022-02-15 StemoniX Inc. Surface energy directed cell self assembly
US10760053B2 (en) 2015-10-15 2020-09-01 StemoniX Inc. Method of manufacturing or differentiating mammalian pluripotent stem cells or progenitor cells using a hollow fiber bioreactor
US11426469B2 (en) 2015-10-22 2022-08-30 University Of Massachusetts Prostate-targeting adeno-associated virus serotype vectors
CA3002982A1 (en) 2015-10-22 2017-04-27 University Of Massachusetts Methods and compositions for treating metabolic imbalance in neurodegenerative disease
US11826433B2 (en) 2016-02-02 2023-11-28 University Of Massachusetts Method to enhance the efficiency of systemic AAV gene delivery to the central nervous system
CA3012344A1 (en) 2016-02-12 2017-08-17 University Of Massachusetts Anti-angiogenic mirna therapeutics for inhibiting corneal neovascularization
EP3225279B1 (en) * 2016-03-31 2023-12-20 Université de Rennes Brain tissue stimulation apparatus and computer program
WO2017176929A1 (en) 2016-04-05 2017-10-12 University Of Massachusetts Compositions and methods for selective inhibition of grainyhead-like protein expression
US11413356B2 (en) 2016-04-15 2022-08-16 University Of Massachusetts Methods and compositions for treating metabolic imbalance
US11882815B2 (en) 2016-06-15 2024-01-30 University Of Massachusetts Recombinant adeno-associated viruses for delivering gene editing molecules to embryonic cells
US10457940B2 (en) 2016-09-22 2019-10-29 University Of Massachusetts AAV treatment of Huntington's disease
AU2017341849B2 (en) 2016-10-13 2024-03-21 University Of Massachusetts AAV capsid designs
JP7327803B2 (ja) 2017-05-09 2023-08-16 ユニバーシティ オブ マサチューセッツ 筋萎縮性側索硬化症(als)を処置する方法
CN111448321A (zh) 2017-09-22 2020-07-24 马萨诸塞大学 Sod1双表达载体及其用途
CA3096102A1 (en) 2018-04-13 2019-10-17 University Of Massachusetts Bicistronic aav vectors encoding hexosaminidase alpha and beta-subunits and uses thereof
JP7065433B2 (ja) * 2018-08-14 2022-05-12 日本電信電話株式会社 電極及びその製造方法、並びに積層体
KR20210087462A (ko) 2018-10-05 2021-07-12 유니버시티 오브 매사추세츠 Gm1 및 gm2 강글리오시드증의 치료를 위한 raav 벡터
SG11202111169YA (en) * 2019-07-16 2021-11-29 Meso Scale Technologies Llc Assay apparatuses, methods and reagents
EP4271789A4 (en) * 2020-12-31 2024-11-27 Q-State Biosciences, Inc. PLATE IMAGE PRODUCER
JP2024501848A (ja) 2020-12-31 2024-01-16 キュー-ステート バイオサイエンシーズ, インコーポレイテッド 光遺伝学のためのイメージャの較正および正規化
EP4271982A4 (en) * 2020-12-31 2024-11-27 Q-State Biosciences, Inc. Optical plate controller and reader
EP4271978A4 (en) 2020-12-31 2024-12-18 Q-State Biosciences, Inc. MICROSCOPE WITH SPATIAL IMAGING AND BEAM HOMOGENIZER
JP2024516303A (ja) * 2021-05-04 2024-04-12 キュー-ステート バイオサイエンシーズ, インコーポレイテッド 薬物フィンガプリンティング
JP2024525493A (ja) 2021-06-30 2024-07-12 キュー-ステート バイオサイエンシーズ, インコーポレイテッド 多重光学刺激および読出
US20250277028A1 (en) * 2022-04-25 2025-09-04 Jiksak Bioengineering, Inc. Targeting agent
WO2025084395A1 (ja) * 2023-10-19 2025-04-24 株式会社Jiksak Bioengineering 抗シナプトタグミン2抗体
WO2025089346A1 (ja) * 2023-10-25 2025-05-01 株式会社Jiksak Bioengineering 抗シナプトフィジン抗体

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2210911T3 (es) 1998-07-17 2004-07-01 Vertex Pharmaceuticals (San Diego) Llc Detector y dispositivo de seleccion para canales ionicos.
US6686193B2 (en) * 2000-07-10 2004-02-03 Vertex Pharmaceuticals, Inc. High throughput method and system for screening candidate compounds for activity against target ion channels
US7615356B2 (en) * 2000-07-10 2009-11-10 Vertex Pharmaceuticals (San Diego) Llc Ion channel assay methods
WO2003006103A2 (en) 2001-07-12 2003-01-23 Merck & Co., Inc. Electrical field stimulation of eukaryotic cells
US7221981B2 (en) * 2002-03-28 2007-05-22 Northstar Neuroscience, Inc. Electrode geometries for efficient neural stimulation
US20070274923A1 (en) 2005-01-11 2007-11-29 Philippe Brulet Non-invasive real-time in vivo bioluminescence imaging of local Ca2+ dynamics in living organisms

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WO2010027446A2 (en) 2010-03-11
US20120053084A1 (en) 2012-03-01
AU2009288657A1 (en) 2010-03-11
JP2012501646A (ja) 2012-01-26
JP2016019736A (ja) 2016-02-04
JP6106225B2 (ja) 2017-03-29
CA2744804C (en) 2019-02-05
US20180136198A1 (en) 2018-05-17
CA2744804A1 (en) 2010-03-11
WO2010027446A3 (en) 2010-05-20
EP2329275A2 (en) 2011-06-08
CN102203622A (zh) 2011-09-28
KR20110073494A (ko) 2011-06-29
WO2010027446A4 (en) 2010-08-05
AU2016202387A1 (en) 2016-05-05

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