JP5789513B2 - シナプス小胞サイクリングアッセイおよびシステム - Google Patents
シナプス小胞サイクリングアッセイおよびシステム Download PDFInfo
- Publication number
- JP5789513B2 JP5789513B2 JP2011526037A JP2011526037A JP5789513B2 JP 5789513 B2 JP5789513 B2 JP 5789513B2 JP 2011526037 A JP2011526037 A JP 2011526037A JP 2011526037 A JP2011526037 A JP 2011526037A JP 5789513 B2 JP5789513 B2 JP 5789513B2
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- Prior art keywords
- synaptic vesicle
- electrode
- synaptic
- wells
- platform
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5058—Neurological cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/10—Screening for compounds of potential therapeutic value involving cells
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Neurosurgery (AREA)
- Neurology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Toxicology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Electrochemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9436108P | 2008-09-04 | 2008-09-04 | |
| US61/094,361 | 2008-09-04 | ||
| PCT/US2009/004932 WO2010027446A2 (en) | 2008-09-04 | 2009-08-31 | Synaptic vesicle cycling assays and systems |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015151623A Division JP6106225B2 (ja) | 2008-09-04 | 2015-07-31 | シナプス小胞サイクリングアッセイおよびシステム |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012501646A JP2012501646A (ja) | 2012-01-26 |
| JP2012501646A5 JP2012501646A5 (enExample) | 2012-10-18 |
| JP5789513B2 true JP5789513B2 (ja) | 2015-10-07 |
Family
ID=41479300
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011526037A Expired - Fee Related JP5789513B2 (ja) | 2008-09-04 | 2009-08-31 | シナプス小胞サイクリングアッセイおよびシステム |
| JP2015151623A Expired - Fee Related JP6106225B2 (ja) | 2008-09-04 | 2015-07-31 | シナプス小胞サイクリングアッセイおよびシステム |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015151623A Expired - Fee Related JP6106225B2 (ja) | 2008-09-04 | 2015-07-31 | シナプス小胞サイクリングアッセイおよびシステム |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20120053084A1 (enExample) |
| EP (1) | EP2329275A2 (enExample) |
| JP (2) | JP5789513B2 (enExample) |
| KR (1) | KR20110073494A (enExample) |
| CN (1) | CN102203622A (enExample) |
| AU (2) | AU2009288657A1 (enExample) |
| CA (1) | CA2744804C (enExample) |
| WO (1) | WO2010027446A2 (enExample) |
Families Citing this family (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011511665A (ja) * | 2008-02-04 | 2011-04-14 | バイエル・ヘルスケア・エルエルシー | 半導体を素材とする分析対象物センサー及び方法 |
| US9217155B2 (en) | 2008-05-28 | 2015-12-22 | University Of Massachusetts | Isolation of novel AAV'S and uses thereof |
| US8734809B2 (en) | 2009-05-28 | 2014-05-27 | University Of Massachusetts | AAV's and uses thereof |
| US9057734B2 (en) | 2010-08-23 | 2015-06-16 | President And Fellows Of Harvard College | Optogenetic probes for measuring membrane potential |
| US9207237B2 (en) | 2010-08-23 | 2015-12-08 | President And Fellows Of Harvard College | Systems, methods, and workflows for optogenetics analysis |
| US9488637B2 (en) * | 2011-01-26 | 2016-11-08 | The Curators Of The University Of Missouri | Combination of single-cell electroporation and electrical recording using the same electrode |
| WO2012145624A2 (en) | 2011-04-21 | 2012-10-26 | University Of Massachusetts | Raav-based compositions and methods for treating alpha-1 anti-trypsin deficiencies |
| US20150004637A1 (en) * | 2011-11-23 | 2015-01-01 | President And Fellows Of Harvard College | Systems and methods for imaging at high spatial and/or temporal precision |
| US9696275B2 (en) | 2012-10-12 | 2017-07-04 | The Curators Of The University Of Missouri | Addressable electrode arrays in multiple fluidic compartments and uses thereof |
| WO2014065330A1 (ja) | 2012-10-25 | 2014-05-01 | 浜松ホトニクス株式会社 | 細胞観察装置、電気刺激装置、及び細胞観察方法 |
| WO2014065329A1 (ja) * | 2012-10-25 | 2014-05-01 | 浜松ホトニクス株式会社 | 細胞観察装置及び細胞観察方法 |
| JP6370544B2 (ja) * | 2013-11-11 | 2018-08-08 | 浜松ホトニクス株式会社 | 細胞観察装置及び細胞観察方法 |
| EP3095853B1 (en) | 2014-01-16 | 2019-01-30 | Hamamatsu Photonics K.K. | Cell observation device, electrostimulation device, and cell observation method |
| WO2015127128A2 (en) | 2014-02-19 | 2015-08-27 | University Of Massachusetts | Recombinant aavs having useful transcytosis properties |
| DK3119797T3 (da) | 2014-03-18 | 2021-03-15 | Univ Massachusetts | Raav-baserede sammensætninger og fremgangsmåder til behandling af amyotrofisk lateralsklerose |
| JP2017521044A (ja) * | 2014-04-22 | 2017-08-03 | キュー−ステート バイオサイエンシーズ, インコーポレイテッドQ−State Biosciences, Inc. | 疼痛及び感覚障害のための化合物の分析 |
| US10975391B2 (en) | 2014-04-25 | 2021-04-13 | University Of Massachusetts | Recombinant AAV vectors useful for reducing immunity against transgene products |
| WO2015195769A2 (en) | 2014-06-18 | 2015-12-23 | President And Fellows Of Harvard College | Optogenetic probes for measuring membrane potential |
| WO2016033501A1 (en) | 2014-08-28 | 2016-03-03 | Robert John Petcavich | Method of fabricating cell arrays and uses thereof |
| US10711270B2 (en) | 2014-10-03 | 2020-07-14 | University Of Massachusetts | High efficiency library-identified AAV vectors |
| US10370432B2 (en) | 2014-10-03 | 2019-08-06 | University Of Massachusetts | Heterologous targeting peptide grafted AAVS |
| RU2020140209A (ru) | 2014-10-21 | 2021-01-25 | Юниверсити Оф Массачусетс | Варианты рекомбинантных aav и их применения |
| US10077463B2 (en) | 2015-01-15 | 2018-09-18 | President And Fellows Of Harvard College | Optical selection of cells |
| EP3256170B1 (en) | 2015-02-13 | 2020-09-23 | University of Massachusetts | Compositions and methods for transient delivery of nucleases |
| US10048275B2 (en) | 2015-03-13 | 2018-08-14 | Q-State Biosciences, Inc. | Cardiotoxicity screening methods |
| US11020443B2 (en) | 2015-04-23 | 2021-06-01 | University Of Massachusetts | Modulation of AAV vector transgene expression |
| WO2016172008A1 (en) | 2015-04-24 | 2016-10-27 | University Of Massachusetts | Modified aav constructions and uses thereof |
| US11248212B2 (en) | 2015-06-30 | 2022-02-15 | StemoniX Inc. | Surface energy directed cell self assembly |
| US10760053B2 (en) | 2015-10-15 | 2020-09-01 | StemoniX Inc. | Method of manufacturing or differentiating mammalian pluripotent stem cells or progenitor cells using a hollow fiber bioreactor |
| US11426469B2 (en) | 2015-10-22 | 2022-08-30 | University Of Massachusetts | Prostate-targeting adeno-associated virus serotype vectors |
| CA3002982A1 (en) | 2015-10-22 | 2017-04-27 | University Of Massachusetts | Methods and compositions for treating metabolic imbalance in neurodegenerative disease |
| US11826433B2 (en) | 2016-02-02 | 2023-11-28 | University Of Massachusetts | Method to enhance the efficiency of systemic AAV gene delivery to the central nervous system |
| CA3012344A1 (en) | 2016-02-12 | 2017-08-17 | University Of Massachusetts | Anti-angiogenic mirna therapeutics for inhibiting corneal neovascularization |
| EP3225279B1 (en) * | 2016-03-31 | 2023-12-20 | Université de Rennes | Brain tissue stimulation apparatus and computer program |
| WO2017176929A1 (en) | 2016-04-05 | 2017-10-12 | University Of Massachusetts | Compositions and methods for selective inhibition of grainyhead-like protein expression |
| US11413356B2 (en) | 2016-04-15 | 2022-08-16 | University Of Massachusetts | Methods and compositions for treating metabolic imbalance |
| US11882815B2 (en) | 2016-06-15 | 2024-01-30 | University Of Massachusetts | Recombinant adeno-associated viruses for delivering gene editing molecules to embryonic cells |
| US10457940B2 (en) | 2016-09-22 | 2019-10-29 | University Of Massachusetts | AAV treatment of Huntington's disease |
| AU2017341849B2 (en) | 2016-10-13 | 2024-03-21 | University Of Massachusetts | AAV capsid designs |
| JP7327803B2 (ja) | 2017-05-09 | 2023-08-16 | ユニバーシティ オブ マサチューセッツ | 筋萎縮性側索硬化症(als)を処置する方法 |
| CN111448321A (zh) | 2017-09-22 | 2020-07-24 | 马萨诸塞大学 | Sod1双表达载体及其用途 |
| CA3096102A1 (en) | 2018-04-13 | 2019-10-17 | University Of Massachusetts | Bicistronic aav vectors encoding hexosaminidase alpha and beta-subunits and uses thereof |
| JP7065433B2 (ja) * | 2018-08-14 | 2022-05-12 | 日本電信電話株式会社 | 電極及びその製造方法、並びに積層体 |
| KR20210087462A (ko) | 2018-10-05 | 2021-07-12 | 유니버시티 오브 매사추세츠 | Gm1 및 gm2 강글리오시드증의 치료를 위한 raav 벡터 |
| SG11202111169YA (en) * | 2019-07-16 | 2021-11-29 | Meso Scale Technologies Llc | Assay apparatuses, methods and reagents |
| EP4271789A4 (en) * | 2020-12-31 | 2024-11-27 | Q-State Biosciences, Inc. | PLATE IMAGE PRODUCER |
| JP2024501848A (ja) | 2020-12-31 | 2024-01-16 | キュー-ステート バイオサイエンシーズ, インコーポレイテッド | 光遺伝学のためのイメージャの較正および正規化 |
| EP4271982A4 (en) * | 2020-12-31 | 2024-11-27 | Q-State Biosciences, Inc. | Optical plate controller and reader |
| EP4271978A4 (en) | 2020-12-31 | 2024-12-18 | Q-State Biosciences, Inc. | MICROSCOPE WITH SPATIAL IMAGING AND BEAM HOMOGENIZER |
| JP2024516303A (ja) * | 2021-05-04 | 2024-04-12 | キュー-ステート バイオサイエンシーズ, インコーポレイテッド | 薬物フィンガプリンティング |
| JP2024525493A (ja) | 2021-06-30 | 2024-07-12 | キュー-ステート バイオサイエンシーズ, インコーポレイテッド | 多重光学刺激および読出 |
| US20250277028A1 (en) * | 2022-04-25 | 2025-09-04 | Jiksak Bioengineering, Inc. | Targeting agent |
| WO2025084395A1 (ja) * | 2023-10-19 | 2025-04-24 | 株式会社Jiksak Bioengineering | 抗シナプトタグミン2抗体 |
| WO2025089346A1 (ja) * | 2023-10-25 | 2025-05-01 | 株式会社Jiksak Bioengineering | 抗シナプトフィジン抗体 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2210911T3 (es) | 1998-07-17 | 2004-07-01 | Vertex Pharmaceuticals (San Diego) Llc | Detector y dispositivo de seleccion para canales ionicos. |
| US6686193B2 (en) * | 2000-07-10 | 2004-02-03 | Vertex Pharmaceuticals, Inc. | High throughput method and system for screening candidate compounds for activity against target ion channels |
| US7615356B2 (en) * | 2000-07-10 | 2009-11-10 | Vertex Pharmaceuticals (San Diego) Llc | Ion channel assay methods |
| WO2003006103A2 (en) | 2001-07-12 | 2003-01-23 | Merck & Co., Inc. | Electrical field stimulation of eukaryotic cells |
| US7221981B2 (en) * | 2002-03-28 | 2007-05-22 | Northstar Neuroscience, Inc. | Electrode geometries for efficient neural stimulation |
| US20070274923A1 (en) | 2005-01-11 | 2007-11-29 | Philippe Brulet | Non-invasive real-time in vivo bioluminescence imaging of local Ca2+ dynamics in living organisms |
-
2009
- 2009-08-31 CN CN2009801440769A patent/CN102203622A/zh active Pending
- 2009-08-31 US US13/062,459 patent/US20120053084A1/en not_active Abandoned
- 2009-08-31 WO PCT/US2009/004932 patent/WO2010027446A2/en not_active Ceased
- 2009-08-31 JP JP2011526037A patent/JP5789513B2/ja not_active Expired - Fee Related
- 2009-08-31 CA CA2744804A patent/CA2744804C/en not_active Expired - Fee Related
- 2009-08-31 KR KR1020117007795A patent/KR20110073494A/ko not_active Ceased
- 2009-08-31 AU AU2009288657A patent/AU2009288657A1/en not_active Abandoned
- 2009-08-31 EP EP09789240A patent/EP2329275A2/en not_active Withdrawn
-
2015
- 2015-07-31 JP JP2015151623A patent/JP6106225B2/ja not_active Expired - Fee Related
-
2016
- 2016-04-15 AU AU2016202387A patent/AU2016202387A1/en not_active Abandoned
-
2018
- 2018-01-11 US US15/868,200 patent/US20180136198A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2010027446A2 (en) | 2010-03-11 |
| US20120053084A1 (en) | 2012-03-01 |
| AU2009288657A1 (en) | 2010-03-11 |
| JP2012501646A (ja) | 2012-01-26 |
| JP2016019736A (ja) | 2016-02-04 |
| JP6106225B2 (ja) | 2017-03-29 |
| CA2744804C (en) | 2019-02-05 |
| US20180136198A1 (en) | 2018-05-17 |
| CA2744804A1 (en) | 2010-03-11 |
| WO2010027446A3 (en) | 2010-05-20 |
| EP2329275A2 (en) | 2011-06-08 |
| CN102203622A (zh) | 2011-09-28 |
| KR20110073494A (ko) | 2011-06-29 |
| WO2010027446A4 (en) | 2010-08-05 |
| AU2016202387A1 (en) | 2016-05-05 |
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