CA2744804C - Synaptic vesicle cycling assays and systems - Google Patents

Synaptic vesicle cycling assays and systems Download PDF

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Publication number
CA2744804C
CA2744804C CA2744804A CA2744804A CA2744804C CA 2744804 C CA2744804 C CA 2744804C CA 2744804 A CA2744804 A CA 2744804A CA 2744804 A CA2744804 A CA 2744804A CA 2744804 C CA2744804 C CA 2744804C
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CA
Canada
Prior art keywords
synaptic vesicle
electrode
wells
platform
vesicle cycling
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Expired - Fee Related
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CA2744804A
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English (en)
French (fr)
Other versions
CA2744804A1 (en
Inventor
David J. Gerber
Jeffrey R. Cottrell
Timothy A. Ryan
Jonathan M. Levenson
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Galenea Corp
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Galenea Corp
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5058—Neurological cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Biophysics (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
  • Peptides Or Proteins (AREA)
CA2744804A 2008-09-04 2009-08-31 Synaptic vesicle cycling assays and systems Expired - Fee Related CA2744804C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9436108P 2008-09-04 2008-09-04
US61/094,361 2008-09-04
PCT/US2009/004932 WO2010027446A2 (en) 2008-09-04 2009-08-31 Synaptic vesicle cycling assays and systems

Publications (2)

Publication Number Publication Date
CA2744804A1 CA2744804A1 (en) 2010-03-11
CA2744804C true CA2744804C (en) 2019-02-05

Family

ID=41479300

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2744804A Expired - Fee Related CA2744804C (en) 2008-09-04 2009-08-31 Synaptic vesicle cycling assays and systems

Country Status (8)

Country Link
US (2) US20120053084A1 (enExample)
EP (1) EP2329275A2 (enExample)
JP (2) JP5789513B2 (enExample)
KR (1) KR20110073494A (enExample)
CN (1) CN102203622A (enExample)
AU (2) AU2009288657A1 (enExample)
CA (1) CA2744804C (enExample)
WO (1) WO2010027446A2 (enExample)

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US9696275B2 (en) 2012-10-12 2017-07-04 The Curators Of The University Of Missouri Addressable electrode arrays in multiple fluidic compartments and uses thereof
JP6346096B2 (ja) * 2012-10-25 2018-06-20 浜松ホトニクス株式会社 細胞観察装置及び細胞観察方法
WO2014065330A1 (ja) 2012-10-25 2014-05-01 浜松ホトニクス株式会社 細胞観察装置、電気刺激装置、及び細胞観察方法
JP6370544B2 (ja) * 2013-11-11 2018-08-08 浜松ホトニクス株式会社 細胞観察装置及び細胞観察方法
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WO2015127128A2 (en) 2014-02-19 2015-08-27 University Of Massachusetts Recombinant aavs having useful transcytosis properties
JP6756700B2 (ja) 2014-03-18 2020-09-16 ユニバーシティ オブ マサチューセッツ 筋萎縮性側索硬化症を処置するためのrAAVベースの組成物および方法
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EP3158338B1 (en) 2014-06-18 2020-03-11 President and Fellows of Harvard College Optogenetic probes for measuring membrane potential
WO2016033501A1 (en) 2014-08-28 2016-03-03 Robert John Petcavich Method of fabricating cell arrays and uses thereof
US10370432B2 (en) 2014-10-03 2019-08-06 University Of Massachusetts Heterologous targeting peptide grafted AAVS
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CA3011939A1 (en) 2016-02-02 2017-08-10 University Of Massachusetts Method to enhance the efficiency of systemic aav gene delivery to the central nervous system
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EP3225279B1 (en) * 2016-03-31 2023-12-20 Université de Rennes Brain tissue stimulation apparatus and computer program
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WO2019200286A1 (en) 2018-04-13 2019-10-17 University Of Massachusetts Bicistronic aav vectors encoding hexosaminidase alpha and beta-subunits and uses thereof
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Also Published As

Publication number Publication date
KR20110073494A (ko) 2011-06-29
WO2010027446A4 (en) 2010-08-05
AU2009288657A1 (en) 2010-03-11
JP5789513B2 (ja) 2015-10-07
US20120053084A1 (en) 2012-03-01
JP2016019736A (ja) 2016-02-04
US20180136198A1 (en) 2018-05-17
JP2012501646A (ja) 2012-01-26
CN102203622A (zh) 2011-09-28
CA2744804A1 (en) 2010-03-11
AU2016202387A1 (en) 2016-05-05
WO2010027446A3 (en) 2010-05-20
JP6106225B2 (ja) 2017-03-29
EP2329275A2 (en) 2011-06-08
WO2010027446A2 (en) 2010-03-11

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