JP5784266B2 - 規定細胞培養表面及び使用方法 - Google Patents

規定細胞培養表面及び使用方法 Download PDF

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JP5784266B2
JP5784266B2 JP2009174472A JP2009174472A JP5784266B2 JP 5784266 B2 JP5784266 B2 JP 5784266B2 JP 2009174472 A JP2009174472 A JP 2009174472A JP 2009174472 A JP2009174472 A JP 2009174472A JP 5784266 B2 JP5784266 B2 JP 5784266B2
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cell culture
cells
culture substrate
cell
extracellular matrix
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JP2010029186A5 (https=
JP2010029186A (ja
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サニヤル スパルナ
サニヤル スパルナ
サクセナ ディーパ
サクセナ ディーパ
ジューキ キアン スーザン
ジューキ キアン スーザン
アブラハム エリザベス
アブラハム エリザベス
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Corning Inc
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Corning Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
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    • C12N5/0602—Vertebrate cells
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    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
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    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
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    • C12N5/0602—Vertebrate cells
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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JP2009174472A 2008-07-25 2009-07-27 規定細胞培養表面及び使用方法 Active JP5784266B2 (ja)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US8357008P 2008-07-25 2008-07-25
US61/083,570 2008-07-25
US8504408P 2008-08-13 2008-08-13
US61/085,044 2008-08-13
US12/508,661 US8288513B2 (en) 2008-07-25 2009-07-24 Defined cell culturing surfaces and methods of use
US12/508,661 2009-07-24

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JP2010029186A JP2010029186A (ja) 2010-02-12
JP2010029186A5 JP2010029186A5 (https=) 2015-07-09
JP5784266B2 true JP5784266B2 (ja) 2015-09-24

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US (4) US8288513B2 (https=)
EP (3) EP2213728B1 (https=)
JP (1) JP5784266B2 (https=)
CN (2) CN101705186A (https=)
AU (1) AU2009203051A1 (https=)
CA (1) CA2674111A1 (https=)
PL (1) PL3323883T3 (https=)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8288513B2 (en) 2008-07-25 2012-10-16 Becton, Dickinson And Company Defined cell culturing surfaces and methods of use
WO2013170052A1 (en) 2012-05-09 2013-11-14 Sio2 Medical Products, Inc. Saccharide protective coating for pharmaceutical package
MX350703B (es) 2009-05-13 2017-09-14 Sio2 Medical Products Inc Metodo de gasificacion para inspeccionar una superficie revestida.
US9458536B2 (en) 2009-07-02 2016-10-04 Sio2 Medical Products, Inc. PECVD coating methods for capped syringes, cartridges and other articles
US11624115B2 (en) 2010-05-12 2023-04-11 Sio2 Medical Products, Inc. Syringe with PECVD lubrication
WO2011159684A2 (en) 2010-06-15 2011-12-22 Cellular Dynamics International, Inc. Generation of induced pluripotent stem cells from small volumes of peripheral blood
US9878101B2 (en) 2010-11-12 2018-01-30 Sio2 Medical Products, Inc. Cyclic olefin polymer vessels and vessel coating methods
US9272095B2 (en) 2011-04-01 2016-03-01 Sio2 Medical Products, Inc. Vessels, contact surfaces, and coating and inspection apparatus and methods
EP2695934B1 (en) 2011-04-05 2019-01-09 Hiroshima University Animal cell culture kit, method for culturing animal cells, method for selective culture of animal cells and cell differentiation method
EP2776603B1 (en) 2011-11-11 2019-03-06 SiO2 Medical Products, Inc. PASSIVATION, pH PROTECTIVE OR LUBRICITY COATING FOR PHARMACEUTICAL PACKAGE, COATING PROCESS AND APPARATUS
US11116695B2 (en) 2011-11-11 2021-09-14 Sio2 Medical Products, Inc. Blood sample collection tube
CN104271593B (zh) 2012-05-01 2018-01-16 富士胶片株式会社 多能干细胞的培养方法及该方法中使用的多肽
US20150297800A1 (en) 2012-07-03 2015-10-22 Sio2 Medical Products, Inc. SiOx BARRIER FOR PHARMACEUTICAL PACKAGE AND COATING PROCESS
US9193779B2 (en) * 2012-10-26 2015-11-24 Corning Incorporated Recombinant human fibronectin fragment for cell culture
CA2890066C (en) 2012-11-01 2021-11-09 Sio2 Medical Products, Inc. Coating inspection method
US9903782B2 (en) 2012-11-16 2018-02-27 Sio2 Medical Products, Inc. Method and apparatus for detecting rapid barrier coating integrity characteristics
US9764093B2 (en) 2012-11-30 2017-09-19 Sio2 Medical Products, Inc. Controlling the uniformity of PECVD deposition
JP6382830B2 (ja) 2012-11-30 2018-08-29 エスアイオーツー・メディカル・プロダクツ・インコーポレイテッド 医療シリンジ、カートリッジ等上でのｐｅｃｖｄ堆積の均一性制御
US9662450B2 (en) 2013-03-01 2017-05-30 Sio2 Medical Products, Inc. Plasma or CVD pre-treatment for lubricated pharmaceutical package, coating process and apparatus
US9937099B2 (en) 2013-03-11 2018-04-10 Sio2 Medical Products, Inc. Trilayer coated pharmaceutical packaging with low oxygen transmission rate
EP2971228B1 (en) 2013-03-11 2023-06-21 Si02 Medical Products, Inc. Coated packaging
US20160017490A1 (en) 2013-03-15 2016-01-21 Sio2 Medical Products, Inc. Coating method
WO2014201154A1 (en) * 2013-06-14 2014-12-18 Microstem, Inc. Matrix combinations support hematopoietic stem cell in vitro culturing
JP6169710B2 (ja) * 2013-09-10 2017-07-26 富士フイルム株式会社 多能性幹細胞の培養方法、培養用キット及び多能性幹細胞培養用培地
WO2015037623A1 (ja) 2013-09-10 2015-03-19 富士フイルム株式会社 多能性幹細胞の培養方法ならびにこれに用いる多能性幹細胞の培養用キットおよび培地
WO2015064661A1 (ja) 2013-10-31 2015-05-07 富士フイルム株式会社 ポリペプチド組成物およびこれを用いた多能性幹細胞の培養方法
GB201403558D0 (en) 2014-02-28 2014-04-16 P2I Ltd Coating
WO2015148471A1 (en) 2014-03-28 2015-10-01 Sio2 Medical Products, Inc. Antistatic coatings for plastic vessels
WO2015187241A1 (en) * 2014-06-02 2015-12-10 Sikorsky Aircraft Corporation Diagnosis of drive shaft disc couplings
US10625234B2 (en) 2014-08-28 2020-04-21 StemoniX Inc. Method of fabricating cell arrays and uses thereof
US9721081B2 (en) 2015-05-14 2017-08-01 Alclear, Llc Physical token-less security screening using biometrics
US11220669B2 (en) * 2015-06-05 2022-01-11 Amolifescience Co., Ltd. Defined three dimensional microenvironment for cell culture
WO2016198857A1 (en) 2015-06-09 2016-12-15 P2I Ltd Coatings
US11248212B2 (en) 2015-06-30 2022-02-15 StemoniX Inc. Surface energy directed cell self assembly
EP3337915B1 (en) 2015-08-18 2021-11-03 SiO2 Medical Products, Inc. Pharmaceutical and other packaging with low oxygen transmission rate
US10760053B2 (en) * 2015-10-15 2020-09-01 StemoniX Inc. Method of manufacturing or differentiating mammalian pluripotent stem cells or progenitor cells using a hollow fiber bioreactor
US20200392460A1 (en) * 2017-11-10 2020-12-17 Toppan Printing Co., Ltd. Artificial fat tissue and method for producing same, method for producing artificial skin and agent for fat cell culture
US10296048B1 (en) * 2018-03-14 2019-05-21 Htc Corporation Portable electronic device with dual displays and a hinge structure
WO2022076639A2 (en) * 2020-10-09 2022-04-14 Corning Incorporated Surface-modified cell culture substrates and methods of modifying cell culture substrates
JP7700843B2 (ja) * 2021-03-26 2025-07-01 東ソー株式会社 温度応答性高分子表面処理剤
US20240287430A1 (en) * 2021-06-02 2024-08-29 The Regents Of The University Of California Systems and Methods for Cellular Lumen Formation and Cellular Differentiation
CN114058504B (zh) * 2021-11-04 2023-10-20 南京艾尔普再生医学科技有限公司 一种拟胚体生产方式、容器以及装置

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989011500A1 (en) 1988-05-17 1989-11-30 Commonwealth Scientific And Industrial Research Or Hydrophilic non-swelling multilayer polymeric materials and process for their manufacture
JPH04252174A (ja) 1991-01-28 1992-09-08 Terumo Corp 組織培養用成形物およびその製造方法
US5246451A (en) 1991-04-30 1993-09-21 Medtronic, Inc. Vascular prosthesis and method
US5449383A (en) * 1992-03-18 1995-09-12 Chatelier; Ronald C. Cell growth substrates
JPH06303963A (ja) 1993-04-16 1994-11-01 Matsushita Electric Ind Co Ltd 細胞培養用基盤作製方法
JP3587438B2 (ja) * 1999-01-14 2004-11-10 住友ベークライト株式会社 培養細胞凍結用マルチウェルプレート
AR021240A1 (es) 1998-11-13 2002-07-03 Commw Scient Ind Res Org Peptidos acoplados
JP2005506839A (ja) * 2001-10-02 2005-03-10 ベクトン・ディキンソン・アンド・カンパニー 細胞外マトリックスおよび他の分子を使用する幹細胞の増殖および分化
WO2003035850A1 (en) 2001-10-26 2003-05-01 Celltran Limited Cell transfer substrate
JP2003325163A (ja) 2002-03-07 2003-11-18 Sumitomo Bakelite Co Ltd 細胞培養用フィルム、その製造方法、その細胞培養法及びその生物試験法
JP2005536214A (ja) * 2002-08-22 2005-12-02 セルトラン リミテッド 細胞培養表面
GB0225197D0 (en) * 2002-10-30 2002-12-11 Univ Sheffield Surface
WO2004094602A2 (en) 2003-04-16 2004-11-04 Emory University Combinatorial surface chip compositions for selection, differentiation and propagation of cells
GB0313569D0 (en) 2003-06-12 2003-07-16 Plasso Technology Ltd Method
JP2007536936A (ja) * 2004-05-14 2007-12-20 ベクトン・ディキンソン・アンド・カンパニー 幹細胞集団および使用方法
GB0606671D0 (en) 2006-04-03 2006-05-10 Plasso Technology Ltd Cell Culture
US8241908B2 (en) * 2006-05-16 2012-08-14 Becton, Dickinson And Company Extracellular matrix coated surface for culturing cells
US8642307B2 (en) 2006-05-25 2014-02-04 Nalge Nunc International Corporation Cell culture surface chemistries
CN101182646A (zh) * 2006-11-13 2008-05-21 北京有色金属研究总院 采用热交换法生长半球型晶体的装置及方法
US8288513B2 (en) * 2008-07-25 2012-10-16 Becton, Dickinson And Company Defined cell culturing surfaces and methods of use

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EP2147971B1 (en) 2018-04-11
US8728818B2 (en) 2014-05-20
US8916382B2 (en) 2014-12-23
EP2147971A2 (en) 2010-01-27
CN101705186A (zh) 2010-05-12
US8288513B2 (en) 2012-10-16
EP2147971A3 (en) 2010-04-28
EP3323883B1 (en) 2020-03-04
US20150024494A1 (en) 2015-01-22
US20130280803A1 (en) 2013-10-24
AU2009203051A1 (en) 2010-02-11
US9157059B2 (en) 2015-10-13
CA2674111A1 (en) 2010-01-25
EP2213728B1 (en) 2016-01-06
PL3323883T3 (pl) 2020-07-13
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CN106148189A (zh) 2016-11-23
JP2010029186A (ja) 2010-02-12
US20100021998A1 (en) 2010-01-28
US20140287506A1 (en) 2014-09-25
EP2213728A1 (en) 2010-08-04

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