JP5761996B2 - 合理的に設計された、非パリンドローム認識配列を有する単鎖メガヌクレアーゼ - Google Patents

合理的に設計された、非パリンドローム認識配列を有する単鎖メガヌクレアーゼ Download PDF

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JP5761996B2
JP5761996B2 JP2010532293A JP2010532293A JP5761996B2 JP 5761996 B2 JP5761996 B2 JP 5761996B2 JP 2010532293 A JP2010532293 A JP 2010532293A JP 2010532293 A JP2010532293 A JP 2010532293A JP 5761996 B2 JP5761996 B2 JP 5761996B2
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meganuclease
sequence
chain
laglidadg
nucleic acid
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JP2011501971A (ja
JP2011501971A5 (enExample
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スミス，ジェイムス，ジェファーソン
ジャンツ，デレック
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プレシジョン バイオサイエンシズ，インク．
プレシジョン バイオサイエンシズ，インク．
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
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    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
    • A—HUMAN NECESSITIES
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/04—Phosphoric diester hydrolases (3.1.4)

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  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
JP2010532293A 2007-10-31 2008-10-31 合理的に設計された、非パリンドローム認識配列を有する単鎖メガヌクレアーゼ Active JP5761996B2 (ja)

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US124707P 2007-10-31 2007-10-31
US61/001,247 2007-10-31
PCT/US2008/082072 WO2009059195A2 (en) 2007-10-31 2008-10-31 Rationally-designed single-chain meganucleases with non-palindromic recognition sequences

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JP2015115646A Division JP6050861B2 (ja) 2007-10-31 2015-06-08 合理的に設計された、非パリンドローム認識配列を有する単鎖メガヌクレアーゼ

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JP2011501971A JP2011501971A (ja) 2011-01-20
JP2011501971A5 JP2011501971A5 (enExample) 2011-12-22
JP5761996B2 true JP5761996B2 (ja) 2015-08-12

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JP2015115646A Active JP6050861B2 (ja) 2007-10-31 2015-06-08 合理的に設計された、非パリンドローム認識配列を有する単鎖メガヌクレアーゼ
JP2016193485A Pending JP2017051190A (ja) 2007-10-31 2016-09-30 合理的に設計された、非パリンドローム認識配列を有する単鎖メガヌクレアーゼ
JP2018219205A Pending JP2019062898A (ja) 2007-10-31 2018-11-22 合理的に設計された、非パリンドローム認識配列を有する単鎖メガヌクレアーゼ
JP2020196770A Pending JP2021052770A (ja) 2007-10-31 2020-11-27 合理的に設計された、非パリンドローム認識配列を有する単鎖メガヌクレアーゼ
JP2022187981A Pending JP2023029894A (ja) 2007-10-31 2022-11-25 合理的に設計された、非パリンドローム認識配列を有する単鎖メガヌクレアーゼ
JP2024187133A Pending JP2025028832A (ja) 2007-10-31 2024-10-24 合理的に設計された、非パリンドローム認識配列を有する単鎖メガヌクレアーゼ

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JP2016193485A Pending JP2017051190A (ja) 2007-10-31 2016-09-30 合理的に設計された、非パリンドローム認識配列を有する単鎖メガヌクレアーゼ
JP2018219205A Pending JP2019062898A (ja) 2007-10-31 2018-11-22 合理的に設計された、非パリンドローム認識配列を有する単鎖メガヌクレアーゼ
JP2020196770A Pending JP2021052770A (ja) 2007-10-31 2020-11-27 合理的に設計された、非パリンドローム認識配列を有する単鎖メガヌクレアーゼ
JP2022187981A Pending JP2023029894A (ja) 2007-10-31 2022-11-25 合理的に設計された、非パリンドローム認識配列を有する単鎖メガヌクレアーゼ
JP2024187133A Pending JP2025028832A (ja) 2007-10-31 2024-10-24 合理的に設計された、非パリンドローム認識配列を有する単鎖メガヌクレアーゼ

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US (10) US8445251B2 (enExample)
EP (4) EP3578646A3 (enExample)
JP (7) JP5761996B2 (enExample)
AU (1) AU2008318430A1 (enExample)
CA (1) CA2704383A1 (enExample)
DK (3) DK2660317T3 (enExample)
ES (3) ES2575412T3 (enExample)
WO (1) WO2009059195A2 (enExample)

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