JP5757885B2 - Ｐｃｒ用の試薬および方法 - Google Patents

Ｐｃｒ用の試薬および方法 Download PDF

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Publication number
JP5757885B2
JP5757885B2 JP2011554206A JP2011554206A JP5757885B2 JP 5757885 B2 JP5757885 B2 JP 5757885B2 JP 2011554206 A JP2011554206 A JP 2011554206A JP 2011554206 A JP2011554206 A JP 2011554206A JP 5757885 B2 JP5757885 B2 JP 5757885B2
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Japan
Prior art keywords
additive
primer
amplification
target
stranded
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JP2011554206A
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Japanese (ja)
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JP2012520080A (ja
JP2012520080A5 (enExample
Inventor
ワン，ローレンス，ジェイ．
ライス，ジョン
ライス，ニコラス
ジイア，ヤンウェイ
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Brandeis University
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Brandeis University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2011554206A 2009-03-12 2010-03-11 Ｐｃｒ用の試薬および方法 Expired - Fee Related JP5757885B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20256509P 2009-03-12 2009-03-12
US61/202,565 2009-03-12
PCT/US2010/027011 WO2010105074A1 (en) 2009-03-12 2010-03-11 Reagents and methods for pcr

Publications (3)

Publication Number Publication Date
JP2012520080A JP2012520080A (ja) 2012-09-06
JP2012520080A5 JP2012520080A5 (enExample) 2013-05-02
JP5757885B2 true JP5757885B2 (ja) 2015-08-05

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Family Applications (1)

Application Number Title Priority Date Filing Date
JP2011554206A Expired - Fee Related JP5757885B2 (ja) 2009-03-12 2010-03-11 Ｐｃｒ用の試薬および方法

Country Status (7)

Country Link
US (3) US9034605B2 (enExample)
EP (2) EP2406394B1 (enExample)
JP (1) JP5757885B2 (enExample)
CN (1) CN102421918B (enExample)
AU (1) AU2010224100B2 (enExample)
CA (1) CA2755207A1 (enExample)
WO (1) WO2010105074A1 (enExample)

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WO2009137059A1 (en) 2008-05-05 2009-11-12 Los Alamos National Security, Llc Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control
EP2406394B1 (en) 2009-03-12 2014-01-08 Brandeis University Reagents and methods for pcr
WO2012075231A1 (en) * 2010-12-03 2012-06-07 Brandeis University Methods and kits for detecting nucleic acid mutants in wild-type populations
EP2699698B9 (en) 2011-04-20 2020-07-15 Mesa Biotech, Inc. Oscillating amplification reaction for nucleic acids
JP2015508655A (ja) 2012-02-14 2015-03-23 コーネル ユニバーシティー 組み合わせたヌクレアーゼ反応、連結反応、およびポリメラーゼ反応を用いて核酸配列、発現、またはコピー変化を相対的に定量するための方法
EP2948564B1 (en) * 2013-01-23 2019-04-10 Brandeis University Reagents for improving pcr accuracy
KR102126031B1 (ko) * 2013-07-23 2020-07-08 삼성전자주식회사 유전적 변이를 함유하는 핵산 검출 방법
EP3666903B1 (en) 2013-08-12 2025-12-31 Bio-Rad Laboratories, Inc. AMPLIFICATION REPORTER GENE CONTAINING OLIGOMERS WITH BASIC PAIRINGS
EP3044311A4 (en) * 2013-09-13 2017-03-15 Brandeis University Modified polymerase compositions, methods and kits
EP3234186A1 (en) * 2014-12-19 2017-10-25 Brandeis University Mispriming prevention reagents
AU2016253147B2 (en) 2015-04-24 2021-07-22 Mesa Biotech, Inc. Fluidic test cassette
CA2994601C (en) * 2015-08-06 2020-08-25 F. Hoffmann-La Roche Ag Target enrichment by single probe primer extension
EP3400298B1 (en) 2016-01-08 2024-03-06 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
EP4219519A3 (en) * 2016-04-01 2023-09-13 Chromacode, Inc. Competitive probes for engineering signal generation
CN107541508A (zh) * 2016-06-24 2018-01-05 广州康昕瑞基因健康科技有限公司 模板‑引物核酸分子、聚合酶活性测定方法及试剂盒
CN108774639B (zh) * 2018-05-31 2023-05-30 澳門帝傑數碼基因有限公司 一种定向聚合的荧光探针pcr
EP3818170A4 (en) * 2018-07-02 2022-03-09 Swift Biosciences, Inc. Temperature controlled dna polymerase inhibitors
US20220307066A1 (en) * 2019-06-14 2022-09-29 University Of Washington An enzymatic assay to measure long-term adherence to pre exposure prophylaxis and antiretroviral therapy
US11597920B2 (en) 2019-06-25 2023-03-07 Bio-Rad Laboratories, Inc. Compositions and methods for enhancing reverse transcriptase activity and/or reducing the inhibition of reverse transcriptase
EP4107264A4 (en) * 2020-02-21 2024-06-05 Mission Bio, Inc. Enhanced detection of target nucleic acids by removal of dna-rna cross contamination
JP7209980B2 (ja) * 2020-12-11 2023-01-23 東洋紡株式会社 Ｄｎａポリメラーゼの５’→３’エキソヌクレアーゼ活性ドメインに特異的に結合する抗体
EP4367235A1 (en) * 2021-07-06 2024-05-15 Singular Genomics Systems, Inc. Compositions and methods for detecting genetic features
CN115747328A (zh) * 2022-08-19 2023-03-07 福建师范大学 一种提高单链dna产量和改善产物比例的方法

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US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
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US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
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WO1996041010A1 (en) 1995-06-07 1996-12-19 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands that bind to and inhibit dna polymerases
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
JP4567673B2 (ja) * 2003-04-01 2010-10-20 エラジェン バイオサイエンシズ インコーポレイテッド ポリメラーゼ阻害剤およびその使用方法
KR20150005720A (ko) * 2004-02-18 2015-01-14 크로모셀 코포레이션 신호 프로브를 이용하는 방법 및 물질
KR20070090158A (ko) * 2004-10-18 2007-09-05 브랜데이스 유니버시티 Ｐｃｒ 증폭에서 재현성을 개선시키고 미스프라이밍을감소시키기 위한 시약 및 방법
ES2668467T3 (es) 2004-10-18 2018-05-18 Brandeis University Métodos de amplificación LATE y secuenciación de un ácido nucleico
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EP2406394B1 (en) 2009-03-12 2014-01-08 Brandeis University Reagents and methods for pcr

Also Published As

Publication number Publication date
EP2406394A1 (en) 2012-01-18
CN102421918B (zh) 2016-01-20
CN102421918A (zh) 2012-04-18
US9034605B2 (en) 2015-05-19
US20180002739A1 (en) 2018-01-04
US20120088275A1 (en) 2012-04-12
CA2755207A1 (en) 2010-09-16
AU2010224100B2 (en) 2015-10-22
EP2703501A1 (en) 2014-03-05
EP2406394B1 (en) 2014-01-08
WO2010105074A1 (en) 2010-09-16
JP2012520080A (ja) 2012-09-06
US9758813B2 (en) 2017-09-12
AU2010224100A1 (en) 2011-10-27
US20150322503A1 (en) 2015-11-12

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