AU2010224100B2 - Reagents and methods for PCR - Google Patents

Reagents and methods for PCR Download PDF

Info

Publication number
AU2010224100B2
AU2010224100B2 AU2010224100A AU2010224100A AU2010224100B2 AU 2010224100 B2 AU2010224100 B2 AU 2010224100B2 AU 2010224100 A AU2010224100 A AU 2010224100A AU 2010224100 A AU2010224100 A AU 2010224100A AU 2010224100 B2 AU2010224100 B2 AU 2010224100B2
Authority
AU
Australia
Prior art keywords
additive
primer
amplification
dabcyl
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2010224100A
Other languages
English (en)
Other versions
AU2010224100A1 (en
Inventor
Yanwei Jia
John Rice
Nicholas Rice
Lawrence J. Wangh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brandeis University
Original Assignee
Brandeis University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brandeis University filed Critical Brandeis University
Publication of AU2010224100A1 publication Critical patent/AU2010224100A1/en
Application granted granted Critical
Publication of AU2010224100B2 publication Critical patent/AU2010224100B2/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2010224100A 2009-03-12 2010-03-11 Reagents and methods for PCR Ceased AU2010224100B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20256509P 2009-03-12 2009-03-12
US61/202,565 2009-03-12
PCT/US2010/027011 WO2010105074A1 (en) 2009-03-12 2010-03-11 Reagents and methods for pcr

Publications (2)

Publication Number Publication Date
AU2010224100A1 AU2010224100A1 (en) 2011-10-27
AU2010224100B2 true AU2010224100B2 (en) 2015-10-22

Family

ID=42244601

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2010224100A Ceased AU2010224100B2 (en) 2009-03-12 2010-03-11 Reagents and methods for PCR

Country Status (7)

Country Link
US (3) US9034605B2 (enExample)
EP (2) EP2703501A1 (enExample)
JP (1) JP5757885B2 (enExample)
CN (1) CN102421918B (enExample)
AU (1) AU2010224100B2 (enExample)
CA (1) CA2755207A1 (enExample)
WO (1) WO2010105074A1 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8980561B1 (en) 2006-08-22 2015-03-17 Los Alamos National Security, Llc. Nucleic acid detection system and method for detecting influenza
US20090047673A1 (en) 2006-08-22 2009-02-19 Cary Robert B Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
CA2723536C (en) 2008-05-05 2017-06-13 Los Alamos National Security, Llc Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control
US9034605B2 (en) 2009-03-12 2015-05-19 Brandeis University Reagents and methods for PCR
US9169514B2 (en) * 2010-12-03 2015-10-27 Brandeis University Detecting nucleic acid variations within populations of genomes
CN103649333B (zh) 2011-04-20 2017-02-15 梅撒生物科技公司 用于核酸检测和鉴定的集成装置
CN104204228A (zh) * 2012-02-14 2014-12-10 康奈尔大学 使用组合的核酸酶、连接和聚合酶反应用于核酸序列的相对定量、表达或拷贝变化的方法
WO2014116860A1 (en) * 2013-01-23 2014-07-31 Brandeis University Reagents for improving pcr accuracy
KR102126031B1 (ko) * 2013-07-23 2020-07-08 삼성전자주식회사 유전적 변이를 함유하는 핵산 검출 방법
WO2015023677A1 (en) 2013-08-12 2015-02-19 Bio-Rad Laboratories, Inc. Amplification reporter with base-pairing oligomers
WO2015038869A1 (en) * 2013-09-13 2015-03-19 Brandeis University Modified polymerase compositions, methods and kits
US10240178B2 (en) 2014-12-19 2019-03-26 Brandeis University Mispriming prevention reagents
AU2016253147B2 (en) 2015-04-24 2021-07-22 Mesa Biotech, Inc. Fluidic test cassette
CN107922970B (zh) * 2015-08-06 2021-09-28 豪夫迈·罗氏有限公司 通过单探针引物延伸的靶标富集
EP3400298B1 (en) 2016-01-08 2024-03-06 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
EP3436468B1 (en) * 2016-04-01 2023-03-22 Chromacode, Inc. Competitive probes for engineering signal generation
CN107541508A (zh) * 2016-06-24 2018-01-05 广州康昕瑞基因健康科技有限公司 模板‑引物核酸分子、聚合酶活性测定方法及试剂盒
CN108774639B (zh) * 2018-05-31 2023-05-30 澳門帝傑數碼基因有限公司 一种定向聚合的荧光探针pcr
EP3818170A4 (en) * 2018-07-02 2022-03-09 Swift Biosciences, Inc. Temperature controlled dna polymerase inhibitors
US20220307066A1 (en) * 2019-06-14 2022-09-29 University Of Washington An enzymatic assay to measure long-term adherence to pre exposure prophylaxis and antiretroviral therapy
CN119798354A (zh) * 2019-06-25 2025-04-11 生物辐射实验室股份有限公司 用于增强逆转录酶活性和/或减少逆转录酶抑制的组合物和方法
WO2021168384A1 (en) * 2020-02-21 2021-08-26 Mission Bio, Inc. Enhanced detection of target nucleic acids by removal of dna-rna cross contamination
JP7209980B2 (ja) * 2020-12-11 2023-01-23 東洋紡株式会社 Ｄｎａポリメラーゼの５’→３’エキソヌクレアーゼ活性ドメインに特異的に結合する抗体
WO2023283090A1 (en) * 2021-07-06 2023-01-12 Singular Genomics Systems, Inc. Compositions and methods for detecting genetic features
CN115747328A (zh) * 2022-08-19 2023-03-07 福建师范大学 一种提高单链dna产量和改善产物比例的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006044994A2 (en) * 2004-10-18 2006-04-27 Brandeis University Primers, probes and methods for nucleic acid amplification

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US20050242506A1 (en) 1994-07-22 2005-11-03 Shuffle Master, Inc. Poker game variation with variable size wagers and play against a pay table
CA2223078C (en) 1995-06-07 2008-11-25 Gilead Sciences, Inc. Nucleic acid ligands that bind to and inhibit dna polymerases
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
WO2004090153A2 (en) * 2003-04-01 2004-10-21 Eragen Biosciences,Inc. Polymerase inhibitor and method of using same
US8916503B2 (en) * 2004-02-18 2014-12-23 Chromocell Corporation Methods and materials using signaling probes
NZ554701A (en) * 2004-10-18 2010-03-26 Univ Brandeis Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification
AU2006269282B2 (en) 2005-07-07 2013-10-24 Quanta Biosciences, Inc. Compositions and methods for increasing amplification efficiency
DE602006008150D1 (de) * 2006-10-12 2009-09-10 Bio Rad Pasteur Doppelsträngige Sonden zur Fluoreszenzdetektion von Nukleinsäuren
BRPI0719995A2 (pt) * 2006-12-14 2014-03-18 Novartis Ag Composições e métodos para tratar distúrbios musculares & cardiovasculares
US9034605B2 (en) 2009-03-12 2015-05-19 Brandeis University Reagents and methods for PCR

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006044994A2 (en) * 2004-10-18 2006-04-27 Brandeis University Primers, probes and methods for nucleic acid amplification

Also Published As

Publication number Publication date
AU2010224100A1 (en) 2011-10-27
CN102421918B (zh) 2016-01-20
US9034605B2 (en) 2015-05-19
EP2406394B1 (en) 2014-01-08
JP2012520080A (ja) 2012-09-06
EP2406394A1 (en) 2012-01-18
CA2755207A1 (en) 2010-09-16
JP5757885B2 (ja) 2015-08-05
US20120088275A1 (en) 2012-04-12
EP2703501A1 (en) 2014-03-05
CN102421918A (zh) 2012-04-18
US20180002739A1 (en) 2018-01-04
WO2010105074A1 (en) 2010-09-16
US20150322503A1 (en) 2015-11-12
US9758813B2 (en) 2017-09-12

Similar Documents

Publication Publication Date Title
US9758813B2 (en) Reagents and methods of PCR
AU2007275762B2 (en) Specialized oligonucleotides and their use in nucleic acid amplification and detection
US20090226973A1 (en) Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification
CN103459612A (zh) 用于检测野生型群体中的核酸突变体的方法和试剂盒
US20200032310A1 (en) Mispriming prevention reagents
CN106566855B (zh) 使用可逆修饰寡核苷酸扩增核酸
Liu et al. Invading stacking primer: A trigger for high-efficiency isothermal amplification reaction with superior selectivity for detecting microRNA variants
US11840728B2 (en) Inhibition of nucleic acid polymerases by endonuclease V-cleavable oligonucleotide ligands
EP2841598B1 (en) Method for competitive allele-specific cdna synthesis and differential amplification of the cdna products
JP2021514637A (ja) 特異性を改善した核酸を増幅する方法
CA3220607A1 (en) Methods and compositions related to cooperative primers and reverse transcription
JP2008528021A (ja) 生化学試薬及びその使用

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired