CA2755207A1 - Reagents and methods for pcr - Google Patents

Reagents and methods for pcr Download PDF

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Publication number
CA2755207A1
CA2755207A1 CA2755207A CA2755207A CA2755207A1 CA 2755207 A1 CA2755207 A1 CA 2755207A1 CA 2755207 A CA2755207 A CA 2755207A CA 2755207 A CA2755207 A CA 2755207A CA 2755207 A1 CA2755207 A1 CA 2755207A1
Authority
CA
Canada
Prior art keywords
additive
primer
amplification
stranded
double
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2755207A
Other languages
English (en)
French (fr)
Inventor
Lawrence J. Wangh
John Rice
Nicholas Rice
Yanwei Jia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brandeis University
Original Assignee
Brandeis University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brandeis University filed Critical Brandeis University
Publication of CA2755207A1 publication Critical patent/CA2755207A1/en
Abandoned legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2755207A 2009-03-12 2010-03-11 Reagents and methods for pcr Abandoned CA2755207A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20256509P 2009-03-12 2009-03-12
US61/202,565 2009-03-12
PCT/US2010/027011 WO2010105074A1 (en) 2009-03-12 2010-03-11 Reagents and methods for pcr

Publications (1)

Publication Number Publication Date
CA2755207A1 true CA2755207A1 (en) 2010-09-16

Family

ID=42244601

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2755207A Abandoned CA2755207A1 (en) 2009-03-12 2010-03-11 Reagents and methods for pcr

Country Status (7)

Country Link
US (3) US9034605B2 (enExample)
EP (2) EP2406394B1 (enExample)
JP (1) JP5757885B2 (enExample)
CN (1) CN102421918B (enExample)
AU (1) AU2010224100B2 (enExample)
CA (1) CA2755207A1 (enExample)
WO (1) WO2010105074A1 (enExample)

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WO2008105814A2 (en) 2006-08-22 2008-09-04 Los Alamos National Security, Llc Miniturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
US8980561B1 (en) 2006-08-22 2015-03-17 Los Alamos National Security, Llc. Nucleic acid detection system and method for detecting influenza
EP2279403B1 (en) 2008-05-05 2016-03-16 Los Alamos National Security, LLC Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control
EP2406394B1 (en) 2009-03-12 2014-01-08 Brandeis University Reagents and methods for pcr
US9169514B2 (en) 2010-12-03 2015-10-27 Brandeis University Detecting nucleic acid variations within populations of genomes
ES2583135T3 (es) 2011-04-20 2016-09-19 Mesa Biotech, Inc. Dispositivo integrado para la detección e identificación de ácidos nucleicos
JP2015508655A (ja) 2012-02-14 2015-03-23 コーネル ユニバーシティー 組み合わせたヌクレアーゼ反応、連結反応、およびポリメラーゼ反応を用いて核酸配列、発現、またはコピー変化を相対的に定量するための方法
EP2948564B1 (en) * 2013-01-23 2019-04-10 Brandeis University Reagents for improving pcr accuracy
KR102126031B1 (ko) * 2013-07-23 2020-07-08 삼성전자주식회사 유전적 변이를 함유하는 핵산 검출 방법
CN105378160B (zh) * 2013-08-12 2018-04-17 伯乐生命医学产品有限公司 具有碱基配对的寡聚体的扩增报告子
US20160222434A1 (en) * 2013-09-13 2016-08-04 Brandeis University Modified Polymerase Compositions, Methods and Kits
CA2971200A1 (en) 2014-12-19 2016-06-23 Brandeis University Mispriming prevention reagents
WO2016172724A1 (en) 2015-04-24 2016-10-27 Mesa Biotech, Inc. Fluidic test cassette
JP6847496B2 (ja) * 2015-08-06 2021-03-24 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 単一プローブプライマー伸長による標的濃縮
CN108431223A (zh) 2016-01-08 2018-08-21 生物辐射实验室股份有限公司 每液滴分辨率下的多个珠
CN109601008B (zh) * 2016-04-01 2023-02-28 克罗玛科德公司 用于工程化信号产生的竞争性探针
CN107541508A (zh) * 2016-06-24 2018-01-05 广州康昕瑞基因健康科技有限公司 模板‑引物核酸分子、聚合酶活性测定方法及试剂盒
CN108774639B (zh) * 2018-05-31 2023-05-30 澳門帝傑數碼基因有限公司 一种定向聚合的荧光探针pcr
WO2020010124A2 (en) * 2018-07-02 2020-01-09 Swift Biosciences, Inc. Temperature controlled dna polymerase inhibitors
US20220307066A1 (en) * 2019-06-14 2022-09-29 University Of Washington An enzymatic assay to measure long-term adherence to pre exposure prophylaxis and antiretroviral therapy
CN114341150B (zh) * 2019-06-25 2025-01-14 生物辐射实验室股份有限公司 用于增强逆转录酶活性和/或减少逆转录酶抑制的组合物和方法
WO2021168384A1 (en) * 2020-02-21 2021-08-26 Mission Bio, Inc. Enhanced detection of target nucleic acids by removal of dna-rna cross contamination
JP7209980B2 (ja) * 2020-12-11 2023-01-23 東洋紡株式会社 Dnaポリメラーゼの5’→3’エキソヌクレアーゼ活性ドメインに特異的に結合する抗体
CN115747328A (zh) * 2022-08-19 2023-03-07 福建师范大学 一种提高单链dna产量和改善产物比例的方法

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US20050242506A1 (en) 1994-07-22 2005-11-03 Shuffle Master, Inc. Poker game variation with variable size wagers and play against a pay table
ATE494394T1 (de) 1995-06-07 2011-01-15 Gilead Sciences Inc Nukleinsäureliganden, die an dna-polymerasen binden und diese inhibieren
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
CA2252048C (en) 1996-04-12 2008-03-11 The Public Health Research Institute Of The City Of New York, Inc. Detection probes, kits and assays
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
CA2521127C (en) * 2003-04-01 2014-05-27 Eragen Biosciences, Inc. Polymerase inhibitor and method of using same
JP2007522818A (ja) * 2004-02-18 2007-08-16 クロモセル コーポレイション シグナリングプローブを使用する方法および材料
NZ554701A (en) * 2004-10-18 2010-03-26 Univ Brandeis Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification
RU2460804C2 (ru) * 2004-10-18 2012-09-10 Брандейс Юнивесити Способ гомогенной детекции по меньшей мере одного продукта одноцепочечной амплификации
CA2614444C (en) * 2005-07-07 2015-12-08 Quanta Biosciences, Inc. Compositions and methods for increasing amplification efficiency
EP1911852B1 (en) * 2006-10-12 2009-07-29 Bio-Rad Pasteur Double-stranded probes for the fluorescent detection of nucleic acids
EA200900782A1 (ru) * 2006-12-14 2009-12-30 Новартис Аг Композиции и способы, предназначенные для лечения мышечных и сердечно-сосудистых нарушений
EP2406394B1 (en) 2009-03-12 2014-01-08 Brandeis University Reagents and methods for pcr

Also Published As

Publication number Publication date
US20120088275A1 (en) 2012-04-12
EP2406394A1 (en) 2012-01-18
WO2010105074A1 (en) 2010-09-16
AU2010224100A1 (en) 2011-10-27
CN102421918A (zh) 2012-04-18
US9034605B2 (en) 2015-05-19
JP2012520080A (ja) 2012-09-06
EP2406394B1 (en) 2014-01-08
US9758813B2 (en) 2017-09-12
CN102421918B (zh) 2016-01-20
EP2703501A1 (en) 2014-03-05
JP5757885B2 (ja) 2015-08-05
US20180002739A1 (en) 2018-01-04
AU2010224100B2 (en) 2015-10-22
US20150322503A1 (en) 2015-11-12

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20150311

FZDE Dead

Effective date: 20170313