CN102421918B - 用于pcr的试剂和方法 - Google Patents

用于pcr的试剂和方法 Download PDF

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Publication number
CN102421918B
CN102421918B CN201080020170.6A CN201080020170A CN102421918B CN 102421918 B CN102421918 B CN 102421918B CN 201080020170 A CN201080020170 A CN 201080020170A CN 102421918 B CN102421918 B CN 102421918B
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China
Prior art keywords
amplification
additive
primer
double
pcr
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Chinese (zh)
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CN102421918A (zh
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L·J·万
J·赖斯
N·赖斯
贾艳伟
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Brandeis University
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Brandeis University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201080020170.6A 2009-03-12 2010-03-11 用于pcr的试剂和方法 Expired - Fee Related CN102421918B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20256509P 2009-03-12 2009-03-12
US61/202565 2009-03-12
PCT/US2010/027011 WO2010105074A1 (en) 2009-03-12 2010-03-11 Reagents and methods for pcr

Publications (2)

Publication Number Publication Date
CN102421918A CN102421918A (zh) 2012-04-18
CN102421918B true CN102421918B (zh) 2016-01-20

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US (3) US9034605B2 (enExample)
EP (2) EP2703501A1 (enExample)
JP (1) JP5757885B2 (enExample)
CN (1) CN102421918B (enExample)
AU (1) AU2010224100B2 (enExample)
CA (1) CA2755207A1 (enExample)
WO (1) WO2010105074A1 (enExample)

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CA2723536C (en) 2008-05-05 2017-06-13 Los Alamos National Security, Llc Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control
EP2703501A1 (en) 2009-03-12 2014-03-05 Brandeis University Reagents and Methods for PCR
EP2646576B1 (en) 2010-12-03 2015-10-28 Brandeis University Methods and kits for detecting nucleic acid mutants in wild-type populations
CN103649333B (zh) 2011-04-20 2017-02-15 梅撒生物科技公司 用于核酸检测和鉴定的集成装置
US9598728B2 (en) * 2012-02-14 2017-03-21 Cornell University Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions
EP2948564B1 (en) * 2013-01-23 2019-04-10 Brandeis University Reagents for improving pcr accuracy
KR102126031B1 (ko) * 2013-07-23 2020-07-08 삼성전자주식회사 유전적 변이를 함유하는 핵산 검출 방법
CN105378160B (zh) * 2013-08-12 2018-04-17 伯乐生命医学产品有限公司 具有碱基配对的寡聚体的扩增报告子
EP3044311A4 (en) * 2013-09-13 2017-03-15 Brandeis University Modified polymerase compositions, methods and kits
CA2971200A1 (en) * 2014-12-19 2016-06-23 Brandeis University Mispriming prevention reagents
WO2016172724A1 (en) 2015-04-24 2016-10-27 Mesa Biotech, Inc. Fluidic test cassette
CA2994601C (en) * 2015-08-06 2020-08-25 F. Hoffmann-La Roche Ag Target enrichment by single probe primer extension
CN108431223A (zh) 2016-01-08 2018-08-21 生物辐射实验室股份有限公司 每液滴分辨率下的多个珠
WO2017173035A1 (en) * 2016-04-01 2017-10-05 Chromacode Inc. Competitive probes for engineering signal generation
CN107541508A (zh) * 2016-06-24 2018-01-05 广州康昕瑞基因健康科技有限公司 模板‑引物核酸分子、聚合酶活性测定方法及试剂盒
CN108774639B (zh) * 2018-05-31 2023-05-30 澳門帝傑數碼基因有限公司 一种定向聚合的荧光探针pcr
EP3818170A4 (en) * 2018-07-02 2022-03-09 Swift Biosciences, Inc. Temperature controlled dna polymerase inhibitors
WO2020252399A1 (en) * 2019-06-14 2020-12-17 University Of Washington An enzymatic assay to measure long-term adherence to pre exposure prophylaxis and antiretroviral therapy
CN119798354A (zh) 2019-06-25 2025-04-11 生物辐射实验室股份有限公司 用于增强逆转录酶活性和/或减少逆转录酶抑制的组合物和方法
US20230101896A1 (en) * 2020-02-21 2023-03-30 Mission Bio, Inc. Enhanced Detection of Target Nucleic Acids by Removal of DNA-RNA Cross Contamination
JP7209980B2 (ja) * 2020-12-11 2023-01-23 東洋紡株式会社 Ｄｎａポリメラーゼの５’→３’エキソヌクレアーゼ活性ドメインに特異的に結合する抗体
CN115747328A (zh) * 2022-08-19 2023-03-07 福建师范大学 一种提高单链dna产量和改善产物比例的方法

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WO2004090153A2 (en) * 2003-04-01 2004-10-21 Eragen Biosciences,Inc. Polymerase inhibitor and method of using same
WO2006044994A2 (en) * 2004-10-18 2006-04-27 Brandeis University Primers, probes and methods for nucleic acid amplification
WO2007008728A2 (en) * 2005-07-07 2007-01-18 Quanta Biosciences, Inc. Compositions and methods for increasing amplification efficiency

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WO2006044994A2 (en) * 2004-10-18 2006-04-27 Brandeis University Primers, probes and methods for nucleic acid amplification
WO2007008728A2 (en) * 2005-07-07 2007-01-18 Quanta Biosciences, Inc. Compositions and methods for increasing amplification efficiency

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US20150322503A1 (en) 2015-11-12
EP2406394B1 (en) 2014-01-08
EP2703501A1 (en) 2014-03-05
AU2010224100A1 (en) 2011-10-27
US20180002739A1 (en) 2018-01-04
US9758813B2 (en) 2017-09-12
JP2012520080A (ja) 2012-09-06
JP5757885B2 (ja) 2015-08-05
CA2755207A1 (en) 2010-09-16
US20120088275A1 (en) 2012-04-12
EP2406394A1 (en) 2012-01-18
WO2010105074A1 (en) 2010-09-16
US9034605B2 (en) 2015-05-19
CN102421918A (zh) 2012-04-18
AU2010224100B2 (en) 2015-10-22

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