JP5624044B2 - 改善されたアレル−特異的増幅 - Google Patents

改善されたアレル−特異的増幅 Download PDF

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JP5624044B2
JP5624044B2 JP2011531404A JP2011531404A JP5624044B2 JP 5624044 B2 JP5624044 B2 JP 5624044B2 JP 2011531404 A JP2011531404 A JP 2011531404A JP 2011531404 A JP2011531404 A JP 2011531404A JP 5624044 B2 JP5624044 B2 JP 5624044B2
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oligonucleotide
target sequence
nucleotide
variant
complementary
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Japanese (ja)
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JP2012505641A5 (OSRAM
JP2012505641A (ja
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ゴードン ウィル，スティーブン
ゴードン ウィル，スティーブン
ニュートン，ニコラス
ツァン，アリソン
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2011531404A 2008-10-20 2009-10-17 改善されたアレル−特異的増幅 Active JP5624044B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10678308P 2008-10-20 2008-10-20
US61/106,783 2008-10-20
PCT/EP2009/007463 WO2010046067A1 (en) 2008-10-20 2009-10-17 Improved allele-specific amplification

Publications (3)

Publication Number Publication Date
JP2012505641A JP2012505641A (ja) 2012-03-08
JP2012505641A5 JP2012505641A5 (OSRAM) 2012-05-24
JP5624044B2 true JP5624044B2 (ja) 2014-11-12

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JP2011531404A Active JP5624044B2 (ja) 2008-10-20 2009-10-17 改善されたアレル−特異的増幅

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US (2) US8586299B2 (OSRAM)
EP (1) EP2337865B1 (OSRAM)
JP (1) JP5624044B2 (OSRAM)
CN (2) CN105200097A (OSRAM)
CA (1) CA2740913C (OSRAM)
ES (1) ES2527653T3 (OSRAM)
HK (1) HK1219508A1 (OSRAM)
WO (1) WO2010046067A1 (OSRAM)

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WO2010046067A1 (en) 2008-10-20 2010-04-29 Roche Diagnostics Gmbh Improved allele-specific amplification
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
CN103459612A (zh) * 2010-12-03 2013-12-18 布兰代斯大学 用于检测野生型群体中的核酸突变体的方法和试剂盒
US20120164641A1 (en) 2010-12-22 2012-06-28 Roche Molecular Systems, Inc. Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene
CA2847943C (en) * 2011-09-23 2017-08-29 F.Hoffmann-La Roche Ag Use of g-clamp for improved allele-specific pcr
JP2014532407A (ja) * 2011-10-24 2014-12-08 トローバジーン インコーポレイテッド がんにおけるｂｒａｆ変異を検出する方法
US9738935B2 (en) 2011-11-10 2017-08-22 Roche Molecular Systems, Inc. Complex mutations in the epidermal growth factor receptor kinase domain
JP2014533508A (ja) * 2011-11-17 2014-12-15 リーアニクス・インコーポレイテッドＲｈｅｏｎｉｘ， Ｉｎｃ． 選択的分子分析のためのシステムおよび方法
WO2013123031A2 (en) * 2012-02-15 2013-08-22 Veridex, Llc Highly sensitive method for detecting low frequency mutations
US20140341884A1 (en) 2012-12-04 2014-11-20 Roche Molecular Systems, Inc. Novel Complex Mutations in the Epidermal Growth Factor Receptor Kinase Domain
US9382581B2 (en) * 2012-12-13 2016-07-05 Roche Molecular Systems, Inc. Primers with modified phosphate and base in allele-specific PCR
EP2971075B1 (en) * 2013-03-13 2018-08-15 Roche Diagnostics GmbH Methods and compositions for detecting mutations in the human pi3kca (pik3ca) gene
AU2014306867B2 (en) 2013-08-12 2017-10-26 Genentech, Inc. Compositions and method for treating complement-associated conditions
US10260102B2 (en) 2013-10-09 2019-04-16 Roche Molecular Systems, Inc. Methods and compositions for detecting mutation in the human EZH2 gene
TWI785472B (zh) 2014-02-08 2022-12-01 美商建南德克公司 治療阿茲海默症之方法
CN103911445B (zh) * 2014-03-20 2015-08-12 上海科亦生物科技有限公司 一种as-pcr引物设计方法、基因多态性检测方法及试剂盒
JP2017515484A (ja) * 2014-05-19 2017-06-15 ウィリアム マーシュ ライス ユニバーシティＷｉｌｌｉａｍ Ｍａｒｓｈ Ｒｉｃｅ Ｕｎｉｖｅｒｓｉｔｙ 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅
EP3204510B1 (en) 2014-10-09 2018-07-25 Roche Diagnostics GmbH Mutations in the epidermal growth factor receptor kinase domain
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
CN104845967B (zh) * 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
CN105400900A (zh) * 2015-12-29 2016-03-16 杭州迪安生物技术有限公司 焦磷酸测序技术检测braf基因v600e微量突变试剂盒及其应用
US20170349953A1 (en) 2016-06-01 2017-12-07 Roche Sequencing Solutions, Inc. Novel mutations in anaplastic lymphoma kinase predicting response to alk inhibitor therapy in lung cancer patients
US11384376B2 (en) * 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
CN110964818A (zh) * 2019-11-28 2020-04-07 重庆浦洛通基因医学研究院有限公司 一种人类braf基因v600e突变的检测试剂盒及检测方法
CN113215227A (zh) * 2021-02-04 2021-08-06 郑州华沃生物科技有限公司 一种人维生素d受体基因分型检测用的引物和探针

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DE3529478A1 (de) * 1985-08-16 1987-02-19 Boehringer Mannheim Gmbh 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung
IE61148B1 (en) * 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
US5521301A (en) * 1988-12-12 1996-05-28 City Of Hope Genotyping of multiple allele systems
US5639611A (en) * 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
US5137806A (en) * 1989-12-11 1992-08-11 Board Of Regents, The University Of Texas System Methods and compositions for the detection of sequences in selected DNA molecules
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DK0866071T3 (da) * 1997-03-20 2005-01-17 Hoffmann La Roche Modificerede primere
EP0974672B1 (en) * 1998-07-21 2003-04-09 Keygene N.V. Improved primers for AFLP amplification
US6200757B1 (en) * 1999-01-19 2001-03-13 Dade Behring Inc. Method for controlling the extension of an oligonucleotide
KR100809949B1 (ko) * 2001-02-15 2008-03-06 다카라 바이오 가부시키가이샤 염기 치환의 검출 방법
JP2005524383A (ja) * 2001-08-28 2005-08-18 ファルマシア・アンド・アップジョン・カンパニー・エルエルシー 統合失調症の診断に用いる一塩基多型
US7408051B2 (en) * 2004-04-14 2008-08-05 Applera Corporation Modified oligonucleotides and applications thereof
EP2021488B1 (en) * 2006-04-28 2018-09-12 Igor Kutyavin Use of base-modified deoxynucleoside triphosphates to improve nucleic acid detection
WO2010046067A1 (en) 2008-10-20 2010-04-29 Roche Diagnostics Gmbh Improved allele-specific amplification

Also Published As

Publication number Publication date
CN105200097A (zh) 2015-12-30
CA2740913C (en) 2015-02-10
HK1219508A1 (zh) 2017-04-07
CN102186996A (zh) 2011-09-14
EP2337865A1 (en) 2011-06-29
EP2337865B1 (en) 2014-11-19
US9506111B2 (en) 2016-11-29
US8586299B2 (en) 2013-11-19
CA2740913A1 (en) 2010-04-29
WO2010046067A1 (en) 2010-04-29
US20140242592A1 (en) 2014-08-28
ES2527653T3 (es) 2015-01-28
US20100099110A1 (en) 2010-04-22
WO2010046067A9 (en) 2010-07-15
JP2012505641A (ja) 2012-03-08

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JP5624044B2 (ja) 改善されたアレル−特異的増幅
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US20160369330A1 (en) Compositions for improved allele-specific pcr
US9909169B2 (en) Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
HK1162054A (en) Improved allele-specific amplification

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