JP5624044B2 - 改善されたアレル−特異的増幅 - Google Patents

改善されたアレル−特異的増幅 Download PDF

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JP5624044B2
JP5624044B2 JP2011531404A JP2011531404A JP5624044B2 JP 5624044 B2 JP5624044 B2 JP 5624044B2 JP 2011531404 A JP2011531404 A JP 2011531404A JP 2011531404 A JP2011531404 A JP 2011531404A JP 5624044 B2 JP5624044 B2 JP 5624044B2
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oligonucleotide
target sequence
nucleotide
variant
complementary
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Japanese (ja)
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JP2012505641A5 (OSRAM
JP2012505641A (ja
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ゴードン ウィル，スティーブン
ゴードン ウィル，スティーブン
ニュートン，ニコラス
ツァン，アリソン
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2011531404A 2008-10-20 2009-10-17 改善されたアレル−特異的増幅 Active JP5624044B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10678308P 2008-10-20 2008-10-20
US61/106,783 2008-10-20
PCT/EP2009/007463 WO2010046067A1 (en) 2008-10-20 2009-10-17 Improved allele-specific amplification

Publications (3)

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JP2012505641A JP2012505641A (ja) 2012-03-08
JP2012505641A5 JP2012505641A5 (OSRAM) 2012-05-24
JP5624044B2 true JP5624044B2 (ja) 2014-11-12

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JP2011531404A Active JP5624044B2 (ja) 2008-10-20 2009-10-17 改善されたアレル−特異的増幅

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US (2) US8586299B2 (OSRAM)
EP (1) EP2337865B1 (OSRAM)
JP (1) JP5624044B2 (OSRAM)
CN (2) CN102186996A (OSRAM)
CA (1) CA2740913C (OSRAM)
ES (1) ES2527653T3 (OSRAM)
HK (1) HK1219508A1 (OSRAM)
WO (1) WO2010046067A1 (OSRAM)

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* Cited by examiner, † Cited by third party
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EP2337865B1 (en) 2008-10-20 2014-11-19 Roche Diagnostics GmbH Allele-specific amplification using a primer with a modified nucleotide
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
US9169514B2 (en) * 2010-12-03 2015-10-27 Brandeis University Detecting nucleic acid variations within populations of genomes
US20120164641A1 (en) 2010-12-22 2012-06-28 Roche Molecular Systems, Inc. Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene
EP2758546B1 (en) * 2011-09-23 2017-11-22 Roche Diagnostics GmbH Use of g-clamp for improved allele-specific pcr
EP2771486A1 (en) * 2011-10-24 2014-09-03 Trovagene, Inc. Methods of detecting braf mutations in cancer
US9738935B2 (en) 2011-11-10 2017-08-22 Roche Molecular Systems, Inc. Complex mutations in the epidermal growth factor receptor kinase domain
AU2012340118A1 (en) * 2011-11-17 2014-04-24 Rheonix, Inc. System and methods for selective molecular analysis
AU2013221642A1 (en) * 2012-02-15 2014-09-25 Janssen Diagnostics, Llc Highly sensitive method for detecting low frequency mutations
US20140341884A1 (en) 2012-12-04 2014-11-20 Roche Molecular Systems, Inc. Novel Complex Mutations in the Epidermal Growth Factor Receptor Kinase Domain
US9382581B2 (en) * 2012-12-13 2016-07-05 Roche Molecular Systems, Inc. Primers with modified phosphate and base in allele-specific PCR
CN108130373A (zh) * 2013-03-13 2018-06-08 豪夫迈·罗氏有限公司 在人pi3kca (pik3ca)基因中检测突变的方法和组合物
US10093978B2 (en) 2013-08-12 2018-10-09 Genentech, Inc. Compositions for detecting complement factor H (CFH) and complement factor I (CFI) polymorphisms
CN105593378B (zh) 2013-10-09 2019-09-20 豪夫迈·罗氏有限公司 用于在人ezh2基因中检测突变的方法和组合物
MX388168B (es) 2014-02-08 2025-03-19 Genentech Inc Un anticuerpo anti-amiloide beta (a?) monoclonal humanizado para usarse en el tratamiento de enfermedad de alzheimer.
CN103911445B (zh) * 2014-03-20 2015-08-12 上海科亦生物科技有限公司 一种as-pcr引物设计方法、基因多态性检测方法及试剂盒
PH12016502542B1 (en) * 2014-05-19 2024-01-17 Univ Rice William M Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
US9994914B2 (en) 2014-10-09 2018-06-12 Roche Molecular Systems, Inc. Mutation in the epidermal growth factor receptor kinase domain
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
CN104845967B (zh) * 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
CN105400900A (zh) * 2015-12-29 2016-03-16 杭州迪安生物技术有限公司 焦磷酸测序技术检测braf基因v600e微量突变试剂盒及其应用
JP2019519540A (ja) 2016-06-01 2019-07-11 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 肺癌患者におけるａｌｋ阻害薬療法に対する応答を予測する未分化リンパ腫キナーゼにおける新規な変異
US11384376B2 (en) * 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
CN110964818A (zh) * 2019-11-28 2020-04-07 重庆浦洛通基因医学研究院有限公司 一种人类braf基因v600e突变的检测试剂盒及检测方法
CN113215227A (zh) * 2021-02-04 2021-08-06 郑州华沃生物科技有限公司 一种人维生素d受体基因分型检测用的引物和探针

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DE3529478A1 (de) 1985-08-16 1987-02-19 Boehringer Mannheim Gmbh 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung
IE61148B1 (en) 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
US5521301A (en) 1988-12-12 1996-05-28 City Of Hope Genotyping of multiple allele systems
US5639611A (en) 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
US5137806A (en) 1989-12-11 1992-08-11 Board Of Regents, The University Of Texas System Methods and compositions for the detection of sequences in selected DNA molecules
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
ATE280177T1 (de) * 1997-03-20 2004-11-15 Hoffmann La Roche Modifizierte primer
EP0974672B1 (en) * 1998-07-21 2003-04-09 Keygene N.V. Improved primers for AFLP amplification
US6200757B1 (en) * 1999-01-19 2001-03-13 Dade Behring Inc. Method for controlling the extension of an oligonucleotide
JP3681729B2 (ja) 2001-02-15 2005-08-10 タカラバイオ株式会社 塩基置換の検出方法
JP2005524383A (ja) * 2001-08-28 2005-08-18 ファルマシア・アンド・アップジョン・カンパニー・エルエルシー 統合失調症の診断に用いる一塩基多型
US7408051B2 (en) 2004-04-14 2008-08-05 Applera Corporation Modified oligonucleotides and applications thereof
US8349556B2 (en) * 2006-04-28 2013-01-08 Igor Kutyavin Use of base-modified deoxynucleoside triphosphates to improve nucleic acid detection
EP2337865B1 (en) 2008-10-20 2014-11-19 Roche Diagnostics GmbH Allele-specific amplification using a primer with a modified nucleotide

Also Published As

Publication number Publication date
ES2527653T3 (es) 2015-01-28
CA2740913A1 (en) 2010-04-29
US20100099110A1 (en) 2010-04-22
US20140242592A1 (en) 2014-08-28
US9506111B2 (en) 2016-11-29
US8586299B2 (en) 2013-11-19
JP2012505641A (ja) 2012-03-08
EP2337865A1 (en) 2011-06-29
CN102186996A (zh) 2011-09-14
HK1219508A1 (zh) 2017-04-07
WO2010046067A9 (en) 2010-07-15
EP2337865B1 (en) 2014-11-19
CN105200097A (zh) 2015-12-30
CA2740913C (en) 2015-02-10
WO2010046067A1 (en) 2010-04-29

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US20160369330A1 (en) Compositions for improved allele-specific pcr
US9909169B2 (en) Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
HK1162054A (en) Improved allele-specific amplification

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