JP5622397B2 - 一本鎖抗体の配列に基づく操作および最適化 - Google Patents

一本鎖抗体の配列に基づく操作および最適化 Download PDF

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Publication number
JP5622397B2
JP5622397B2 JP2009553071A JP2009553071A JP5622397B2 JP 5622397 B2 JP5622397 B2 JP 5622397B2 JP 2009553071 A JP2009553071 A JP 2009553071A JP 2009553071 A JP2009553071 A JP 2009553071A JP 5622397 B2 JP5622397 B2 JP 5622397B2
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Japan
Prior art keywords
amino acid
database
scfv
positions
antibody
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Japanese (ja)
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JP2010523077A (ja
Inventor
ウレヒ，ダヴィッド
ボラス，レオナルド
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Esbatech a Novartis Co LLC
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Esbatech An Alcon Biomedical Research Unit LLC
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/567—Framework region [FR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/94—Stability, e.g. half-life, pH, temperature or enzyme-resistance
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2009553071A 2007-03-12 2008-03-12 一本鎖抗体の配列に基づく操作および最適化 Active JP5622397B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US90636507P 2007-03-12 2007-03-12
US60/906,365 2007-03-12
PCT/EP2008/001958 WO2008110348A1 (en) 2007-03-12 2008-03-12 Sequence based engineering and optimization of single chain antibodies

Publications (2)

Publication Number Publication Date
JP2010523077A JP2010523077A (ja) 2010-07-15
JP5622397B2 true JP5622397B2 (ja) 2014-11-12

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JP2009553071A Active JP5622397B2 (ja) 2007-03-12 2008-03-12 一本鎖抗体の配列に基づく操作および最適化

Country Status (11)

Country Link
US (2) US8280711B2 (enExample)
EP (2) EP2118138A1 (enExample)
JP (1) JP5622397B2 (enExample)
CN (2) CN103275216B (enExample)
AU (1) AU2008226067B2 (enExample)
CA (1) CA2680564C (enExample)
IL (1) IL200710A (enExample)
IN (1) IN2009DN05758A (enExample)
NZ (1) NZ579594A (enExample)
RU (2) RU2009137582A (enExample)
WO (1) WO2008110348A1 (enExample)

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JP2023507323A (ja) 2019-12-20 2023-02-22 アムジエン・インコーポレーテツド 固形腫瘍の治療のためのメソセリン標的化ｃｄ４０アゴニスト多重特異性抗体コンストラクト
JP2023538897A (ja) 2020-08-20 2023-09-12 アムジエン・インコーポレーテツド Ｆａｂ領域中に非標準的なジスルフィドを有する抗原結合タンパク質
US20230374162A1 (en) 2020-10-07 2023-11-23 Amgen Inc. Rational selection of building blocks for the assembly of multispecific antibodies
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MX2023009484A (es) 2021-02-12 2023-11-09 Boehringer Ingelheim Int Proteinas de union al antigeno complemento c3.
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Also Published As

Publication number Publication date
IL200710A (en) 2014-07-31
US8280711B2 (en) 2012-10-02
JP2010523077A (ja) 2010-07-15
CN103275216A (zh) 2013-09-04
IN2009DN05758A (enExample) 2015-07-24
CA2680564A1 (en) 2008-09-18
HK1138602A1 (en) 2010-08-27
US20100137150A1 (en) 2010-06-03
US9938336B2 (en) 2018-04-10
EP3202786A3 (en) 2017-10-11
CN101668771A (zh) 2010-03-10
US20130053259A1 (en) 2013-02-28
NZ579594A (en) 2012-03-30
RU2009137582A (ru) 2011-04-20
CN103275216B (zh) 2015-05-06
CN101668771B (zh) 2013-08-21
EP2118138A1 (en) 2009-11-18
AU2008226067A1 (en) 2008-09-18
IL200710A0 (en) 2010-05-17
EP3202786A2 (en) 2017-08-09
RU2014127308A (ru) 2016-02-10
WO2008110348A1 (en) 2008-09-18
CA2680564C (en) 2018-05-01
AU2008226067B2 (en) 2012-11-08

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