JP5618413B2 - 大型核酸のアッセンブリー - Google Patents

大型核酸のアッセンブリー Download PDF

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Publication number
JP5618413B2
JP5618413B2 JP2010528976A JP2010528976A JP5618413B2 JP 5618413 B2 JP5618413 B2 JP 5618413B2 JP 2010528976 A JP2010528976 A JP 2010528976A JP 2010528976 A JP2010528976 A JP 2010528976A JP 5618413 B2 JP5618413 B2 JP 5618413B2
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nucleic acid
fragments
dna
assembled
genome
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JP2010528976A
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Japanese (ja)
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JP2010539994A5 (enExample
JP2010539994A (ja
Inventor
ジー． ギブソン、ダニエル
ジー． ギブソン、ダニエル
エー． ベンダーズ、グウィネズ
エー． ベンダーズ、グウィネズ
ヤング、レイ
アイ． グラス、ジョン
アイ． グラス、ジョン
ベンター、ジェイ．クレイグ
エー． ハッチンソン、Ｉｉｉ、クライド
エー． ハッチンソン、Ｉｉｉ、クライド
オー． スミス、ハミルトン
オー． スミス、ハミルトン
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シンセティック ゲノミクス、インク．
シンセティック ゲノミクス、インク．
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2010528976A 2007-10-08 2008-10-07 大型核酸のアッセンブリー Expired - Fee Related JP5618413B2 (ja)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US97838807P 2007-10-08 2007-10-08
US60/978,388 2007-10-08
US98354907P 2007-10-29 2007-10-29
US60/983,549 2007-10-29
US6221408P 2008-01-23 2008-01-23
US61/062,214 2008-01-23
US2339208P 2008-01-24 2008-01-24
US61/023,392 2008-01-24
US9627008P 2008-09-11 2008-09-11
US61/096,270 2008-09-11
PCT/US2008/079109 WO2009048885A2 (en) 2007-10-08 2008-10-07 Assembly of large nucleic acids

Publications (3)

Publication Number Publication Date
JP2010539994A JP2010539994A (ja) 2010-12-24
JP2010539994A5 JP2010539994A5 (enExample) 2011-08-25
JP5618413B2 true JP5618413B2 (ja) 2014-11-05

Family

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Family Applications (1)

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JP2010528976A Expired - Fee Related JP5618413B2 (ja) 2007-10-08 2008-10-07 大型核酸のアッセンブリー

Country Status (11)

Country Link
US (1) US9593329B2 (enExample)
EP (1) EP2207899B1 (enExample)
JP (1) JP5618413B2 (enExample)
CN (1) CN101932725A (enExample)
AU (1) AU2008311000B2 (enExample)
CA (1) CA2701923C (enExample)
DK (1) DK2207899T3 (enExample)
IL (1) IL204892B (enExample)
MX (1) MX2010003644A (enExample)
MY (1) MY161418A (enExample)
WO (1) WO2009048885A2 (enExample)

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US9267132B2 (en) 2007-10-08 2016-02-23 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
WO2010045326A1 (en) 2008-10-14 2010-04-22 Barnes Wayne M In vitro recombination methods using 5' exonuclease
AU2009316660B2 (en) * 2008-11-19 2015-01-29 Amrys, Inc. Compositions and methods for the assembly of polynucleotides
AU2010221172B2 (en) 2009-03-06 2015-06-04 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
US9821052B2 (en) 2009-07-31 2017-11-21 Seqirus UK Limited Reverse genetics systems
US20110269119A1 (en) 2009-10-30 2011-11-03 Synthetic Genomics, Inc. Encoding text into nucleic acid sequences
JP2013520989A (ja) 2010-03-05 2013-06-10 シンセティック ジェノミクス インコーポレーテッド ゲノムのクローニングおよび操作のための方法
US9689012B2 (en) 2010-10-12 2017-06-27 Cornell University Method of dual-adapter recombination for efficient concatenation of multiple DNA fragments in shuffled or specified arrangements
EP2768607B1 (en) 2011-09-26 2021-08-18 Thermo Fisher Scientific GENEART GmbH Multiwell plate for high efficiency, small volume nucleic acid synthesis
US20140303036A1 (en) * 2011-11-23 2014-10-09 Dsm Ip Assets B.V. Nucleic Acid Assembly System
WO2013191950A2 (en) 2012-06-22 2013-12-27 Monsanto Technology Llc Unique modular vector design
WO2014036404A2 (en) * 2012-08-31 2014-03-06 Synthetic Genomics, Inc. Crowding agent-induced nucleic acid transfer into a recipient host cell
CA2893817C (en) * 2012-12-13 2021-03-09 Synthetic Genomics, Inc. Peg-mediated assembly of nucleic acid molecules
SG11201505550RA (en) * 2013-01-23 2015-08-28 Novartis Ag Influenza virus reassortment
US20140274806A1 (en) 2013-03-15 2014-09-18 Synthetic Genomics Vaccines Influenza virus reassortment
US10563240B2 (en) 2013-03-14 2020-02-18 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US20170198268A1 (en) 2014-07-09 2017-07-13 Gen9, Inc. Compositions and Methods for Site-Directed DNA Nicking and Cleaving
CA3147259A1 (en) 2014-12-09 2016-06-16 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
CN106282224B (zh) * 2015-06-26 2020-05-01 深圳华大生命科学研究院 在靶细胞中构建人工染色体的方法以及真核细胞
EP3359669B1 (en) 2015-10-06 2020-05-13 Pierce Biotechnology, Inc. Devices and methods for producing proteins
CA3018199A1 (en) 2016-03-23 2017-09-28 Synthetic Genomics, Inc. Generation of synthetic genomes
CN110100011A (zh) * 2016-11-21 2019-08-06 诺维信公司 Dna分子的酵母细胞提取物辅助构建
US10726110B2 (en) * 2017-03-01 2020-07-28 Seven Bridges Genomics, Inc. Watermarking for data security in bioinformatic sequence analysis
US20210147830A1 (en) 2018-06-29 2021-05-20 Thermo Fisher Scientific Geneart Gmbh High throughput assembly of nucleic acid molecules
JP7430701B2 (ja) 2018-07-23 2024-02-13 ディーエヌエー スクリプト 核酸鎖の大規模並列酵素合成
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
US20220235378A1 (en) * 2019-05-06 2022-07-28 Dsm Ip Assets B.V. Multipartite crispr donor
EP4114972A1 (en) 2020-03-06 2023-01-11 Life Technologies Corporation High sequence fidelity nucleic acid synthesis and assembly
CN113380322B (zh) * 2021-06-25 2023-10-24 倍生生物科技(深圳)有限公司 人工核酸序列水印编码系统、水印字符串及编码和解码方法
WO2024132094A1 (en) 2022-12-19 2024-06-27 Thermo Fisher Scientific Geneart Gmbh Retrieval of sequence-verified nucleic acid molecules

Family Cites Families (13)

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US6348353B1 (en) * 1995-06-07 2002-02-19 Case Western Reserve University Artificial mammalian chromosome
US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
AU6487196A (en) 1996-07-09 1998-02-02 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Transformation-associated recombination cloning
ES2243007T3 (es) 1997-09-16 2005-11-16 Egea Biosciences Llc Sintesis quimica completa y sintesis de genes de genomas.
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EP2239327B1 (en) * 2005-08-11 2015-02-25 Synthetic Genomics, Inc. Method for in vitro recombination
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JP5666908B2 (ja) 2007-10-05 2015-02-12 サファイア エナジー，インコーポレイティド ゲノムｄｎａの大きな断片の捕捉および修飾、ならびに合成葉緑体を有する生物を構築するための系

Also Published As

Publication number Publication date
MY161418A (en) 2017-04-14
EP2207899A4 (en) 2010-12-01
US20090275086A1 (en) 2009-11-05
WO2009048885A3 (en) 2009-12-30
JP2010539994A (ja) 2010-12-24
AU2008311000A1 (en) 2009-04-16
EP2207899A2 (en) 2010-07-21
CA2701923A1 (en) 2009-04-16
CN101932725A (zh) 2010-12-29
MX2010003644A (es) 2010-06-23
US9593329B2 (en) 2017-03-14
IL204892A0 (en) 2010-11-30
AU2008311000B2 (en) 2013-12-19
CA2701923C (en) 2018-07-10
DK2207899T3 (en) 2018-09-03
IL204892B (en) 2018-02-28
EP2207899B1 (en) 2018-04-18
WO2009048885A2 (en) 2009-04-16
AU2008311000A2 (en) 2010-06-03

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