AU2008311000B2 - Assembly of large nucleic acids - Google Patents

Assembly of large nucleic acids Download PDF

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Publication number
AU2008311000B2
AU2008311000B2 AU2008311000A AU2008311000A AU2008311000B2 AU 2008311000 B2 AU2008311000 B2 AU 2008311000B2 AU 2008311000 A AU2008311000 A AU 2008311000A AU 2008311000 A AU2008311000 A AU 2008311000A AU 2008311000 B2 AU2008311000 B2 AU 2008311000B2
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AU
Australia
Prior art keywords
nucleic acid
fragments
acid molecule
assembled
dna
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Ceased
Application number
AU2008311000A
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English (en)
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AU2008311000A1 (en
AU2008311000A2 (en
Inventor
Gwynedd A. Benders
Daniel G. Gibson
John I. Glass
Clyde A. Hutchison
Hamilton O. Smith
J. Craig Venter
Lei Young
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Viridos Inc
Original Assignee
Synthetic Genomics Inc
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Publication of AU2008311000A1 publication Critical patent/AU2008311000A1/en
Publication of AU2008311000A2 publication Critical patent/AU2008311000A2/en
Application granted granted Critical
Publication of AU2008311000B2 publication Critical patent/AU2008311000B2/en
Ceased legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Ecology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2008311000A 2007-10-08 2008-10-07 Assembly of large nucleic acids Ceased AU2008311000B2 (en)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US97838807P 2007-10-08 2007-10-08
US60/978,388 2007-10-08
US98354907P 2007-10-29 2007-10-29
US60/983,549 2007-10-29
US6221408P 2008-01-23 2008-01-23
US61/062,214 2008-01-23
US2339208P 2008-01-24 2008-01-24
US61/023,392 2008-01-24
US9627008P 2008-09-11 2008-09-11
US61/096,270 2008-09-11
PCT/US2008/079109 WO2009048885A2 (en) 2007-10-08 2008-10-07 Assembly of large nucleic acids

Publications (3)

Publication Number Publication Date
AU2008311000A1 AU2008311000A1 (en) 2009-04-16
AU2008311000A2 AU2008311000A2 (en) 2010-06-03
AU2008311000B2 true AU2008311000B2 (en) 2013-12-19

Family

ID=40549815

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2008311000A Ceased AU2008311000B2 (en) 2007-10-08 2008-10-07 Assembly of large nucleic acids

Country Status (11)

Country Link
US (1) US9593329B2 (enExample)
EP (1) EP2207899B1 (enExample)
JP (1) JP5618413B2 (enExample)
CN (1) CN101932725A (enExample)
AU (1) AU2008311000B2 (enExample)
CA (1) CA2701923C (enExample)
DK (1) DK2207899T3 (enExample)
IL (1) IL204892B (enExample)
MX (1) MX2010003644A (enExample)
MY (1) MY161418A (enExample)
WO (1) WO2009048885A2 (enExample)

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US9267132B2 (en) 2007-10-08 2016-02-23 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
WO2010045326A1 (en) 2008-10-14 2010-04-22 Barnes Wayne M In vitro recombination methods using 5' exonuclease
EP2358875B1 (en) 2008-11-19 2015-08-12 Amyris, Inc. Compositions and methods for the assembly of polynucleotides
EP3249045A1 (en) 2009-03-06 2017-11-29 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
WO2011012999A1 (en) * 2009-07-31 2011-02-03 Novartis Ag Reverse genetics systems
US20110269119A1 (en) 2009-10-30 2011-11-03 Synthetic Genomics, Inc. Encoding text into nucleic acid sequences
CN102939380A (zh) * 2010-03-05 2013-02-20 合成基因组股份有限公司 用于克隆和操作基因组的方法
US9689012B2 (en) 2010-10-12 2017-06-27 Cornell University Method of dual-adapter recombination for efficient concatenation of multiple DNA fragments in shuffled or specified arrangements
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
LT2768607T (lt) 2011-09-26 2021-12-27 Thermo Fisher Scientific Geneart Gmbh Daugiašulininė plokštelė didelio efektyvumo, mažo tūrio nukleorūgščių sintezei atlikti
US12595500B2 (en) 2011-09-26 2026-04-07 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
EP2783000A1 (en) * 2011-11-23 2014-10-01 DSM IP Assets B.V. Nucleic acid assembly system
US10829772B2 (en) 2012-06-22 2020-11-10 Monsanto Technology Llc Unique modular vector design
WO2014036404A2 (en) * 2012-08-31 2014-03-06 Synthetic Genomics, Inc. Crowding agent-induced nucleic acid transfer into a recipient host cell
CN104822840A (zh) * 2012-12-13 2015-08-05 合成基因组股份有限公司 Peg-介导的核酸分子的组装
US20140274806A1 (en) 2013-03-15 2014-09-18 Synthetic Genomics Vaccines Influenza virus reassortment
CA2898961A1 (en) * 2013-01-23 2014-07-31 Novartis Ag Influenza virus reassortment
WO2016007604A1 (en) 2014-07-09 2016-01-14 Gen9, Inc. Compositions and methods for site-directed dna nicking and cleaving
WO2016094512A1 (en) 2014-12-09 2016-06-16 yLIFE TECHNOLOGIES CORPORATION High efficiency, small volume nucleic acid synthesis
CN106282224B (zh) * 2015-06-26 2020-05-01 深圳华大生命科学研究院 在靶细胞中构建人工染色体的方法以及真核细胞
EP3763818A1 (en) 2015-10-06 2021-01-13 Pierce Biotechnology, Inc. Devices and methods for producing proteins
CA3018199A1 (en) 2016-03-23 2017-09-28 Synthetic Genomics, Inc. Generation of synthetic genomes
EP3541954A1 (en) * 2016-11-21 2019-09-25 Novozymes A/S Yeast cell extract assisted construction of dna molecules
US10726110B2 (en) * 2017-03-01 2020-07-28 Seven Bridges Genomics, Inc. Watermarking for data security in bioinformatic sequence analysis
US20210147830A1 (en) 2018-06-29 2021-05-20 Thermo Fisher Scientific Geneart Gmbh High throughput assembly of nucleic acid molecules
JP7430701B2 (ja) 2018-07-23 2024-02-13 ディーエヌエー スクリプト 核酸鎖の大規模並列酵素合成
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
EP3966324A1 (en) * 2019-05-06 2022-03-16 DSM IP Assets B.V. Multipartite crispr donor
EP4114972A1 (en) 2020-03-06 2023-01-11 Life Technologies Corporation High sequence fidelity nucleic acid synthesis and assembly
CN113380322B (zh) * 2021-06-25 2023-10-24 倍生生物科技(深圳)有限公司 人工核酸序列水印编码系统、水印字符串及编码和解码方法
WO2024132094A1 (en) 2022-12-19 2024-06-27 Thermo Fisher Scientific Geneart Gmbh Retrieval of sequence-verified nucleic acid molecules

Citations (1)

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Publication number Priority date Publication date Assignee Title
US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping

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US6348353B1 (en) * 1995-06-07 2002-02-19 Case Western Reserve University Artificial mammalian chromosome
WO1998001573A1 (en) 1996-07-09 1998-01-15 The Government Of The United States Of America Represented By The Secretary, Department Of Health And Human Services Transformation-associated recombination cloning
ES2243007T3 (es) * 1997-09-16 2005-11-16 Egea Biosciences Llc Sintesis quimica completa y sintesis de genes de genomas.
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EP1629097A1 (en) * 2003-05-22 2006-03-01 University of California Method for producing a synthetic gene or other dna sequence
WO2007032837A2 (en) * 2005-08-11 2007-03-22 The J. Craig Venter Institute Method for in vitro recombination
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US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping

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Also Published As

Publication number Publication date
WO2009048885A3 (en) 2009-12-30
IL204892B (en) 2018-02-28
MY161418A (en) 2017-04-14
EP2207899A4 (en) 2010-12-01
US9593329B2 (en) 2017-03-14
JP5618413B2 (ja) 2014-11-05
CN101932725A (zh) 2010-12-29
MX2010003644A (es) 2010-06-23
US20090275086A1 (en) 2009-11-05
AU2008311000A1 (en) 2009-04-16
CA2701923A1 (en) 2009-04-16
JP2010539994A (ja) 2010-12-24
CA2701923C (en) 2018-07-10
EP2207899A2 (en) 2010-07-21
IL204892A0 (en) 2010-11-30
EP2207899B1 (en) 2018-04-18
WO2009048885A2 (en) 2009-04-16
DK2207899T3 (en) 2018-09-03
AU2008311000A2 (en) 2010-06-03

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