CN101932725A - 大核酸的装配 - Google Patents

大核酸的装配 Download PDF

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Publication number
CN101932725A
CN101932725A CN200880119758XA CN200880119758A CN101932725A CN 101932725 A CN101932725 A CN 101932725A CN 200880119758X A CN200880119758X A CN 200880119758XA CN 200880119758 A CN200880119758 A CN 200880119758A CN 101932725 A CN101932725 A CN 101932725A
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China
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dna
fragments
culture
yeast
assembled
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CN200880119758XA
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Chinese (zh)
Inventor
丹尼尔·G·吉布森
格威内德·A·本德斯
杨磊
约翰·I·格拉斯
J·克雷格·文特尔
克莱德·A·哈奇森
汉密尔顿·O·史密斯
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Viridos Inc
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Synthetic Genomics Inc
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN200880119758XA 2007-10-08 2008-10-07 大核酸的装配 Pending CN101932725A (zh)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US97838807P 2007-10-08 2007-10-08
US60/978,388 2007-10-08
US98354907P 2007-10-29 2007-10-29
US60/983,549 2007-10-29
US6221408P 2008-01-23 2008-01-23
US61/062,214 2008-01-23
US2339208P 2008-01-24 2008-01-24
US61/023,392 2008-01-24
US9627008P 2008-09-11 2008-09-11
US61/096,270 2008-09-11
PCT/US2008/079109 WO2009048885A2 (en) 2007-10-08 2008-10-07 Assembly of large nucleic acids

Publications (1)

Publication Number Publication Date
CN101932725A true CN101932725A (zh) 2010-12-29

Family

ID=40549815

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200880119758XA Pending CN101932725A (zh) 2007-10-08 2008-10-07 大核酸的装配

Country Status (11)

Country Link
US (1) US9593329B2 (enExample)
EP (1) EP2207899B1 (enExample)
JP (1) JP5618413B2 (enExample)
CN (1) CN101932725A (enExample)
AU (1) AU2008311000B2 (enExample)
CA (1) CA2701923C (enExample)
DK (1) DK2207899T3 (enExample)
IL (1) IL204892B (enExample)
MX (1) MX2010003644A (enExample)
MY (1) MY161418A (enExample)
WO (1) WO2009048885A2 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
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CN106282224A (zh) * 2015-06-26 2017-01-04 深圳华大基因研究院 在靶细胞中构建人工染色体的方法以及真核细胞
CN109913519A (zh) * 2012-12-13 2019-06-21 合成基因组股份有限公司 Peg-介导的核酸分子的组装
CN113380322A (zh) * 2021-06-25 2021-09-10 倍生生物科技(深圳)有限公司 人工核酸序列水印编码系统、水印字符串及编码和解码方法

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US9267132B2 (en) 2007-10-08 2016-02-23 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
WO2010045326A1 (en) 2008-10-14 2010-04-22 Barnes Wayne M In vitro recombination methods using 5' exonuclease
WO2010059763A1 (en) 2008-11-19 2010-05-27 Amyris Biotechnologies, Inc. Compositions and methods for the assembly of polynucleotides
EP2403944B1 (en) 2009-03-06 2017-06-28 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
WO2011012999A1 (en) * 2009-07-31 2011-02-03 Novartis Ag Reverse genetics systems
US20110269119A1 (en) 2009-10-30 2011-11-03 Synthetic Genomics, Inc. Encoding text into nucleic acid sequences
JP2013520989A (ja) * 2010-03-05 2013-06-10 シンセティック ジェノミクス インコーポレーテッド ゲノムのクローニングおよび操作のための方法
US9689012B2 (en) 2010-10-12 2017-06-27 Cornell University Method of dual-adapter recombination for efficient concatenation of multiple DNA fragments in shuffled or specified arrangements
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
EP3964285A1 (en) 2011-09-26 2022-03-09 Thermo Fisher Scientific Geneart GmbH High efficiency, small volume nucleic acid synthesis
US20140303036A1 (en) * 2011-11-23 2014-10-09 Dsm Ip Assets B.V. Nucleic Acid Assembly System
WO2013191950A2 (en) 2012-06-22 2013-12-27 Monsanto Technology Llc Unique modular vector design
WO2014036404A2 (en) * 2012-08-31 2014-03-06 Synthetic Genomics, Inc. Crowding agent-induced nucleic acid transfer into a recipient host cell
US20140274806A1 (en) 2013-03-15 2014-09-18 Synthetic Genomics Vaccines Influenza virus reassortment
HK1217638A1 (zh) * 2013-01-23 2017-01-20 Novartis Ag 流感病毒重配
EP3167071B1 (en) 2014-07-09 2020-10-07 Gen9, Inc. Compositions and methods for site-directed dna nicking and cleaving
US10407676B2 (en) 2014-12-09 2019-09-10 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
WO2017062343A1 (en) 2015-10-06 2017-04-13 Pierce Biotechnology, Inc. Devices and methods for producing nucleic acids and proteins
CA3018199A1 (en) 2016-03-23 2017-09-28 Synthetic Genomics, Inc. Generation of synthetic genomes
CN110100011A (zh) * 2016-11-21 2019-08-06 诺维信公司 Dna分子的酵母细胞提取物辅助构建
US10726110B2 (en) * 2017-03-01 2020-07-28 Seven Bridges Genomics, Inc. Watermarking for data security in bioinformatic sequence analysis
US20210147830A1 (en) 2018-06-29 2021-05-20 Thermo Fisher Scientific Geneart Gmbh High throughput assembly of nucleic acid molecules
WO2020020608A1 (en) 2018-07-23 2020-01-30 Dna Script Massively parallel enzymatic synthesis of nucleic acid strands
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
WO2020224987A1 (en) * 2019-05-06 2020-11-12 Dsm Ip Assets B.V. Multipartite crispr donor
JP2023516827A (ja) 2020-03-06 2023-04-20 ライフ テクノロジーズ コーポレイション 高配列忠実度の核酸合成およびアセンブリ
WO2024132094A1 (en) 2022-12-19 2024-06-27 Thermo Fisher Scientific Geneart Gmbh Retrieval of sequence-verified nucleic acid molecules

Citations (4)

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CN1191574A (zh) * 1995-06-07 1998-08-26 凯斯韦斯顿瑞瑟弗大学 合成的哺乳动物染色体和其构建方法
US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
US20030165946A1 (en) * 1997-09-16 2003-09-04 Egea Biosciences, Inc. Method for the complete chemical synthesis and assembly of genes and genomes
US20070037196A1 (en) * 2005-08-11 2007-02-15 The J. Craig Venter Institute, Inc. Method for in vitro recombination

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WO1998001573A1 (en) 1996-07-09 1998-01-15 The Government Of The United States Of America Represented By The Secretary, Department Of Health And Human Services Transformation-associated recombination cloning
AU5217599A (en) 1998-07-27 2000-02-21 Genotypes, Inc. Automatic eukaryotic artificial chromosome vector
AU5597200A (en) * 1999-06-07 2000-12-28 Cell Genesys, Inc. Hybrid yeast-bacteria cloning system and uses thereof
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JP2009511051A (ja) 2005-10-12 2009-03-19 ザ ジェイ． クレイグ ベンター インスティテュート 最小限の細菌ゲノム
KR101389254B1 (ko) 2007-10-05 2014-04-24 사파이어 에너지, 인크. 거대 게놈 ｄｎａ의 포획 및 변형 및 합성 엽록체를 갖는 생물의 구성을 위한 시스템

Patent Citations (4)

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CN1191574A (zh) * 1995-06-07 1998-08-26 凯斯韦斯顿瑞瑟弗大学 合成的哺乳动物染色体和其构建方法
US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
US20030165946A1 (en) * 1997-09-16 2003-09-04 Egea Biosciences, Inc. Method for the complete chemical synthesis and assembly of genes and genomes
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NATALAY KOUPRINE ETC: "《Segments missing from the draft human genome sequence can be isolated by transformation-associated recombination cloning in yease》", 《EMBO REPORTS》 *
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109913519A (zh) * 2012-12-13 2019-06-21 合成基因组股份有限公司 Peg-介导的核酸分子的组装
CN109913519B (zh) * 2012-12-13 2022-12-02 合成基因组股份有限公司 Peg-介导的核酸分子的组装
CN106282224A (zh) * 2015-06-26 2017-01-04 深圳华大基因研究院 在靶细胞中构建人工染色体的方法以及真核细胞
CN106282224B (zh) * 2015-06-26 2020-05-01 深圳华大生命科学研究院 在靶细胞中构建人工染色体的方法以及真核细胞
CN113380322A (zh) * 2021-06-25 2021-09-10 倍生生物科技(深圳)有限公司 人工核酸序列水印编码系统、水印字符串及编码和解码方法
CN113380322B (zh) * 2021-06-25 2023-10-24 倍生生物科技(深圳)有限公司 人工核酸序列水印编码系统、水印字符串及编码和解码方法

Also Published As

Publication number Publication date
JP2010539994A (ja) 2010-12-24
JP5618413B2 (ja) 2014-11-05
DK2207899T3 (en) 2018-09-03
US9593329B2 (en) 2017-03-14
CA2701923C (en) 2018-07-10
WO2009048885A2 (en) 2009-04-16
US20090275086A1 (en) 2009-11-05
AU2008311000A2 (en) 2010-06-03
EP2207899A4 (en) 2010-12-01
AU2008311000B2 (en) 2013-12-19
EP2207899A2 (en) 2010-07-21
MY161418A (en) 2017-04-14
IL204892A0 (en) 2010-11-30
MX2010003644A (es) 2010-06-23
IL204892B (en) 2018-02-28
CA2701923A1 (en) 2009-04-16
AU2008311000A1 (en) 2009-04-16
EP2207899B1 (en) 2018-04-18
WO2009048885A3 (en) 2009-12-30

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Application publication date: 20101229