DK2207899T3 - COLLECTION OF LARGE NUCLEIC ACIDS - Google Patents

COLLECTION OF LARGE NUCLEIC ACIDS Download PDF

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Publication number
DK2207899T3
DK2207899T3 DK08838436.7T DK08838436T DK2207899T3 DK 2207899 T3 DK2207899 T3 DK 2207899T3 DK 08838436 T DK08838436 T DK 08838436T DK 2207899 T3 DK2207899 T3 DK 2207899T3
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Prior art keywords
dna
fragments
cassettes
yeast
genome
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DK08838436.7T
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Danish (da)
English (en)
Inventor
J Craig Venter
Daniel G Gibson
Lei Young
John I Glass
Clyde A Hutchison
Hamilton O Smith
Gwynedd A Benders
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Synthetic Genomics Inc
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Publication of DK2207899T3 publication Critical patent/DK2207899T3/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1027Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1058Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Ecology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK08838436.7T 2007-10-08 2008-10-07 COLLECTION OF LARGE NUCLEIC ACIDS DK2207899T3 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US97838807P 2007-10-08 2007-10-08
US98354907P 2007-10-29 2007-10-29
US6221408P 2008-01-23 2008-01-23
US2339208P 2008-01-24 2008-01-24
US9627008P 2008-09-11 2008-09-11
PCT/US2008/079109 WO2009048885A2 (en) 2007-10-08 2008-10-07 Assembly of large nucleic acids

Publications (1)

Publication Number Publication Date
DK2207899T3 true DK2207899T3 (en) 2018-09-03

Family

ID=40549815

Family Applications (1)

Application Number Title Priority Date Filing Date
DK08838436.7T DK2207899T3 (en) 2007-10-08 2008-10-07 COLLECTION OF LARGE NUCLEIC ACIDS

Country Status (11)

Country Link
US (1) US9593329B2 (enExample)
EP (1) EP2207899B1 (enExample)
JP (1) JP5618413B2 (enExample)
CN (1) CN101932725A (enExample)
AU (1) AU2008311000B2 (enExample)
CA (1) CA2701923C (enExample)
DK (1) DK2207899T3 (enExample)
IL (1) IL204892B (enExample)
MX (1) MX2010003644A (enExample)
MY (1) MY161418A (enExample)
WO (1) WO2009048885A2 (enExample)

Families Citing this family (30)

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US9267132B2 (en) 2007-10-08 2016-02-23 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
US9234226B2 (en) 2008-10-14 2016-01-12 Wayne M. Barnes In vitro recombination methods using 5′ exonuclease
JP2012509084A (ja) 2008-11-19 2012-04-19 アムイリス, インコーポレイテッド ポリヌクレオチドアセンブリに関する組成物及び方法
EP2403944B1 (en) 2009-03-06 2017-06-28 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
CN102666860B (zh) 2009-07-31 2015-06-17 诺华股份有限公司 反向遗传系统
US20110269119A1 (en) 2009-10-30 2011-11-03 Synthetic Genomics, Inc. Encoding text into nucleic acid sequences
EP2542676A1 (en) * 2010-03-05 2013-01-09 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
WO2012051327A2 (en) 2010-10-12 2012-04-19 Cornell University Method of dual-adapter recombination for efficient concatenation of multiple dna fragments in shuffled or specified arrangements
US20130109596A1 (en) 2011-09-26 2013-05-02 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
WO2013076280A1 (en) * 2011-11-23 2013-05-30 Dsm Ip Assets B.V. Nucleic acid assembly system
US10829772B2 (en) 2012-06-22 2020-11-10 Monsanto Technology Llc Unique modular vector design
WO2014036404A2 (en) * 2012-08-31 2014-03-06 Synthetic Genomics, Inc. Crowding agent-induced nucleic acid transfer into a recipient host cell
SG11201504584VA (en) * 2012-12-13 2015-07-30 Synthetic Genomics Inc Peg-mediated assembly of nucleic acid molecules
US20140274806A1 (en) 2013-03-15 2014-09-18 Synthetic Genomics Vaccines Influenza virus reassortment
MX2015009330A (es) * 2013-01-23 2016-04-15 Novartis Ag Reordenamiento del virus de influenza.
US10563240B2 (en) 2013-03-14 2020-02-18 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
EP3167071B1 (en) 2014-07-09 2020-10-07 Gen9, Inc. Compositions and methods for site-directed dna nicking and cleaving
CN115927293A (zh) 2014-12-09 2023-04-07 生命技术公司 高效小体积核酸合成
CN106282224B (zh) * 2015-06-26 2020-05-01 深圳华大生命科学研究院 在靶细胞中构建人工染色体的方法以及真核细胞
EP3359669B1 (en) 2015-10-06 2020-05-13 Pierce Biotechnology, Inc. Devices and methods for producing proteins
EP3433354B1 (en) 2016-03-23 2023-12-27 Synthetic Genomics, Inc. Generation of synthetic genomes
WO2018094181A1 (en) * 2016-11-21 2018-05-24 Novozymes A/S Yeast cell extract assisted construction of dna molecules
US10726110B2 (en) * 2017-03-01 2020-07-28 Seven Bridges Genomics, Inc. Watermarking for data security in bioinformatic sequence analysis
EP3814494B1 (en) 2018-06-29 2023-11-01 Thermo Fisher Scientific GENEART GmbH High throughput assembly of nucleic acid molecules
EP3826762A1 (en) 2018-07-23 2021-06-02 DNA Script Massively parallel enzymatic synthesis of nucleic acid strands
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
WO2020224987A1 (en) * 2019-05-06 2020-11-12 Dsm Ip Assets B.V. Multipartite crispr donor
US20240025939A1 (en) 2020-03-06 2024-01-25 Life Technologies Corporation High sequence fidelity nucleic acid synthesis and assembly
CN113380322B (zh) * 2021-06-25 2023-10-24 倍生生物科技(深圳)有限公司 人工核酸序列水印编码系统、水印字符串及编码和解码方法
EP4638776A1 (en) 2022-12-19 2025-10-29 Thermo Fisher Scientific GENEART GmbH Retrieval of sequence-verified nucleic acid molecules

Family Cites Families (13)

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US6348353B1 (en) * 1995-06-07 2002-02-19 Case Western Reserve University Artificial mammalian chromosome
US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
AU6487196A (en) 1996-07-09 1998-02-02 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Transformation-associated recombination cloning
DK1015576T3 (da) 1997-09-16 2005-08-29 Egea Biosciences Llc Fremgangsmåde til fuldstændig kemisk syntese og aggregering af gener og genomer
AU5217599A (en) 1998-07-27 2000-02-21 Genotypes, Inc. Automatic eukaryotic artificial chromosome vector
US7083971B1 (en) 1999-06-07 2006-08-01 Cell Genesys, Inc. Hybrid yeast-bacteria cloning system and uses thereof
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AU2002332027A1 (en) * 2001-10-04 2003-04-14 The Government Of The United States Of America, As Tandem repeat markers
JP2007534303A (ja) * 2003-05-22 2007-11-29 ユニバーシティー オブ カリフォルニア 合成遺伝子または他のdna配列を作製するための方法
CA2618699C (en) 2005-08-11 2012-10-02 J. Craig Venter Institute, Inc. In vitro recombination method
CA2618665C (en) 2005-08-11 2012-11-13 J. Craig Venter Institute Method for in vitro recombination
AU2006303957A1 (en) 2005-10-12 2007-04-26 Synthetic Genomics, Inc. Minimal bacterial genome
GB2460351B (en) 2007-10-05 2011-03-30 Sapphire Energy Inc System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts

Also Published As

Publication number Publication date
IL204892B (en) 2018-02-28
IL204892A0 (en) 2010-11-30
US9593329B2 (en) 2017-03-14
CA2701923C (en) 2018-07-10
WO2009048885A3 (en) 2009-12-30
EP2207899B1 (en) 2018-04-18
MX2010003644A (es) 2010-06-23
EP2207899A2 (en) 2010-07-21
AU2008311000B2 (en) 2013-12-19
CN101932725A (zh) 2010-12-29
CA2701923A1 (en) 2009-04-16
MY161418A (en) 2017-04-14
JP5618413B2 (ja) 2014-11-05
US20090275086A1 (en) 2009-11-05
AU2008311000A2 (en) 2010-06-03
JP2010539994A (ja) 2010-12-24
EP2207899A4 (en) 2010-12-01
WO2009048885A2 (en) 2009-04-16
AU2008311000A1 (en) 2009-04-16

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