JP5574400B2 - 変異型リパーゼとその応用 - Google Patents

変異型リパーゼとその応用 Download PDF

Info

Publication number
JP5574400B2
JP5574400B2 JP2008331771A JP2008331771A JP5574400B2 JP 5574400 B2 JP5574400 B2 JP 5574400B2 JP 2008331771 A JP2008331771 A JP 2008331771A JP 2008331771 A JP2008331771 A JP 2008331771A JP 5574400 B2 JP5574400 B2 JP 5574400B2
Authority
JP
Japan
Prior art keywords
mutation
amino acid
cryptococcus
lipase
replaced
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2008331771A
Other languages
English (en)
Japanese (ja)
Other versions
JP2012044869A (ja
Inventor
國夫 中田
佳弘 臼田
信久 榛葉
亘 星野
榮一郎 鈴木
治幸 家藤
和夫 正木
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Institute of Brewing
Original Assignee
National Research Institute of Brewing
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Research Institute of Brewing filed Critical National Research Institute of Brewing
Priority to JP2008331771A priority Critical patent/JP5574400B2/ja
Priority to PCT/JP2009/071553 priority patent/WO2010074209A1/fr
Publication of JP2012044869A publication Critical patent/JP2012044869A/ja
Application granted granted Critical
Publication of JP5574400B2 publication Critical patent/JP5574400B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/32—Processes using, or culture media containing, lower alkanols, i.e. C1 to C6
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/227—Tryptophan

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2008331771A 2008-12-26 2008-12-26 変異型リパーゼとその応用 Expired - Fee Related JP5574400B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2008331771A JP5574400B2 (ja) 2008-12-26 2008-12-26 変異型リパーゼとその応用
PCT/JP2009/071553 WO2010074209A1 (fr) 2008-12-26 2009-12-25 Lipase mutante et utilisation associée

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2008331771A JP5574400B2 (ja) 2008-12-26 2008-12-26 変異型リパーゼとその応用

Publications (2)

Publication Number Publication Date
JP2012044869A JP2012044869A (ja) 2012-03-08
JP5574400B2 true JP5574400B2 (ja) 2014-08-20

Family

ID=42287814

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2008331771A Expired - Fee Related JP5574400B2 (ja) 2008-12-26 2008-12-26 変異型リパーゼとその応用

Country Status (2)

Country Link
JP (1) JP5574400B2 (fr)
WO (1) WO2010074209A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101500073B1 (ko) * 2013-04-23 2015-03-06 씨제이제일제당 (주) L-아르기닌 생산능이 향상된 코리네박테리움속 미생물 및 이를 이용한 l-아르기닌의 생산 방법
CN109161538B (zh) * 2018-09-29 2021-10-15 云南师范大学 一种热稳性提高的脂肪酶突变体及其应用
CN109182299B (zh) * 2018-10-30 2021-12-31 福建师范大学 耐受过氧化氢的枯草芽胞杆菌脂肪酶突变体及其制备方法
CN109929765B (zh) * 2019-03-20 2020-10-13 武汉大学 一株隐球酵母及其胞外多糖与应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3507860B2 (ja) * 2000-03-13 2004-03-15 独立行政法人酒類総合研究所 酵母由来の新規リパーゼｃｓ２
JP4085157B2 (ja) * 2002-08-20 2008-05-14 独立行政法人酒類総合研究所 リパーゼｃｓ２遺伝子
US7547531B2 (en) * 2005-01-18 2009-06-16 Ajinomoto Co., Inc. L-amino acid producing microorganism which has been modified to inactive the fimH gene, and a method for producing I-amino acid

Also Published As

Publication number Publication date
WO2010074209A1 (fr) 2010-07-01
JP2012044869A (ja) 2012-03-08

Similar Documents

Publication Publication Date Title
KR101186128B1 (ko) Ｌ-아미노산 생산 세균 및 ｌ-아미노산의 생산 방법
ES2238773T3 (es) Procedimiento para la produccion microbiana de aminoacidos de las familias del aspartato y/o glutamato, y agentes utilizables en el procedimiento.
CN101374953B (zh) 用于产生l-氨基酸的方法
CN101111602B (zh) 生产碱性物质的方法
KR100930842B1 (ko) Ｌ-글루탐산 생산 미생물 및 ｌ-글루탐산의 제조방법
CN101939412B (zh) 生产氨基酸的微生物以及氨基酸的生产方法
US7205132B2 (en) L-glutamic acid-producing microorganism and a method for producing L-glutamic acid
JP2012165765A (ja) Ｌ−グルタミン酸生産菌及びｌ−グルタミン酸の製造方法
CN101631849A (zh) L-谷氨酸类氨基酸生产微生物以及氨基酸的生产方法
WO2000018935A1 (fr) Procede de construction d'une bacterie produisant des acides amines, et procede de production d'acides amines par une technique de fermentation utilisant ladite bacterie
RU2759956C1 (ru) Микроорганизм, продуцирующий путресцин или орнитин, и способ получения путресцина или орнитина с использованием этого микроорганизма
JP2011067095A (ja) 発酵法による目的物質の製造法
EP2471942B1 (fr) Procédé pour la production d' un acide l-aminé
KR20130082478A (ko) 퓨트레신 생산능이 향상된 재조합 미생물 및 이를 이용하여 퓨트레신을 생산하는 방법
JP6582997B2 (ja) 変異型グルタミン酸−システインリガーゼ、及び、γ−グルタミルバリルグリシンの製造法
JP5574400B2 (ja) 変異型リパーゼとその応用
JP2007097573A (ja) Ｌ−グルタミン酸生産菌及びｌ−グルタミン酸の製造方法
KR20020073153A (ko) Ｌ-아미노산의 제조법 및 신규 유전자
WO2007024010A1 (fr) Bactérie produisant de l’acide l-glutamique et procédé de production de l’acide l-glutamique
EP1424397A1 (fr) Méthode de production de L-glutamine et bactérie produisant de la L-glutamine
WO2017146188A1 (fr) Procédé de production d'un acide l-aminé
JP2008283863A (ja) Ｌ−アミノ酸生産菌及びｌ−アミノ酸の製造方法
WO2004113550A1 (fr) Procede de production d'acide l-glutamique
JP2012196144A (ja) ビブリオ属細菌を用いたｌ−リジンの製造法
JP2017046673A (ja) γ−グルタミルバリン合成酵素、及び、γ−グルタミルバリルグリシンの製造法

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20111226

A521 Written amendment

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20120227

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20131001

A521 Written amendment

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20131126

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20131126

A521 Written amendment

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20131126

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20140304

A521 Written amendment

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140424

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20140603

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20140626

R150 Certificate of patent or registration of utility model

Ref document number: 5574400

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

LAPS Cancellation because of no payment of annual fees