JP5461643B2 - ヒト抗体及びタンパク質 - Google Patents
ヒト抗体及びタンパク質 Download PDFInfo
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- JP5461643B2 JP5461643B2 JP2012198433A JP2012198433A JP5461643B2 JP 5461643 B2 JP5461643 B2 JP 5461643B2 JP 2012198433 A JP2012198433 A JP 2012198433A JP 2012198433 A JP2012198433 A JP 2012198433A JP 5461643 B2 JP5461643 B2 JP 5461643B2
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
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Description
(1)その他の抗体可変領域の範囲からアミノ酸配列のセグメントを結合することによって抗体可変領域遺伝子を準備して、様々な可変領域遺伝子のライブラリを生成するステップと、
(2)抗体可変領域遺伝子のライブラリを発現ベクタ内へクローニングするステップと、
(3)抗体可変領域のライブラリをスクリーニングして、所望の特性を有するライブラリの員を回復するステップと、
を具える修飾抗体の生成方法を提供する。
(1)合成VH及びVL遺伝子を設計するステップと、
(2)合成VH及びVL遺伝子をクローニングするステップと、
(3)合成VH及びVL遺伝子を発現するステップと、
(4)所望の特性を有する複合抗体をスクリーニングして選択するステップと、
(5)リード複合抗体を最適化するステップと、
(6)(選択的に)T細胞エピトープを回避するステップと、
を含む。
(1)1又はそれ以上の参照抗体の配列を分析するステップと、
(2)合成VH及びVL遺伝子を設計するステップと、
(3)(選択的に)T細胞エピトープを回避するステップと、
(4)合成VH及びVL遺伝子をクローニングするステップと、
(5)合成VH及びVL遺伝子を発現するステップと、
(6)所望の特性を有する複合抗体をスクリーニングして選択するステップと、
(7)T細胞エピトープの最適な回避を含むリード複合抗体を最適化するステップと、
を含む。
(1)T細胞エピトープの選択的分析を含む1又はそれ以上の参照タンパク質の配列分析をするステップと、
(2)合成タンパク質遺伝子を設計するステップと、
(3)T細胞エピトープを(選択的に)回避するステップと、
(4)合成タンパク質遺伝子をクローニングするステップと、
(5)合成タンパク質遺伝子を発現させるステップと、
(6)スクリーニングを行い、所望の特性を有する合成タンパク質を選択するステップと、
(7)T細胞エピトープの選択的回避を含むリード合成タンパク質を最適化するステップ。
SEQ.ID.No.5は、SEQ.ID.No.3に対して、次の変化を含む:
T82aN+R83K
T82aN+R83K+D82bS
T82aN+R83K+D82bS+V32A
T82aN+R83K+D82bS+V32A+V78A
SEQ.ID.No.9は、SEQ.ID.No.4に対して、次の変化を含む:
I10T+N103R
I10T+N103R+S80A
I10T+N103R+S80AN41D
Claims (3)
- ヘルパーT細胞を回避するように複合抗体の可変領域又は抗原結合フラグメントをスクリーニングする方法であって、当該方法が:
(a)2ないし31アミノ酸長のアミノ酸配列の2以上のペプチドから得られた、複合抗体の可変領域又は抗原結合フラグメントをコードする遺伝子のライブラリを、別の抗体又は抗原結合フラグメントから生成するステップであって、前記2以上のペプチドが完全なCDRでも完全なフレームワーク領域でもないステップと;
(b)ヘルパーT細胞を回避するように抗体の可変領域又は抗原結合フラグメントをスクリーニングするステップと;
(c)1以上の対象の抗原に結合させるべく抗体の可変領域又は抗原結合フラグメントのライブラリを発現し、スクリーニングするステップと;
を具えることを特徴とする方法。 - 請求項1に記載の方法において、前記ライブラリを生成するのに用いられる前記2以上のペプチドがヒト抗体から得られることを特徴とする方法。
- 請求項1又は2に記載の方法において、該抗体の可変領域又は抗原結合フラグメントコードする遺伝子が1以上の制御性T細胞のエピトープの挿入又は追加を通して更に変更されることを特徴とする方法。
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JP6054630B2 (ja) * | 2012-05-18 | 2016-12-27 | ヤマト包装技術研究所株式会社 | 物品拘束具、ロールボックスパレット及び物品の固定方法 |
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US20170210820A1 (en) | 2017-07-27 |
KR101385886B1 (ko) | 2014-04-24 |
DK1844074T3 (da) | 2013-07-15 |
EP1844074B1 (en) | 2013-04-24 |
ES2417133T3 (es) | 2013-08-06 |
JP2013028616A (ja) | 2013-02-07 |
EP1844074A2 (en) | 2007-10-17 |
CN101115771A (zh) | 2008-01-30 |
KR20070107739A (ko) | 2007-11-07 |
JP2016210782A (ja) | 2016-12-15 |
JP2008528668A (ja) | 2008-07-31 |
WO2006082406A3 (en) | 2007-02-01 |
JP2014073128A (ja) | 2014-04-24 |
US20080206239A1 (en) | 2008-08-28 |
WO2006082406A2 (en) | 2006-08-10 |
EP2388271A2 (en) | 2011-11-23 |
EP2388271A3 (en) | 2012-08-01 |
CA2596833A1 (en) | 2006-08-10 |
US20150031860A1 (en) | 2015-01-29 |
AU2006210724A1 (en) | 2006-08-10 |
HK1109636A1 (en) | 2008-06-13 |
US20150031550A1 (en) | 2015-01-29 |
CN101115771B (zh) | 2013-06-05 |
CA2596833C (en) | 2016-04-12 |
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