JP5415760B2 - 生体標本及びその作製方法 - Google Patents
生体標本及びその作製方法 Download PDFInfo
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- JP5415760B2 JP5415760B2 JP2008526829A JP2008526829A JP5415760B2 JP 5415760 B2 JP5415760 B2 JP 5415760B2 JP 2008526829 A JP2008526829 A JP 2008526829A JP 2008526829 A JP2008526829 A JP 2008526829A JP 5415760 B2 JP5415760 B2 JP 5415760B2
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- aromatic hydrocarbon
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- 238000004519 manufacturing process Methods 0.000 title claims description 6
- 239000007850 fluorescent dye Substances 0.000 claims description 92
- 125000000217 alkyl group Chemical group 0.000 claims description 84
- 125000002029 aromatic hydrocarbon group Chemical group 0.000 claims description 73
- 125000006850 spacer group Chemical group 0.000 claims description 53
- 125000003118 aryl group Chemical group 0.000 claims description 51
- -1 halide ion Chemical class 0.000 claims description 51
- 125000000472 sulfonyl group Chemical group *S(*)(=O)=O 0.000 claims description 42
- 125000000623 heterocyclic group Chemical group 0.000 claims description 41
- 125000000524 functional group Chemical group 0.000 claims description 40
- 125000001424 substituent group Chemical group 0.000 claims description 35
- 239000000975 dye Substances 0.000 claims description 33
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 33
- 150000002460 imidazoles Chemical class 0.000 claims description 33
- 238000000034 method Methods 0.000 claims description 33
- 125000001931 aliphatic group Chemical group 0.000 claims description 32
- 230000005284 excitation Effects 0.000 claims description 31
- 125000005647 linker group Chemical group 0.000 claims description 31
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims description 30
- 125000003545 alkoxy group Chemical group 0.000 claims description 29
- 125000005907 alkyl ester group Chemical group 0.000 claims description 29
- 125000003277 amino group Chemical group 0.000 claims description 29
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims description 28
- 125000003342 alkenyl group Chemical group 0.000 claims description 27
- 125000004185 ester group Chemical group 0.000 claims description 24
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical group C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 claims description 23
- 125000004432 carbon atom Chemical group C* 0.000 claims description 23
- 239000000758 substrate Substances 0.000 claims description 23
- 125000005843 halogen group Chemical group 0.000 claims description 20
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 19
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- 125000004093 cyano group Chemical group *C#N 0.000 claims description 19
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- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 claims description 16
- 238000002372 labelling Methods 0.000 claims description 16
- JYEUMXHLPRZUAT-UHFFFAOYSA-N 1,2,3-triazine Chemical group C1=CN=NN=C1 JYEUMXHLPRZUAT-UHFFFAOYSA-N 0.000 claims description 13
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- 239000010452 phosphate Substances 0.000 claims description 13
- RAXXELZNTBOGNW-UHFFFAOYSA-N 1H-imidazole Chemical group C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 claims description 9
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- IQPQWNKOIGAROB-UHFFFAOYSA-N isocyanate group Chemical group [N-]=C=O IQPQWNKOIGAROB-UHFFFAOYSA-N 0.000 claims description 9
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- GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical group C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 claims description 8
- NQRYJNQNLNOLGT-UHFFFAOYSA-N Piperidine Chemical group C1CCNCC1 NQRYJNQNLNOLGT-UHFFFAOYSA-N 0.000 claims description 8
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- 125000001302 tertiary amino group Chemical group 0.000 claims description 8
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- 229910052711 selenium Inorganic materials 0.000 claims description 7
- BCMCBBGGLRIHSE-UHFFFAOYSA-N 1,3-benzoxazole Chemical group C1=CC=C2OC=NC2=C1 BCMCBBGGLRIHSE-UHFFFAOYSA-N 0.000 claims description 6
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- 125000004434 sulfur atom Chemical group 0.000 claims description 6
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- 238000006243 chemical reaction Methods 0.000 description 27
- QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 description 26
- 210000004027 cell Anatomy 0.000 description 25
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- 229940024606 amino acid Drugs 0.000 description 22
- 150000001413 amino acids Chemical class 0.000 description 22
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 22
- YTPLMLYBLZKORZ-UHFFFAOYSA-N Thiophene Chemical group C=1C=CSC=1 YTPLMLYBLZKORZ-UHFFFAOYSA-N 0.000 description 21
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 18
- NOUIINDKKXZYDN-UHFFFAOYSA-N 2-oxido-4,5-dihydrooxadiazol-2-ium Chemical compound [O-][N+]1=NCCO1 NOUIINDKKXZYDN-UHFFFAOYSA-N 0.000 description 16
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- RWXCVESEMJNNMF-UHFFFAOYSA-N oxadiazolo[5,4-b]pyridine Chemical compound C1=CN=C2ON=NC2=C1 RWXCVESEMJNNMF-UHFFFAOYSA-N 0.000 description 16
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- G01N1/00—Sampling; Preparing specimens for investigation
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US10572996B2 (en) * | 2016-06-28 | 2020-02-25 | Contextvision Ab | Method and system for detecting pathological anomalies in a digital pathology image and method for annotating a tissue slide |
CN106706643B (zh) * | 2017-02-23 | 2019-07-02 | 马青山 | 一种肝癌对比切片检测方法 |
JP7260127B2 (ja) * | 2018-02-09 | 2023-04-18 | 株式会社シバサキ | 細菌検出方法及び細菌検出装置 |
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JPH07278524A (ja) * | 1993-11-12 | 1995-10-24 | Shino Junko | 組織標本作製用の脱水剤を置換するための脂溶性置換剤及び前記脂溶性置換剤を用いた組織標本作製方法 |
JP2005208026A (ja) * | 2003-12-24 | 2005-08-04 | Shinichiro Isobe | 生体分子の検出方法及びそれに用いる標識色素並びに標識キット |
WO2006030788A1 (fr) * | 2004-09-14 | 2006-03-23 | Shinichiro Isobe | Intercaleur et procede de detection de gene utilisant ledit intercaleur |
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JPH06313118A (ja) * | 1993-04-30 | 1994-11-08 | Matsushita Electric Ind Co Ltd | 標識色素及び標識色素の中間物 |
EP1325300A1 (fr) * | 2000-09-15 | 2003-07-09 | Biogenex Laboratories | Amelioration d'hybridation in situ |
JP2006180835A (ja) * | 2004-12-28 | 2006-07-13 | Shinichiro Isobe | 遺伝子検出方法 |
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JPH07278524A (ja) * | 1993-11-12 | 1995-10-24 | Shino Junko | 組織標本作製用の脱水剤を置換するための脂溶性置換剤及び前記脂溶性置換剤を用いた組織標本作製方法 |
JP2005208026A (ja) * | 2003-12-24 | 2005-08-04 | Shinichiro Isobe | 生体分子の検出方法及びそれに用いる標識色素並びに標識キット |
WO2006030788A1 (fr) * | 2004-09-14 | 2006-03-23 | Shinichiro Isobe | Intercaleur et procede de detection de gene utilisant ledit intercaleur |
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