JP5409005B2 - 非ランダムプライマーを用いる核酸増幅 - Google Patents

非ランダムプライマーを用いる核酸増幅 Download PDF

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JP5409005B2
JP5409005B2 JP2008538067A JP2008538067A JP5409005B2 JP 5409005 B2 JP5409005 B2 JP 5409005B2 JP 2008538067 A JP2008538067 A JP 2008538067A JP 2008538067 A JP2008538067 A JP 2008538067A JP 5409005 B2 JP5409005 B2 JP 5409005B2
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population
nucleic acid
oligonucleotides
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subpopulation
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JP2009513142A (ja
JP2009513142A5 (https=
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キヤスル,ジヨン
レイモンド,クリストフアー・ケイ
アーマー,クリストフアー
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ロゼッタ インファーマティックス エルエルシー
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
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  • Molecular Biology (AREA)
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  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2008538067A 2005-10-27 2006-10-27 非ランダムプライマーを用いる核酸増幅 Expired - Fee Related JP5409005B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US73152905P 2005-10-27 2005-10-27
US60/731,529 2005-10-27
US79045806P 2006-04-07 2006-04-07
US60/790,458 2006-04-07
PCT/US2006/042250 WO2007050990A2 (en) 2005-10-27 2006-10-27 Nucleic acid amplification using non-random primers

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JP2009513142A JP2009513142A (ja) 2009-04-02
JP2009513142A5 JP2009513142A5 (https=) 2009-12-17
JP5409005B2 true JP5409005B2 (ja) 2014-02-05

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JP2008538067A Expired - Fee Related JP5409005B2 (ja) 2005-10-27 2006-10-27 非ランダムプライマーを用いる核酸増幅

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US (2) US20080187969A1 (https=)
EP (2) EP2479291A1 (https=)
JP (1) JP5409005B2 (https=)
CA (1) CA2626977A1 (https=)
WO (1) WO2007050990A2 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE602006018352D1 (de) 2005-12-06 2010-12-30 Ambion Inc Rückübertragungs-primer und verfahren zu deren entwurf
CA2680060A1 (en) * 2007-03-07 2008-09-12 Nventa Biopharmaceuticals Corporation Double-stranded locked nucleic acid compositions
WO2009055732A1 (en) * 2007-10-26 2009-04-30 Rosetta Inpharmatics Llc Cdna synthesis using non-random primers
AU2009205523A1 (en) 2008-01-14 2009-07-23 Applied Biosystems, Llc Compositions, methods, and kits for detecting ribonucleic acid
EP2285961A4 (en) * 2008-05-15 2012-10-17 Topigen Pharmaceuticals Inc OLIGONUCLEOTIDES FOR THE TREATMENT OF INFLAMMATION AND NEOPLASTIC CELL REPRODUCTION
US20100159533A1 (en) * 2008-11-24 2010-06-24 Helicos Biosciences Corporation Simplified sample preparation for rna analysis
EP3584324A1 (en) * 2008-11-24 2019-12-25 Sequenom, Inc. Nucleic acid quantification products and processes
EP2475777A4 (en) * 2009-09-11 2013-03-06 Nugen Technologies Inc COMPOSITIONS AND METHODS FOR COMPLETE TRANSCRIPTOMAL ANALYSIS
WO2012064739A2 (en) * 2010-11-08 2012-05-18 The Trustees Of Columbia University In The City Of New York Microbial enrichment primers
US12595500B2 (en) 2011-09-26 2026-04-07 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
CN107058059B (zh) 2011-09-26 2020-08-07 基因技术股份公司 高效的小体积核酸合成
US10407676B2 (en) 2014-12-09 2019-09-10 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US20220251639A1 (en) * 2019-05-20 2022-08-11 Kyushu University, National University Corporation Oligonucleotide, omics analysis method, and omics analysis kit

Family Cites Families (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5723597A (en) * 1983-01-10 1998-03-03 Gen-Probe Incorporated Ribosomal nucleic acid probes for detecting organisms or groups of organisms
US6221581B1 (en) * 1984-04-27 2001-04-24 Enzo Diagnostics, Inc. Processes for detecting polynucleotides, determining genetic mutations or defects in genetic material, separating or isolating nucleic acid of interest from samples, and useful compositions of matter and multihybrid complex compositions
US6150517A (en) * 1986-11-24 2000-11-21 Gen-Probe Methods for making oligonucleotide probes for the detection and/or quantitation of non-viral organisms
US5541308A (en) * 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
US5639604A (en) * 1987-09-21 1997-06-17 Gen-Probe Incorporated Homogeneous protection assay
US5084565A (en) * 1988-08-18 1992-01-28 Gene-Trak Systems Probes for the specific detection of escherichia coli and shigella
US5512439A (en) * 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
US5708154A (en) * 1989-02-24 1998-01-13 City Of Hope RNA-DNA hybrid molecules of nucleic acid
WO1990010717A1 (en) * 1989-03-10 1990-09-20 Gene-Trak Systems Preventing interference with affinity capture schemes
CA2031499A1 (en) * 1989-05-31 1990-12-01 David J. Lane Universal eubacteria nucleic acid probes and methods
US5744101A (en) * 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5545522A (en) * 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
CA2025181A1 (en) * 1989-10-12 1991-04-13 William G. Weisburg Nucleic acid probes and methods for detecting fungi
EP0675966B1 (en) * 1992-02-19 2004-10-06 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
KR100249110B1 (ko) * 1992-05-06 2000-04-01 다니엘 엘. 캐시앙 핵산 서열 증폭 방법, 이에 이용되는 조성물 및 키트
GB9211979D0 (en) * 1992-06-05 1992-07-15 Buchard Ole Uses of nucleic acid analogues
US5633134A (en) * 1992-10-06 1997-05-27 Ig Laboratories, Inc. Method for simultaneously detecting multiple mutations in a DNA sample
US5500356A (en) * 1993-08-10 1996-03-19 Life Technologies, Inc. Method of nucleic acid sequence selection
US6309823B1 (en) * 1993-10-26 2001-10-30 Affymetrix, Inc. Arrays of nucleic acid probes for analyzing biotransformation genes and methods of using the same
US5639428A (en) * 1994-07-19 1997-06-17 Becton Dickinson And Company Method and apparatus for fully automated nucleic acid amplification, nucleic acid assay and immunoassay
FR2737502B1 (fr) * 1995-07-31 1997-10-24 Genset Sa Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection
US6013440A (en) * 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
JPH11509427A (ja) * 1996-05-14 1999-08-24 ベーリンガー マンハイム ゲーエムベーハー 試料中の核酸分子の発現の同定方法および/または定量方法
US6203978B1 (en) * 1996-05-31 2001-03-20 Du Vergier Ltd. Capture of single stranded nucleic acids
WO1998012354A1 (en) * 1996-09-19 1998-03-26 Affymetrix, Inc. Identification of molecular sequence signatures and methods involving the same
EP0972830A4 (en) * 1997-02-21 2003-02-05 Takara Shuzo Co GENES ASSOCIATED WITH CANCER
EP0975807B1 (en) * 1997-05-02 2006-09-27 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
EP1007739A2 (en) * 1997-09-05 2000-06-14 Sidney Kimmel Cancer Center Selection of pcr primer pairs to amplify a group of nucleotide sequences
US5932451A (en) * 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
US6270973B1 (en) * 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US6197510B1 (en) * 1998-10-01 2001-03-06 Bio-Id Diagnostic Inc. Multi-loci genomic analysis
US6316193B1 (en) * 1998-10-06 2001-11-13 Origene Technologies, Inc. Rapid-screen cDNA library panels
US6951722B2 (en) * 1999-03-19 2005-10-04 Takara Bio Inc. Method for amplifying nucleic acid sequence
US6582906B1 (en) * 1999-04-05 2003-06-24 Affymetrix, Inc. Proportional amplification of nucleic acids
US6132997A (en) * 1999-05-28 2000-10-17 Agilent Technologies Method for linear mRNA amplification
US6465183B2 (en) * 1999-07-01 2002-10-15 Agilent Technologies, Inc. Multidentate arrays
US6495320B1 (en) * 1999-07-21 2002-12-17 Affymetrix, Inc. Even length proportional amplification of nucleic acids
AU781570B2 (en) * 1999-07-23 2005-06-02 Gen-Probe Incorporated Polynucleotide amplification method
US20020072061A1 (en) * 1999-07-30 2002-06-13 Alex Chenchik Methods and compositions for use in synthesizing nucleic acids
EP1276901A2 (en) * 2000-01-13 2003-01-22 Amsterdam Support Diagnostics B.V. A universal nucleic acid amplification system for nucleic acids in a sample
US20030022318A1 (en) * 2000-01-25 2003-01-30 Epiclone, Inc. Method for thermocycling amplification of nucleic acid sequences and the generation of related peptides thereof
US6235483B1 (en) * 2000-01-31 2001-05-22 Agilent Technologies, Inc. Methods and kits for indirect labeling of nucleic acids
US20010031468A1 (en) * 2000-02-08 2001-10-18 Alex Chenchik Analyte assays employing universal arrays
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US6465219B1 (en) * 2000-08-07 2002-10-15 Genemed Biotechnologies, Inc. Polynucleotide pools enriched in either high-abundance or low-abundance sequences
WO2002044399A2 (en) * 2000-11-28 2002-06-06 Rosetta Inpharmatics, Inc. In vitro transcription method for rna amplification
US6391592B1 (en) * 2000-12-14 2002-05-21 Affymetrix, Inc. Blocker-aided target amplification of nucleic acids
US6448387B1 (en) * 2000-12-18 2002-09-10 Monsanto Technology, Llc Polymeric arrays adapted for high expressing polynucleotides
US20030104432A1 (en) * 2001-07-27 2003-06-05 The Regents Of The University Of California Methods of amplifying sense strand RNA
WO2003050304A1 (en) * 2001-12-08 2003-06-19 Seegene, Inc Annealing control primer system for regulating primer annealing specificity and its applications
EP1572902B1 (en) * 2002-02-01 2014-06-11 Life Technologies Corporation HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES
US20030211483A1 (en) * 2002-05-09 2003-11-13 Schroeder Benjamin G. Methods for the enrichment of low-abundance polynucleotides
US20040248094A1 (en) * 2002-06-12 2004-12-09 Ford Lance P. Methods and compositions relating to labeled RNA molecules that reduce gene expression
GB2406169B (en) * 2002-06-12 2006-11-01 Ambion Inc Methods and compositions relating to labeled rna molecules that reduce gene expression
US20040029275A1 (en) * 2002-08-10 2004-02-12 David Brown Methods and compositions for reducing target gene expression using cocktails of siRNAs or constructs expressing siRNAs
US20050003369A1 (en) * 2002-10-10 2005-01-06 Affymetrix, Inc. Method for depleting specific nucleic acids from a mixture
US20060281092A1 (en) * 2003-07-24 2006-12-14 Tanja Wille Method for the reverse transcription and/or amplification of nucleic acids
US20050059024A1 (en) * 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
US7265448B2 (en) * 2004-01-26 2007-09-04 Marvell World Trade Ltd. Interconnect structure for power transistors
EP1871913A2 (en) * 2005-03-25 2008-01-02 Ambion, Inc. Methods and compositions for depleting abundant rna transcripts
US20060255053A1 (en) * 2005-05-16 2006-11-16 Empire Industrial Corp. Sealable container

Also Published As

Publication number Publication date
WO2007050990A3 (en) 2007-12-13
EP1941058A4 (en) 2010-01-20
JP2009513142A (ja) 2009-04-02
EP2479291A1 (en) 2012-07-25
EP1941058A2 (en) 2008-07-09
US20110294701A1 (en) 2011-12-01
CA2626977A1 (en) 2007-05-03
WO2007050990A2 (en) 2007-05-03
US20080187969A1 (en) 2008-08-07

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