JP5252746B2 - 宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン - Google Patents

宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン Download PDF

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JP5252746B2
JP5252746B2 JP2010532232A JP2010532232A JP5252746B2 JP 5252746 B2 JP5252746 B2 JP 5252746B2 JP 2010532232 A JP2010532232 A JP 2010532232A JP 2010532232 A JP2010532232 A JP 2010532232A JP 5252746 B2 JP5252746 B2 JP 5252746B2
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sequence
protein
laccase
nucleic acid
host cell
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バオ、カイ
ワン、フアミン
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ダニスコ・ユーエス・インク
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/62—DNA sequences coding for fusion proteins
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03002—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
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  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2010532232A 2007-11-01 2008-10-30 宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン Expired - Fee Related JP5252746B2 (ja)

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US98443007P 2007-11-01 2007-11-01
US60/984,430 2007-11-01
PCT/US2008/081721 WO2009058956A1 (en) 2007-11-01 2008-10-30 Signal sequences and co-expressed chaperones for improving protein production in a host cell

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JP2013009289A Division JP2013121354A (ja) 2007-11-01 2013-01-22 宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン

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JP2011502483A JP2011502483A (ja) 2011-01-27
JP2011502483A5 JP2011502483A5 (https=) 2013-03-21
JP5252746B2 true JP5252746B2 (ja) 2013-07-31

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JP2010532232A Expired - Fee Related JP5252746B2 (ja) 2007-11-01 2008-10-30 宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン
JP2013009289A Pending JP2013121354A (ja) 2007-11-01 2013-01-22 宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン
JP2014200112A Withdrawn JP2015027308A (ja) 2007-11-01 2014-09-30 宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン

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JP2014200112A Withdrawn JP2015027308A (ja) 2007-11-01 2014-09-30 宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン

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US (1) US20090221030A1 (https=)
EP (1) EP2203556A1 (https=)
JP (3) JP5252746B2 (https=)
CN (1) CN101842479A (https=)
AU (1) AU2008318644B2 (https=)
BR (1) BRPI0818273A2 (https=)
CA (1) CA2704548A1 (https=)
CO (1) CO6210760A2 (https=)
MX (1) MX2010004325A (https=)
WO (1) WO2009058956A1 (https=)

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CN112409464B (zh) * 2020-11-23 2022-04-22 江南大学 提高枯草芽孢杆菌重组蛋白胞外生产水平的信号肽突变体及应用
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CN113736817B (zh) * 2021-10-08 2023-02-03 枣庄市杰诺生物酶有限公司 一种毕赤酵母中提高碱性脂肪酶分泌效率及酶活的方法
KR20240094203A (ko) * 2022-12-15 2024-06-25 씨제이제일제당 (주) 트리코더마 속 균주 유래 pdi를 이용하여 재조합 단백질을 생산하는 방법
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MX2010004325A (es) 2010-05-20
CA2704548A1 (en) 2009-05-07
CO6210760A2 (es) 2010-10-20
JP2015027308A (ja) 2015-02-12
WO2009058956A1 (en) 2009-05-07
JP2011502483A (ja) 2011-01-27
JP2013121354A (ja) 2013-06-20
AU2008318644B2 (en) 2013-05-02
US20090221030A1 (en) 2009-09-03
AU2008318644A1 (en) 2009-05-07
BRPI0818273A2 (pt) 2014-10-14
EP2203556A1 (en) 2010-07-07
CN101842479A (zh) 2010-09-22

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