JP5245060B2 - 改善された発現特性を示す改変型ペルオキシダーゼ酵素遺伝子、及びそれを使用したペルオキシダーゼの生産方法 - Google Patents
改善された発現特性を示す改変型ペルオキシダーゼ酵素遺伝子、及びそれを使用したペルオキシダーゼの生産方法 Download PDFInfo
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- JP5245060B2 JP5245060B2 JP2012164877A JP2012164877A JP5245060B2 JP 5245060 B2 JP5245060 B2 JP 5245060B2 JP 2012164877 A JP2012164877 A JP 2012164877A JP 2012164877 A JP2012164877 A JP 2012164877A JP 5245060 B2 JP5245060 B2 JP 5245060B2
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- 239000008363 phosphate buffer Substances 0.000 description 1
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- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000008057 potassium phosphate buffer Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- DSKIOWHQLUWFLG-SPIKMXEPSA-N prochlorperazine maleate Chemical compound [H+].[H+].[H+].[H+].[O-]C(=O)\C=C/C([O-])=O.[O-]C(=O)\C=C/C([O-])=O.C1CN(C)CCN1CCCN1C2=CC(Cl)=CC=C2SC2=CC=CC=C21 DSKIOWHQLUWFLG-SPIKMXEPSA-N 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 229940107700 pyruvic acid Drugs 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
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- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
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- 235000011152 sodium sulphate Nutrition 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
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- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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| JP2012164877A JP5245060B2 (ja) | 2011-07-28 | 2012-07-25 | 改善された発現特性を示す改変型ペルオキシダーゼ酵素遺伝子、及びそれを使用したペルオキシダーゼの生産方法 |
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| JP2012164877A JP5245060B2 (ja) | 2011-07-28 | 2012-07-25 | 改善された発現特性を示す改変型ペルオキシダーゼ酵素遺伝子、及びそれを使用したペルオキシダーゼの生産方法 |
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| JP5245060B2 true JP5245060B2 (ja) | 2013-07-24 |
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| JP (1) | JP5245060B2 (fr) |
| WO (1) | WO2013015326A1 (fr) |
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| EP2857511B1 (fr) * | 2012-05-31 | 2018-07-04 | Meiji Seika Pharma Co., Ltd. | Procédé de production d'une protéine recombinante à base de peroxydase de raifort à l'aide de champignons filamenteux |
| JP6461793B2 (ja) * | 2013-08-07 | 2019-01-30 | 東洋紡株式会社 | ムコール属由来のフラビンアデニンジヌクレオチド結合型グルコースデヒドロゲナーゼの生産方法 |
| JP6274502B2 (ja) * | 2013-11-27 | 2018-02-07 | 東洋紡株式会社 | 相同組換えの頻度が増大されたクリプトコッカス属のウラシル要求性の菌株を取得する方法 |
| JP6720433B2 (ja) * | 2015-02-18 | 2020-07-08 | 誠司 仲亀 | イソプレンの製造方法 |
| JP6769615B2 (ja) * | 2017-04-20 | 2020-10-14 | 有限会社オフイスヨコオ | 硝酸性窒素の除去方法及び硝酸性窒素除去剤 |
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| WO2013015326A1 (fr) | 2013-01-31 |
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