JP5186254B2 - アメーバ殺滅剤、及び、アメーバ抑制方法 - Google Patents
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- 241000224489 Amoeba Species 0.000 title claims description 53
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- DMSMPAJRVJJAGA-UHFFFAOYSA-N benzo[d]isothiazol-3-one Chemical compound C1=CC=C2C(=O)NSC2=C1 DMSMPAJRVJJAGA-UHFFFAOYSA-N 0.000 description 1
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- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
- 229910052794 bromium Inorganic materials 0.000 description 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
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Description
メチルイミニオ)エチレンジクロライド]と、を有効成分として含有することを特徴とする循環冷却水用のアメーバ殺滅剤である。
細菌検査用寒天を濃度が1.5%(w/v) となるようにイオン交換水に溶かし、121℃、15分間の滅菌後、径90mmの滅菌シャーレに15〜20mL分注して寒天平板を作製した。次いで、別途に標準寒天平板培地で培養した大腸菌を適当な量採取し、滅菌イオン交換水に懸濁して濃厚な大腸菌懸濁液を作製し、これを60℃で1時間加熱処理した後、滅菌イオン交換水でOD600が0.5となるよう希釈した。そして、この液0.3mLを前記の寒天平板の全面に塗布して、大腸菌が寒天の表面に固定される程度に乾燥させ、大腸菌塗布寒天培地を調製した。
上記で作製した大腸菌塗布寒天培地を用いて2日間培養したアカントアメーバ96(Acanthamoeba 96)の栄養体を滅菌水道水に1mL当たり1.2×104個添加したものを試験水として用いた。
実際に稼働している無処理の冷房用冷却水系水(アメーバ数:1500個/100mL以上)に対し、冷却水中の上記CMIの有効成分濃度が0.5mg/L、POの有効成分濃度が5mg/Lをそれぞれ維持するようにアメーバ抑制処理を行った。処理開始1日後のアメーバ数は2個/100mL未満となり、処理開始1週間後も2個/100mL未満であった。なお、冷却水中のアメーバ数の測定は新版レジオネラ症防止指針(監修 厚生省生活衛生局企画課、発行者:(財団法人)ビル管理教育センター 平成11年11月 初版発行)の95〜98頁に記載の方法に準じて行った(以下同じ)。
冷却水中のCMIの有効成分濃度を5mg/Lに維持することで、微生物制御を行っている、実際に稼働している冷房用冷却水系水(アメーバ数:8800個/100mL)に対し、処理方法を変更して、冷却水中の上記CMIの有効成分濃度が1mg/L、POの有効成分濃度が20mg/Lをそれぞれ維持するようにアメーバ抑制処理を行った。処理開始1日後のアメーバ数は2個/100mL未満となり、処理開始1週間後も2個/100mL未満であった。
冷却水中のPOの有効成分濃度を25mg/Lに維持することで、微生物制御を行っている、実際に稼働している冷房用冷却水系水(アメーバ数:10000個/100mL以上)に対し、処理方法を変更して、保有水に対して上記CMIの有効成分濃度が2mg/L、POの有効成分濃度が20mg/Lとなるように1日1回薬品を添加することでアメーバ抑制処理を行った。処理開始1週間後のアメーバ数は2個/100mL未満となり、処理開始1ヶ月後も2個/100mL未満であった。
Claims (2)
- イソチアゾリン系化合物と、ポリ[オキシエチレン(ジメチルイミニオ)エチレン(ジ
メチルイミニオ)エチレンジクロライド]と、を有効成分として含有することを特徴とする循環冷却水用のアメーバ殺滅剤。 - 請求項1に記載のアメーバ殺滅剤を循環冷却水へ添加することを特徴とする循環冷却水のアメーバ抑制方法。
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JP2013040203A (ja) * | 2012-11-12 | 2013-02-28 | Aquas Corp | アメーバ殺滅剤、及び、アメーバ抑制方法 |
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JP2011212522A (ja) * | 2010-03-31 | 2011-10-27 | Aquas Corp | 水系中のイソチアゾリン系化合物の分解抑制方法、及び、水系の微生物制御方法 |
JP2015189741A (ja) * | 2014-03-28 | 2015-11-02 | アクアス株式会社 | 殺菌力の持続性を強化した微生物防除剤及び微生物防除方法 |
JP6189244B2 (ja) * | 2014-03-31 | 2017-08-30 | アクアス株式会社 | 微生物防除剤組成物 |
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ZA894985B (en) * | 1988-11-04 | 1990-04-25 | Buckman Labor Inc | A synergistic combination for use in controlling fungal or bacterial growth in synthetic metalworking fluids |
US5681862A (en) * | 1993-03-05 | 1997-10-28 | Buckman Laboratories International, Inc. | Ionene polymers as microbicides |
JPH11319847A (ja) * | 1997-06-13 | 1999-11-24 | Aquas Corp | 水系中のアメ―バの抑制方法 |
JPH1171208A (ja) * | 1997-08-27 | 1999-03-16 | Sagami Chem Res Center | 殺微生物活性を有する組成物および微生物の制御方法 |
JP2986769B2 (ja) * | 1997-11-27 | 1999-12-06 | アクアス株式会社 | 水系中でアメーバと共存しているレジオネラ属細菌の除菌方法 |
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JP2013040203A (ja) * | 2012-11-12 | 2013-02-28 | Aquas Corp | アメーバ殺滅剤、及び、アメーバ抑制方法 |
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