JP5149184B2 - ゲノムdnaの検出、分析、および種名を同定のための方法と分子診断装置 - Google Patents
ゲノムdnaの検出、分析、および種名を同定のための方法と分子診断装置 Download PDFInfo
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- JP5149184B2 JP5149184B2 JP2008529337A JP2008529337A JP5149184B2 JP 5149184 B2 JP5149184 B2 JP 5149184B2 JP 2008529337 A JP2008529337 A JP 2008529337A JP 2008529337 A JP2008529337 A JP 2008529337A JP 5149184 B2 JP5149184 B2 JP 5149184B2
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20230069081A1 (en) * | 2021-08-30 | 2023-03-02 | Taiwan Semiconductor Manufacturing Co., Ltd. | Method and system for adjusting the gap between a wafer and a top plate in a thin-film deposition process |
Families Citing this family (87)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10533998B2 (en) | 2008-07-18 | 2020-01-14 | Bio-Rad Laboratories, Inc. | Enzyme quantification |
| US7968287B2 (en) | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
| CN101300352B (zh) * | 2005-09-01 | 2013-03-20 | 佳能美国生命科学公司 | 检测、分析和鉴定基因组dna的方法和分子诊断装置 |
| EP3913375A1 (en) | 2006-01-11 | 2021-11-24 | Bio-Rad Laboratories, Inc. | Microfluidic devices and methods of use in the formation and control of nanoreactors |
| US7598091B2 (en) * | 2006-04-04 | 2009-10-06 | Micropoint Bioscience, Inc. | Micromachined diagnostic device with controlled flow of fluid and reaction |
| EP2021113A2 (en) | 2006-05-11 | 2009-02-11 | Raindance Technologies, Inc. | Microfluidic devices |
| US9562837B2 (en) | 2006-05-11 | 2017-02-07 | Raindance Technologies, Inc. | Systems for handling microfludic droplets |
| US7745129B1 (en) * | 2006-07-12 | 2010-06-29 | Kenneth David Schatz | Methods for sequencing of a necleic acid |
| US8148159B2 (en) * | 2006-10-05 | 2012-04-03 | Massachusetts Institute Of Technology | System and methods for stretching polynucleotides |
| US20080167198A1 (en) * | 2007-01-04 | 2008-07-10 | Christopher Gerard Cooney | Filter based detection system |
| US8772046B2 (en) | 2007-02-06 | 2014-07-08 | Brandeis University | Manipulation of fluids and reactions in microfluidic systems |
| WO2008130623A1 (en) | 2007-04-19 | 2008-10-30 | Brandeis University | Manipulation of fluids, fluid components and reactions in microfluidic systems |
| EP2152893B1 (en) * | 2007-06-11 | 2014-02-26 | Wako Pure Chemical Industries, Ltd. | Method for nucleic acid amplification using a microchip PCR with integrated real-time CE detection |
| EP2195446A4 (en) * | 2007-09-17 | 2011-06-22 | Red Ivory Llc | Self-activating signal-producing detection devices and methods |
| CL2008003007A1 (es) * | 2007-10-12 | 2009-10-02 | Bigtec Private Ltd | Un micro chip con capas de ceramicas de coccion conjunta a baja temperatura (ltcc) formando una camara de reaccion, anillos conductores que rodean a la camara de reaccion y un calentador que suministra calor a los anillos, el metodo de fabricacion del micro chip y un micro dispositivo de reaccion en cadena de polimerasa (pcr). |
| US9114397B2 (en) * | 2007-10-25 | 2015-08-25 | Canon U.S. Life Sciences, Inc. | Method of reducing cross-contamination in continuous amplification reactions in a channel |
| EP2212437A4 (en) * | 2007-11-07 | 2011-09-28 | Univ British Columbia | MICROFLUIDIC DEVICE AND METHOD OF USING THE DEVICE |
| US12038438B2 (en) | 2008-07-18 | 2024-07-16 | Bio-Rad Laboratories, Inc. | Enzyme quantification |
| WO2010009365A1 (en) | 2008-07-18 | 2010-01-21 | Raindance Technologies, Inc. | Droplet libraries |
| RU2380418C1 (ru) * | 2008-10-01 | 2010-01-27 | Учреждение Российской академии наук Институт молекулярной биологии им. В.А. Энгельгардта РАН | Сменный микрофлюидный модуль для автоматизированного выделения и очистки нуклеиновых кислот из биологических образцов и способ выделения и очистки нуклеиновых кислот с его использованием |
| WO2010111231A1 (en) | 2009-03-23 | 2010-09-30 | Raindance Technologies, Inc. | Manipulation of microfluidic droplets |
| WO2010111265A1 (en) | 2009-03-24 | 2010-09-30 | University Of Chicago | Slip chip device and methods |
| US9464319B2 (en) | 2009-03-24 | 2016-10-11 | California Institute Of Technology | Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes |
| US9447461B2 (en) | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
| US10196700B2 (en) | 2009-03-24 | 2019-02-05 | University Of Chicago | Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes |
| WO2010118427A1 (en) | 2009-04-10 | 2010-10-14 | Canon U.S. Life Sciences, Inc. | Fluid interface cartridge for a microfluidic chip |
| WO2010141131A1 (en) | 2009-06-04 | 2010-12-09 | Lockheed Martin Corporation | Multiple-sample microfluidic chip for dna analysis |
| US9399797B2 (en) | 2010-02-12 | 2016-07-26 | Raindance Technologies, Inc. | Digital analyte analysis |
| EP4484577A3 (en) | 2010-02-12 | 2025-03-26 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
| US10351905B2 (en) | 2010-02-12 | 2019-07-16 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
| EP2622103B2 (en) | 2010-09-30 | 2022-11-16 | Bio-Rad Laboratories, Inc. | Sandwich assays in droplets |
| EP2439512A1 (en) * | 2010-10-01 | 2012-04-11 | Aqsens Oy | A device for holding a sample |
| WO2012051529A1 (en) | 2010-10-15 | 2012-04-19 | Lockheed Martin Corporation | Micro fluidic optic design |
| EP2646154A2 (en) * | 2010-11-30 | 2013-10-09 | Quantumdx Group Limited | The design, fabrication and use of a microfluidics multitemperature flexible reaction device |
| GB2486435A (en) * | 2010-12-14 | 2012-06-20 | Morteza Bahrami | Liquid sample receiving apparatus |
| CN105689030A (zh) * | 2011-02-07 | 2016-06-22 | 哈佛学院院长等 | 分裂液滴的系统和方法 |
| WO2012109600A2 (en) | 2011-02-11 | 2012-08-16 | Raindance Technologies, Inc. | Methods for forming mixed droplets |
| US9150852B2 (en) | 2011-02-18 | 2015-10-06 | Raindance Technologies, Inc. | Compositions and methods for molecular labeling |
| US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
| US11198126B2 (en) * | 2011-10-31 | 2021-12-14 | Fluid-Screen, Inc. | Apparatus for pathogen detection |
| US9433943B2 (en) | 2011-11-15 | 2016-09-06 | The Board Of Trustees Of The University Of Illinois | Thermal control of droplets by nanoscale field effect transistors |
| US9475013B2 (en) | 2012-02-08 | 2016-10-25 | President And Fellows Of Harvard College | Droplet formation using fluid breakup |
| US9322054B2 (en) | 2012-02-22 | 2016-04-26 | Lockheed Martin Corporation | Microfluidic cartridge |
| WO2013132645A1 (ja) * | 2012-03-09 | 2013-09-12 | 独立行政法人産業技術総合研究所 | 核酸増幅方法 |
| DE102012205171B3 (de) * | 2012-03-29 | 2013-09-12 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Integriertes Einweg-Chipkartuschensystem für mobile Multiparameteranalysen chemischer und/oder biologischer Substanzen |
| US9551030B2 (en) * | 2012-06-17 | 2017-01-24 | Pacific Biosciences Of California, Inc. | Filter architecture for analytical devices |
| US9366647B2 (en) * | 2013-03-14 | 2016-06-14 | Taiwan Semiconductor Manufacturing Company, Ltd. | Optical detection for bio-entities |
| JP2014176302A (ja) * | 2013-03-13 | 2014-09-25 | Seiko Epson Corp | 核酸増幅反応装置及び核酸増幅方法 |
| US9718056B2 (en) | 2013-03-15 | 2017-08-01 | Syracuse University | Microfluidics polymerase chain reaction and high resolution melt detection |
| ES2583413T3 (es) * | 2013-05-22 | 2016-09-20 | Imec Vzw | Dispositivo compacto de análisis de fluido y método de fabricación |
| EP3052236B1 (en) * | 2013-09-30 | 2021-07-14 | Bio-Rad Laboratories, Inc. | Microfluidic cartridge device and methods of use and assembly |
| US11901041B2 (en) | 2013-10-04 | 2024-02-13 | Bio-Rad Laboratories, Inc. | Digital analysis of nucleic acid modification |
| US9944977B2 (en) | 2013-12-12 | 2018-04-17 | Raindance Technologies, Inc. | Distinguishing rare variations in a nucleic acid sequence from a sample |
| US10196678B2 (en) | 2014-10-06 | 2019-02-05 | ALVEO Technologies Inc. | System and method for detection of nucleic acids |
| US9506908B2 (en) | 2014-10-06 | 2016-11-29 | Alveo Technologies, Inc. | System for detection of analytes |
| US10627358B2 (en) | 2014-10-06 | 2020-04-21 | Alveo Technologies, Inc. | Method for detection of analytes |
| US10352899B2 (en) | 2014-10-06 | 2019-07-16 | ALVEO Technologies Inc. | System and method for detection of silver |
| US9921182B2 (en) | 2014-10-06 | 2018-03-20 | ALVEO Technologies Inc. | System and method for detection of mercury |
| US20160116409A1 (en) * | 2014-10-28 | 2016-04-28 | Omnivision Technologies, Inc. | Color-Sensitive Image Sensor With Embedded Microfluidics And Associated Methods |
| US11371091B2 (en) | 2015-06-22 | 2022-06-28 | Fluxergy, Inc. | Device for analyzing a fluid sample and use of test card with same |
| US10519493B2 (en) | 2015-06-22 | 2019-12-31 | Fluxergy, Llc | Apparatus and method for image analysis of a fluid sample undergoing a polymerase chain reaction (PCR) |
| US10214772B2 (en) | 2015-06-22 | 2019-02-26 | Fluxergy, Llc | Test card for assay and method of manufacturing same |
| EP3325153B1 (en) * | 2015-07-17 | 2020-03-18 | Cue Health Inc. | Sample analysis cartridge |
| US10647981B1 (en) | 2015-09-08 | 2020-05-12 | Bio-Rad Laboratories, Inc. | Nucleic acid library generation methods and compositions |
| WO2017119904A1 (en) * | 2016-01-08 | 2017-07-13 | Hewlett-Packard Development Company, L.P. | Polymerase chain reaction device including ejection nozzles |
| CN108883417A (zh) * | 2016-01-20 | 2018-11-23 | 特里夫科技公司 | 即时核酸扩增及检测 |
| US11060959B2 (en) * | 2016-06-03 | 2021-07-13 | Purdue Research Foundation | Systems and methods for analyzing an analyte extracted from a sample using an adsorbent material |
| KR102461735B1 (ko) | 2016-09-23 | 2022-11-01 | 알베오 테크놀로지스 인크. | 분석물을 검출하기 위한 방법 및 조성물 |
| CN107151700B (zh) * | 2017-06-08 | 2023-11-07 | 杭州遂真生物技术有限公司 | 一种基因检测方法及基因检测试剂盒和基因检测设备 |
| CN107527595B (zh) * | 2017-09-27 | 2019-06-07 | 京东方科技集团股份有限公司 | 一种微流控系统及其驱动方法 |
| US12071657B2 (en) | 2017-10-20 | 2024-08-27 | Hewlett-Packard Development Company, L.P. | Nucleic acid amplification |
| WO2020018074A1 (en) | 2018-07-17 | 2020-01-23 | Hewlett-Packard Development Company, L.P. | Droplet ejectors to provide fluids to droplet ejectors |
| US11931738B2 (en) | 2018-04-24 | 2024-03-19 | Hewlett-Packard Development Company, L.P. | Sequenced droplet ejection to deliver fluids |
| WO2019209273A1 (en) | 2018-04-24 | 2019-10-31 | Hewlett-Packard Development Company, L.P. | Microfluidic devices |
| WO2020018073A1 (en) | 2018-07-17 | 2020-01-23 | Hewlett-Packard Development Company, L.P. | Droplet ejectors with target media |
| GB201812192D0 (en) | 2018-07-26 | 2018-09-12 | Ttp Plc | Variable temperature reactor, heater and control circuit for the same |
| AU2019401601A1 (en) | 2018-12-20 | 2021-07-15 | Alveo Technologies, Inc. | Handheld impedance-based diagnostic test system for detecting analytes |
| EP3902632A1 (en) * | 2018-12-28 | 2021-11-03 | Astraveus | Device and method for handling a particle suspension |
| US11195864B2 (en) | 2019-03-01 | 2021-12-07 | Omnivision Technologies, Inc. | Flip-chip sample imaging devices with self-aligning lid |
| WO2020185200A1 (en) * | 2019-03-08 | 2020-09-17 | Hewlett-Packard Development Company, L.P. | Disposable microfluidic cassettes |
| EP4600661A2 (en) * | 2019-09-09 | 2025-08-13 | Lumacyte LLC | Microfluidic devices and method for sampling and analysis of cells using optical forces and raman spectroscopy |
| US20210205813A1 (en) * | 2019-12-23 | 2021-07-08 | National Technology & Engineering Solutions Of Sandia, Llc | Contactless liquid loading to microfluidic devices |
| WO2022010461A1 (en) * | 2020-07-07 | 2022-01-13 | Hewlett-Packard Development Company, L.P. | Microfluidic sensing |
| US12472492B2 (en) | 2020-08-14 | 2025-11-18 | Alveo Technologies, Inc. | Systems and methods of sample depositing and testing |
| KR102416335B1 (ko) * | 2021-06-29 | 2022-07-05 | 에스디바이오센서 주식회사 | 기체 이동 통로와 추출액 이동 통로를 갖는 증폭 모듈 |
| CN113552379A (zh) * | 2021-08-24 | 2021-10-26 | 中国科学院上海微系统与信息技术研究所 | 一种多通道液滴采样装置、包括其的光学分析系统以及方法 |
| DE102023129147A1 (de) | 2023-10-24 | 2025-04-24 | Technische Universität Dresden, Körperschaft des öffentlichen Rechts | Fluidische Vorrichtung und Verfahren zur Probenvorbereitung mit einer fluidischen Vorrichtung |
Family Cites Families (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1127227A (en) | 1977-10-03 | 1982-07-06 | Ichiro Endo | Liquid jet recording process and apparatus therefor |
| US4330787A (en) | 1978-10-31 | 1982-05-18 | Canon Kabushiki Kaisha | Liquid jet recording device |
| US4345262A (en) | 1979-02-19 | 1982-08-17 | Canon Kabushiki Kaisha | Ink jet recording method |
| US4463359A (en) | 1979-04-02 | 1984-07-31 | Canon Kabushiki Kaisha | Droplet generating method and apparatus thereof |
| US4313124A (en) | 1979-05-18 | 1982-01-26 | Canon Kabushiki Kaisha | Liquid jet recording process and liquid jet recording head |
| US4558333A (en) * | 1981-07-09 | 1985-12-10 | Canon Kabushiki Kaisha | Liquid jet recording head |
| JPS59123670A (ja) | 1982-12-28 | 1984-07-17 | Canon Inc | インクジエツトヘツド |
| JPS59138461A (ja) | 1983-01-28 | 1984-08-08 | Canon Inc | 液体噴射記録装置 |
| US5714380A (en) * | 1986-10-23 | 1998-02-03 | Amoco Corporation | Closed vessel for isolating target molecules and for performing amplification |
| US6001229A (en) * | 1994-08-01 | 1999-12-14 | Lockheed Martin Energy Systems, Inc. | Apparatus and method for performing microfluidic manipulations for chemical analysis |
| US6168948B1 (en) * | 1995-06-29 | 2001-01-02 | Affymetrix, Inc. | Miniaturized genetic analysis systems and methods |
| US5885470A (en) * | 1997-04-14 | 1999-03-23 | Caliper Technologies Corporation | Controlled fluid transport in microfabricated polymeric substrates |
| GB9608644D0 (en) * | 1996-04-26 | 1996-07-03 | Scient Genarics Ltd | Nucleic acid manipulation |
| NZ333346A (en) | 1996-06-28 | 2000-03-27 | Caliper Techn Corp | High-throughput screening assay systems in microscale fluidic devices |
| US6221654B1 (en) * | 1996-09-25 | 2001-04-24 | California Institute Of Technology | Method and apparatus for analysis and sorting of polynucleotides based on size |
| JP4274561B2 (ja) * | 1997-08-01 | 2009-06-10 | キヤノン株式会社 | プローブアレイとその製造方法 |
| JP4313861B2 (ja) | 1997-08-01 | 2009-08-12 | キヤノン株式会社 | プローブアレイの製造方法 |
| US5989402A (en) | 1997-08-29 | 1999-11-23 | Caliper Technologies Corp. | Controller/detector interfaces for microfluidic systems |
| US5965410A (en) * | 1997-09-02 | 1999-10-12 | Caliper Technologies Corp. | Electrical current for controlling fluid parameters in microchannels |
| CN1230440C (zh) | 1997-12-06 | 2005-12-07 | Dna研究创新有限公司 | 核酸的分离 |
| US6914137B2 (en) | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| US6534262B1 (en) * | 1998-05-14 | 2003-03-18 | Whitehead Institute For Biomedical Research | Solid phase technique for selectively isolating nucleic acids |
| US6958392B2 (en) * | 1998-10-09 | 2005-10-25 | Whatman, Inc. | Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products |
| US6500323B1 (en) | 1999-03-26 | 2002-12-31 | Caliper Technologies Corp. | Methods and software for designing microfluidic devices |
| AU2001249071B2 (en) * | 2000-02-23 | 2005-09-08 | Caliper Life Sciences, Inc. | Multi-reservoir pressure control system |
| AU2001275995A1 (en) * | 2000-07-31 | 2002-02-13 | Applera Corporation | Apparatus and method for specific release of captured extension products |
| AU2000274922A1 (en) * | 2000-08-08 | 2002-02-18 | Aviva Biosciences Corporation | Methods for manipulating moieties in microfluidic systems |
| JP4949600B2 (ja) * | 2000-09-14 | 2012-06-13 | カリパー・ライフ・サイエンシズ・インク. | 温度介在反応を行うためのマイクロ流体装置及び方法 |
| WO2002046398A2 (en) * | 2000-11-06 | 2002-06-13 | The University Of Houston System | Nucleic acid separation using immobilized metal affinity chromatography |
| WO2002060754A1 (en) * | 2001-01-29 | 2002-08-08 | Caliper Technologies Corp. | Non-mechanical valves for fluidic systems |
| US20020197622A1 (en) | 2001-01-31 | 2002-12-26 | Mcdevitt John T. | Method and apparatus for the confinement of materials in a micromachined chemical sensor array |
| JP2003014773A (ja) * | 2001-03-28 | 2003-01-15 | Canon Inc | プローブ担体、その製造装置およびその製造方法 |
| CA2462914A1 (en) * | 2001-10-11 | 2003-04-17 | Aviva Biosciences Corporation | Methods, compositions, and automated systems for separating rare cells from fluid samples |
| US20030138819A1 (en) * | 2001-10-26 | 2003-07-24 | Haiqing Gong | Method for detecting disease |
| JP2003180351A (ja) | 2001-12-13 | 2003-07-02 | Adgene Co Ltd | 核酸高次構造誘導を用いた核酸変異検出法 |
| JP2003334082A (ja) | 2002-05-17 | 2003-11-25 | Adgene Co Ltd | 波形生成プライマー、該プライマーを使用した核酸増幅方法及び核酸同定方法 |
| JP2004037112A (ja) * | 2002-06-28 | 2004-02-05 | Canon Inc | 核酸チップ基板、及び核酸チップの検査方法、並びに製造方法 |
| JP3937954B2 (ja) * | 2002-07-18 | 2007-06-27 | キヤノン株式会社 | プローブ担体の製造方法および製造装置 |
| US7217542B2 (en) * | 2002-10-31 | 2007-05-15 | Hewlett-Packard Development Company, L.P. | Microfluidic system for analyzing nucleic acids |
| US20060210984A1 (en) * | 2003-03-03 | 2006-09-21 | Jeremy Lambert | Use of nucleic acid mimics for internal reference and calibration in a flow cell microarray binding assay |
| US20040197780A1 (en) * | 2003-04-02 | 2004-10-07 | Agencourt Bioscience Corporation | Method for isolating nucleic acids |
| JP2005024278A (ja) | 2003-06-30 | 2005-01-27 | Canon Inc | プローブ担体の製造方法及び製造装置 |
| JP2006198523A (ja) * | 2005-01-20 | 2006-08-03 | Fuji Photo Film Co Ltd | 加圧エア供給機構、加圧装置、及び抽出機 |
| US7604938B2 (en) * | 2005-02-18 | 2009-10-20 | Canon U.S. Life Sciences, Inc. | Devices and methods for monitoring genomic DNA of organisms |
| CN101300352B (zh) * | 2005-09-01 | 2013-03-20 | 佳能美国生命科学公司 | 检测、分析和鉴定基因组dna的方法和分子诊断装置 |
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2006
- 2006-09-01 CN CN2006800405779A patent/CN101300352B/zh active Active
- 2006-09-01 WO PCT/US2006/034336 patent/WO2007028084A2/en not_active Ceased
- 2006-09-01 JP JP2008529337A patent/JP5149184B2/ja active Active
- 2006-09-01 US US11/514,156 patent/US7915030B2/en active Active
- 2006-09-01 EP EP06802860.4A patent/EP1941035B1/en active Active
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- 2011-03-28 US US13/073,318 patent/US9987627B2/en active Active
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20230069081A1 (en) * | 2021-08-30 | 2023-03-02 | Taiwan Semiconductor Manufacturing Co., Ltd. | Method and system for adjusting the gap between a wafer and a top plate in a thin-film deposition process |
| US12266579B2 (en) * | 2021-08-30 | 2025-04-01 | Taiwan Semiconductor Manufacturing Co., Ltd. | Method and system for adjusting the gap between a wafer and a top plate in a thin-film deposition process |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101300352B (zh) | 2013-03-20 |
| US7915030B2 (en) | 2011-03-29 |
| WO2007028084A3 (en) | 2007-09-13 |
| US20180311661A1 (en) | 2018-11-01 |
| WO2007028084A2 (en) | 2007-03-08 |
| US20110262316A1 (en) | 2011-10-27 |
| JP2009506775A (ja) | 2009-02-19 |
| EP1941035A2 (en) | 2008-07-09 |
| CN101300352A (zh) | 2008-11-05 |
| US10814321B2 (en) | 2020-10-27 |
| EP1941035B1 (en) | 2017-11-22 |
| US9987627B2 (en) | 2018-06-05 |
| US20070111303A1 (en) | 2007-05-17 |
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