JP5065370B2 - 高純度リゾホスファチジルイノシトール及び糖脂質の製造方法 - Google Patents
高純度リゾホスファチジルイノシトール及び糖脂質の製造方法 Download PDFInfo
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- JP5065370B2 JP5065370B2 JP2009291764A JP2009291764A JP5065370B2 JP 5065370 B2 JP5065370 B2 JP 5065370B2 JP 2009291764 A JP2009291764 A JP 2009291764A JP 2009291764 A JP2009291764 A JP 2009291764A JP 5065370 B2 JP5065370 B2 JP 5065370B2
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- phospholipid
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- phospholipids
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- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 238000010898 silica gel chromatography Methods 0.000 description 1
- 239000002195 soluble material Substances 0.000 description 1
- 150000003408 sphingolipids Chemical group 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
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Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
ペースト状大豆リン脂質(リン脂質含量62%)300gに水を加え50%水溶液とし、三共株式会社製ホスホリパーゼA1(11,900ユニット/g)0.2gを加え50℃で攪拌し続け、酸価の上昇に伴う構成リン脂質の変化を辿った図2の数値を表1に示す。一方比較対照例としてホスホリパーゼA2(ノボ・ノルディスク社製、レシターゼ10L 11,000ユニット/ml)0.2mlを加え同様に反応した。結果を表1に示す。
500gのペースト状大豆リン脂質(リン脂質含量62%)に2Lのエタノールを加え攪拌し、室温で放置後エタノール層を傾斜法で分離した後、残渣に対し2度同じ操作を繰り返した。エタノール層をすべて合わせ溶媒を留去し、エタノール可溶部175g(リン脂質含量51.0%)を、又残渣部からエタノール不溶部320g(リン脂質含量65.8%)を得た。エタノール不溶部320gに三共株式会社製ホスホリパーゼA1(11,900ユニット/g)0.25gを溶解した水20mlを加え50℃で攪拌を続け、実施例1と同様反応を行った。結果を表2に示す。
ペースト状のコーンリン脂質(リン脂質含量61.5%)300gに三共株式会社製ホスホリパーゼA1(11,900ユニット/g)0.1gを溶解した水20mlを加え、50℃で撹拌を続け、4日後にホスホリパーゼA1 0.1gを溶解した水20mlを追加した。酸価100に達した時点(反応開始から100時間)でTLC分析をしたところ、原点であるOR以外にリン脂質のスポットが見られなかった。85℃、30分加熱し酵素を失活させ、減圧濃縮した乾燥物を1.2Lのアセトンで5回抽出し、粉末状の脱脂物132gを得た。この粉末状の脱脂物を熱エタノール500mlで3回抽出し、熱エタノ−ル可溶物を5℃以下で放置後、濾過し沈殿物と濾液に分けた。濾液を濃縮乾燥して固形物28.4gを得た。このものをSilicagel 60を用い、クロロホルム:メタノール(80:20)で展開し50%硫酸で発色させたところ、Rf0、0.03、0.53、0.6、0.86、0.91の6スポットを与えた。
PE:ホスファチジルエタノールアミン
PA:ホスファチジン酸
PI:ホスファチジルイノシトール
OR:薄層クロマトグラフ上の原点部
LPC:リゾホスファチジルコリン
LPE:リゾホスファチジルエタノールアミン
LPA:リゾファチジン酸
LPI:リゾホスファチジルイノシトール
Claims (2)
- 含水植物リン脂質にアスペルギルス属の糸状菌が生産するホスホリパーゼA1を作用させ、一次展開溶媒としてクロロホルム:メタノール:7モルのアンモニア水(130:70:8)、二次展開溶媒としてクロロホルム:メタノール:酢酸:ギ酸(50:30:4.5:6.5)を用いるシリカゲル薄層クロマトグラフィー分析で、原点以外にリン脂質のスポットが見られなくなった後に、スフィンゴ糖脂質、ステリルグリコシド、グリセロ糖脂質を得ることを特徴とするスフィンゴ糖脂質、ステリルグリコシド、グリセロ糖脂質の製造法。
- (c)植物リン脂質及び(d)スフィンゴ糖脂質、ステリルグリコシド及びグリセロ糖脂質の1種以上を含有する植物材料を、一次展開溶媒としてクロロホルム:メタノール:7モルのアンモニア水(130:70:8)、二次展開溶媒としてクロロホルム:メタノール:酢酸:ギ酸(50:30:4.5:6.5)を用いるシリカゲル薄層クロマトグラフィー分析で、原点以外にリン脂質のスポットが見られなくなるまでホスホリパーゼA1で加水分解し、反応液から上記(d)成分を採取することを特徴とするスフィンゴ糖脂質、ステリルグリコシド及びグリセロ糖脂質の1種以上の製造方法。
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JPH0662850A (ja) * | 1992-06-16 | 1994-03-08 | Sankyo Co Ltd | ホスホリパーゼa1 |
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JPH07222592A (ja) * | 1993-12-16 | 1995-08-22 | Sankyo Co Ltd | ホスホリパーゼa1によるリゾリン脂質の製造法 |
JPH09227895A (ja) * | 1996-02-20 | 1997-09-02 | Sankyo Co Ltd | 2−アシル−1−リゾリン脂質の用途 |
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WO2022010559A1 (en) * | 2020-07-06 | 2022-01-13 | Kemin Industries, Inc. | A method for the preparation of lysophosphatidylinositol |
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