JP4900587B2 - ヒト胚幹細胞由来の最終分化ドーパミン作動性神経細胞の誘導 - Google Patents

ヒト胚幹細胞由来の最終分化ドーパミン作動性神経細胞の誘導 Download PDF

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JP4900587B2
JP4900587B2 JP2006506517A JP2006506517A JP4900587B2 JP 4900587 B2 JP4900587 B2 JP 4900587B2 JP 2006506517 A JP2006506517 A JP 2006506517A JP 2006506517 A JP2006506517 A JP 2006506517A JP 4900587 B2 JP4900587 B2 JP 4900587B2
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サティシュ マハデオラオ トテイ，
ジータ ラビンドラン，
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リライアンス ライフ サイエンシーズ ピーヴィーティー． リミテッド
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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JP2006506517A 2003-03-12 2004-03-11 ヒト胚幹細胞由来の最終分化ドーパミン作動性神経細胞の誘導 Expired - Fee Related JP4900587B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
IN269/MUM/2003 2003-03-12
IN269MU2003 2003-03-12
US52004803P 2003-11-14 2003-11-14
US60/520,048 2003-11-14
PCT/IB2004/001246 WO2004081172A2 (en) 2003-03-12 2004-03-11 Derivation of terminally differentiated dopaminergic neurons from human embryonic stem cells

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JP2010035457A Division JP2010187669A (ja) 2003-03-12 2010-02-19 ヒト胚幹細胞由来の最終分化ドーパミン作動性神経細胞の誘導

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JP2006521807A JP2006521807A (ja) 2006-09-28
JP2006521807A5 JP2006521807A5 (https=) 2010-04-08
JP4900587B2 true JP4900587B2 (ja) 2012-03-21

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ZA (1) ZA200401646B (https=)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10227563B2 (en) 2008-06-06 2019-03-12 Riken Method for culture of stem cell
CN102257386B (zh) * 2008-10-24 2014-12-31 B.R.A.H.M.S有限公司 通过测定标志肽的水平在已患过中风或短暂性缺血性发作的患者中进行预后和风险评估
CN101824399B (zh) * 2009-03-05 2012-05-30 中日友好医院 从人羊膜上皮细胞中诱导分化出多巴胺能神经元的方法及分离所得神经元的用途
CN101824398B (zh) * 2009-03-05 2012-08-08 中日友好医院 人羊膜上皮细胞与神经干细胞共培养诱导分化多巴胺能神经元的方法
JP5787370B2 (ja) 2009-11-05 2015-09-30 国立研究開発法人理化学研究所 幹細胞の分化誘導方法
WO2011108766A1 (en) * 2010-03-03 2011-09-09 Kyoto University METHOD FOR DIAGNOSING A PROTEIN MISFOLDING DISEASE USING NERVE CELLS DERIVED FROM iPS CELLS
PT2773748T (pt) * 2011-11-04 2020-03-26 Memorial Sloan Kettering Cancer Center Neurónios dopaminérgicos (da) do mesencéfalo para enxerto
JP6300807B2 (ja) * 2012-10-16 2018-03-28 メルツ ファルマ ゲーエムベーハー ウント コンパニー カーゲーアーアー 神経毒素ポリペプチドの生物学的活性の決定のための細胞試験系
AU2014316100B2 (en) * 2013-09-05 2020-11-05 Eisai R&D Management Co., Ltd. New method for inducing dopamine-producing neural precursor cells
ES2972541T3 (es) * 2014-09-08 2024-06-13 Riken Método para producir tejido progenitor cerebeloso
CN105087475B (zh) * 2015-09-17 2018-12-04 广州赛莱拉干细胞科技股份有限公司 一种细胞培养液及其应用以及诱导牙髓干细胞向神经样细胞分化的方法
AU2016335563B2 (en) * 2015-10-08 2022-10-27 Neurona Therapeutics Inc. Neural precursor cell populations and uses thereof
CA3021628A1 (en) 2016-04-22 2017-10-26 Kyoto University Method for producing dopamine-producing neural precursor cells
FR3058892B1 (fr) * 2016-11-23 2021-04-09 Univ Bordeaux Unite de tissu neural et utilisation d'une telle unite pour l'implantation dans le systeme nerveux d'un mammifere
EP3551748B1 (en) * 2016-12-12 2022-07-13 Health and Biotech France (H&B France) Perinatal tissue derived mesenchymal stem cells: method of preparation and uses thereof
JPWO2020054647A1 (ja) * 2018-09-14 2021-11-11 学校法人慶應義塾 アストロサイトの製造方法
CN111304167B (zh) * 2018-12-12 2024-03-26 上海泉眼生物科技有限公司 人源脂肪干细胞来源的神经元前体细胞及其制备方法和应用
CN113439120B (zh) * 2019-01-22 2025-02-14 高丽大学校产学协力团 用于将神经干细胞分化为星形胶质细胞的基于直接细胞转化的方法
CN114606188A (zh) * 2019-03-06 2022-06-10 安徽中盛溯源生物科技有限公司 一种中脑多巴胺能神经前体细胞的制备方法及其应用
CN110257332A (zh) * 2019-07-08 2019-09-20 广东省赛莱拉干细胞研究院 一种诱导间充质干细胞分化成为多巴胺能神经元的培养基以及方法
CN110669143B (zh) * 2019-10-10 2021-05-14 中国人民解放军军事科学院军事医学研究院 一种短肽及其应用
CN114657119B (zh) * 2022-03-16 2023-10-20 广东省农业科学院动物科学研究所 一种初生仔猪肠神经胶质细胞的分离和原代培养方法
CN115591025B (zh) * 2022-11-09 2024-01-02 深圳先进技术研究院 神经调控器件、制备方法及其应用
KR20250108745A (ko) 2022-12-02 2025-07-15 누와셀 바이오테크놀로지 코., 엘티디. 중뇌 도파민 작동성 전구 세포의 제조를 위한 배양 배지, 코팅 매트릭스 및 방법

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6277820B1 (en) * 1998-04-09 2001-08-21 Genentech, Inc. Method of dopaminergic and serotonergic neuron formation from neuroprogenitor cells
US7250294B2 (en) * 2000-05-17 2007-07-31 Geron Corporation Screening small molecule drugs using neural cells differentiated from human embryonic stem cells
JP5943533B2 (ja) * 2000-05-17 2016-07-06 アステリアス バイオセラピューティクス インコーポレイテッド 神経前駆細胞の集団
WO2002086073A2 (en) * 2001-04-20 2002-10-31 Memorial Sloan-Kettering Cancer Center Generation of differentiated tissue from nuclear transfer embryonic stem cells and methods of use

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CN1894401B (zh) 2012-05-23
JP2006521807A (ja) 2006-09-28
ZA200401646B (en) 2004-06-07

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