JP4651282B2 - 造血幹細胞及び造血前駆細胞の増幅方法 - Google Patents

造血幹細胞及び造血前駆細胞の増幅方法 Download PDF

Info

Publication number
JP4651282B2
JP4651282B2 JP2004013291A JP2004013291A JP4651282B2 JP 4651282 B2 JP4651282 B2 JP 4651282B2 JP 2004013291 A JP2004013291 A JP 2004013291A JP 2004013291 A JP2004013291 A JP 2004013291A JP 4651282 B2 JP4651282 B2 JP 4651282B2
Authority
JP
Japan
Prior art keywords
cells
serum
hematopoietic
hematopoietic stem
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2004013291A
Other languages
English (en)
Japanese (ja)
Other versions
JP2005204539A (ja
JP2005204539A5 (OSRAM
Inventor
麻子 初山
仁也 伊藤
龍俊 中畑
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Tanabe Pharma Corp
Foundation for Biomedical Research and Innovation at Kobe
Original Assignee
Mitsubishi Tanabe Pharma Corp
Foundation for Biomedical Research and Innovation at Kobe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Tanabe Pharma Corp, Foundation for Biomedical Research and Innovation at Kobe filed Critical Mitsubishi Tanabe Pharma Corp
Priority to JP2004013291A priority Critical patent/JP4651282B2/ja
Priority to US10/587,014 priority patent/US8168429B2/en
Priority to PCT/JP2004/010677 priority patent/WO2005071064A1/ja
Priority to CA2554192A priority patent/CA2554192C/en
Publication of JP2005204539A publication Critical patent/JP2005204539A/ja
Publication of JP2005204539A5 publication Critical patent/JP2005204539A5/ja
Application granted granted Critical
Publication of JP4651282B2 publication Critical patent/JP4651282B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2004013291A 2004-01-21 2004-01-21 造血幹細胞及び造血前駆細胞の増幅方法 Expired - Fee Related JP4651282B2 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2004013291A JP4651282B2 (ja) 2004-01-21 2004-01-21 造血幹細胞及び造血前駆細胞の増幅方法
US10/587,014 US8168429B2 (en) 2004-01-21 2004-07-21 Method of amplifying hematopoietic stem cell and hematopoietic progenitor cell
PCT/JP2004/010677 WO2005071064A1 (ja) 2004-01-21 2004-07-21 造血幹細胞及び造血前駆細胞の増幅方法
CA2554192A CA2554192C (en) 2004-01-21 2004-07-21 Method of amplifying hematopoietic stem cell and hematopoietic progenitor cell

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2004013291A JP4651282B2 (ja) 2004-01-21 2004-01-21 造血幹細胞及び造血前駆細胞の増幅方法

Publications (3)

Publication Number Publication Date
JP2005204539A JP2005204539A (ja) 2005-08-04
JP2005204539A5 JP2005204539A5 (OSRAM) 2007-03-08
JP4651282B2 true JP4651282B2 (ja) 2011-03-16

Family

ID=34805380

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2004013291A Expired - Fee Related JP4651282B2 (ja) 2004-01-21 2004-01-21 造血幹細胞及び造血前駆細胞の増幅方法

Country Status (4)

Country Link
US (1) US8168429B2 (OSRAM)
JP (1) JP4651282B2 (OSRAM)
CA (1) CA2554192C (OSRAM)
WO (1) WO2005071064A1 (OSRAM)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200530269A (en) 2003-12-12 2005-09-16 Chugai Pharmaceutical Co Ltd Anti-Mpl antibodies
JP4921083B2 (ja) * 2005-09-13 2012-04-18 タカラバイオ株式会社 レトロウィルス産生用無血清培地
KR20090021217A (ko) * 2006-06-14 2009-02-27 추가이 세이야쿠 가부시키가이샤 조혈 줄기세포 증가 촉진제
WO2008009642A1 (en) * 2006-07-17 2008-01-24 Novozymes A/S Cell culture media
PT2194121T (pt) 2007-09-11 2016-12-05 Univ Sapporo Medical Método de proliferação celular e agente farmacêutico para reparação e regeneração de tecidos
CA2708050C (en) * 2007-12-06 2016-08-16 Nissan Chemical Industries, Ltd. Method for expanding hematopoietic stem cells using heterocyclic compound
US20110158955A1 (en) 2008-03-11 2011-06-30 Yoshimoto Katsura Method for producing cells having characteristic of hematopoietic stem cells/progenitor cells
CA2760655C (en) * 2009-06-04 2018-03-06 Nissan Chemical Industries, Ltd. Heterocyclic compounds and expansion agents for hematopoietic stem cells
US9315776B2 (en) 2011-11-09 2016-04-19 National University Of Singapore Wharton's jelly mesenchymal stem cells and uses thereof
SG10201701112QA (en) 2012-08-15 2017-04-27 Univ Singapore Wound dressing nanomesh impregnated with human umbilical cord wharton's jelly stem cells
US9402388B2 (en) * 2012-11-01 2016-08-02 National University Of Singapore Methods of freezing stem cells
EP3201322A4 (en) * 2014-10-03 2018-05-30 Yale University Innate immune system modification for anticancer therapy
TWI548748B (zh) * 2015-04-16 2016-09-11 台灣尖端先進生技醫藥股份有限公司 造血幹／先驅細胞之體外放大培養方法及其立即可使用之組成物
US20180163177A1 (en) * 2016-12-11 2018-06-14 Healthbanks Biotech Co. Ltd. Serum-free culture medium and method for expanding hematopoietic stem cells
CN114686432B (zh) * 2020-12-28 2024-02-02 苏州方舟生物科技有限公司 一种中性粒细胞的高效扩增培养体系及其应用
WO2023275880A1 (en) * 2021-06-30 2023-01-05 Regrow Biosciences Private Limited Method of obtaining hsc population, t cell population and nk cell population and compositions thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL66614A (en) * 1981-08-28 1985-09-29 Genentech Inc Method of constructing a dna sequence encoding a polypeptide,microbial production of human serum albumin,and pharmaceutical compositions comprising it
US5635386A (en) * 1989-06-15 1997-06-03 The Regents Of The University Of Michigan Methods for regulating the specific lineages of cells produced in a human hematopoietic cell culture
US5437994A (en) * 1989-06-15 1995-08-01 Regents Of The University Of Michigan Method for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultures, and for increasing the metabolism, GM-CSF secretion and/or IL-6 secretion of human stromal cells
US5405772A (en) * 1993-06-18 1995-04-11 Amgen Inc. Medium for long-term proliferation and development of cells
AU702871B2 (en) * 1993-08-23 1999-03-11 Nexell Therapeutics Inc. (In vitro) growth of neutrophil and megakaryocyte precursors in serum-free media
US6037174A (en) * 1993-08-23 2000-03-14 Nexell Therapeutics, Inc. Preparation of serum-free suspensions of human hematopoietic cells or precursor cells
US5505908A (en) * 1994-09-01 1996-04-09 Halozone Technologies, Inc. Recycling and recovery of methyl bromide fumigant
EP0891419A4 (en) 1996-03-12 2000-03-01 Life Technologies Inc NUTRIENT ADDITIVE FOR HEMATOPOETIC CELL CULTURES
EP0937461B1 (en) * 1996-07-10 2005-07-06 Meiji Dairies Corporation Use of proteins from the mk family as hematopoietic factor
EP0947581A4 (en) 1996-08-08 2004-07-28 Mitsubishi Pharma Corp CULTURAL MEDIUM AND ITS USE.
US5945337A (en) * 1996-10-18 1999-08-31 Quality Biological, Inc. Method for culturing CD34+ cells in a serum-free medium
AU2003273332A1 (en) * 2002-09-26 2004-04-19 Children's Medical Center Corporation Method of enhancing proliferation and/or hematopoietic differentiation of stem cells
WO2005070120A2 (en) * 2004-01-09 2005-08-04 Serologicals Investment Company, Inc. Cell culture media

Also Published As

Publication number Publication date
US20070166825A1 (en) 2007-07-19
JP2005204539A (ja) 2005-08-04
CA2554192C (en) 2013-09-10
US8168429B2 (en) 2012-05-01
WO2005071064A1 (ja) 2005-08-04
CA2554192A1 (en) 2005-08-04

Similar Documents

Publication Publication Date Title
JP4651282B2 (ja) 造血幹細胞及び造血前駆細胞の増幅方法
US20200263132A1 (en) Methods for producing enucleated erythroid cells derived from pluripotent stem cells
KR102510368B1 (ko) 혈관 콜로니 형성 세포
CN102144028B (zh) 造血前体的扩增
JP7779519B2 (ja) ヒト造血幹細胞などの血球系細胞を培養するために適した無血清培地および培養方法
JPH06508987A (ja) 造血細胞を支持するシステム及び方法
JP7713233B2 (ja) ヒト造血幹細胞を培養するために適したアルブミンフリーの無血清培地およびアルブミンフリーの培養方法
WO1994016715A1 (en) Selective cell proliferation
EP1857542A1 (en) Method for in vitro amplification of adult stem cells
CN115651903B (zh) 高杀伤力的免疫细胞群及其培养方法、试剂组合物和应用
JP5717030B2 (ja) 有核赤血球の脱核方法及び脱核誘導剤
CN112080469B (zh) T1肽在体外促进脐带血造血干细胞增殖中的应用
JP6817633B2 (ja) 造血幹細胞増幅誘導剤
TW202242096A (zh) 用於擴增並維持ＨＳＣｓ自我更新能力和分化潛能的培養基組合物及其應用
CN1310672C (zh) 作为造血刺激物的成骨生长寡肽
WO2025028606A1 (ja) ヒト細胞用培養培地及びヒト造血幹細胞の培養方法
HK40078066A (en) Serum-free medium and culturing method suited for culturing blood cells such as human hematopoietic stem cells
WO2014181783A1 (ja) 赤芽球の脱核方法及び脱核赤血球の維持方法
JP2022017977A (ja) 細胞、組成物及び治療用組成物
JP2003289856A (ja) 造血細胞培養用培地

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20070119

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20070119

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A712

Effective date: 20071102

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20091124

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20100125

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20100223

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20100521

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20100521

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20100625

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20101005

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20101022

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20101207

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20101214

R150 Certificate of patent or registration of utility model

Ref document number: 4651282

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20131224

Year of fee payment: 3

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

S531 Written request for registration of change of domicile

Free format text: JAPANESE INTERMEDIATE CODE: R313531

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees