JP4634799B2 - 耐熱性逆転写酵素およびその使用法 - Google Patents
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- C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
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| US20040152072A1 (en) * | 2002-07-30 | 2004-08-05 | Invitrogen Corporation | Reverse transcription |
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| US7595179B2 (en) | 2004-04-19 | 2009-09-29 | Applied Biosystems, Llc | Recombinant reverse transcriptases |
| US9783791B2 (en) | 2005-08-10 | 2017-10-10 | Agilent Technologies, Inc. | Mutant reverse transcriptase and methods of use |
| ATE535603T1 (de) * | 2005-08-10 | 2011-12-15 | Stratagene California | Mutante reverse transkriptase und verwendungsverfahren |
| US8374838B2 (en) * | 2007-10-22 | 2013-02-12 | The Wistar Institute | Method for identifying a compound that modulates telomerase activity |
| GB0806562D0 (en) * | 2008-04-10 | 2008-05-14 | Fermentas Uab | Production of nucleic acid |
| DK2501716T3 (en) * | 2009-11-19 | 2015-04-07 | Solis Biodyne Oü | Formations to increase polypeptidstabilitet and activity and related practices |
| KR101818126B1 (ko) * | 2011-02-09 | 2018-01-15 | (주)바이오니아 | 열안정성이 증가된 역전사효소 |
| US11465785B1 (en) * | 2012-11-29 | 2022-10-11 | David M. Dodgen | Quick filling and self sealing fluid storage bag assembly |
| JP6221374B2 (ja) * | 2012-12-27 | 2017-11-01 | 東ソー株式会社 | 熱安定性が向上したトリ骨髄芽細胞腫ウイルス逆転写酵素 |
| US9353409B2 (en) * | 2013-01-15 | 2016-05-31 | Jun Euihum Lee | Compositions and methods for RT-PCR |
| EP3043827B1 (en) * | 2013-09-13 | 2019-07-03 | Ionis Pharmaceuticals, Inc. | Modulators of complement factor b |
| JP6769869B2 (ja) * | 2013-10-16 | 2020-10-14 | ニユー・イングランド・バイオレイブス・インコーポレイテツド | 強化された特性を有する逆転写酵素 |
| CN106164261A (zh) | 2014-01-22 | 2016-11-23 | 生命技术公司 | 适用于高温核酸合成的新颖逆转录酶 |
| EP3443079A1 (en) * | 2016-04-12 | 2019-02-20 | Solis Biodyne | Synthetic reverse transcriptases and uses thereof |
| EP3940066A1 (en) * | 2016-09-23 | 2022-01-19 | New England Biolabs, Inc. | A mutant reverse transcriptase |
| KR102489902B1 (ko) * | 2016-11-11 | 2023-01-19 | 바이오 래드 래버러토리스 인코오포레이티드 | 핵산 샘플을 프로세싱하는 방법 |
| WO2018110595A1 (ja) | 2016-12-14 | 2018-06-21 | タカラバイオ株式会社 | 耐熱性の逆転写酵素変異体 |
| US11236384B2 (en) | 2017-01-19 | 2022-02-01 | Asuragen, Inc. | Methods of RNA amplification |
| WO2018152097A1 (en) * | 2017-02-16 | 2018-08-23 | Bio-Rad Laboratories, Inc. | Novel reverse transcriptases and uses thereof |
| US11802311B2 (en) | 2018-03-15 | 2023-10-31 | Massachusetts Institute Of Technology | Methods of quantifying RNA and DNA variants through sequencing employing phosphorothioates |
| CN112534049B (zh) * | 2018-05-16 | 2024-11-22 | 生物辐射实验室股份有限公司 | 处理核酸样本的方法 |
| EP3805380B1 (en) | 2019-10-07 | 2025-12-03 | Qiagen Beverly, LLC | Improved thermostable viral reverse transcriptase |
| EP4073241A2 (en) | 2019-12-11 | 2022-10-19 | 10X Genomics, Inc. | Reverse transcriptase variants |
| CA3186660A1 (en) | 2020-07-20 | 2022-01-27 | Sarah Franz BEAUDOIN | Reverse transcriptase mutants with increased activity and thermostability |
| CN113174381B (zh) * | 2021-06-08 | 2023-05-12 | 翌圣生物科技(上海)股份有限公司 | 增强型mmlv逆转录酶突变体及其应用 |
| US20230392198A1 (en) * | 2022-06-01 | 2023-12-07 | Bio-Rad Laboratories, Inc. | Method and system for detecting reverse transcriptase activity by digital assay |
| WO2024064136A1 (en) * | 2022-09-19 | 2024-03-28 | Domus Diagnostics, Inc. | Rapid molecular diagnostics with unified-one-pot sample processing, nucleic acid amplification, and result readout |
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| US5436149A (en) | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
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| EP1266024A4 (en) * | 2000-03-15 | 2003-07-30 | Invitrogen Corp | HIGH FIDELITY INVERTED TRANSCRIPTASES AND USES THEREOF |
| EP2295551B1 (en) * | 2000-05-26 | 2016-04-06 | Life Technologies Corporation | Thermostable reverse transcriptases and uses thereof |
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|---|---|
| US20140363854A1 (en) | 2014-12-11 |
| US20120021464A1 (en) | 2012-01-26 |
| EP1554377A2 (en) | 2005-07-20 |
| US20040209276A1 (en) | 2004-10-21 |
| EP1554377B1 (en) | 2012-11-07 |
| AU2003274973A1 (en) | 2004-04-30 |
| WO2004024749A3 (en) | 2005-05-19 |
| US20070141592A1 (en) | 2007-06-21 |
| EP2325303A2 (en) | 2011-05-25 |
| EP2325303A3 (en) | 2011-08-17 |
| EP2325303B1 (en) | 2013-11-06 |
| AU2003274973A8 (en) | 2004-04-30 |
| US8753845B2 (en) | 2014-06-17 |
| WO2004024749A2 (en) | 2004-03-25 |
| EP1554377A4 (en) | 2008-03-19 |
| US9862938B2 (en) | 2018-01-09 |
| JP2005538719A (ja) | 2005-12-22 |
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