AU2003274973A1 - Thermostable reverse transcriptases and uses thereof - Google Patents

Thermostable reverse transcriptases and uses thereof

Info

Publication number
AU2003274973A1
AU2003274973A1 AU2003274973A AU2003274973A AU2003274973A1 AU 2003274973 A1 AU2003274973 A1 AU 2003274973A1 AU 2003274973 A AU2003274973 A AU 2003274973A AU 2003274973 A AU2003274973 A AU 2003274973A AU 2003274973 A1 AU2003274973 A1 AU 2003274973A1
Authority
AU
Australia
Prior art keywords
reverse transcriptases
thermostable reverse
thermostable
transcriptases
reverse
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003274973A
Other languages
English (en)
Other versions
AU2003274973A8 (en
Inventor
Gulshan Dhariwal
Gary F. Gerard
Jun E. Lee
Robert Jason Potter
Kim Rosenthal
Michael D. Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Publication of AU2003274973A1 publication Critical patent/AU2003274973A1/en
Publication of AU2003274973A8 publication Critical patent/AU2003274973A8/xx
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2003274973A 2002-09-13 2003-09-15 Thermostable reverse transcriptases and uses thereof Abandoned AU2003274973A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US41028302P 2002-09-13 2002-09-13
US60/410,283 2002-09-13
PCT/US2003/028802 WO2004024749A2 (en) 2002-09-13 2003-09-15 Thermostable reverse transcriptases and uses thereof

Publications (2)

Publication Number Publication Date
AU2003274973A1 true AU2003274973A1 (en) 2004-04-30
AU2003274973A8 AU2003274973A8 (en) 2004-04-30

Family

ID=31994103

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003274973A Abandoned AU2003274973A1 (en) 2002-09-13 2003-09-15 Thermostable reverse transcriptases and uses thereof

Country Status (5)

Country Link
US (4) US20040209276A1 (enExample)
EP (2) EP2325303B1 (enExample)
JP (1) JP4634799B2 (enExample)
AU (1) AU2003274973A1 (enExample)
WO (1) WO2004024749A2 (enExample)

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EP2325303B1 (en) 2002-09-13 2013-11-06 Life Technologies Corporation Thermostable reverse transcriptases and uses thereof
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US9783791B2 (en) 2005-08-10 2017-10-10 Agilent Technologies, Inc. Mutant reverse transcriptase and methods of use
ATE535603T1 (de) * 2005-08-10 2011-12-15 Stratagene California Mutante reverse transkriptase und verwendungsverfahren
US8374838B2 (en) * 2007-10-22 2013-02-12 The Wistar Institute Method for identifying a compound that modulates telomerase activity
GB0806562D0 (en) * 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
DK2501716T3 (en) * 2009-11-19 2015-04-07 Solis Biodyne Oü Formations to increase polypeptidstabilitet and activity and related practices
KR101818126B1 (ko) * 2011-02-09 2018-01-15 (주)바이오니아 열안정성이 증가된 역전사효소
US11465785B1 (en) * 2012-11-29 2022-10-11 David M. Dodgen Quick filling and self sealing fluid storage bag assembly
JP6221374B2 (ja) * 2012-12-27 2017-11-01 東ソー株式会社 熱安定性が向上したトリ骨髄芽細胞腫ウイルス逆転写酵素
US9353409B2 (en) * 2013-01-15 2016-05-31 Jun Euihum Lee Compositions and methods for RT-PCR
EP3043827B1 (en) * 2013-09-13 2019-07-03 Ionis Pharmaceuticals, Inc. Modulators of complement factor b
JP6769869B2 (ja) * 2013-10-16 2020-10-14 ニユー・イングランド・バイオレイブス・インコーポレイテツド 強化された特性を有する逆転写酵素
CN106164261A (zh) 2014-01-22 2016-11-23 生命技术公司 适用于高温核酸合成的新颖逆转录酶
EP3443079A1 (en) * 2016-04-12 2019-02-20 Solis Biodyne Synthetic reverse transcriptases and uses thereof
EP3940066A1 (en) * 2016-09-23 2022-01-19 New England Biolabs, Inc. A mutant reverse transcriptase
KR102489902B1 (ko) * 2016-11-11 2023-01-19 바이오 래드 래버러토리스 인코오포레이티드 핵산 샘플을 프로세싱하는 방법
WO2018110595A1 (ja) 2016-12-14 2018-06-21 タカラバイオ株式会社 耐熱性の逆転写酵素変異体
US11236384B2 (en) 2017-01-19 2022-02-01 Asuragen, Inc. Methods of RNA amplification
WO2018152097A1 (en) * 2017-02-16 2018-08-23 Bio-Rad Laboratories, Inc. Novel reverse transcriptases and uses thereof
US11802311B2 (en) 2018-03-15 2023-10-31 Massachusetts Institute Of Technology Methods of quantifying RNA and DNA variants through sequencing employing phosphorothioates
CN112534049B (zh) * 2018-05-16 2024-11-22 生物辐射实验室股份有限公司 处理核酸样本的方法
EP3805380B1 (en) 2019-10-07 2025-12-03 Qiagen Beverly, LLC Improved thermostable viral reverse transcriptase
EP4073241A2 (en) 2019-12-11 2022-10-19 10X Genomics, Inc. Reverse transcriptase variants
CA3186660A1 (en) 2020-07-20 2022-01-27 Sarah Franz BEAUDOIN Reverse transcriptase mutants with increased activity and thermostability
CN113174381B (zh) * 2021-06-08 2023-05-12 翌圣生物科技(上海)股份有限公司 增强型mmlv逆转录酶突变体及其应用
US20230392198A1 (en) * 2022-06-01 2023-12-07 Bio-Rad Laboratories, Inc. Method and system for detecting reverse transcriptase activity by digital assay
WO2024064136A1 (en) * 2022-09-19 2024-03-28 Domus Diagnostics, Inc. Rapid molecular diagnostics with unified-one-pot sample processing, nucleic acid amplification, and result readout

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US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
DE229810T1 (de) 1985-07-09 1987-11-05 Quadrant Bioresources Ltd., Soulbury, Leighton Buzzard, Bedfordshire Beschuetzung von proteinen und aehnlichem.
US5017492A (en) 1986-02-27 1991-05-21 Life Technologies, Inc. Reverse transcriptase and method for its production
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US4962022A (en) 1986-09-22 1990-10-09 Becton Dickinson And Company Storage and use of liposomes
US5244797B1 (en) * 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5498523A (en) 1988-07-12 1996-03-12 President And Fellows Of Harvard College DNA sequencing with pyrophosphatase
GB8903593D0 (en) 1989-02-16 1989-04-05 Pafra Ltd Storage of materials
ES2134198T3 (es) 1990-09-28 1999-10-01 Hoffmann La Roche Mutaciones en la 5' a 3' exonucleasa de las adn polimerasas.
DE553264T1 (de) 1990-10-05 1994-04-28 Wayne M Barnes Thermostabile dna polymerase.
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US5646016A (en) 1991-02-06 1997-07-08 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules
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US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
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US6989259B2 (en) 1997-04-22 2006-01-24 Invitrogen Corporation Recombinant methods for making reverse transcriptases and mutants thereof
EP0981535A4 (en) 1997-05-12 2000-11-29 Life Technologies Inc PROCESSES FOR PRODUCING AND PURIFYING NUCLEIC ACID MOLECULES
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US6399334B1 (en) 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
US6136582A (en) * 1998-01-20 2000-10-24 The Trustees Of Columbia University In The City Reverse transcriptase of moloney murine leukemia virus with RNA polymerase activity
US6183998B1 (en) 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
JP4193079B2 (ja) 1998-11-10 2008-12-10 東洋紡績株式会社 変異型逆転写酵素
EP1266024A4 (en) * 2000-03-15 2003-07-30 Invitrogen Corp HIGH FIDELITY INVERTED TRANSCRIPTASES AND USES THEREOF
EP2295551B1 (en) * 2000-05-26 2016-04-06 Life Technologies Corporation Thermostable reverse transcriptases and uses thereof
US7078208B2 (en) * 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
JP2004502443A (ja) * 2000-07-12 2004-01-29 インヴィトロジェン　コーポレーション 高忠実度ポリメラーゼおよびその使用方法
EP2325303B1 (en) 2002-09-13 2013-11-06 Life Technologies Corporation Thermostable reverse transcriptases and uses thereof
GB0806562D0 (en) 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid

Also Published As

Publication number Publication date
US20140363854A1 (en) 2014-12-11
US20120021464A1 (en) 2012-01-26
EP1554377A2 (en) 2005-07-20
US20040209276A1 (en) 2004-10-21
EP1554377B1 (en) 2012-11-07
WO2004024749A3 (en) 2005-05-19
US20070141592A1 (en) 2007-06-21
EP2325303A2 (en) 2011-05-25
EP2325303A3 (en) 2011-08-17
EP2325303B1 (en) 2013-11-06
AU2003274973A8 (en) 2004-04-30
US8753845B2 (en) 2014-06-17
WO2004024749A2 (en) 2004-03-25
JP4634799B2 (ja) 2011-02-16
EP1554377A4 (en) 2008-03-19
US9862938B2 (en) 2018-01-09
JP2005538719A (ja) 2005-12-22

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase