JP4527619B2 - 液体クロマトグラフィーをキャピラリー電気泳動に接続する予備濃縮インターフェース - Google Patents

液体クロマトグラフィーをキャピラリー電気泳動に接続する予備濃縮インターフェース Download PDF

Info

Publication number
JP4527619B2
JP4527619B2 JP2005193598A JP2005193598A JP4527619B2 JP 4527619 B2 JP4527619 B2 JP 4527619B2 JP 2005193598 A JP2005193598 A JP 2005193598A JP 2005193598 A JP2005193598 A JP 2005193598A JP 4527619 B2 JP4527619 B2 JP 4527619B2
Authority
JP
Japan
Prior art keywords
separation
cze
fraction
electrodes
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2005193598A
Other languages
English (en)
Japanese (ja)
Other versions
JP2006017731A (ja
JP2006017731A5 (enExample
Inventor
クルチオ マリオ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of JP2006017731A publication Critical patent/JP2006017731A/ja
Publication of JP2006017731A5 publication Critical patent/JP2006017731A5/ja
Application granted granted Critical
Publication of JP4527619B2 publication Critical patent/JP4527619B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G01N30/463—Flow patterns using more than one column with serial coupling of separation columns for multidimensional chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/84—Preparation of the fraction to be distributed
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G01N30/465—Flow patterns using more than one column with serial coupling of separation columns with specially adapted interfaces between the columns

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Pyridine Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Electrochromic Elements, Electrophoresis, Or Variable Reflection Or Absorption Elements (AREA)
JP2005193598A 2004-07-03 2005-07-01 液体クロマトグラフィーをキャピラリー電気泳動に接続する予備濃縮インターフェース Expired - Fee Related JP4527619B2 (ja)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP04015701A EP1612550B1 (en) 2004-07-03 2004-07-03 Preconcentration interface coupling liquid chomatography to capillary electrophoresis

Publications (3)

Publication Number Publication Date
JP2006017731A JP2006017731A (ja) 2006-01-19
JP2006017731A5 JP2006017731A5 (enExample) 2006-03-16
JP4527619B2 true JP4527619B2 (ja) 2010-08-18

Family

ID=34925611

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2005193598A Expired - Fee Related JP4527619B2 (ja) 2004-07-03 2005-07-01 液体クロマトグラフィーをキャピラリー電気泳動に接続する予備濃縮インターフェース

Country Status (7)

Country Link
US (1) US7820023B2 (enExample)
EP (2) EP1717580B1 (enExample)
JP (1) JP4527619B2 (enExample)
AT (2) ATE388397T1 (enExample)
CA (1) CA2511079A1 (enExample)
DE (2) DE602004012326T2 (enExample)
ES (2) ES2283907T3 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7641780B2 (en) * 2001-05-01 2010-01-05 Calibrant Biosystems, Inc. Two-dimensional microfluidics for protein separations and gene analysis
US20080156080A1 (en) * 2007-01-02 2008-07-03 Calibrant Biosystems, Inc. Methods and systems for multidimensional concentration and separation of biomolecules using capillary isotachophoresis
US20080160629A1 (en) * 2007-01-02 2008-07-03 Calibrant Biosystems, Inc. Methods and systems for off-line multidimensional concentration and separation of biomolecules
WO2009042921A1 (en) * 2007-09-26 2009-04-02 Massachusetts Institute Of Technology Electrokinetic concentration device and methods of use thereof
JP5047928B2 (ja) * 2008-11-11 2012-10-10 シャープ株式会社 電気泳動装置およびその構成器具
CZ303056B6 (cs) 2010-12-14 2012-03-14 Mikrobiologický ústav AV CR, v.v.i. Zpusob modifikace povrchu pro prekoncentraci fosforylovaných peptidu pro desorpcne-ionizacní techniky hmotnostní spektrometrie
US8871072B2 (en) * 2011-09-28 2014-10-28 The United States Of America, As Represented By The Secretary Of The Navy Flow step focusing
CZ307023B6 (cs) * 2013-05-20 2017-11-22 Ăšstav analytickĂ© chemie AV ÄŚR, v. v. i. Způsob předkoncentrace složek kapalných vzorků
CN103638691B (zh) * 2013-11-01 2015-07-22 朱江 混合物分离纯化用交变弱电场阵列电子色谱分离方法
CN110114667B (zh) * 2016-12-23 2023-07-25 豪夫迈·罗氏有限公司 用于在分析系统中的过程期间标识试剂的方法
JP7051871B2 (ja) 2016-12-23 2022-04-11 エフ．ホフマン－ラ ロシュ アーゲー 分析系におけるプロセス中に試薬を同定するための方法
CN112354572B (zh) * 2019-07-11 2022-02-11 北京理工大学 一种多维微流控电泳芯片及检测装置，检测方法
CN112098501B (zh) * 2020-09-15 2023-12-05 中国科学院合肥物质科学研究院 一种面向高毒性VOCs混合物现场检测的nafion-FAIMS检测装置及方法

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3735640A (en) 1972-03-10 1973-05-29 L Chizhov Apparatus for injecting a sample into a gas chromatograph
US4043678A (en) 1976-03-01 1977-08-23 Technicon Instruments Corporation Cuvette
GB2225339A (en) * 1988-11-15 1990-05-30 Aligena Ag Separating electrically charged macromolecular compounds by forced-flow membrane electrophoresis
US5240577A (en) * 1990-11-13 1993-08-31 University Of North Carolina At Chapel Hill Two-dimensional high-performance liquid chromatography/capillary electrophoresis
EP0488947B1 (de) 1990-11-26 1995-02-15 Ciba-Geigy Ag Detektorzelle
US6074827A (en) * 1996-07-30 2000-06-13 Aclara Biosciences, Inc. Microfluidic method for nucleic acid purification and processing
US6387234B1 (en) * 1998-08-31 2002-05-14 Iowa State University Research Foundation, Inc. Integrated multiplexed capillary electrophoresis system
WO2001053794A1 (en) 2000-01-18 2001-07-26 Northeastern University Parallel sample loading and injection device for multichannel microfluidic devices
US6749735B1 (en) * 2000-03-16 2004-06-15 David Le Febre Electromobility focusing controlled channel electrophoresis system
US6558523B1 (en) * 2000-04-10 2003-05-06 The Regents Of The University Of California Injector-concentrator electrodes for microchannel electrophoresis
JP3744310B2 (ja) 2000-04-13 2006-02-08 株式会社日立製作所 電気泳動装置
DE60138824D1 (de) * 2000-10-31 2009-07-09 Caliper Life Sciences Inc Mikrofluidisches verfahren für in-situ-materialkonzentration
US7070682B2 (en) * 2001-01-16 2006-07-04 Cheng Lee Microfluidic apparatus for performing gel protein extractions and methods for using the apparatus
KR100425536B1 (ko) 2001-07-16 2004-03-30 학교법인 포항공과대학교 유체 마이크로칩용 브레드보드
DE10136275C1 (de) * 2001-07-25 2002-12-12 Fraunhofer Ges Forschung Verfahren und Vorrichtung zur Probentrennung
JP2003066005A (ja) * 2001-08-23 2003-03-05 Kikuchi Jun 細胞の電気泳動方法及び装置
JP2003149218A (ja) 2001-11-08 2003-05-21 Nano Solution:Kk ２次元高速液体クロマトグラフ装置及びそれを用いた蛋白質分析装置
US6802967B2 (en) 2002-03-06 2004-10-12 Shimadzu Corporation Multi-dimension liquid chromatography separation system
JP2004101477A (ja) 2002-09-12 2004-04-02 Yoshio Yamauchi ２次元高速液体クロマトグラフ装置及びそれを用いた蛋白質分析装置
JP2004157096A (ja) * 2002-11-02 2004-06-03 Minoru Seki 化学物質の二次元分離機構及びその装置

Also Published As

Publication number Publication date
DE602004005843D1 (de) 2007-05-24
ATE359504T1 (de) 2007-05-15
ES2301118T3 (es) 2008-06-16
ES2283907T3 (es) 2007-11-01
EP1717580B1 (en) 2008-03-05
EP1612550B1 (en) 2007-04-11
JP2006017731A (ja) 2006-01-19
DE602004012326T2 (de) 2009-03-26
DE602004012326D1 (de) 2008-04-17
EP1717580A2 (en) 2006-11-02
EP1612550A1 (en) 2006-01-04
ATE388397T1 (de) 2008-03-15
US20060086611A1 (en) 2006-04-27
DE602004005843T2 (de) 2007-08-30
EP1717580A3 (en) 2006-12-13
CA2511079A1 (en) 2006-01-03
US7820023B2 (en) 2010-10-26

Similar Documents

Publication Publication Date Title
Chien Sample stacking revisited: A personal perspective
Breadmore Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips
Giordano et al. On-line sample pre-concentration in microfluidic devices: A review
Stroink et al. On-line multidimensional liquid chromatography and capillary electrophoresis systems for peptides and proteins
Goetz et al. Comparison of selected analytical techniques for protein sizing, quantitation and molecular weight determination
Ishihama et al. Surfactants usable for electrospray ionization mass spectrometry
JP4527619B2 (ja) 液体クロマトグラフィーをキャピラリー電気泳動に接続する予備濃縮インターフェース
Zhang et al. Integrated strong cation-exchange hybrid monolith coupled with capillary zone electrophoresis and simultaneous dynamic pH junction for large-volume proteomic analysis by mass spectrometry
Yang et al. Two-dimensional capillary electrophoresis involving capillary isoelectric focusing and capillary zone electrophoresis
Astorga-Wells et al. A microfluidic electrocapture device in sample preparation for protein analysis by MALDI mass spectrometry
Benavente et al. Lowering the concentration limits of detection by on-line solid-phase extraction–capillary electrophoresis–electrospray mass spectrometry
WO2008082876A1 (en) Methods and systems for multidimensional concentration and separation of biomolecules using capillary isotachophoresis
Kler et al. Column–coupling strategies for multidimensional electrophoretic separation techniques
Lindenburg et al. The potential of electrophoretic sample pretreatment techniques and new instrumentation for bioanalysis, with a focus on peptidomics and metabolomics
Monton et al. Sample enrichment techniques in capillary electrophoresis: Focus on peptides and proteins
Guzman et al. New directions for concentration sensitivity enhancement in CE and microchip technology
Saavedra et al. Chromatography-based on-and in-line pre-concentration methods in capillary electrophoresis
Wu New approaches to sample preparation for capillary electrophoresis
JP2006017731A5 (enExample)
Tachibana et al. Effects of the length and modification of the separation channel on microchip electrophoresis–mass spectrometry for analysis of bioactive compounds
Ren et al. Preconcentration of 3-nitrotyrosine in urine by transient isotachophoresis in MEKC
CN101271116B (zh) 一种强阴离子预柱在磷酸化肽自动化分析过程中的应用
Carr The role of chromatography in the characterization and analysis of protein therapeutic drugs
Shariatgorji et al. Trends in the bioanalytical applications of microfluidic electrocapture
Mikuš et al. Column coupling electrophoresis in biomedical analysis

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20060127

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20060127

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20080529

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20090120

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20090417

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20091215

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20100312

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20100511

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20100603

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130611

Year of fee payment: 3

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

LAPS Cancellation because of no payment of annual fees