JP4481168B2 - 分析試料内に含まれる少なくとも1つのリガンドを検出する装置 - Google Patents
分析試料内に含まれる少なくとも1つのリガンドを検出する装置 Download PDFInfo
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- JP4481168B2 JP4481168B2 JP2004540719A JP2004540719A JP4481168B2 JP 4481168 B2 JP4481168 B2 JP 4481168B2 JP 2004540719 A JP2004540719 A JP 2004540719A JP 2004540719 A JP2004540719 A JP 2004540719A JP 4481168 B2 JP4481168 B2 JP 4481168B2
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
- G01N21/6454—Individual samples arranged in a regular 2D-array, e.g. multiwell plates using an integrated detector array
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
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- Health & Medical Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Engineering & Computer Science (AREA)
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- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
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- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Macromolecular Compounds Obtained By Forming Nitrogen-Containing Linkages In General (AREA)
Description
Claims (14)
- 分析試料に含まれる少なくとも1つのリガンド(2)を検出する装置(1)において、リガンド(2)と接触した場合にリガンド(2)と特異的結合する、異なるレセプターが直接的又は間接的に固定される複数の検出フィールドが表面に配置される光ウエーブガイド(4)と、レセプター(5)でのリガンド(2)の結合に依存して発光物質(10)のエバネッセント励起のため、光ウエーブガイド(4)内への励起ビーム(9)を接続する少なくとも1つの光ビーム源(8)と、個々の検出フィールドのために、それぞれ、発光物質(10)の局部分解の検出のため、半導体チップ上に配置される少なくとも1つのビーム受信器(12)を有する半導体チップ(3)とを備え、ウエーブガイド(4)は半導体基板とモノリシックに集積され、又はウエーブガイド層として半導体チップ(3)上に配置され、検出フィールドに付設されるビーム受信器(12)は検出フィールドに対向するウエーブガイドの裏側において半導体基板内で並列して直接に配置されることを特徴とする分析試料に含まれる少なくとも1つのリガンドを検出する装置。
- 半導体チップ(3)の地形は望ましくない光離脱を避けるため、少なくとも1つのレセプター(5)に対向する境界面(14)が半導体チップ(3)とウエーブガイド(4)との間で、半導体チップ(3)の延長面に対して平行に配置される2つの平面(14a, 14b)間で走り、2つの平面の間隔(x)は励起ビーム(9)の波長より小さいことを特徴とする請求項1記載の装置(1)。
- 半導体チップ(3)は、電子回路のためにウエーブガイド(4)と横に並んだ構造部(13)を有することを特徴とする請求項1又は2記載の装置(1)。
- 半導体チップ(3)とウエーブガイド(4)との間には中間層(15)が配置され、中間層の光屈折率はウエーブガイド(4)の屈折率より小さく、中間層(15)は半導体チップ(3)に直接隣接する半導体チップ(3)のネガの形状を有し、半導体チップ(3)と対向してウエーブガイド(4)に隣接する中間層(15)の前側で直接に本質的に平坦に構成されることを特徴とする請求項1ないし3いずれか1項に記載の装置(1)。
- 中間層は接着剤層として構成されることを特徴とする請求項1ないし4いずれか1項に記載の装置(1)。
- ウエーブガイド(4)は少なくとも1つの結合個所を介して半導体チップ(3)と接続されることを特徴とする請求項1ないし5いずれか1項に記載の装置(1)。
- ウエーブガイド(4)は、透明な重合体材料から成る薄膜層として構成されることを特徴とする請求項1ないし6いずれか1項に記載の装置(1)。
- ウエーブガイド(4)は金属酸化物により形成されることを特徴とする請求項1ないし7いずれか1項に記載の装置(1)。
- 光ビーム源(8)は半導体ビーム源として構成され、半導体チップ(3)内で一体化されることを特徴とする請求項1ないし8いずれか1項に記載の装置(1)。
- ウエーブガイド(4)内への励起ビーム(9)の接続のため、光ビーム源(8)の放射範囲内で接続光学系(11)が設けられ、接続光学系は、ウエーブガイド(4)と一体に構成され、プリズム、光格子及び転向ミラー或いはそれらの内の一方を有することを特徴とする請求項1ないし9いずれか1項に記載の装置(1)。
- 個々のビーム受信器(12)が、本質的に他のビーム受信器(12)の検出フィールドの発光ビームを受信しないように、検出フィールドは相互に離間され、ビーム受信器(12)に関して配置されることを特徴とする請求項1ないし10いずれか1項に記載の装置(1)。
- 少なくとも1つのレセプター(5)は、少なくとも入口(18)と出口(19)とを有する貫流測定室の内部空洞(17)内に配置され、半導体チップ(3)は、貫流測定室の壁範囲を形成することを請求項1ないし11いずれか1項に記載の装置(1)。
- 貫流測定室の温度調節のため、ペルチェ素子を有する加熱装置及び冷却装置或いはそれらの内の一方が設けられることを請求項1ないし12いずれか1項に記載の装置(1)。
- 貫流測定室内には、少なくとも1つのレセプター(5)での少なくとも1つのリガンド(2)の結合の検出のため、少なくとも1つの試薬及び反応体或いはそれらの内の一方が貯留されることを特徴とする請求項1ないし13いずれか1項に記載の装置(1)。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10245435A DE10245435B4 (de) | 2002-09-27 | 2002-09-27 | Vorrichtung zur Detektion mindestens eines in einer zu untersuchenden Probe enthaltenen Liganden |
PCT/EP2003/010736 WO2004031750A1 (de) | 2002-09-27 | 2003-09-26 | Vorrichtung zur detektion mindestens eines in einer zu untersuchenden probe enthaltenen liganden |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2006502379A JP2006502379A (ja) | 2006-01-19 |
JP2006502379A5 JP2006502379A5 (ja) | 2009-11-19 |
JP4481168B2 true JP4481168B2 (ja) | 2010-06-16 |
Family
ID=31984235
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2004540719A Expired - Lifetime JP4481168B2 (ja) | 2002-09-27 | 2003-09-26 | 分析試料内に含まれる少なくとも1つのリガンドを検出する装置 |
Country Status (6)
Country | Link |
---|---|
US (1) | US7462326B2 (ja) |
EP (1) | EP1543315B1 (ja) |
JP (1) | JP4481168B2 (ja) |
AT (1) | ATE354084T1 (ja) |
DE (2) | DE10245435B4 (ja) |
WO (1) | WO2004031750A1 (ja) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1784500A1 (de) * | 2004-09-01 | 2007-05-16 | Holger Dr. Klapproth | Verfahren zum analysieren von punktmutationen |
DE102004056787A1 (de) * | 2004-11-24 | 2006-06-01 | Heinrich-Heine-Universität Düsseldorf | Vorrichtung und Verfahren zur Messung von Fluoreszenz in mehreren Reaktionsräumen |
RU2009105884A (ru) * | 2006-07-20 | 2010-08-27 | Конинклейке Филипс Электроникс Н.В. (Nl) | Детекторы излучения, использующие возбуждение рассеянным полем |
EP1980854B1 (de) * | 2007-04-12 | 2009-10-07 | Micronas Holding GmbH | Verfahren und Vorrichtung zur Messung der Konzentration eines in einer zu untersuchenden Probe enthaltenen Liganden |
CN101796394A (zh) * | 2007-07-12 | 2010-08-04 | 纳米识别技术股份公司 | 光电传感器系统 |
CN103954595A (zh) * | 2007-07-12 | 2014-07-30 | 纳米识别技术股份公司 | 光电传感器系统 |
EP2020601B1 (de) * | 2007-08-03 | 2010-11-17 | Micronas GmbH | Kit und Vorrichtung zum Erzeugen einer Chemolumineszenzstrahlung |
AU2011217862B9 (en) | 2010-02-19 | 2014-07-10 | Pacific Biosciences Of California, Inc. | Integrated analytical system and method |
US8837872B2 (en) | 2010-12-30 | 2014-09-16 | Qualcomm Incorporated | Waveguide structures for signal and/or power transmission in a semiconductor device |
WO2013080432A1 (ja) * | 2011-11-28 | 2013-06-06 | ソニー株式会社 | ケミカルセンサ、ケミカルセンサモジュール、化学物質検出装置及び化学物質検出方法 |
GB201207918D0 (en) * | 2012-05-04 | 2012-06-20 | Univ Lincoln The | Detector apparatus and method |
CA3178340A1 (en) | 2012-08-20 | 2014-02-27 | Illumina, Inc. | Method and system for fluorescence lifetime based sequencing |
DE102014213440A1 (de) | 2014-07-10 | 2016-01-14 | Robert Bosch Gmbh | Verfahren zur Herstellung einer Komplettisolation |
DE102014221734A1 (de) * | 2014-10-24 | 2016-04-28 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Messvorrichtung und System zur Schmelzkurvenanalyse eines DNA Microarrays, sowie Verwendung eines Fluoreszenzdetektorarrays zur Analyse |
Family Cites Families (20)
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ES2123063T3 (es) | 1992-09-14 | 1999-01-01 | Stanford Res Inst Int | Marcadores convertidores al alza para ensayos biologicos y otros mediante tecnicas de excitacion laser. |
US5439647A (en) * | 1994-02-25 | 1995-08-08 | Fiberchem, Inc. | Chip level waveguide sensor |
US5737457A (en) | 1994-02-25 | 1998-04-07 | Fci - Fiberchem, Inc. | Chip level waveguide sensor |
JP3645907B2 (ja) * | 1994-05-27 | 2005-05-11 | ノバルティス アクチエンゲゼルシャフト | 漸減励起された発光を検出する方法 |
CA2250212C (en) * | 1996-04-03 | 2010-02-09 | The Perkin-Elmer Corporation | Device and method for multiple analyte detection |
US5832165A (en) * | 1996-08-28 | 1998-11-03 | University Of Utah Research Foundation | Composite waveguide for solid phase binding assays |
US5948621A (en) | 1997-09-30 | 1999-09-07 | The United States Of America As Represented By The Secretary Of The Navy | Direct molecular patterning using a micro-stamp gel |
US6893877B2 (en) | 1998-01-12 | 2005-05-17 | Massachusetts Institute Of Technology | Methods for screening substances in a microwell array |
US6287874B1 (en) * | 1998-02-02 | 2001-09-11 | Signature Bioscience, Inc. | Methods for analyzing protein binding events |
CA2318881A1 (en) | 1998-02-04 | 1999-08-12 | Tina Garyantes | Virtual wells for use in high throughput screening assays |
US6187537B1 (en) | 1998-04-27 | 2001-02-13 | Donald E. Zinn, Jr. | Process and apparatus for forming a dry DNA transfer film, a transfer film product formed thereby and an analyzing process using the same |
FR2778986B1 (fr) * | 1998-05-22 | 2000-07-21 | Suisse Electronique Microtech | Capteur optique utilisant une reaction immunologique et un marqueur fluorescent |
US6235473B1 (en) | 1998-07-02 | 2001-05-22 | Orchid Biosciences, Inc. | Gene pen devices for array printing |
US5936730A (en) * | 1998-09-08 | 1999-08-10 | Motorola, Inc. | Bio-molecule analyzer with detector array and filter device |
WO2000017401A2 (en) * | 1998-09-21 | 2000-03-30 | Ramot University Authority For Applied Research & Industrial Development Ltd. | Method and apparatus for effecting and monitoring nucleic acid amplification |
WO2000043539A2 (en) | 1999-01-25 | 2000-07-27 | Biochip Technologies Gmbh | Immobilization of molecules on surfaces via polymer brushes |
US6771376B2 (en) * | 1999-07-05 | 2004-08-03 | Novartis Ag | Sensor platform, apparatus incorporating the platform, and process using the platform |
DE19947616C2 (de) * | 1999-10-01 | 2003-05-28 | Fraunhofer Ges Forschung | Verfahren zur Bestimmung von Substanzen, wie z.B. DNA-Sequenzen, in einer Probe und Vorrichtung zur Durchführung des Verfahrens |
DE10002566A1 (de) | 2000-01-21 | 2001-08-02 | Fraunhofer Ges Forschung | Verfahren und Einrichtung zur Bestimmung des Schmelzpunktes und/oder der Bindungskonstante von Substanzen, wie z. B. DNA-Sequenzen, in einer Probe |
AU2001261094A1 (en) * | 2000-04-28 | 2001-11-12 | Edgelight Biosciences, Inc. | Micro-array evanescent wave fluorescence detection device |
-
2002
- 2002-09-27 DE DE10245435A patent/DE10245435B4/de not_active Expired - Fee Related
-
2003
- 2003-09-26 JP JP2004540719A patent/JP4481168B2/ja not_active Expired - Lifetime
- 2003-09-26 EP EP03753466A patent/EP1543315B1/de not_active Expired - Lifetime
- 2003-09-26 AT AT03753466T patent/ATE354084T1/de not_active IP Right Cessation
- 2003-09-26 WO PCT/EP2003/010736 patent/WO2004031750A1/de active IP Right Grant
- 2003-09-26 US US10/528,954 patent/US7462326B2/en not_active Expired - Fee Related
- 2003-09-26 DE DE50306531T patent/DE50306531D1/de not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JP2006502379A (ja) | 2006-01-19 |
US7462326B2 (en) | 2008-12-09 |
DE10245435B4 (de) | 2006-03-16 |
WO2004031750A1 (de) | 2004-04-15 |
EP1543315A1 (de) | 2005-06-22 |
DE10245435A1 (de) | 2004-04-08 |
DE50306531D1 (de) | 2007-03-29 |
EP1543315B1 (de) | 2007-02-14 |
ATE354084T1 (de) | 2007-03-15 |
US20060051244A1 (en) | 2006-03-09 |
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